Abstract:
:Previous studies have suggested that the Pseudomonas aeruginosa PAK pilus adhesin moiety resides in an epithelial cell-binding domain located in the C-terminal region of the PAK pilin structural protein. Synthetic peptides Ac17red (a synthetic peptide with a sequence identical to that of PAK pilin residues 128 to 144, with the Cys-129 and Cys-142 residues being in the reduced state) and Ac17ox (a synthetic peptide with a sequence identical to that of PAK pilin residues 128 to 144, with a formed disulfide bridge between the amino acid residues Cys-129 and Cys-142), which should contain the epithelial cell-binding domain, were synthesized. Ac17red and Ac17ox both bound to buccal epithelial cells (BECs) and to ciliated tracheal epithelial cells (TECs). Ac17ox had a Km of 6.40 microM for binding to BECs, while Ac17red had a Km of 9.87 microM. Ac17red bound to the same receptor sites that purified pili did and competitively inhibited the binding of purified PAK pili to BECs. BEC glycoproteins with molecular masses of 82, 55 to 51, and 40 kilodaltons immobilized on nitrocellulose exhibited periodate-sensitive receptor activity for Ac17red; similar activity has been found for PAK pili. Ac17red, Ac17ox, and PAK pili bound to the cilia and luminal portions of the cytoplasmic membrane of human TECs, the same regions to which P. aeruginosa whole cells bind. PAK pilin has an epithelial cell-binding domain that resides in the C-terminal region of the protein.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Irvin RT,Doig P,Lee KK,Sastry PA,Paranchych W,Todd T,Hodges RSdoi
10.1128/IAI.57.12.3720-3726.1989subject
Has Abstractpub_date
1989-12-01 00:00:00pages
3720-6issue
12eissn
0019-9567issn
1098-5522journal_volume
57pub_type
杂志文章abstract::The virulence plasmids of Salmonella typhimurium and other invasive Salmonella serovars have long been associated with the ability of these bacteria to cause systemic infection beyond the intestines in orally inoculated animals. Genetic analysis of virulence genes on the high-molecular-weight plasmids has revealed tha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.2.504-511.1993
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.7.1692-1700.1987
更新日期:1987-07-01 00:00:00
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更新日期:1997-10-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.01199-12
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journal_title:Infection and immunity
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doi:10.1128/IAI.47.3.719-722.1985
更新日期:1985-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.1.375-379.1996
更新日期:1996-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.686-691.1987
更新日期:1987-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.1.111-117.1981
更新日期:1981-04-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1995-02-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.33.2.620-622.1981
更新日期:1981-08-01 00:00:00
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pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.3.745-752.1985
更新日期:1985-12-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1993-12-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.33.2.641-642.1981
更新日期:1981-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00290-09
更新日期:2009-09-01 00:00:00
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pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:Infection and immunity
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doi:10.1128/IAI.43.1.54-58.1984
更新日期:1984-01-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.2.647-653.1982
更新日期:1982-02-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.62.3.961-967.1994
更新日期:1994-03-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1995-09-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2007-08-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1998-05-01 00:00:00
abstract::Several pathogenic fungal organisms enter eukaryotic cells and manipulate the host cell environment to favor their own growth and survival. Aspergillus fumigatus is a saprophytic fungus that causes invasive lung disease in the immunocompromised host. To determine whether A. fumigatus could enter eukaryotic cells, we s...
journal_title:Infection and immunity
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更新日期:2002-06-01 00:00:00