Abstract:
:We identified and characterized a gene encoding a homologue of the B subunits of cholera toxin (CTB) and heat-labile enterotoxin (LTB) of Escherichia coli from a clinical isolate of Citrobacter freundii that was found to produce a factor in the culture supernatant that cross-reacted with antibodies to CTB and LTB when assayed by enzyme-linked immunosorbent assay (ELISA). The gene encoding the ELISA-positive factor, cfxB, consisted of 375 nucleotides and was located downstream of an 852-nucleotide open reading frame, cfxA, with a 56-nucleotide intergenic space. The cfxB gene was predicted to encode a 125-amino-acid polypeptide, which had 73.8 and 72.8% identities with the amino acid sequences of LTB and CTB, respectively. However, the amino acid sequence of the deduced polypeptide CFXA had no homologies to those of the A subunits of CT or LT. DNA probes developed from the sequences of cfxA and cfxB were used to screen 67 C. freundii isolates and 152 E. coli isolates from diarrheal patients by colony blot hybridization. Two strains, C. freundii 48 and E. coli 176, reacted with both DNA probes under conditions of high stringency. We cloned homologues of the cfxA and cfxB genes from E. coli 176 and designated them ecxA and ecxB, respectively. The ecxA gene and the ecxB gene comprise 855 and 375 nucleotides, respectively, with a 50-nucleotide intergenic space, and encode a 285- and a 125-amino-acid residue polypeptides, respectively. The results of the present study may provide important clues to the origin and evolution of immunologically related factors sharing a common enterotoxin-like A and B subunit structures.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Karasawa T,Ito H,Tsukamoto T,Yamasaki S,Kurazono H,Faruque SM,Nair GB,Nishibuchi M,Takeda Ydoi
10.1128/iai.70.12.7153-7155.2002keywords:
subject
Has Abstractpub_date
2002-12-01 00:00:00pages
7153-5issue
12eissn
0019-9567issn
1098-5522journal_volume
70pub_type
杂志文章abstract::A radioimmunoprecipitation method has been devised which permits the direct identification of those proteins which are both exposed on the cell surface of Haemophilus influenzae type b and accessible to antibody. Both extrinsically and intrinsically radiolabeled cells of this organism were employed as the sources of a...
journal_title:Infection and immunity
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doi:10.1128/IAI.33.3.950-953.1981
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doi:10.1128/IAI.7.3.398-402.1973
更新日期:1973-03-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.22.2.555-559.1978
更新日期:1978-11-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.46.1.272-275.1984
更新日期:1984-10-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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pub_type: 杂志文章
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journal_title:Infection and immunity
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doi:
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更新日期:1985-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.48.3.676-685.1985
更新日期:1985-06-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1983-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1994-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1975-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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更新日期:1982-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1989-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.11.4147-4153.1991
更新日期:1991-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1988-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1991-11-01 00:00:00