Abstract:
:An inactivated oral enterotoxigenic Escherichia coli (ETEC) vaccine against ETEC diarrhea was given to 25 adult Swedish volunteers. The vaccine consisted of formalin-killed E. coli bacteria expressing the most common colonization factor antigens (CFAs), i.e., CFA/I, -II, and -IV, and recombinantly produced cholera B subunit (CTB). Immunoglobulin A (IgA) antibody responses in intestinal lavage fluid to CTB and CFAs were determined and compared with corresponding responses in stool extracts and serum as well as with IgA antibody-secreting cell (ASC) responses in peripheral blood. Two doses of vaccine induced significant IgA responses to the different CFAs in lavage fluid in 61 to 87% of the vaccinees and in stool in 38 to 81% of them. The most frequent responses were seen against CFA/I. The magnitudes of the antibody responses against CTB and CFA/I in stool correlated significantly (CTB, P < 0.01; CFA/I, P < 0. 05) with those in intestinal lavage. Intestinal lavage responses against CFAs were best reflected by the ASC responses, with the sensitivity of the ASC assay being 80 to 85%, followed by stool (sensitivity of 50 to 88%) and serum antibody (sensitivity of 7 to 65%) analyses. CTB-specific immune responses were seen in >90% of the vaccinees in all assays.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Ahrén C,Jertborn M,Svennerholm AMdoi
10.1128/IAI.66.7.3311-3316.1998subject
Has Abstractpub_date
1998-07-01 00:00:00pages
3311-6issue
7eissn
0019-9567issn
1098-5522journal_volume
66pub_type
杂志文章abstract::Previous studies have indicated that the ability to bind to fibronectin is a key feature in successful cell invasion by Campylobacter jejuni. Given the spatial distribution of fibronectin and the architecture of the epithelium, this suggests the possibility that C. jejuni cell invasion might preferentially occur at th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.12.6665-6671.2002
更新日期:2002-12-01 00:00:00
abstract::Infection with gram-negative monocytotropic Ehrlichia strains results in a fatal toxic shock-like syndrome characterized by a decreased number of Ehrlichia-specific CD4(+) Th1 cells, the expansion of tumor necrosis factor alpha (TNF-alpha)-producing CD8(+) T cells, and the systemic overproduction of interleukin-10 (IL...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00198-07
更新日期:2007-09-01 00:00:00
abstract::Virulence of the fungal pathogen Aspergillus fumigatus is in part based on the saprophytic lifestyle that this mold has evolved. A crucial function for saprophytism resides in secreted proteases that allow assimilation of proteinaceous substrates. The impact of extracellular proteolytic activities on the pathogenesis ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00425-09
更新日期:2009-09-01 00:00:00
abstract::Antigen preparations derived from Plasmodium falciparum-infected erythrocytes (but not from uninfected erythrocytes) can stimulate the in vitro proliferation of peripheral blood lymphocytes from malaria-sensitized as well as nonsensitized donors. The possibility that the nonspecific responses might be due to a parasit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.106-110.1979
更新日期:1979-04-01 00:00:00
abstract::Recent evidence suggests that immune-mediated gastric epithelial cell apoptosis through Fas-Fas ligand interactions participates in Helicobacter pylori disease pathogenesis. To define the role of Fas signaling in vivo, H. pylori strain SS1 infection in C57BL/6 mice was compared to that in mice deficient in the Fas lig...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2591-2597.2002
更新日期:2002-05-01 00:00:00
abstract::Infection of inbred mouse strains with Borrelia burgdorferi results in the development of experimental Lyme arthritis. The degree of arthritic pathology has been suggested to correlate with the level of spirochete burden within tissues. To investigate this further, we infected resistant DBA/2 (DBA) and susceptible C3H...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.5.2065-2071.1998
更新日期:1998-05-01 00:00:00
abstract::We report on the colonization of murine trigeminal ganglia after sequential infection of mice by herpes simplex viruses (HSVs). In preliminary studies, we have established that whereas the HSV-1(F) strain efficiently colonizes ganglia when inoculated by either the ear or eye routes, the HSV-1 X HSV-2 recombinant C7D c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.2.702-708.1983
更新日期:1983-08-01 00:00:00
abstract::Advanced human periodontitis is considered to be a B-cell lesion, but the cellular infiltrate contains several cell types, the distribution of which has not been determined. This experiment was designed to characterize and identify the immunocompetent cells on histological sections and in eluates from diseased human g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.1.365-374.1983
