Abstract:
:Despite effective antimicrobial chemotherapy, control of Chlamydia trachomatis urogenital infection will likely require a vaccine. We have assessed the protective effect of an outer membrane protein-based vaccine by using a murine model of chlamydial genital infection. Female mice were first vaccinated with Chlamydia muridarum major outer membrane protein (MOMP) plus the adjuvants CpG-1826 and Montanide ISA 720; then they were challenged with C. muridarum. Vaccinated mice shed 2 log(10) to 3 log(10) fewer inclusion-forming units (IFU) than ovalbumin-vaccinated or naïve animals, resolved infection sooner, and had a lower incidence of hydrosalpinx. To determine the relative contribution of T cells to vaccine-induced protection, mice were vaccinated, depleted of CD4(+) or CD8(+) T cells, and then challenged vaginally with C. muridarum. Depletion of CD4(+) T cells, but not depletion of CD8(+) T cells, diminished vaccine-induced protection, with CD4-depleted mice shedding 2 log(10) to 4 log(10) more IFU than CD8-depleted or nondepleted mice. The contribution of antibodies to vaccine-induced protection was demonstrated by the absence of protective immunity in vaccinated B-cell-deficient mice and by a 2 log(10) to 3 log(10) decrease in bacterial shedding by mice passively administered an anti-MOMP serum. Thus, optimal protective immunity in this model of vaccine-induced protection depends on contributions from both CD4(+) T cells and antibody.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Farris CM,Morrison SG,Morrison RPdoi
10.1128/IAI.00622-10subject
Has Abstractpub_date
2010-10-01 00:00:00pages
4374-83issue
10eissn
0019-9567issn
1098-5522pii
IAI.00622-10journal_volume
78pub_type
杂志文章abstract::A hemolysin that appears to be responsible for the zones of beta-hemolysis surrounding colonies of group B streptococci (Streptococcus agalactiae) on blood agar plates has been isolated and partially purified. No soluble hemolysin was detectable in the supernatants of streptococcal cultures grown in several types of m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-12-01 00:00:00
abstract::The role of receptor binding in the toxicity, immunogenicity, and adjuvanticity of the heat-labile enterotoxin of Escherichia coli (LT) was examined by comparing native LT and LT(G33D), a B-subunit receptor binding mutant, with respect to the ability to bind to galactose and to GM1, toxicity on mouse Y-1 adrenal tumor...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.12.4943-4950.1997
更新日期:1997-12-01 00:00:00
abstract::Rubella virus was capable of replicating in both unstimulated and phytohemagglutinin-stimulated cultures of human mononuclear blood cells. Monocyte-derived macrophages were the main cell type responsible for viral replication. The susceptibility of macrophages increased during cultivation. Phytohemagglutinin-stimulate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.309-314.1980
更新日期:1980-02-01 00:00:00
abstract::Highly purified protein antigens are usually poor immunogens; in practice, adjuvants are needed to obtain satisfactory immune responses. Plasmodium yoelii 19-kDa merozoite surface protein 1 (MSP1(19)) is a weak antigen, but mice vaccinated with this antigen in strong adjuvants can survive an otherwise lethal parasite ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01369-07
更新日期:2008-08-01 00:00:00
abstract::This study was undertaken to determine the sequence of events in the microenvironment of the intestinal tract that culminate in the symptoms of cholera and to attempt to define more clearly the mechanisms involved in antibacterial immunity. The extent to which mucus occurs in the normal intestine of rabbits and the ap...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.1.195-203.1976
更新日期:1976-01-01 00:00:00
abstract::Urease activity has recently been shown to be an important virulence determinant for Helicobacter pylori, allowing it to survive the low pH of the stomach during colonization. Experimental murine infection with Helicobacter felis is now being used as a model for H. pylori infection to study the effects of vaccines, an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.3.793-798.1994
更新日期:1994-03-01 00:00:00
abstract::The protein P2 comprises a large proportion of the outer membrane of nontypable Haemophilus influenzae and functions as a porin. In view of the importance of the protein as a surface antigen, the present study was designed to purify and analyze P2 with particular emphasis on detection of antigenic determinants express...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.5.1084-1089.1988
更新日期:1988-05-01 00:00:00
