Caspase-1 activation in macrophages infected with Yersinia pestis KIM requires the type III secretion system effector YopJ.

Abstract:

:Pathogenic Yersinia species utilize a type III secretion system (T3SS) to translocate effectors called Yersinia outer proteins (Yops) into infected host cells. Previous studies demonstrated a role for effector Yops in the inhibition of caspase-1-mediated cell death and secretion of interleukin-1beta (IL-1beta) in naïve macrophages infected with Yersinia enterocolitica. Naïve murine macrophages were infected with a panel of different Yersinia pestis and Yersinia pseudotuberculosis strains to determine whether Yops of these species inhibit caspase-1 activation. Cell death was measured by release of lactate dehydrogenase (LDH), and enzyme-linked immunosorbent assay for secreted IL-1beta was used to measure caspase-1 activation. Surprisingly, isolates derived from the Y. pestis KIM strain (e.g., KIM5) displayed an unusual ability to activate caspase-1 and kill infected macrophages compared to other Y. pestis and Y. pseudotuberculosis strains tested. Secretion of IL-1beta following KIM5 infection was reduced in caspase-1-deficient macrophages compared to wild-type macrophages. However, release of LDH was not reduced in caspase-1-deficient macrophages, indicating that cell death occurred independently of caspase-1. Analysis of KIM-derived strains defective for production of functional effector or translocator Yops indicated that translocation of catalytically active YopJ into macrophages was required for caspase-1 activation and cell death. Release of LDH and secretion of IL-1beta were not reduced when actin polymerization was inhibited in KIM5-infected macrophages, indicating that extracellular bacteria translocating YopJ could trigger cell death and caspase-1 activation. This study uncovered a novel role for YopJ in the activation of caspase-1 in macrophages.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Lilo S,Zheng Y,Bliska JB

doi

10.1128/IAI.01695-07

subject

Has Abstract

pub_date

2008-09-01 00:00:00

pages

3911-23

issue

9

eissn

0019-9567

issn

1098-5522

pii

IAI.01695-07

journal_volume

76

pub_type

杂志文章
  • Antibody synthesis specific for nonoral antigens in inflamed gingiva.

    abstract::In vitro experimentation indicates that periodontitis-associated bacteria contain potent polyclonal B-cell activators (PBA). We reasoned that if PBA were operative in vivo, plasma cells specific for nonoral antigens should be present in the inflamed gingival tissues, which are characterized by a plasma cell infiltrate...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.56.4.823-830.1988

    authors: Mallison SM 3rd,Szakal AK,Ranney RR,Tew JG

    更新日期:1988-04-01 00:00:00

  • A Stenotrophomonas maltophilia Strain Evades a Major Caenorhabditis elegans Defense Pathway.

    abstract::Stenotrophomonas maltophilia is a ubiquitous bacterium and an emerging nosocomial pathogen. This bacterium is resistant to many antibiotics, associated with a number of infections, and a significant health risk, especially for immunocompromised patients. Given that Caenorhabditis elegans shares many conserved genetic ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00711-15

    authors: White CV,Darby BJ,Breeden RJ,Herman MA

    更新日期:2015-12-07 00:00:00

  • Evaluation of an isogenic major outer membrane protein-deficient mutant in the human model of Haemophilus ducreyi infection.

    abstract::Haemophilus ducreyi expresses 2 OmpA homologs, designated MOMP and OmpA2, whose genes are arranged in tandem on the chromosome. Northern blot analysis indicated that momp and ompA2 are transcribed independently. Sequences of the momp open reading frame (ORF) lacking the transcriptional start site were amplified by PCR...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.5.2602-2607.2000

    authors: Throm RE,Al-Tawfiq JA,Fortney KR,Katz BP,Hood AF,Slaughter CA,Hansen EJ,Spinola SM

    更新日期:2000-05-01 00:00:00

  • Mechanism of membrane damage by streptolysin-O.

