Abstract:
:Extracytoplasmic proteins were released from Serpulina (Treponema) hyodysenteriae (strain B204) by treatment of whole cells with a nonionic detergent (Tween 20). Centrifugation of the Tween 20-released proteins at 100,000 x g sedimented 10 major extracytoplasmic proteins with approximate molecular masses of 44, 43.5, 42, 39, 38, 34, 33.5, 33, 31, and 29 kDa. Treatment of the sedimented fraction with 6 M urea solubilized all of the proteins except the 39-kDa protein. Peptide sequences were obtained for the purified 42-, 39-, 38-, 34-, 31-, and 29-kDa proteins. The peptide sequences of the 42-, 38-, and 31-kDa proteins indicate that they likely are components of the periplasmic flagella. The amino-terminal peptide sequence of the 38-kDa protein was used to design an oligonucleotide probe and to clone an S. hyodysenteriae DNA fragment containing the gene encoding this protein. The predicted 290-amino-acid protein sequence derived from the cloned gene was highly homologous to those of several other bacterial flagellar proteins and is preceded by consensus sigma D nucleotide sequences found upstream of other flagellar genes. On the basis of its similarity to the FlaB proteins of other spirochetes, we propose to designate the cloned S. hyodysenteriae gene flaB1 and its encoded protein FlaB1. Vaccination of pigs with FlaB1 or its recombinant counterpart did not protect them from an experimental challenge.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Gabe JD,Chang RJ,Slomiany R,Andrews WH,McCaman MTdoi
10.1128/IAI.63.1.142-148.1995subject
Has Abstractpub_date
1995-01-01 00:00:00pages
142-8issue
1eissn
0019-9567issn
1098-5522journal_volume
63pub_type
杂志文章abstract::Vibrio cholerae responds to environmental changes by altering the protein composition of its outer membrane. In rich medium, V. cholerae expresses almost exclusively the outer membrane porin OmpU, whereas in minimal medium, OmpT is the dominant porin. The supplementation of a minimal medium with a mixture of asparagin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05851-11
更新日期:2012-02-01 00:00:00
abstract::The target organs of infection in guinea pigs with asymptomatic acquired or congenital syphilis were identified by PCR and in some cases by rabbit infectivity test (RIT). The prevalence of Treponema pallidum DNA was examined in the following seven organs: the inguinal and mesenteric lymph nodes, spleen, liver, kidney,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3174-3179.1996
更新日期:1996-08-01 00:00:00
abstract::Most oral bacteria tested formed colonies on a chemically defined medium with a chromogenic arylsulfatase substrate. Arylsulfatase activity was, however, restricted to Campylobacter-Wolinella group organisms, including Wolinella recta, a possible periodontopathogen. W. recta was the only arylsulfatase-active species a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1380-1383.1989
更新日期:1989-05-01 00:00:00
abstract::The blood-stage development of malaria parasites is initiated by the invasion of merozoites into susceptible erythrocytes. Specific receptor-ligand interactions must occur for the merozoites to first attach to and then invade erythrocytes. Because the invasion process is essential for the parasite's survival and the m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.12.5581-5586.1994
更新日期:1994-12-01 00:00:00
abstract::Several trivalent cations, including lanthanum (La3+), inhibited the secretion (enterosorption) induced by the enterotoxins of Vibrio cholerae and Escherichia coli in the rabbit ileum in vivo. High concentrations (greater than 10 mM) of La3+ were required to inhibit cholera enterotoxin (CE)-induced enterosorption, pro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.5.1038-1044.1975
更新日期:1975-05-01 00:00:00
abstract::We have constructed an improved recombination-based in vivo expression technology (RIVET) and used it as a screening method to identify Vibrio cholerae genes that are transcriptionally induced during infection of infant mice. The improvements include the introduction of modified substrate cassettes for resolvase that ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.2.972-980.2005
更新日期:2005-02-01 00:00:00
abstract::Brucella abortus is recognized by several Toll-like receptor (TLR)-associated pathways triggering proinflammatory responses that affect both the nature and intensity of the immune response. Previously, we demonstrated that B. abortus-mediated dendritic cell (DC) maturation and control of infection are dependent on the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01356-12
更新日期:2013-05-01 00:00:00
