Abstract:
:Brucella abortus endotoxin preparations, containing approximately 5 to 6% protein, induce strong immune and adjuvant immunoglobulin G (IgG) responses as compared with Escherichia coli endotoxin preparations, with equivalent amounts of protein, which induce responses in which IgM antibody predominates. Using an enzyme-linked immunoassay with isotype-specific conjugates, we found that antibody of all four subclasses of IgG were evoked during the course of the immune responses of C3H/HeAu mice to B. abortus endotoxin. Secondary responses of endotoxin-hyporesponsive C3H/HeJ mice were similar to those seen in C3H/HeAu mice, although lower levels of antibody were produced during their primary responses. The primary responses of BALB/c athymic mice consisted almost entirely of IgG3, and IgG1 appeared following a second injection. The effects of lipopolysaccharide (LPS)-associated protein on the immunogenic properties of B. abortus endotoxin were examined by comparing responses to endotoxin with those to a purified B. abortus LPS containing less than 1% protein. The endotoxin evoked strong primary and secondary responses in which antibody directed to LPS determinants consisted mainly of IgG3 and those to the protein determinants were largely IgG1 antibody. Primary and secondary responses to purified LPS consisted mainly of IgG3 antibody. The potential mechanism of the contribution of protein to the immunogenic properties of the endotoxin as well as possible immune mechanisms involved in these responses are discussed.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Kurtz RS,Berman DTdoi
10.1128/IAI.54.3.728-734.1986subject
Has Abstractpub_date
1986-12-01 00:00:00pages
728-34issue
3eissn
0019-9567issn
1098-5522journal_volume
54pub_type
杂志文章abstract::Cryptococcus neoformans NIH 409 was cultured in a defined medium containing D-[1-13C]xylose (Xyl), D-[1-13C]mannose (Man), or D-[1-13C]mannitol as the sole carbon source. The distribution of 13C in the Man, Xyl, glucuronic acid (GlcA), and O-acetyl constituents of native and de-O-acetylated glucuronoxylomannan (GXM) w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2996-2998.1998
更新日期:1998-06-01 00:00:00
abstract::In vitro, macrophages from normal strain BRVR mice killed Salmonella more quickly than did macrophages from normal strain BSVS mice. Salmonella injected intraperitoneally multiplied more quickly in BSVS mice than in BRVR mice. BRVR macrophages injected intraperitoneally into BSVS mice protected against Salmonella mult...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.4.438-443.1972
更新日期:1972-10-01 00:00:00
abstract::We are currently studying the soluble egg antigens of Schistosoma japonicum in an attempt to determine which antigens are potent immunogens. Previously, we demonstrated by Ouchterlony immunodiffusion and inhibition of the circumoval precipitin test that the glycoprotein fraction of soluble egg antigens contains the an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.2.397-406.1981
更新日期:1981-11-01 00:00:00
abstract::Severe combined immunodeficient beige mice were inoculated orally and intraperitoneally with a bovine strain of Mycobacterium paratuberculosis to explore their potential as laboratory animal models in the study of paratuberculosis (Johne's disease). Control animals were similarly inoculated with heat-killed M. paratub...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.10.4074-4079.1992
更新日期:1992-10-01 00:00:00
abstract::Dendritic cells (DC) suffer a maturation defect following interaction with erythrocytes infected with malaria parasites and become unable to induce protective malaria liver-stage immunity. Here we show that, by contrast, maturation-arrested DC in vitro are capable of the successful induction of antigen-specific gamma ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.9.5331-5339.2004
更新日期:2004-09-01 00:00:00
abstract::It has been postulated that systemic cell-mediated immunity (CMI) is an important host defense mechanism against Candida infections of the vagina. However, in an estrogen-dependent murine model of experimental vaginal candidiasis, we recently showed that systemic Candida-specific Th1-type CMI induced by immunization w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.2.547-553.1995
更新日期:1995-02-01 00:00:00
abstract::Coxiella burnetii is the causative agent of Q fever, a zoonotic disease that threatens both human and animal health. Due to the paucity of experimental animal models, little is known about how host factors interface with bacterial components and affect pathogenesis. Here, we used Drosophila melanogaster, in conjunctio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00218-17
更新日期:2017-06-20 00:00:00