    abstract::Streptolysin-O (SLO) is a thiol-activated, membrane-damaging protein toxin of Mr 69,000 that is produced by most strains of beta-hemolytic group A streptococci. Native, primarily water-soluble toxin molecules bind to cholesterol-containing target membranes to assemble into supramolecular curved rod structures (25 to 1...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.47.1.52-60.1985

    authors: Bhakdi S,Tranum-Jensen J,Sziegoleit A

    更新日期:1985-01-01 00:00:00

  • Effect of peroxisome proliferator-activated receptor alpha activators on tumor necrosis factor expression in mice during endotoxemia.

    abstract::Inflammatory mediators orchestrate the host immune and metabolic response to acute bacterial infections and mediate the events leading to septic shock. Tumor necrosis factor (TNF) has long been identified as one of the proximal mediators of endotoxin action. Recent studies have implicated peroxisome proliferator-activ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.67.7.3488-3493.1999

    authors: Hill MR,Clarke S,Rodgers K,Thornhill B,Peters JM,Gonzalez FJ,Gimble JM

    更新日期:1999-07-01 00:00:00

  • Immunoglobulin E, a pathogenic factor in Plasmodium falciparum malaria.

    abstract::Most children and adults living in areas where the endemicity of Plasmodium falciparum malaria is high have significantly elevated levels of both total immunoglobulin E (IgE) and IgE antimalarial antibodies in blood. This elevation is highest in patients with cerebral malaria, suggesting a pathogenic role for this imm...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.1.116-121.1997

    authors: Perlmann P,Perlmann H,Flyg BW,Hagstedt M,Elghazali G,Worku S,Fernandez V,Rutta AS,Troye-Blomberg M

    更新日期:1997-01-01 00:00:00

  • Regulation of mycobacterial growth by the hypothalamus-pituitary-adrenal axis: differential responses of Mycobacterium bovis BCG-resistant and -susceptible mice.

    abstract::The role of the hypothalamus-pituitary-adrenal (HPA) axis in regulating the growth of Mycobacterium avium in Mycobacterium bovis BCG-resistant and -susceptible congenic mice was evaluated. Restraint was used to activate the HPA axis, which resulted in an increase in the level of corticosterone in the plasma. Activatio...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.61.11.4793-4800.1993

    authors: Brown DH,Sheridan J,Pearl D,Zwilling BS

    更新日期:1993-11-01 00:00:00

  • Differential patterns of liver proteins in experimental murine hepatosplenic schistosomiasis.

    abstract::Schistosoma mansoni eggs produced by adult worms in the mesenteric vasculature become trapped in the liver, where they induce granulomatous lesions and strong immune responses. Infected individuals suffer from intestinal schistosomiasis (INT) in 90% of cases, whereas the remaining 10% present with severe hepatosplenic...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00647-09

    authors: Manivannan B,Rawson P,Jordan TW,Secor WE,La Flamme AC

    更新日期:2010-02-01 00:00:00

  • Production and partial characterization of a Vibrio fluvialis cytotoxin.

    abstract::Conditions are described for the production of an extracellular cytotoxin or CHO cell-killing factor by Vibrio fluvialis, a recently recognized enteric pathogen. The cell-killing factor was ammonium sulfate precipitable, heat labile, and inactivated by proteases, and had an isoelectric point (estimated by sucrose dens...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.46.3.773-777.1984

    authors: Wall VW,Kreger AS,Richardson SH

    更新日期:1984-12-01 00:00:00

  • Host and tissue specificity of Trichomonas vaginalis is not mediated by its known adhesion proteins.

    abstract::Adhesion of Trichomonas vaginalis is believed to be dependent on four adhesion proteins, which are thought to bind to vaginal epithelial cells in a specific manner with a ligand-receptor type of interaction. However, the specific receptors on the host cell have not yet been identified. In this work, the ability of the...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.7.4358-4360.2000

    authors: Addis MF,Rappelli P,Fiori PL

    更新日期:2000-07-01 00:00:00

  • A recombinant Rickettsia conorii vaccine protects guinea pigs from experimental boutonneuse fever and Rocky Mountain spotted fever.