abstract::Cronobacter sakazakii is an opportunistic pathogen that causes neonatal meningitis and necrotizing enterocolitis. Its interaction with intestinal epithelium is important in the pathogenesis of enteric infections. In this study, we investigated the involvement of the inv gene in the virulence of C. sakazakii ATCC 29544...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01397-13
更新日期:2014-05-01 00:00:00
abstract::Ribosomal subunits from Mycobacterium smegmatis were analyzed by using sedimentation velocity, sedimentation equilibrium, and acrylamide gel electrophoresis experiments. These s(20.w) values for the subunits are 48.7S and 28.1S. The molecular weight of the 49S subunit is about 1.65 x 10(6), and that of the 28S subunit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.3.489-496.1974
更新日期:1974-03-01 00:00:00
abstract::We undertook a morphological and immunochemical comparison of purified outer membrane antigens from oral and nonoral isolates of Bacteroides asaccharolyticus. Electron micrographs of thin sections of whole bacteria revealed a compact, electron-dense capsule external to the outer membrane of oral strains. A loose, web-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.589-596.1980
更新日期:1980-02-01 00:00:00
abstract::Borrelia burgdorferi encodes a functional homolog of canonical Lon protease termed Lon-2. In addition, B. burgdorferi encodes a second Lon homolog called Lon-1. Recent studies suggest that Lon-1 may function differently from the prototypical Lon protease. However, the function of Lon-1 in B. burgdorferi biology remain...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00951-19
更新日期:2020-05-20 00:00:00
abstract::Murine cytomegalovirus pools from various in vitro and in vivo sources were compared for virulence in suckling mice in an effort to identify the conditions which were necessary for the production of virulent and attenuated viruses. Virus passaged in tracheal ring and salivary gland organ cultures, where virus is produ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.3.840-847.1981
更新日期:1981-09-01 00:00:00
abstract::An inactivated oral enterotoxigenic Escherichia coli (ETEC) vaccine against ETEC diarrhea was given to 25 adult Swedish volunteers. The vaccine consisted of formalin-killed E. coli bacteria expressing the most common colonization factor antigens (CFAs), i.e., CFA/I, -II, and -IV, and recombinantly produced cholera B s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.7.3311-3316.1998
更新日期:1998-07-01 00:00:00
abstract::Genetically based natural resistance to brucellosis in cattle provides for novel strategies to control zoonotic diseases. Bovine NRAMP1, the homologue of a murine gene (Bcg), has been identified as a major candidate for controlling the in vivo resistant phenotype. We developed an in vitro model for expression of resis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3110-3119.2001
更新日期:2001-05-01 00:00:00
abstract::The adherence protein (P1 protein) of Mycoplasma pneumoniae was purified by electroelution and cleaved with cyanogen bromide. The resulting peptides were separated by two-dimensional electrophoresis. Spots reacting in Western immunoblots with two attachment-inhibiting monoclonal antibodies were isolated, and the amino...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.3.685-688.1989
更新日期:1989-03-01 00:00:00
abstract::A clinically isolated strain of polymorphonuclear leukocyte (PMN)-resistant Pseudomonas was found to produce an extracellular substance (PMN inhibitor) that inhibits the phagocytic and killing activities of PMN. The PMN inhibitor was purified from culture filtrates by precipitation with (NH4)2SO4 and chromatography on...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.2.394-398.1979
更新日期:1979-05-01 00:00:00
abstract::The selection of influenza B virus recombinants from plaques in bovine kidney cell monolayers is described. Two sets of recombinants were each derived from parents of high and low virulence for humans, respectively. Recombination frequency was apparently high, and reassortment of genes made it possible to obtain atten...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.2.347-353.1977
更新日期:1977-02-01 00:00:00
abstract::Although excystation is crucial to the initiation of infection by Giardia lamblia, little is known about the regulation of this important process. We have been able to reliably induce excystation in vitro by mimicking cyst passage through the stomach and upper small intestine by the exposure of in vitro-derived cysts ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2151-2157.1996
更新日期:1996-06-01 00:00:00
abstract::Indoleamine 2,3-dioxygenase (IDO), a tryptophan-degrading enzyme, is inducible by various interferons (IFNs). IDO-mediated tryptophan degradation, but not the formation of IDO-catalyzed tryptophan metabolites, has been suggested as a mechanism for the antiparasitic action of IFN-gamma. To determine whether the IFN-gam...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.5.1810-1813.1993