更新日期:1983-07-01 00:00:00
abstract::The Mycobacterium tuberculosis exported repetitive protein (Erp) is a virulence determinant required for growth in cultured macrophages and in vivo. To better understand the role of Erp in Mycobacterium pathogenesis, we generated a mutation in the erp homologue of Mycobacterium marinum, a close genetic relative of M. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02061-05
更新日期:2006-06-01 00:00:00
abstract::Lipoteichoic acids (LTAs) belong to the immunostimulatory class of molecules of gram-positive bacteria (GPB). Previous investigations showed that the macrophage scavenger receptor (SR), a glycosylated trimeric transmembrane protein, binds directly to many GPB, possibly via LTA. SR binding to other ligands is dependent...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3318-3325.1996
更新日期:1996-08-01 00:00:00
abstract::The enzyme-linked immunosorbent assay technique was used to quantitate, in milligrams per milliliter, anti-colonization factor antigen/I (CFA/I) immunoglobulin G (IgG) in acute- and convalescent-phase sera of individuals who experienced diarrhea associated with CFA/I-positive enterotoxigenic Escherichia coli. Purified...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.525-531.1980
更新日期:1980-02-01 00:00:00
abstract::Piglets suckled by dams that had been vaccinated with K88 antigen were significantly more resistant to deaths caused by neonatal diarrhea after challenge with a large dose of a K88-positive enteropathogenic strain of Escherichia coli than piglets suckled by control dams. The factors most likely to be involved in prote...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.667-676.1976
更新日期:1976-03-01 00:00:00
abstract::Antigens were extracted from a virulent isolate of Pasteurella multocida (serotype 3, 12, 15:D) with potassium thiocyanate, and a vaccine was prepared. Pasteurella-free rabbits were vaccinated intranasally and intraconjuctivally twice with a 2-week interval and challenged intranasally with the homologous P. multocida ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.3.498-504.1985
更新日期:1985-09-01 00:00:00
abstract::The Fcγ receptor IIIb (FcγRIIIb) is a low-affinity receptor of IgG and is essential in neutrophil-mediated effector functions. Different allelic forms of FcγRIIIb carrying human neutrophil antigen (HNA-1a, -1b, -1c, and -1d) have been identified. Here, we have generated stable transfected HEK293 cell lines expressing ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00924-17
更新日期:2018-07-23 00:00:00
abstract::Gram-negative organisms incorporate hydroxy fatty acids into the lipid A moiety of lipopolysaccharide (LPS), and in the case of some members of the family Enterobacteriaceae, hydroxy fatty acids are incorporated exclusively into lipid A. However, a limited number of Bacteroides species have been shown to incorporate s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3753-3760.1994
更新日期:1994-09-01 00:00:00
abstract::Both dextransucrase and mutansynthetase activities have been purified from the culture fluids of Streptococcus mutans GS-5 (serotype c). Although homogeneous dextransucrase preparations normally synthesize little insoluble glucan, essentially all of the glucan synthesized by this enzyme in the presence of 1.5 M (NH4)2...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.2.763-770.1983
更新日期:1983-11-01 00:00:00
abstract::The complement system plays a key role in resistance to cryptococcosis. In the present study, we examined several factors that influence the binding of C3 cleavage fragments to Cryptococcus neoformans. Binding of C3 was determined by using normal human serum supplemented with 125I-labeled C3. Incubation of encapsulate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.1-5.1986
更新日期:1986-04-01 00:00:00
abstract::The need for agents designed to modify immune response in the treatment of patients with viral infection, immunodeficiency, or cancer prompted the present study on the mechanisms of action of isoprinosine, a compound developed for antiviral use and whose therapeutic activity may involve the immune system. The effect o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.2.382-387.1976
更新日期:1976-02-01 00:00:00
abstract::The effects of cyclophosphamide (CY) treatment on acute murine cytomegalovirus (MCMV) infection were studied to explore the potential usefulness of MCMV as a means of detecting immune dysfunction and to identify host defense mechanisms important for protection against MCMV. Conditions found optimal for enhancing MCMV ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.3.1046-1055.1982
