Abstract:
:Haemophilus influenzae type b infection of immersed and air interface organ cultures for 24 h caused significant epithelial damage. Bacterial association with mucus, damaged epithelium, and unciliated cells was significantly higher in air interface than immersed organ cultures, and total bacterial association was 55.8 times greater. Bacteria exhibited tropism for mucus only on explants maintained with an air interface. We conclude that immersion of nasopharyngeal tissue in medium may influence bacterial interaction with the mucosal surface.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Jackson AD,Cole PJ,Wilson Rdoi
10.1128/IAI.64.6.2353-2355.1996subject
Has Abstractpub_date
1996-06-01 00:00:00pages
2353-5issue
6eissn
0019-9567issn
1098-5522journal_volume
64pub_type
杂志文章abstract::Acute pyelonephritis, a complication of Escherichia coli bacteriuria, must represent a bacterial invasion through the kidney epithelium. To study this process, we overlaid bacterial suspensions onto monolayers of cultured human kidney proximal tubular epithelial cells and measured cytotoxicity by release of lactate de...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.5.1281-1289.1990
更新日期:1990-05-01 00:00:00
abstract::Interferon regulatory factor 1-deficient (IRF-1(-/-)) mice infected with virulent Brucella abortus 2308 at 5 x 10(5) CFU developed acute hepatitis similar to many natural hosts but, unlike natural hosts, IRF-1(-/-) mice were unable to resolve infection and died. In contrast, IRF-1(-/-) mice survived when infected at 5...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.12.7004-7012.2002
更新日期:2002-12-01 00:00:00
abstract::Subcutaneous infection chambers in rabbits were infected with a strain of Staphylococcus aureus isolated from a patient with toxic shock syndrome. Estrogens (mestranol and 17-beta-estradiol) protected male rabbits and prolonged survival. Neither androgens (testosterone and dihydrotestosterone) nor progesterone affecte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.331-333.1986
更新日期:1986-04-01 00:00:00
abstract::It has been postulated that systemic cell-mediated immunity (CMI) is an important host defense mechanism against Candida infections of the vagina. However, in an estrogen-dependent murine model of experimental vaginal candidiasis, we recently showed that systemic Candida-specific Th1-type CMI induced by immunization w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.2.547-553.1995
更新日期:1995-02-01 00:00:00
abstract::Macrophages are among the most sensitive targets of bacterial endotoxin (LPS), responding to minute amounts of LPS by releasing a battery of inflammatory mediators. Transfection of macrophages with secretory leukocyte protease inhibitor (SLPI) renders these cells refractory to LPS stimulation. Here we show that uptake...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4485-4489.1999
更新日期:1999-09-01 00:00:00
abstract::Changes in the structural components of the Streptococcus pyogenes membrane between exponential and early stationary phases of growth are reported. The overall protein composition ranged from 70 to 73% of the dry weight of the membranes, irrespective of the phase of growth from which they were isolated. Amino acid ana...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.883-891.1979
更新日期:1979-12-01 00:00:00
abstract::The adherence of Treponema denticola GM-1, TD-4, and MS25 to human gingival fibroblasts (HGFs) was studied to serve as an introduction to investigations into the interactions of these oral bacteria with human host cells. Under both aerobic (5% CO2) and anaerobic (85% N2 plus 10% H2 plus 5% CO2) environments, the inter...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1720-1729.1990
更新日期:1990-06-01 00:00:00
abstract::We investigated the role of bacterial adherence and hemolysin production from Escherichia coli parent and genetically cloned strains as to their effects on histamine release from rat mast cells and leukotriene generation from human polymorphonuclear granulocytes. These mediators were involved in the induction of infla...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.1.271-278.1985
更新日期:1985-10-01 00:00:00
abstract::Sterile sonic-treated materials of pneumococci grown in media containing N-acetylmannosamine as the carbohydrate source were lethal for mice. Purification of the toxic factor led to its identification as the enzyme neuraminidase. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.1.115-117.1970