abstract::Purified cell walls representing a wide variety in teichoic acid and peptidoglycan structure prepared from eight different gram-positive bacterial species induced the production of tumor necrosis factor alpha (TNF-alpha) and interleukin-6 from human monocytes in the presence of 10% plasma or serum. Significant amounts...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.7.2715-2721.1994
更新日期:1994-07-01 00:00:00
abstract::Staphylococcus aureus nasal carriage is a risk factor for infection in humans, particularly in the hospital environment. Attenuation of carriage has proven effective in reducing the prevalence of infection in some high-risk groups. To study staphylococcal factors that influence nasal colonization, a mouse model of S. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5001-5006.1999
更新日期:1999-10-01 00:00:00
abstract::During a Plasmodium chabaudi infection in interleukin-10 (IL-10) knockout mice, there is greater parasite sequestration, more severe cerebral edema, and a high frequency of cerebral hemorrhage compared with infection of C57BL/6 mice. Anti-tumor necrosis factor alpha treatment ameliorated both cerebral edema and hemorr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.5.3054-3058.2004
更新日期:2004-05-01 00:00:00
abstract::Oral or intravenous inoculation of previously unexposed juvenile and adult ferrets with Campylobacter jejuni uniformly resulted in intestinal colonization lasting 2 to 12 days. Disease varied from mild to moderate diarrhea, which resolved in 2 to 3 days. Orally infected animals developed agglutinin titers of 8 to 256 ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1848-1852.1990
更新日期:1990-06-01 00:00:00
abstract::The acute phase of Chagas' disease is accompanied by immunosuppression. To explore the underlying mechanism(s), we used an in vitro culture system in which the capacities of activated human peripheral blood mononuclear cells to express interleukin-2 receptors (IL-2R) and proliferate are markedly inhibited in the prese...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.8.2301-2305.1989
更新日期:1989-08-01 00:00:00
abstract::Gamma interferon (IFN-gamma)-primed human gingival fibroblasts (HGF) have been shown to produce higher levels of interleukin-8 (IL-8) upon stimulation with bacterial products and inflammatory cytokines than nonprimed controls. In this study, we examined whether priming of HGF with IFN-gamma up-regulates IL-8 productio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1272-1278.2002
更新日期:2002-03-01 00:00:00
abstract::Previous studies found that normal human serum (NHS) contains an immunoglobulin G (IgG) antibody that mediates initiation of the classical complement pathway by nonencapsulated Cryptococcus neoformans. The present study used an enzyme-linked immunosorbent assay with whole nonencapsulated yeast cells as solid-phase ant...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.1.215-220.1994
更新日期:1994-01-01 00:00:00
abstract::The oral streptococcal group (mitis phylogenetic group) currently consists of nine recognized species, although the group has been traditionally difficult to classify, with frequent changes in nomenclature over the years. The pneumococcus (Streptococcus pneumoniae), an important human pathogen, is traditionally distin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1374-1382.2000
更新日期:2000-03-01 00:00:00
abstract::Bordetella pertussis fimbriae are composed of a major subunit, Fim2 or Fim3, and the minor subunit FimD. Using immunoelectron microscopy, we provide evidence that FimD is located at the fimbrial tip. The role of FimD in colonization of the mouse respiratory tract was studied by using two fimbrial mutants: a mutant com...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4222-4228.1997
更新日期:1997-10-01 00:00:00
abstract::Yersinia enterocolitica serotype 9 contained an antigenic component giving a reaction of total identity with Brucella native hapten and polysaccharide B. This component was present in a phenol-water extract (fraction 5; M. Redfearn, Ph.D. Thesis, University of Wisconsin, Madison, 1960) along with the smooth lipopolysa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.2.778-780.1982
更新日期:1982-11-01 00:00:00
abstract::The rationale for the present study was to determine how different species of bacteria interact with cells of the human meninges in order to gain information that would have broad relevance to understanding aspects of the innate immune response in the brain. Neisseria lactamica is an occasional cause of meningitis in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00644-06
更新日期:2006-11-01 00:00:00
