Cloning of Syrian hamster (Mesocricetus auratus) cytokine cDNAs and analysis of cytokine mRNA expression in experimental visceral leishmaniasis.

Abstract:

:The Syrian golden hamster (Mesocricetus auratus) is uniquely susceptible to a variety of intracellular pathogens and is an excellent model for a number of human infectious diseases. The molecular basis for this high level of susceptibility is unknown, and immunological studies related to this model have been limited by the lack of available reagents. In this report we describe the cloning and sequence analysis of portions of the Syrian hamster interleukin 2 (IL-2), IL-4, gamma interferon (IFN-gamma), tumor necrosis factor alpha, IL-10, IL-12p40, and transforming growth factor beta cDNAs. In addition, we examined the cytokine response to infection with the intracellular protozoan Leishmania donovani in this animal model. Sequence analysis of the hamster cytokines revealed 69 to 93% homology with the corresponding mouse, rat, and human nucleotide sequences and 48 to 100% homology with the deduced amino acid sequences. The hamster IFN-gamma, compared with the mouse and rat homologs, had an additional 17 amino acids at the C terminus that could decrease the biological activity of this molecule and thus contribute to the extreme susceptibility of this animal to intracellular pathogens. The splenic expression of these genes in response to infection with L. donovani, the cause of visceral leishmaniasis (VL), was determined by Northern blotting. VL in the hamster is a progressive, lethal disease which very closely mimics active human disease. In this model there was pronounced expression of the Th1 cytokine mRNAs, with transcripts being detected as early as 1 week postinfection. Basal expression of IL-4 in uninfected hamsters was prominent but did not increase in response to infection with L. donovani. IL-12 transcript expression was detected at low levels in infected animals and paralleled the expression of IFN-gamma. Expression of IL-10, a potent macrophage deactivator, increased throughout the course of infection and could contribute to the progressive nature of this infection. These initial studies are the first to examine the molecular immunopathogenesis of a hamster model of VL infection and indicate that progressive disease in this model of VL is not associated with early polarization of the splenic cellular immune response toward a Th2 phenotype and away from a Th1 phenotype.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Melby PC,Tryon VV,Chandrasekar B,Freeman GL

doi

10.1128/IAI.66.5.2135-2142.1998

subject

Has Abstract

pub_date

1998-05-01 00:00:00

pages

2135-42

issue

5

eissn

0019-9567

issn

1098-5522

journal_volume

66

pub_type

杂志文章
  • Interaction of lipopolysaccharides of Helicobacter pylori with basement membrane protein laminin.

    abstract::The ability of hemagglutinating and poorly hemagglutinating strains of the gastroduodenal pathogen Helicobacter pylori to bind 125I-radiolabelled laminin was quantitated in a liquid phase assay. Although all strains bound laminin, some hemagglutinating strains were good binders of laminin (maximum of 31% binding), whe...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.9.3640-3648.1994

    authors: Valkonen KH,Wadström T,Moran AP

    更新日期:1994-09-01 00:00:00

  • Levels of salivary lysozyme, lactoperoxidase, and lactoferrin in diabetic hamsters.

    abstract::In an attempt to clarify the mechanism(s) of increased susceptibility to oral infection in diabetics, we examined the levels of salivary antibacterial factors, including lysozyme, lactoperoxidase, and lactoferrin, in diabetic hamsters whose condition was induced with streptozotocin. Saliva was collected from these ham...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.48.2.389-394.1985

    authors: Muratsu K,Morioka T

    更新日期:1985-05-01 00:00:00

  • Prevention of the generalized Shwartzman reaction and endotoxin lethality by polymyxin B localized in tissues.

    abstract::Pretreatment with multiple doses of polymyxin B and colistimethate was evaluated as to its ability to sequester sufficient antibiotic in tissues to neutralize the effects of endotoxin in three animal models. Animals were challenged with endotoxin 24, 48, or 72 h after the last dose of antibiotic when there was minimal...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.10.2.287-292.1974

    authors: Craig WA,Turner JH,Kunin CM

    更新日期:1974-08-01 00:00:00

  • Regulation of hemolysin expression in Actinobacillus pleuropneumoniae serotype 1 by Ca2+.

