Abstract:
:Pathogenic Yersinia species utilize a type III secretion system (T3SS) to translocate effectors called Yersinia outer proteins (Yops) into infected host cells. Previous studies demonstrated a role for effector Yops in the inhibition of caspase-1-mediated cell death and secretion of interleukin-1beta (IL-1beta) in naïve macrophages infected with Yersinia enterocolitica. Naïve murine macrophages were infected with a panel of different Yersinia pestis and Yersinia pseudotuberculosis strains to determine whether Yops of these species inhibit caspase-1 activation. Cell death was measured by release of lactate dehydrogenase (LDH), and enzyme-linked immunosorbent assay for secreted IL-1beta was used to measure caspase-1 activation. Surprisingly, isolates derived from the Y. pestis KIM strain (e.g., KIM5) displayed an unusual ability to activate caspase-1 and kill infected macrophages compared to other Y. pestis and Y. pseudotuberculosis strains tested. Secretion of IL-1beta following KIM5 infection was reduced in caspase-1-deficient macrophages compared to wild-type macrophages. However, release of LDH was not reduced in caspase-1-deficient macrophages, indicating that cell death occurred independently of caspase-1. Analysis of KIM-derived strains defective for production of functional effector or translocator Yops indicated that translocation of catalytically active YopJ into macrophages was required for caspase-1 activation and cell death. Release of LDH and secretion of IL-1beta were not reduced when actin polymerization was inhibited in KIM5-infected macrophages, indicating that extracellular bacteria translocating YopJ could trigger cell death and caspase-1 activation. This study uncovered a novel role for YopJ in the activation of caspase-1 in macrophages.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Lilo S,Zheng Y,Bliska JBdoi
10.1128/IAI.01695-07subject
Has Abstractpub_date
2008-09-01 00:00:00pages
3911-23issue
9eissn
0019-9567issn
1098-5522pii
IAI.01695-07journal_volume
76pub_type
杂志文章abstract::In vitro experimentation indicates that periodontitis-associated bacteria contain potent polyclonal B-cell activators (PBA). We reasoned that if PBA were operative in vivo, plasma cells specific for nonoral antigens should be present in the inflamed gingival tissues, which are characterized by a plasma cell infiltrate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.823-830.1988
更新日期:1988-04-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.00711-15
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.1.52-60.1985
更新日期:1985-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3488-3493.1999
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.1.116-121.1997
更新日期:1997-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4793-4800.1993
更新日期:1993-11-01 00:00:00
abstract::Schistosoma mansoni eggs produced by adult worms in the mesenteric vasculature become trapped in the liver, where they induce granulomatous lesions and strong immune responses. Infected individuals suffer from intestinal schistosomiasis (INT) in 90% of cases, whereas the remaining 10% present with severe hepatosplenic...
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pub_type: 杂志文章
doi:10.1128/IAI.00647-09
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.3.773-777.1984
更新日期:1984-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.4358-4360.2000
更新日期:2000-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.3.646-653.1990
更新日期:1990-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.3.775-778.1981
更新日期:1981-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.1.31-36.1986
更新日期:1986-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3052-3057.1988
更新日期:1988-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.4997-5002.1994
更新日期:1994-11-01 00:00:00
abstract::CS31A is a K88-related capsule-like surface protein that mediates Escherichia coli and Klebsiella pneumoniae adhesion to the human Caco-2 and Intestine-407 cell lines. In this study, we demonstrate that ClpG, the major subunit of CS31A, contains the adhesive domain of the polymerized structure. We mapped this domain w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.609-616.1997
更新日期:1997-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.8.3527-3534.1998
更新日期:1998-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.1.117-120.1977
更新日期:1977-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.236-239.1974
更新日期:1974-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.12.7322-7325.2004
更新日期:2004-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.1.229-237.2004
更新日期:2004-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4698-4701.2001
更新日期:2001-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00287-10
更新日期:2010-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.3.837-840.1986
更新日期:1986-12-01 00:00:00
abstract::Adherence of blastoconidia to epidermal corneocytes is an early event in Candida colonization and infection of the skin. Pathogenic species adhere more avidly than nonpathogenic species, transform to hyphal growth, and invade the stratum corneum of the skin. Adherence was studied by scanning electron microscopy of exp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.1942-1949.1988
更新日期:1988-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.6.1182-1186.1975
更新日期:1975-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1374-1379.1989
更新日期:1989-05-01 00:00:00
abstract::Haemophilus ducreyi previously has been shown to inhibit the phagocytosis of both secondary targets and itself by certain cells in vitro. Wild-type H. ducreyi strain 35000HP contains two genes, lspA1 and lspA2, whose encoded protein products are predicted to be 456 and 543 kDa, respectively. An isogenic mutant of H. d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.10.5994-6003.2003
更新日期:2003-10-01 00:00:00
abstract::Monoclonal antibody WM-25 inhibited the in vitro growth of 13 F38 isolates from goats with contagious caprine pleuropneumonia but not 7 heterologous mycoplasma isolates representing four different species. In contrast to results with polyclonal antisera, growth inhibition by monoclonal antibody WM-25 was specific for ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.12.3219-3220.1987
更新日期:1987-12-01 00:00:00