abstract::Proteins were extracted at pH 2.5 from carefully washed brain homogenates of patients with subacute sclerosing panencephalitis. Immunoglobulin G was isolated from the extracts by gel filtration and immunoadsorbent chromatography and was 125I radioiodinated. The labeled immunoglobulin G was reacted with purified measle...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.202-210.1979
更新日期:1979-04-01 00:00:00
abstract::The cytokine response to the agent of human granulocytic ehrlichiosis (HGE) was assessed in a murine infection model and the role of gamma interferon (IFN-gamma), a cytokine that is crucial for host defenses against intracellular pathogens, was investigated by using IFN-gamma-deficient mice. The agent of HGE (aoHGE) i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.1827-1833.2000
更新日期:2000-04-01 00:00:00
abstract::Minimally manipulated nasal secretions, an accessible form of airway surface fluid, were tested against indigenous and added bacteria by using CFU assays. Antimicrobial activity was found to vary between donors and with different target bacteria and was markedly diminished by dilution of the airway secretions. Donor-t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.7.3267-3275.1999
更新日期:1999-07-01 00:00:00
abstract::We identified a gene (atlA) encoding autolytic activity from Streptococcus mutans Xc. The AtlA protein predicted to be encoded by atlA is composed of 979 amino acids with a molecular weight of 107,279 and has a conserved beta-1,4-N-acetylmuramidase (lysozyme) domain in the C-terminal portion. Sodium dodecyl sulfate ex...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3512-3520.2005
更新日期:2005-06-01 00:00:00
abstract::Theileria parasites infect and transform bovine leukocytes. We have analyzed laboratory-established Theileria sp.-infected leukocyte lines and observed that transformed macrophages express CD5. Low-level expression of CD5 by macrophages was further confirmed on three independent Theileria annulata clinical isolates fr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Despite effective antimicrobial chemotherapy, control of Chlamydia trachomatis urogenital infection will likely require a vaccine. We have assessed the protective effect of an outer membrane protein-based vaccine by using a murine model of chlamydial genital infection. Female mice were first vaccinated with Chlamydia ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00622-10
更新日期:2010-10-01 00:00:00
abstract::In the presence of temporary obstruction (20 h), ascending Escherichia coli urinary infection leads to irreversible acute exudative pyelonephritis (AEP) in rats. The purpose of the present study was to investigate the early inflammatory events which take place in response to the presence of bacteria in the kidney pare...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.2.529-533.1983
更新日期:1983-05-01 00:00:00
abstract::Staphylococcus aureus and Salmonella enterica serovar Dublin invade osteoblasts and are causative agents of human bone disease. In the present study, we examined the ability of S. aureus and Salmonella serovar Dublin to induce the production of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) by normal ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.3.1581-1586.2001
更新日期:2001-03-01 00:00:00
abstract::CXCL16 is a recently discovered multifaceted chemokine that has been shown not only to recruit activated T lymphocytes but also to play a direct role in the binding and phagocytosis of bacteria by professional antigen-presenting cells. In this study, we investigated the role of CXCL16 in vivo in the regulation of the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01065-06
更新日期:2006-12-01 00:00:00
abstract::Pyolysin (PLO), the hemolytic exotoxin expressed by Arcanobacterium (Actinomyces) pyogenes, is a member of the thiol-activated cytolysin family of bacterial toxins. Insertional inactivation of the plo gene results in loss of expression of PLO with a concomitant loss in hemolytic activity. The plo mutant, PLO-1, has an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::Conjugated polysaccharide vaccines protect against serogroup C meningococci. However, this approach cannot be applied to serogroup B, which is still a major cause of meningitis. We evaluated the immunogenicity of three surface-exposed proteins from serogroup B Neisseria meningitidis (App, NhhA, and NadA) identified du...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.4052-4060.2004
更新日期:2004-07-01 00:00:00
abstract::Experimental infections of mice with the African trypanosome Trypanosoma brucei lead to a profound state of T-cell unresponsiveness in the lymph node cell (LNC) compartment. This suppression is mediated by macrophage-like cells which inhibit interleukin 2 (IL-2) secretion and down-regulate IL-2 receptor expression (M....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.1937-1943.1996
更新日期:1996-06-01 00:00:00