    abstract::Actinobacillus pleuropneumoniae, the causative agent of swine pleuropneumonia, secretes a hemolytic activity which is thought to be a factor involved in the pathogenesis of the disease. The biosynthesis of hemolysin by serotype 1 strain 4074 was strongly dependent on the activity of free Ca2+ in the growth medium. At ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.56.10.2570-2575.1988

    authors: Frey J,Nicolet J

    更新日期:1988-10-01 00:00:00

  • Depressed specific cell-mediated immunity to Herpes simplex virus type 1 in patients with recurrent herpes labialis.

    abstract::Cell-mediated immunity to herpes simplex virus type 1 (HSV-1) was assessed by a lymphocytotoxicity 51-Cr-release microassay procedure, using the MA-160 human prostatic adenoma cell line chronically infected with HSV-1 and its parent cell line as control. The specific immune release mean plus or minus standard deviatio...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.12.1.76-80.1975

    authors: Thong YH,Vincent MM,Hensen SA,Fuccillo DA,Rola-Pleszczynski M,Bellanti JA

    更新日期:1975-07-01 00:00:00

  • Degradation of immunoglobulins A2, A2, and G by suspected principal periodontal pathogens.

    abstract::Attention has recently been focused on immunoglobulin A1 (IgA1) protease production as a possible virulence factor of bacteria implicated in meningitis and gonorrhea. This report demonstrates that suspected principal etiological agents in destructive periodontal disease include bacteria capable of degrading IgA1, IgA2...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.34.3.757-765.1981

    authors: Kilian M

    更新日期:1981-12-01 00:00:00

  • Intimin, tir, and shiga toxin 1 do not influence enteropathogenic responses to shiga toxin-producing Escherichia coli in bovine ligated intestinal loops.

    abstract::Shiga toxin-producing Escherchia coli (STEC) comprises a group of attaching and effacing (A/E) enteric pathogens of animals and humans. Natural and experimental infection of calves with STEC may result in acute enteritis or subclinical infection, depending on serotype- and host-specific factors. To quantify intestinal...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.70.2.945-952.2002

    authors: Stevens MP,Marchès O,Campbell J,Huter V,Frankel G,Phillips AD,Oswald E,Wallis TS

    更新日期:2002-02-01 00:00:00

  • Helicobacter pylori induces interleukin-8 secretion by Toll-like receptor 2- and Toll-like receptor 5-dependent and -independent pathways.

    abstract::Helicobacter pylori is an important human pathogen that causes gastritis and is strongly associated with gastric ulcers, gastric adenocarcinomas, and mucosa-associated lymphoid tissue lymphomas. In response to H. pylori, interleukin-8 (IL-8) is secreted from host cells to attract components of the innate and adaptive ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.3.1523-1531.2005

    authors: Torok AM,Bouton AH,Goldberg JB

    更新日期:2005-03-01 00:00:00

  • MtsABC is important for manganese and iron transport, oxidative stress resistance, and virulence of Streptococcus pyogenes.

    abstract::MtsABC is a Streptococcus pyogenes ABC transporter which was previously shown to be involved in iron and zinc accumulation. In this study, we showed that an mtsABC mutant has impaired growth, particularly in a metal-depleted medium and an aerobic environment. In metal-depleted medium, growth was restored by the additi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.5.2656-2664.2003

    authors: Janulczyk R,Ricci S,Björck L

    更新日期:2003-05-01 00:00:00

  • Toll-like receptor-2-mediated C-C chemokine receptor 3 and eotaxin-driven eosinophil influx induced by Mycobacterium bovis BCG pleurisy.

    abstract::An acute and persistent eosinophil infiltration is observed during Mycobacterium bovis BCG pleural infection in mice. Eosinophil accumulation, lipid body formation, and eotaxin production were significantly reduced in BCG-infected Toll-like receptor-2 (TLR2)-deficient mice compared to wild-type mice. Neutralization of...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01326-06

    authors: D'Avila H,Almeida PE,Roque NR,Castro-Faria-Neto HC,Bozza PT

    更新日期:2007-03-01 00:00:00

  • Defining the interaction of the Treponema pallidum adhesin Tp0751 with laminin.