更新日期:1970-07-01 00:00:00
abstract::Escherichia coli O157:H7 causes food and waterborne enteric infections that can result in hemorrhagic colitis and life-threatening hemolytic uremic syndrome. Intimate adherence of the bacteria to intestinal epithelial cells is mediated by intimin, but E. coli O157:H7 also possess several other putative adhesins, inclu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05945-11
更新日期:2012-03-01 00:00:00
abstract::A novel in vivo expression technology (IVET) was performed to identify Klebsiella pneumoniae CG43 genes that are specifically expressed during infection of BALB/c mice. The IVET employed a UDP glucose pyrophosphorylase (galU)-deficient mutant of K. pneumoniae which is incapable of utilizing galactose and synthesizing ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.11.7140-7145.2001
更新日期:2001-11-01 00:00:00
abstract::Infection with Giardia duodenalis is one of the most common causes of diarrheal disease in the world. While numerous studies have identified important contributions of adaptive immune responses to parasite control, much less work has examined innate immunity and its connections to the adaptive response during this inf...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00074-16
更新日期:2016-03-24 00:00:00
abstract::The gastric spirillum Helicobacter felis, originally isolated from the cat stomach, colonizes the stomachs of germfree rats. Studies were designed to examine the pathological and serological responses of germfree rats inoculated orally with H. felis. At 2 weeks postinoculation, the gastric mucosa of germfree rats had ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.785-791.1991
更新日期:1991-03-01 00:00:00
abstract::A number of studies have substantiated the pivotal role of innate defense mechanisms in protection against invasive aspergillosis. However, experiments demonstrating increased resistance to lethal intravenous (i.v.) infection with Aspergillus fumigatus conidia in cortisone-treated or untreated mice preinfected with a ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3791-3802.1993
更新日期:1993-09-01 00:00:00
abstract::Helicobacter pylori colonizes the gastric mucosa and secretes a pore-forming toxin (VacA). Two main types of VacA, m1 and m2, can be distinguished by phylogenetic analysis. Type m1 forms of VacA have been extensively studied, but there has been relatively little study of m2 forms. In this study, we generated H. pylori...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00032-20
更新日期:2020-05-20 00:00:00
abstract::The target organs of infection in guinea pigs with asymptomatic acquired or congenital syphilis were identified by PCR and in some cases by rabbit infectivity test (RIT). The prevalence of Treponema pallidum DNA was examined in the following seven organs: the inguinal and mesenteric lymph nodes, spleen, liver, kidney,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3174-3179.1996
更新日期:1996-08-01 00:00:00
abstract::The virulence of Neisseria gonorrhoeae for chicken embryos can be modified in a predictable manner by the addition of microbial siderophores to the inoculum. "Meningobactin" and "gonobactin," siderophores isolated from iron-limited cultures of meningococci and gonococci, respectively, enhance the virulence of the rela...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.2.609-613.1981
更新日期:1981-05-01 00:00:00
abstract::Bovine tumor necrosis factor alpha (TNF-alpha) and interleukin-1 beta (IL-1 beta) cDNAs were generated by reverse transcription and then by PCR amplification from lipopolysaccharide (LPS)-stimulated alveolar macrophage RNA. The amplified cDNAs were cloned into pPow and expressed in Escherichia coli DH5 alpha. The expr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.2.381-388.1995
更新日期:1995-02-01 00:00:00
abstract::Extracytoplasmic proteins were released from Serpulina (Treponema) hyodysenteriae (strain B204) by treatment of whole cells with a nonionic detergent (Tween 20). Centrifugation of the Tween 20-released proteins at 100,000 x g sedimented 10 major extracytoplasmic proteins with approximate molecular masses of 44, 43.5, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.1.142-148.1995
更新日期:1995-01-01 00:00:00