abstract::Athymic BALB/c nude mice and euthymic BALB/c mice were infected with Rickettsia akari by the intraperitoneal route. The rickettsialpox infection was terminated in euthymic mice with only two intraperitoneal injections of the antibiotic oxytetracycline, whereas prolonged treatment was necessary to terminate the infecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::It is proposed that binding of oral streptococci to saliva-coated hydroxylapatite (SHA) surfaces is a multifactorial process involving both specific and nonspecific receptors. In this context, specific binding is described as a high-affinity, saturable interaction between the cell and binding surface. Conversely, nons...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.3.653-659.1984
更新日期:1984-06-01 00:00:00
abstract::The goal of this study is to evaluate the contribution of mast cells to Helicobacter pylori immunity in a model of vaccine-induced protection. Mast cell-deficient Kitl(Sl)/Kitl(Sl-d) and control mice were immunized with H. pylori sonicate plus cholera toxin and challenged with H. pylori, and the bacterial loads, infla...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00532-09
更新日期:2009-12-01 00:00:00
abstract::The antigenic relationship of glucosyltransferases (GTF) produced by different serotypes of Streptococcus mutans was studied by using a functional inhibition assay. Rat, rabbit, or hamster immune fluids, directed to cell-associated or supernatant-derived GTF, were tested against ammonium sulfate-precipitated culture s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.91-103.1977
更新日期:1977-01-01 00:00:00
abstract::The vast majority of cases of gram-negative meningitis in neonates are caused by K1-encapsulated Escherichia coli. The role of the K1 capsule in the pathogenesis of E. coli meningitis was examined with an in vivo model of experimental hematogenous E. coli K1 meningitis and an in vitro model of the blood-brain barrier....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3566-3570.1999
更新日期:1999-07-01 00:00:00
abstract::Specific proteolytic destruction of the human chemotaxin, C5a, is a property of group A and B streptococcal pathogens. Here we show that virulent group G streptococci from human sources also express C5a peptidase activity. The enzyme responsible for this activity is approximately the same size as and is antigenically ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.7.2305-2310.1991
更新日期:1991-07-01 00:00:00
abstract::Stools from a patient with antibiotic-associated colitis and cecal contents from a hamster with clindamycin-induced enterocolitis were compared in a cytotoxicity assay to determine common properties. Both specimens produced actinomorphic changes in human amnion cells at 10(-7) dilutions. The toxin was acid labile, hea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.2.526-529.1978
更新日期:1978-05-01 00:00:00
abstract::Staphylococcus aureus can produce a wide variety of exotoxins, including toxic shock syndrome toxin 1 (TSST-1), staphylococcal enterotoxins, and staphylococcal enterotoxin-like toxins. These toxins share superantigenic activity. To investigate the beta chain (Vbeta) specificities of each of these toxins, TSST-1 and al...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01388-08
更新日期:2009-05-01 00:00:00
abstract::Mouse fibroblasts (L cells) were infected in suspension with Chlamydia psittaci (6BC) and then plated out on a solid substrate at a density of 80 cells per cm2 so that the effect of chlamydial infection on the division of single host cells and their progeny could be determined. Uninfected L cells multiplied with a mea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.1.281-286.1978
更新日期:1978-01-01 00:00:00
abstract::Mycoplasma arthritidis causes acute polyarthritis in rats and chronic proliferative arthritis in mice. M. arthritidis-induced arthritis serves as a model for arthritis caused by infectious agents and as a model for examining the role of the superantigen MAM (M. arthritidis T-cell mitogen) in the development of autoimm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.245-249.2005
更新日期:2005-01-01 00:00:00
abstract::Host-driven macrophage apoptosis contributes to innate immunity during bacterial infection. Neisseria meningitidis inhibits apoptosis in a variety of cells, but its impact on macrophage apoptosis is unknown. We demonstrate that N. meningitidis prevents macrophage apoptosis via genes encoding nitric oxide detoxificatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.1.729-733.2006
更新日期:2006-01-01 00:00:00
abstract::Although Salmonella enterica serovar Typhimurium can undergo phase variation to alternately express two different types of flagellin subunit proteins, FljB or FliC, no biological function for this phenomenon has been described. In this investigation, we constructed phase-locked derivatives of S. enterica serovar Typhi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3021-3030.2001