    abstract::There are no vaccines against boutonneuse fever and Rocky Mountain spotted fever. Previous studies have identified a Rickettsia rickettsii surface protein as a vaccine candidate and shown that an antigenically related protein is present in R. conorii, which causes boutonneuse fever. The gene encoding the R. rickettsii...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.3.646-653.1990

    authors: Vishwanath S,McDonald GA,Watkins NG

    更新日期:1990-03-01 00:00:00

  • Increased content of microbicidal cationic peptides in rabbit alveolar macrophages elicited by complete Freund adjuvant.

    abstract::We measured the microbicidal peptides MCP-1 and MCP-2 in rabbit alveolar macrophages (AM), comparing rabbits pretreated with complete Freund adjuvant with untreated control animals. Levels of MCP-1 increased from 4.7 +/- 0.6 microgram/10(7) resident AM to 13.2 +/- 0.1 microgram/10(7) complete Freund adjuvant-elicited ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.33.3.775-778.1981

    authors: Lehrer RI,Szklarek D,Selsted ME,Fleischmann J

    更新日期:1981-09-01 00:00:00

  • Treatment of alveolar macrophages with cytochalasin D inhibits uptake and subsequent growth of Legionella pneumophila.

    abstract::Legionella pneumophila multiplied rapidly in guinea pig and rat alveolar macrophages but failed to grow when phagocytic activity was inhibited by pretreatment with 0.5 or 1.0 microgram of cytochalasin D per ml. Attachment was not inhibited by cytochalasin D. No extracellular multiplication occurred when L. pneumophila...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.51.1.31-36.1986

    authors: Elliott JA,Winn WC Jr

    更新日期:1986-01-01 00:00:00

  • Differential regulation of in vitro humoral and cellular immune responsiveness in Brugia pahangi-infected jirds.

    abstract::Patent filarial infection is associated with the downregulation of parasite-specific immune reactivity. In the present study, the relationship between in vitro parasite antigen-specific humoral and cellular immune responsiveness was investigated in Brugia pahangi-infected jirds and in jirds immunized with soluble anti...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.56.12.3052-3057.1988

    authors: Prier RC,Lammie PJ

    更新日期:1988-12-01 00:00:00

  • Beneficial effects of interleukin-6 in neonatal mouse models of group B streptococcal disease.

    abstract::Previous studies have shown that tumor necrosis factor alpha (TNF-alpha) plays a pathophysiologic role in sepsis induced in rat pups by group B streptococci (GBS). In this model, TNF-alpha is also partially responsible for the induction of interleukin-6 (IL-6). The present study was undertaken to investigate the role ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.62.11.4997-5002.1994

    authors: Mancuso G,Tomasello F,Migliardo M,Delfino D,Cochran J,Cook JA,Teti G

    更新日期:1994-11-01 00:00:00

  • The central variable V2 region of the CS31A major subunit is involved in the receptor-binding domain.

    abstract::CS31A is a K88-related capsule-like surface protein that mediates Escherichia coli and Klebsiella pneumoniae adhesion to the human Caco-2 and Intestine-407 cell lines. In this study, we demonstrate that ClpG, the major subunit of CS31A, contains the adhesive domain of the polymerized structure. We mapped this domain w...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.2.609-616.1997

    authors: Di Martino P,Girardeau JP,Der Vartanian M,Joly B,Darfeuille-Michaud A

    更新日期:1997-02-01 00:00:00

  • Levels of inhibitors of tumor necrosis factor alpha and interleukin 1beta in urine and sera of patients with urosepsis.

    abstract::The antiinflammatory cytokine response during urosepsis was determined by measurement of concentrations of soluble tumor necrosis factor receptor (sTNFR) types I and II, interleukin 1 receptor antagonist (IL-1ra), soluble IL-1 receptor type II (sIL-1RII), and interleukin 10 in sera and urine of 30 patients with cultur...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.66.8.3527-3534.1998

    authors: Olszyna DP,Prins JM,Buis B,van Deventer SJ,Speelman P,van der Poll T

    更新日期:1998-08-01 00:00:00

  • Complete protection against lethal Toxoplasma gondii infection in mice immunized with a plasmid encoding the SAG1 gene.