    abstract::Various invasive pathogens attach to host tissues via the extracellular matrix component laminin, the major glycoprotein found within basement membranes. Previous investigations identified the laminin-binding adhesin Tp0751 within the spirochete bacterium Treponema pallidum. In the current study, Tp0751 was shown to a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.11.7485-7494.2005

    authors: Cameron CE,Brouwer NL,Tisch LM,Kuroiwa JM

    更新日期:2005-11-01 00:00:00

  • Capsule reduces adherence of enterotoxigenic Escherichia coli to isolated intestinal epithelial cells of pigs.

    abstract::Previous reports have demonstrated that heat-stable (A-type) capsule on piliated enterotoxigenic Escherichia coli enhances colonization of enterotoxigenic E. coli in the small intestine and enhances virulence of enterotoxigenic E. coli. In this report, four encapsulated enterotoxigenic E. coli strains and one encapsul...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.45.3.737-740.1984

    authors: Runnels PL,Moon HW

    更新日期:1984-09-01 00:00:00

  • Klebsiella pneumoniae Expressing VIM-1 Metallo-β-Lactamase Is Resensitized to Cefotaxime via Thiol-Mediated Zinc Chelation.

    abstract::Antibiotic-resistant Klebsiella pneumoniae isolates constitute a great clinical challenge. One important resistance mechanism in K. pneumoniae is the metallo-β-lactamases (MBLs), which require zinc for their function. Thus, zinc chelation could be a strategy to resensitize K. pneumoniae to β-lactams. However, the pote...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00756-19

    authors: Karadottir H,Coorens M,Liu Z,Wang Y,Agerberth B,Giske CG,Bergman P

    更新日期:2019-12-17 00:00:00

  • Effect of dosage on immunogenicity of a Vi conjugate vaccine injected twice into 2- to 5-year-old Vietnamese children.

    abstract::In a double-blind, randomized, and placebo-controlled previous trial, the efficacy of Vi-rEPA for typhoid fever in 2- to 5-year-olds was 89.0% for 46 months. Vi-rEPA contained 25 microg of Vi and induced a greater-than-eightfold rise in immunoglobulin G (IgG) anti-Vi in all of the vaccinees tested. In this investigati...

    journal_title:Infection and immunity

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1128/IAI.72.11.6586-6588.2004

    authors: Canh DG,Lin FY,Thiem VD,Trach DD,Trong ND,Mao ND,Hunt S,Schneerson R,Robbins JB,Chu C,Shiloach J,Bryla DA,Bonnet MC,Schulz D,Szu SC

    更新日期:2004-11-01 00:00:00

  • Effect of iron compounds on antibacterial function of human polymorphs and plasma.

    abstract::Human plasma was bactericidal for small numbers of Klebsiella pneumoniae, but larger numbers grew slowly over a period of 24 h. Human polymorphs in a clot of autologous plasma had a bacteriostatic effect on relatively large numbers of bacteria for up to 24 h and were much more effective than plasma alone. The bacteric...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.51.3.723-730.1986

    authors: Ward CG,Hammond JS,Bullen JJ

    更新日期:1986-03-01 00:00:00

  • Mitogenic activities of synthetic Escherichia coli lipid A and a synthetic partial structure (tripalmitoyl pentapeptide) of E. coli lipoprotein.

    abstract::Synthetic Escherichia coli lipid A and synthetic S-[2,3-bis-(palmitoyloxy)propyl]-N-palmitoylpentapeptide (tripalmitoyl pentapeptide [TPP]), representing the mitogenically active principles of bacterial lipopolysaccharide (LPS) and lipoprotein, respectively, were compared for their mitogenic activities on splenocytes ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.56.5.1382-1384.1988

    authors: Brade L,Bessler WG,Brade H

    更新日期:1988-05-01 00:00:00

  • Regulation of innate immune response to Candida albicans infections by αMβ2-Pra1p interaction.