更新日期:2001-05-01 00:00:00
abstract::A murine monoclonal antibody was raised against the B95-8 strain of Epstein-Barr virus (EBV), which was isolated from a case of mononucleosis after blood transfusion (Hoffman et al. Proc. Natl. Acad. Sci. U.S.A. 77:2979, 1980). We provide evidence that neutralization of immortalization by this monoclonal antibody is v...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.3.1028-1031.1982
更新日期:1982-09-01 00:00:00
abstract::We performed ex vivo experiments with Plasmodium falciparum-infected human placentas from primi- and multigravida women from Cameroon. All women, independent of their gravida status, had anti-chondroitin sulfate A (CSA) adhesion antibodies which cross-reacted with heterologous strains, such as FCR3 and Palo-Alto(FUP)1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Using a murine respiratory infection model, we have demonstrated previously that infection with Bordetella pertussis or immunization with a whole-cell pertussis vaccine induced antigen-specific Th1 cells, which conferred a high level of protection against aerosol challenge. In contrast, immunization with an acellular ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5295-5301.1996
更新日期:1996-12-01 00:00:00
abstract::Bovine sera for tissue culture use were shown to contain heterophil antigen of the Paul-Bunnell-Davidsohn type by immune adherence hemagglutination tests with sera from patients with infectious mononucleosis. The antibody titers observed were comparable to those determined by two other methods; i.e., the immune adhere...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.923-927.1978
更新日期:1978-03-01 00:00:00
abstract::The epitope specificities of 13 hybridomas secreting monoclonal antibodies (MAbs) specific for pertussis toxin (PT) is described. Hybridoma lines were derived by the fusion of spleen cells from mice immunized with native PT, Formalin-detoxified PT, or isolated PT subunits (S1 to S5) with the myeloma line X63-Ag8.653. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.9.2660-2665.1989
更新日期:1989-09-01 00:00:00
abstract::Mucins are high-molecular-weight glycoproteins and major constituents of the mucus layer which covers the airway surface. We have studied the interactions between bacteria, mucins, and epithelial cells from the human respiratory tract. Nontypeable strains of Haemophilus influenzae were found to bind to purified airway...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2485-2492.1995
更新日期:1995-07-01 00:00:00
abstract::Anopheles gambiae is the major African vector of Plasmodium falciparum, the most deadly species of human malaria parasite and the most prevalent in Africa. Several strategies are being developed to limit the global impact of malaria via reducing transmission rates, among which are transmission-blocking vaccines (TBVs)...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00864-06
更新日期:2007-04-01 00:00:00
abstract::To evaluate the roles of the infectious agent and the host in the pathogensis of Salmonella-associated arthritis (SAA), 1O(3) to 10(6) heat-killed Salmonella enteritidis were injected directly into involved carpal joints in normal and actively immunized rats and in hosts adoptively immunized by the intravenous transfe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.4.1155-1160.1976
更新日期:1976-04-01 00:00:00
abstract::The ability to bind and utilize hemin is a trait common to many human pathogens. Nevertheless, the relationship between Legionella pneumophila, the agent of Legionnaires' disease, and hemin has received little attention. Thus, we explored the capacity of a virulent, serogroup 1 strain of L. pneumophila to bind hemin a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.3.842-848.1996
更新日期:1996-03-01 00:00:00
abstract::The antigens of Haemophilus somnus recognized by convalescent bovine serum were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting with a protein A-peroxidase conjugate. The same two 76K and 40K antigens were predominant in whole-bacterium preparations and in outer-membrane-enric...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.6.1381-1386.1987
更新日期:1987-06-01 00:00:00
abstract::Production of group A streptococcal pyrogenic exotoxins (SPE) type A and C was transferred from toxigenic streptococcal strains to nontoxigenic strains by lysogeny. Lysogens were tested for SPE with Ouchterlony immunodiffusion on Todd-Hewitt agar plates; toxin diffusing from isolated colonies reacted with specific hyp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.28.1.254-257.1980
更新日期:1980-04-01 00:00:00