更新日期:1982-12-01 00:00:00
abstract::Immunoglobulin A1 (IgA1) proteases are produced by a number of different species of bacteria which cause infection at human mucosal surfaces. The sole substrate of these proteases is human IgA1. Cleavage is within the hinge region of IgA1, although there is variability in the exact peptide bond within the hinge region...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.2.320-331.1990
更新日期:1990-02-01 00:00:00
abstract::Clostridium perfringens enterotoxin is the major virulence factor involved in the pathogenesis of C. perfringens type A food poisoning and several non-food-borne human gastrointestinal illnesses. The enterotoxin gene, cpe, is located on the chromosome of food-poisoning isolates but is found on a large plasmid in non-f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.3483-3487.2001
更新日期:2001-05-01 00:00:00
abstract::Patients that have suffered a major injury may sustain a period of immunocompromise and altered Th1/Th2 cytokine balance that can predispose them to opportunistic infections. Pseudomonas aeruginosa is frequently a causative organism for nosocomial infections in critically ill patients and is associated with high morta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.12.6892-6901.2004
更新日期:2004-12-01 00:00:00
abstract::We identified a T-cell determinant of the 35-kDa antigen of Mycobacterium leprae which is discriminatory against cross-sensitization by its closely related homologue in Mycobacterium avium. From synthetic peptides covering the entire sequence, those with the highest affinity and permissive binding to purified HLA-DR m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1501-1504.1999
更新日期:1999-03-01 00:00:00
abstract::Toxicity of lipopolysaccharide (LPS) (endotoxin) is, to a large extent, mediated by the activation of monocytes/macrophages and subsequent release of monokines, such as interleukin-1 (IL-1) and tumor necrosis factor alpha (TNF-alpha). It is known that LPS binds readily to serum lipoproteins and that LPS-lipoprotein co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.7.2237-2245.1989
更新日期:1989-07-01 00:00:00
abstract::A set of strain- and size-variant highly immunogenic membrane surface protein antigens of Mycoplasma bovis, which has been identified by a monoclonal antibody, is shown in this report to make up a family of antigenically and structurally related lipid-modified proteins, designated Vsps (variable surface proteins). By ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.11.5075-5084.1994
更新日期:1994-11-01 00:00:00
abstract::In bacterial infections, mononuclear and polymorphonuclear phagocytes are key components of host defenses. Recent investigations have indicated that chemokines are able to recruit and activate phagocytes. In particular, interleukin-8 (IL-8) attracts polymorphonuclear leukocytes (PMNs), while monocyte chemoattractant p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.4005-4014.1994
更新日期:1994-09-01 00:00:00
abstract::The Bordetella pertussis BrkA protein protects against the bactericidal activity of complement and antibody; however, some individuals mount an immune response that overcomes this bacterial defense. To further characterize this process, the bactericidal activities of sera from 13 adults with different modes of exposur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1424-1431.1999
更新日期:1999-03-01 00:00:00
abstract::Sixty-one patients with a variety of different illnesses were studied with respect to skin test anergy and the presence of serum chemotactic inhibitors. In initial testing, 55% of the patient tests demonstrated negative skin test responses to all six test antigens. Sera from 65% of these anergic patients were capable ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.5.1003-1009.1974
更新日期:1974-11-01 00:00:00
abstract::Mucosa-associated invariant T (MAIT) cells are an innate-like T cell subset in mammals that recognize microbial vitamin B metabolites presented by the evolutionarily conserved major histocompatibility complex class I (MHC I)-related molecule, MR1. Emerging data suggest that MAIT cells may be an attractive target for v...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00524-20
更新日期:2020-12-15 00:00:00
abstract::We assessed cell-mediated immunity to herpes simplex virus type 1 antigen in patients suffering from recurrent cold sores and in a series of healthy controls. Paradoxically, all those subject to recurrent herpetic infections had, without exception, evidence of cell-mediated immunity to herpes antigens. This was demons...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.2.355-359.1975
更新日期:1975-02-01 00:00:00