    abstract::Candida albicans is a common opportunistic fungal pathogen and is the leading cause of invasive fungal diseases in immunocompromised individuals. The induction of cell-mediated immunity to C. albicans is one of the main tasks of cells of the innate immune system, and in vitro evidence suggests that integrin α(M)β₂ (CR...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00650-10

    authors: Soloviev DA,Jawhara S,Fonzi WA

    更新日期:2011-04-01 00:00:00

  • Analysis of the proteinases of representative Trichomonas vaginalis isolates.

    abstract::Isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with acrylamide copolymerized with gelatin (substrate-SDS-PAGE) were combined to evaluate the proteinases of both long-term-grown and fresh isolates of Trichomonas vaginalis. This two-dimensional substrate-SDS-PAGE resolved a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.1.157-162.1990

    authors: Neale KA,Alderete JF

    更新日期:1990-01-01 00:00:00

  • Immunological identity of brucella native hapten, polysaccharide B, and yersinia enterocolitica serotype 9 native hapten.

    abstract::Yersinia enterocolitica serotype 9 contained an antigenic component giving a reaction of total identity with Brucella native hapten and polysaccharide B. This component was present in a phenol-water extract (fraction 5; M. Redfearn, Ph.D. Thesis, University of Wisconsin, Madison, 1960) along with the smooth lipopolysa...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.38.2.778-780.1982

    authors: Fernandez-Lago L,Moriyon I,Toyos J,Diaz R

    更新日期:1982-11-01 00:00:00

  • Rate of Clearance of Vibrio fetus var. venerealis in the Rabbit.

    abstract::The rate of clearance of Vibrio fetus var. venerealis was measured by using in vivo and in vitro methods. The rate of clearance from the circulating blood increased in immune rabbits and reached a plateau 4 days after immunization. Thereafter in vivo clearance did not increase further despite rising agglutinin titers....

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.3.6.815-818.1971

    authors: Carrier SP,Kramer TT

    更新日期:1971-06-01 00:00:00

  • Characterization of Bartonella bacilliformis flagella and effect of antiflagellin antibodies on invasion of human erythrocytes.

    abstract::Bartonella bacilliformis is the etiologic agent of Oroya fever in humans. Flagellum-mediated motility has been postulated as a major virulence factor for invasion of host cells. To address this hypothesis, we purified and characterized flagella from strain KC584 and then assessed their role in human erythrocyte associ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.12.4962-4971.1993

    authors: Scherer DC,DeBuron-Connors I,Minnick MF

    更新日期:1993-12-01 00:00:00

  • Identification of T-cell determinants in natural immune responses to the Plasmodium falciparum apical membrane antigen (AMA-1) in an adult population exposed to malaria.

    abstract::AMA-1 of Plasmodium falciparum is a promising candidate antigen in malaria vaccine development. In this study, we have mapped the immunodominant T-cell determinants in this antigen by using synthetic peptides. From the amphipathic scores, 17 putative T-cell determinants were identified. Nine of the 17 peptides complem...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.64.3.1054-1059.1996

    authors: Lal AA,Hughes MA,Oliveira DA,Nelson C,Bloland PB,Oloo AJ,Hawley WE,Hightower AW,Nahlen BL,Udhayakumar V

    更新日期:1996-03-01 00:00:00

  • Role of neutrophil leukocytes in cutaneous infection caused by Staphylococcus aureus.

    abstract::Despite the high prevalence of cutaneous infections, little is known about the role of host immune responsiveness during Staphylococcus aureus dermatitis. We have recently described a murine model of infectious dermatitis induced by superantigen-producing S. aureus. To assess the role of neutrophils in staphylococcal ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.11.6162-6167.2000

    authors: Mölne L,Verdrengh M,Tarkowski A

    更新日期:2000-11-01 00:00:00

  • Fap2 of Fusobacterium nucleatum is a galactose-inhibitable adhesin involved in coaggregation, cell adhesion, and preterm birth.