更新日期:1993-05-01 00:00:00
abstract::We examined 205 enterotoxigenic strains of Escherichia coli for colonization factor antigens (CFA) I and II, using an immunodiffusion technique with specific antisera. A total of 36 strains of serogroups O63, O78, O114, O128, and O153 and 1 rough strain possessed CFA/I and gave a single precipitin line; 47 strains of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.1.189-197.1982
更新日期:1982-04-01 00:00:00
abstract::The gene encoding Clostridium sordellii phospholipase C (Csp) was cloned and expressed as a histidine-tagged (His-tag) protein, and the protein was purified to compare its enzymatic and biological activities with those of Clostridium perfringens phospholipase C (Cpa) and Clostridium bifermentans phospholipase C (Cbp)....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.2.641-646.2003
更新日期:2003-02-01 00:00:00
abstract::The production of delta-toxin is supposed to be responsible for various pathophysiological effects during infection with Staphylococcus aureus. We compared the effects of delta-toxin with the structurally related bee venom toxin melittin on granulocyte functions and inflammatory mediator release. Delta-toxin and melit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1653-1659.1990
更新日期:1990-06-01 00:00:00
abstract::The obligate intracellular parasite Toxoplasma gondii can actively infect any nucleated cell type, including cells from the immune system. The rapid transfer of T. gondii from infected dendritic cells to effector natural killer (NK) cells may contribute to the parasite's sequestration and shielding from immune recogni...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00069-17
更新日期:2017-09-20 00:00:00
abstract::Relapsing fever (RF) is a spirochetal infection characterized by relapses of a febrile illness and spirochetemia due to the sequential appearance and disappearance of isogenic serotypes in the blood. The only difference between isogenic serotypes is the variable major outer membrane lipoprotein. In the absence of spec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00538-06
更新日期:2006-11-01 00:00:00
abstract::All of the Salmonella enteritidis strains isolated from diseased animals (61 strains) and from beef (2 strains) in Japan and in West Germany (1 strain), except for 2 strains isolated from ducks, harbored either a 36-megadalton (Md) plasmid alone or in combination with several other plasmids of different sizes. It is l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.3.831-833.1985
更新日期:1985-03-01 00:00:00
abstract::Stimulation of the rabbit reticuloendothelial system with viable Mycobacterium bovis (strain BCG), and other agents, had no effect on the development of syphilitic lesions after intradermal or intravenous inoculation with graded doses of Treponema pallidum (virulent Nichol's strain; mean infective doses less than 10)....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.5.1029-1036.1975
更新日期:1975-11-01 00:00:00
abstract::Active immunization with Porphyromonas gingivalis whole-cell preparations has been shown to prevent P. gingivalis infection and oral bone loss. Employing passive antibody transfer and opsonization, we demonstrate with this study that immunization-elicited P. gingivalis-specific immunoglobulin G facilitates clearance o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2408-2411.2004
更新日期:2004-04-01 00:00:00
abstract::Collectins play important roles in host defense against infectious microorganisms. We now demonstrate that the serum collectins mannose-binding lectin (MBL) and conglutinin have less ability to bind to, aggregate, and enhance neutrophil uptake of several strains of gram-negative and gram-positive bacteria than pulmona...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.11.6129-6139.2002
更新日期:2002-11-01 00:00:00
abstract::The genes of the lic1 operon (lic1A to lic1D) are responsible for incorporation of phosphocholine (PCho) into the lipopolysaccharide (LPS) of Haemophilus influenzae. PCho plays a multifaceted role in the commensal and pathogenic lifestyles of a range of mucosal pathogens, including H. influenzae. Structural studies of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00748-07
更新日期:2008-02-01 00:00:00
abstract::Alveolar macrophages are the effector cells largely responsible for clearance of Pneumocystis carinii from the lungs. Binding of organisms to beta-glucan and mannose receptors has been shown to stimulate phagocytosis of the organisms. To further define the mechanisms used by alveolar macrophages for clearance of P. ca...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00393-07
更新日期:2007-08-01 00:00:00