    abstract::Infection with the protozoan parasite Toxoplasma gondii is transmitted to humans from infected animals by tissue cysts and oocysts excreted by cats. Immunization with inactivated parasites or recombinant proteins has at best shown partial protection. We constructed a plasmid expressing the SAG1 surface antigen of T. g...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:

    authors: Nielsen HV,Lauemøller SL,Christiansen L,Buus S,Fomsgaard A,Petersen E

    更新日期:1999-12-01 00:00:00

  • Chemiluminescence by human alveolar macrophages: stimulation with heat-killed bacteria or phorobol myristate acetate.

    abstract::Chemiluminescence of human alveolar macrophages (AM) was evaluated in vitro. Unstimulated AM generated chemiluminescence that remained constant during incubation. Addition of heat-killed Staphylococcus aureus 502A (HKB) or a chemical agent, phorbol myristate acetate, produced high rates of chemiluminescence that were ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.17.1.117-120.1977

    authors: Beall GD,Repine JE,Hoidal JR,Rasp FL

    更新日期:1977-07-01 00:00:00

  • Mycolic acids from "noncultivable" mycobacteria.

    abstract::Chromatographic analysis, coupled to mass spectrometry with a high-resolution mass spectrometer, of materials isolated from skin lesions of patients with lepromatous leprosy allows the recognition of characteristic mycobacterial products, mycolic acids. This finding indicates that the "noncultivable" bacteria responsi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.10.1.236-239.1974

    authors: Etemadi AH,Convit J

    更新日期:1974-07-01 00:00:00

  • Bacteriophage MAV1 is not associated with virulence of Mycoplasma arthritidis.

    abstract::Previous studies demonstrated that Mycoplasma arthritidis strain 158 acquired a high degree of virulence upon lysogenization with bacteriophage MAV1. In the present study, the association between MAV1 and virulence was reexamined by creating new lysogens of 158 and of a relatively avirulent mutant, strain 158-1. In th...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.72.12.7322-7325.2004

    authors: Clapper B,Tu AH,Simmons WL,Dybvig K

    更新日期:2004-12-01 00:00:00

  • Melanin from Fonsecaea pedrosoi induces production of human antifungal antibodies and enhances the antimicrobial efficacy of phagocytes.

    abstract::Fonsecaea pedrosoi is a fungal pathogen that produces melanin. The functions of melanin and its possible influence in the protective immunological response during infection by F. pedrosoi are not known. In this work, treatment of F. pedrosoi mycelia with proteases and glycosidases followed by a denaturing agent and ho...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.72.1.229-237.2004

    authors: Alviano DS,Franzen AJ,Travassos LR,Holandino C,Rozental S,Ejzemberg R,Alviano CS,Rodrigues ML

    更新日期:2004-01-01 00:00:00

  • Synthetic polysaccharide type 3-related di-, tri-, and tetrasaccharide-CRM(197) conjugates induce protection against Streptococcus pneumoniae type 3 in mice.

    abstract::Di-, tri-, and tetrasaccharides, synthesized according to the chemical structure of pneumococcal polysaccharide type 3 (PS3), were coupled to the cross-reactive material (CRM(197)) of modified diphtheria toxin in different molar carbohydrate/protein ratios using the squarate coupling method. To study protective immuni...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.7.4698-4701.2001

    authors: Benaissa-Trouw B,Lefeber DJ,Kamerling JP,Vliegenthart JF,Kraaijeveld K,Snippe H

    更新日期:2001-07-01 00:00:00

  • Avirulent uracil auxotrophs based on disruption of orotidine-5'-monophosphate decarboxylase elicit protective immunity to Toxoplasma gondii.

