Abstract:
:Di-, tri-, and tetrasaccharides, synthesized according to the chemical structure of pneumococcal polysaccharide type 3 (PS3), were coupled to the cross-reactive material (CRM(197)) of modified diphtheria toxin in different molar carbohydrate/protein ratios using the squarate coupling method. To study protective immunity, female BALB/c mice were subcutaneously immunized twice (with a 3-week interval) using the amount of conjugates corresponding to 2.5 microg of oligosaccharide per mouse. The conjugates evoked PS3 binding immunoglobulin G antibodies that lasted for at least 7 weeks after the booster. Immunogenicity was not influenced by the carbohydrate/protein ratio. All mice with PS3-specific antibodies survived the intraperitoneal challenge with Streptococcus pneumoniae type 3. Therefore, synthetic oligosaccharide-protein conjugates might have potential as vaccines.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Benaissa-Trouw B,Lefeber DJ,Kamerling JP,Vliegenthart JF,Kraaijeveld K,Snippe Hdoi
10.1128/IAI.69.7.4698-4701.2001keywords:
subject
Has Abstractpub_date
2001-07-01 00:00:00pages
4698-701issue
7eissn
0019-9567issn
1098-5522journal_volume
69pub_type
杂志文章abstract::Malaria infection treatment vaccine (ITV) is a promising strategy to induce homologous and heterologous protective immunity against the blood stage of the parasite. However, the underlying mechanism of protection remains largely unknown. Here, we found that a malaria-specific antibody (Ab) could mediate the protective...
journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.25.1.366-375.1979
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doi:10.1128/IAI.02785-14
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journal_title:Infection and immunity
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doi:10.1128/IAI.21.3.879-885.1978
更新日期:1978-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.3.775-779.1977
更新日期:1977-12-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1998-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.5.2096-2102.1999
更新日期:1999-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.276-281.1979
更新日期:1979-04-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1984-09-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1987-08-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1985-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.3.1167-1174.1983
更新日期:1983-03-01 00:00:00
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更新日期:1999-03-01 00:00:00