Abstract:
:The goal of this study is to evaluate the contribution of mast cells to Helicobacter pylori immunity in a model of vaccine-induced protection. Mast cell-deficient Kitl(Sl)/Kitl(Sl-d) and control mice were immunized with H. pylori sonicate plus cholera toxin and challenged with H. pylori, and the bacterial loads, inflammatory infiltrates, and cytokine responses were evaluated and compared at 1, 2, and 4 weeks postchallenge. In vitro stimulation assays were performed using bone marrow-derived mast cells, and recall assays were performed with spleen cells of immunized mast cell-deficient and wild-type mice. Bacterial clearance was observed by 2 weeks postchallenge in mast cell-deficient mice. The bacterial load was reduced by 4.0 log CFU in wild-type mice and by 1.5 log CFU in mast cell-deficient mice. Neutrophil numbers in the gastric mucosa of immune Kitl(Sl)/Kitl(Sl-d) mice were lower than those for immune wild-type mice (P < 0.05). Levels of gastric interleukin-17 (IL-17) and tumor necrosis factor alpha (TNF-alpha) were also significantly lower in immune Kitl(Sl)/Kitl(Sl-d) mice than in wild-type mice (P < 0.001). Immunized mast cell-deficient and wild-type mouse spleen cells produced IFN-gamma and IL-17 in response to H. pylori antigen stimulation. TNF-alpha and CXC chemokines were detected in mast cell supernatants after 24 h of stimulation with H. pylori antigen. The results indicate that mast cells are not essential for but do contribute to vaccine-induced immunity and that mast cells contribute to neutrophil recruitment and inflammation in response to H. pylori.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Ding H,Nedrud JG,Wershil B,Redline RW,Blanchard TG,Czinn SJdoi
10.1128/IAI.00532-09subject
Has Abstractpub_date
2009-12-01 00:00:00pages
5543-50issue
12eissn
0019-9567issn
1098-5522pii
IAI.00532-09journal_volume
77pub_type
杂志文章abstract::This study assessed the effect of periodontal therapy on specific serum antibody concentration, expressed as titer, and antibody binding strength, expressed as relative avidity. The immune responses to Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans were investigated. Antibody titer was assayed by en...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.9.3411-3416.1995
更新日期:1995-09-01 00:00:00
abstract::Type II heat-labile enterotoxin (LT-II) from Escherichia coli 41 was purified and compared with prototype LT-II encoded by genes from E. coli SA53. Both toxins were oligomeric proteins consisting of polypeptides A (Mr, 28,000) and B (Mr, 11,800). The A polypeptides were cleaved by trypsin into fragments A1 (Mr, 21,000...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.2.529-536.1986
更新日期:1986-11-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.65.5.1962-1966.1997
更新日期:1997-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.12.7153-7155.2002
更新日期:2002-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.3.798-803.1980
更新日期:1980-03-01 00:00:00
abstract::Endotoxin may cause abnormal deposition of platelet-endotoxin aggregates, and this event could have damaging effects. We compared the aggregation characteristics of platelets from rabbits made tolerant to the lethal effects of endotoxin with those of platelets from normal rabbits. Platelets from tolerant rabbits aggre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.919-922.1978
更新日期:1978-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.1126-1135.1983
更新日期:1983-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.9.4877-4883.2000
更新日期:2000-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.667-676.1976
更新日期:1976-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.1.124-131.1977
更新日期:1977-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3823-3826.1991
更新日期:1991-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.3.1606-1612.2005
更新日期:2005-03-01 00:00:00
abstract::Performing patch-clamp experiments on human macrophages, we show that the K(+) channel MaxiK is activated by lipopolysaccharide, peptidoglycan, and interleukin-1. Cytokine production initiated by several Toll-like receptor (TLR) ligands and by interleukin-1 is inhibited by MaxiK blockade. This provides evidence for fu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01783-05
更新日期:2006-07-01 00:00:00
abstract::HbhA of Mycobacterium tuberculosis is a multifunctional binding protein, binding to both sulfated sugars such as heparin and to human complement component C3. HbhA may therefore interact with host molecules and/or host cells during M. tuberculosis infection and play a role in the pathogenesis of this bacterium. The pu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.12.6751-6760.2002
更新日期:2002-12-01 00:00:00
abstract::In previous studies, we reported that fibronectin (FN) markedly enhances phagocytic uptake of antibody-coated group B streptococci (GBS) by human polymorphonuclear leukocytes. Furthermore, administration of FN along with a GBS type-specific monoclonal or polyclonal antibody to infected neonatal rats significantly enha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2334-2339.1993
更新日期:1993-06-01 00:00:00
abstract::In the last few years, the great impact of antimicrobial peptides on infectious disease susceptibility and natural resistance has been reported. In some cases, susceptibility to diseases is related to antimicrobial peptide polymorphisms and gene copy numbers, but for the vast majority of infectious diseases, these phe...