    abstract::Fusobacterium nucleatum is a common oral anaerobe involved in periodontitis that is known to translocate and cause intrauterine infections. In the oral environment, F. nucleatum adheres to a large diversity of species, facilitating their colonization and creating biological bridges that stabilize the multispecies dent...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.02838-14

    authors: Coppenhagen-Glazer S,Sol A,Abed J,Naor R,Zhang X,Han YW,Bachrach G

    更新日期:2015-03-01 00:00:00

  • Cutaneous cryptococcosis in athymic and beige-athymic mice.

    abstract::The dermotropism of Cryptococcus neoformans SLHA in congenitally athymic (nu/nu) and doubly immunodeficient beige-athymic (bg/bg-nu/nu) mice is described. Both bg/bg-nu/nu and nu/nu mice developed cutaneous cryptococcosis within 7 to 12 days following intravenous challenge with 10(4) encapsulated yeast cells. Macrosco...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.5.1785-1789.1991

    authors: Salkowski CA,Balish E

    更新日期:1991-05-01 00:00:00

  • In vitro transformation of mouse bone-marrow-derived (B) lymphocytes induced by the lipid component of endotoxin.

    abstract::An analysis of which component of lipopolysaccharide, the lipid or the polysaccharide, is mitogenic for mouse B-lymphocytes has been performed. A purified glycolipid derived from a rough mutant of Salmonella minnesota (R595) that does not contain any o-polysaccharide at all is more mitogenic than an intact lipopolysac...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.8.3.406-411.1973

    authors: Rosenstreich DL,Nowotny A,Chused T,Mergenhagen SE

    更新日期:1973-09-01 00:00:00

  • Antibody recognition and isoelectrofocusing of antigens of the malaria parasite Plasmodium yoelii.

    abstract::Inbred BALB/c mice were either immunized with Triton X-100-extracted antigens of blood-stage Plasmodium yoelii or infected with P. yoelii and cured in three successive schedules. Whereas the immunized BALB/c became only partially protected from subsequent challenge infection with blood-stage P. yoelii, the convalescen...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.11.3909-3916.1991

    authors: Kironde FA,Kumar A,Nayak AR,Kraikov JL

    更新日期:1991-11-01 00:00:00

  • Antibodies to the N-terminal block 2 of Plasmodium falciparum merozoite surface protein 1 are associated with protection against clinical malaria.

    abstract::This longitudinal prospective study shows that antibodies to the N-terminal block 2 region of the Plasmodium falciparum merozoite surface protein 1 (MSP-1) are associated with protection against clinical malaria in an area of stable but seasonal malaria transmission of Ghana. Antibodies to the block 2 region of MSP-1 ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.72.11.6492-6502.2004

    authors: Cavanagh DR,Dodoo D,Hviid L,Kurtzhals JA,Theander TG,Akanmori BD,Polley S,Conway DJ,Koram K,McBride JS

    更新日期:2004-11-01 00:00:00

  • The Legionella pneumophila major secretory protein, a protease, is not required for intracellular growth or cell killing.

    abstract::The Legionella pneumophila major secretory protein (Msp) is a Zn2+ metalloprotease whose function in pathogenesis is unknown. The structural gene for the Msp protease, mspA, was isolated from an L. pneumophila genomic library. In Escherichia coli which contain plasmids with the mspA gene, Msp protein and activity are ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.8.2585-2592.1990

    authors: Szeto L,Shuman HA

    更新日期:1990-08-01 00:00:00

  • Immunogenicity of liposomes and iscoms containing the major outer membrane protein of Neisseria gonorrhoeae: influence of protein content and liposomal bilayer composition.

    abstract::The influences of Neisseria gonorrhoeae protein IB content and bilayer composition of liposomes and protein content of iscoms on immunogenicity were investigated. Changes in the protein content of liposomes did not influence the immunoglobulin G response, whereas the response was lowered when the amount of protein in ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.56.6.1661-1664.1988

    authors: Kersten GF,van de Put AM,Teerlink T,Beuvery EC,Crommelin DJ

    更新日期:1988-06-01 00:00:00