abstract::Host resistance to African trypanosomiasis is partially dependent on an early and strong T-independent B-cell response against the variant surface glycoprotein (VSG) coat expressed by trypanosomes. The repetitive array of surface epitopes displayed by a monotypic surface coat, in which identical VSG molecules are clos...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.5.2690-2697.2005
更新日期:2005-05-01 00:00:00
abstract::In this study, the role of the hepatobiliary system in the early pathogenesis of Salmonella enteritidis infection was investigated in a rat model. Intravenous (i.v.) challenge with lipopolysaccharide (LPS) has previously been shown to enhance the translocation of normal gut flora. We first confirmed that LPS can simil...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.1.1-5.2000
更新日期:2000-01-01 00:00:00
abstract::Zoonotic infections caused by Salmonella enterica serovar Typhimurium pose a constant threat to consumer health, with the pig being a particularly major source of multidrug-resistant isolates. Vaccination, as a promising approach to reduce colonization and shedding, has been scarcely used, as it interferes with curren...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01908-06
更新日期:2007-05-01 00:00:00
abstract::Clinical trials of novel tuberculosis (TB) vaccines are expensive, while global resources for TB vaccine development are limited. Therefore, there is a need for robust and predictive preclinical data to support advancement of candidate vaccines into clinical trials. Here, we provide a rationale for using the nonhuman ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00776-17
更新日期:2018-01-22 00:00:00
abstract::Tuberculosis (TB) is a leading cause worldwide of human mortality attributable to a single infectious agent. Recent studies targeting candidate genes and "case-control" association have revealed numerous polymorphisms implicated in host susceptibility to TB. Here, we review current progress in the understanding of cau...
journal_title:Infection and immunity
pub_type: 杂志文章,评审
doi:10.1128/IAI.00443-12
更新日期:2012-10-01 00:00:00
abstract::The mechanisms that govern the initial interaction between Paracoccidioides brasiliensis, a primary dimorphic fungal pathogen, and cells of the innate immunity need to be clarified. Our previous studies showed that Toll-like receptor 2 (TLR2) and TLR4 regulate the initial interaction of fungal cells with macrophages a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00375-10
更新日期:2011-06-01 00:00:00
abstract::Escherichia coli F-18, isolated from the feces of a healthy human in 1977, is an excellent colonizer of the streptomycin-treated mouse large intestine and displays normal motility and chemotaxis ability. A chemotaxis-defective derivative of E. coli F-18, E, coli F-18 CheA-, and a nonflagellated derivative, E. coli F-1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.2957-2961.1990
更新日期:1990-09-01 00:00:00
abstract::The HT29MTXE12 (E12) cell line harbors an adherent mucus layer, providing a novel technique to model mucosal infection in vitro. In this study, we have characterized the interaction of Campylobacter jejuni with the E12 cell line and exploited its unique mucus layer to examine the potential efficacy of probiotic treatm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01249-09
更新日期:2010-06-01 00:00:00
abstract::Mycobacterium leprae are killed by myeloperoxidase (or eosinophil peroxidase), H2O2, and a halide, thus suggesting a mechanism for their destruction by peroxidase-containing phagocytes. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.2.534-536.1984
更新日期:1984-05-01 00:00:00
abstract::In this study, the efficacy of bactericidal/permeability-increasing protein (BPI) was assessed in a rat model of chronic otitis media with effusion. BPI injection prevented disturbance of the mucociliary clearance system of the middle ear. Hence, it is postulated that BPI can be a new therapy for chronic otitis media ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2992-2994.2000
更新日期:2000-05-01 00:00:00
abstract::A gene, dmcA, expressing a methyl-accepting chemotaxis protein (MCP) from the oral spirochete Treponema denticola has been characterized. The gene was initially identified as an open reading frame immediately upstream from the previously characterized prtB protease gene of strain ATCC 35405. Nucleotide sequencing of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4011-4016.1997
更新日期:1997-10-01 00:00:00