journal_title:Infection and immunity
pub_type: 杂志文章,评审
doi:10.1128/IAI.01515-08
更新日期:2009-11-01 00:00:00
abstract::Rats and mice infected with Trypanosoma lewisi and Trypanosoma musculi, respectively, showed hyperreactivity to lipopolysaccharide (LPS) from gram-negative bacteria. Fatal shock could be precipitated with a dose of LPS 100 to 1,000 times less in infected compared with noninfected animals. In trypanosome-infected rats ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.2.501-506.1984
更新日期:1984-11-01 00:00:00
abstract::The comparative immunogenicity of the two cross-reacting group 6 pneumococcal capsular polysaccharides, type 6A(6) and type 6B(26), was studied with hyperimmune rabbit typing antisera and with sera from adult volunteers injected with polyvalent pneumococcal vaccines containing either 50 mug of type 6A (U.S. designatio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.1116-1122.1979
更新日期:1979-12-01 00:00:00
abstract::Individuals in areas of Plasmodium falciparum endemicity develop immunity to malaria after repeated exposure. Knowledge of the acquisition and nature of protective immune responses to P. falciparum is presently limited, particularly for young children. We examined antibodies (IgM, IgG, and IgG subclasses) to merozoite...
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更新日期:2015-02-01 00:00:00
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更新日期:2006-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.5.2096-2100.1992
更新日期:1992-05-01 00:00:00
abstract::Several immunological, hematological, and pathological responses associated with Marek's disease were determined. Four-week-old Marek's disease-infected and control chickens were injected with Salmonella pullorum antigen. About one-half of all infected chickens tested were unresponsive to antigenic challenge. Antibody...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.5.567-574.1971
更新日期:1971-11-01 00:00:00
abstract::The intravascular trematode Schistosoma mansoni is a causative agent of schistosomiasis, a disease that constitutes a major health problem globally. In this study we cloned and characterized the schistosome tegumental phosphodiesterase SmNPP-5 and evaluated its role in parasite virulence. SmNPP-5 is a 52.5-kDa protein...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05431-11
更新日期:2011-10-01 00:00:00
abstract::Adhesion of Trichomonas vaginalis is believed to be dependent on four adhesion proteins, which are thought to bind to vaginal epithelial cells in a specific manner with a ligand-receptor type of interaction. However, the specific receptors on the host cell have not yet been identified. In this work, the ability of the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4358-4360.2000
更新日期:2000-07-01 00:00:00
abstract::In this study, we examined the binding of Candida albicans synchronized yeast-phase cells to plastic, immobilized amino acids and bovine serum albumin (BSA) and quantified the binding by using an XTT tetrazolium salt assay and absorbance determination. Our results show that C. albicans binds efficiently and specifical...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.1.140-144.1998
更新日期:1998-01-01 00:00:00
abstract::The population structure of the Bacillus cereus group (52 strains of B. anthracis, B. cereus, and B. thuringiensis) was investigated by sequencing seven gene fragments (rpoB, gyrB, pycA, mdh, mbl, mutS, and plcR). Most of the strains were classifiable into two large subgroups in six housekeeping gene trees but not in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5253-5261.2004
更新日期:2004-09-01 00:00:00
abstract::The incidence of translocation of viable Escherichia coli C25 from the gastrointestinal tract to the mesenteric lymph nodes was compared in gnotobiotic mice colonized with only E. coli C25 and in gnotobiotic mice colonized with E. coli C25 plus the whole cecal flora from specific pathogen-free mice. The population lev...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.3.820-827.1979
更新日期:1979-09-01 00:00:00
abstract::Specific pathogen-free guinea pigs were infected via the respiratory route with viable, attenuated Mycobacterium tuberculosis H37Ra and maintained on purified isocaloric diets. The control diet contained 30% protein (ovalbumin) and 50 ppm of added zinc (50 micrograms/g), the low protein diet contained 10% protein and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.793-799.1983
更新日期:1983-02-01 00:00:00
abstract::Immunization of mice with Plasmodium berghei or Plasmodium yoelii synthetic linear peptide chimeras (LPCs) based on the circumsporozoite protein protects against experimental challenge with viable sporozoites. The immunogenicity of LPCs is significantly enhanced by spontaneous polymerization. To better understand the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00470-08
更新日期:2009-05-01 00:00:00
abstract::Subtilase cytotoxin (SubAB) is a recently identified AB5 subunit toxin produced by Shiga-toxigenic Escherichia coli. The A subunit is thought to be a subtilase-like, serine protease, whereas the B subunit binds to the toxin receptor on the cell surface. We cloned the genes from a clinical isolate; the toxin was produc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01336-06
更新日期:2007-01-01 00:00:00