    abstract::The orotidine-5'-monophosphate decarboxylase (OMPDC) gene, encoding the final enzyme of the de novo pyrimidine biosynthesis pathway, was deleted using Toxoplasma gondii KU80 knockouts to develop an avirulent nonreverting pyrimidine auxotroph strain. Additionally, to functionally address the role of the pyrimidine salv...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00287-10

    authors: Fox BA,Bzik DJ

    更新日期:2010-09-01 00:00:00

  • Role of interleukin-1 in the depression of liver drug metabolism by endotoxin.

    abstract::Endotoxin-resistant C3H/HeJ mice were used to test the hypothesis that a macrophage product, possibly interleukin-1, might mediate the depression of liver cytochrome P-450-dependent drug metabolism in endotoxin-treated mice. Depression of liver drug metabolism by endotoxin was observed in normal mice (C3H/HeN) but not...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.54.3.837-840.1986

    authors: Ghezzi P,Saccardo B,Villa P,Rossi V,Bianchi M,Dinarello CA

    更新日期:1986-12-01 00:00:00

  • Scanning electron microscopy of epidermal adherence and cavitation in murine candidiasis: a role for Candida acid proteinase.

    abstract::Adherence of blastoconidia to epidermal corneocytes is an early event in Candida colonization and infection of the skin. Pathogenic species adhere more avidly than nonpathogenic species, transform to hyphal growth, and invade the stratum corneum of the skin. Adherence was studied by scanning electron microscopy of exp...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.56.8.1942-1949.1988

    authors: Ray TL,Payne CD

    更新日期:1988-08-01 00:00:00

  • In vivo behavior of revertants from Stahylococcus aureus L-phase variants compared with the parent strain.

    abstract::The in vivo behavior of revertants of Staphylococcus aureus L-phase variants were studied in mice. The Giorgio strain of S. aureus was induced into L-phase variants by methicillin. After 13 serial passages in methicillin agar and six passages in antibiotic-free agar, reversion occurred. Six revertants, all phage untyp...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.11.6.1182-1186.1975

    authors: Watanakunakorn C,Glotzbecker C

    更新日期:1975-06-01 00:00:00

  • Vaccination with Mycobacterium bovis BCG affects the distribution of Fc receptor-bearing T lymphocytes in experimental pulmonary tuberculosis.

    abstract::Inbred strain 2 guinea pigs were vaccinated with Mycobacterium bovis BCG or were left unvaccinated and challenged 6 weeks later by the respiratory route with virulent Mycobacterium tuberculosis. By using a double rosette assay with isotype-specific antibody-coated ox and uncoated rabbit erythrocytes, the proportions o...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.57.5.1374-1379.1989

    authors: Bartow RA,McMurray DN

    更新日期:1989-05-01 00:00:00

  • Inhibition of phagocytosis by Haemophilus ducreyi requires expression of the LspA1 and LspA2 proteins.

    abstract::Haemophilus ducreyi previously has been shown to inhibit the phagocytosis of both secondary targets and itself by certain cells in vitro. Wild-type H. ducreyi strain 35000HP contains two genes, lspA1 and lspA2, whose encoded protein products are predicted to be 456 and 543 kDa, respectively. An isogenic mutant of H. d...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.10.5994-6003.2003

    authors: Vakevainen M,Greenberg S,Hansen EJ

    更新日期:2003-10-01 00:00:00

  • Differentiation of F38 mycoplasmas causing contagious caprine pleuropneumonia with a growth-inhibiting monoclonal antibody.

    abstract::Monoclonal antibody WM-25 inhibited the in vitro growth of 13 F38 isolates from goats with contagious caprine pleuropneumonia but not 7 heterologous mycoplasma isolates representing four different species. In contrast to results with polyclonal antisera, growth inhibition by monoclonal antibody WM-25 was specific for ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.55.12.3219-3220.1987

    authors: Rurangirwa FR,McGuire TC,Musoke AJ,Kibor A

    更新日期:1987-12-01 00:00:00