Abstract:
:Gamma interferon (IFN-gamma), a pleiotropic cytokine, is now known to be produced by macrophages as well as by NK cells, gammadelta cells, and activated T cells. The autocrine biological functions of IFN-gamma on the macrophage include the upregulation of major histocompatibility complex MHC class II and the activation to an antiviral state. In this study, the production of IFN-gamma by macrophages was demonstrated to correspond to antibacterial activity. Legionella pneumophila replicates intracellularly in thioglycolate (TG)-elicited macrophages (TG-macrophages) from A/J mice, while TG-macrophages from BALB/c mice restrict bacterial growth after an initial period of growth. BALB/c TG-macrophages were shown to express IFN-gamma mRNA at 24 and 28 h, which corresponded to the initiation of anti-L. pneumophila activity. Moreover, IFN-gammaneutralization by antibody treatment of the cultures resulted in increased L. pneumophila growth in the macrophages. In contrast, no IFN-gamma mRNA was expressed in TG-macrophages from A/J mice, where L. pneumophila grew unrestricted. As would be expected, IFN-gamma treatment decreased bacterial growth. An IFN-gamma-mediated antibacterial activity was, however, inducible in A/J macrophages by the addition of interleukin-12 following L. pneumophila infection. Thus, autocrine IFN-gamma is involved in anti-L. pneumophila activity associated with different growth patterns and appears to be important during intracellular infection.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Salins S,Newton C,Widen R,Klein TW,Friedman Hdoi
10.1128/IAI.69.6.3605-3610.2001keywords:
subject
Has Abstractpub_date
2001-06-01 00:00:00pages
3605-10issue
6eissn
0019-9567issn
1098-5522journal_volume
69pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.130-135.1978
更新日期:1978-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1983-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1978-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1974-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1996-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1986-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1999-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-11-01 00:00:00
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