Abstract:
:Genetically based natural resistance to brucellosis in cattle provides for novel strategies to control zoonotic diseases. Bovine NRAMP1, the homologue of a murine gene (Bcg), has been identified as a major candidate for controlling the in vivo resistant phenotype. We developed an in vitro model for expression of resistance- and susceptibility-associated alleles of bovine NRAMP1 as stable transgenes under the regulatory control of the bovine NRAMP1 promoter in the murine RAW264.7 macrophage cell line (Bcg(s)) to analyze the regulation of the NRAMP1 gene and its role in macrophage function. We demonstrated that the 5'-flanking region of bovine NRAMP1, despite the lack of TATA and CAAT boxes, has a functional promoter capable of driving the expression of a transgene in murine macrophages. A polymorphism within a microsatellite in the 3' untranslated region critically affects the expression of bovine NRAMP1 and the control of in vitro replication of Brucella abortus but not Salmonella enterica serovar Dublin. We did not observe any differences in the production of NO by resting or gamma interferon (IFN-gamma)- and IFN-gamma-lipopolysaccharide (LPS)-treated transfected cell lines, yet the resistant transfected cell lines produced significantly less NO than other cell lines, following stimulation with LPS at 24 and 48 h.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Barthel R,Feng J,Piedrahita JA,McMurray DN,Templeton JW,Adams LGdoi
10.1128/IAI.69.5.3110-3119.2001keywords:
subject
Has Abstractpub_date
2001-05-01 00:00:00pages
3110-9issue
5eissn
0019-9567issn
1098-5522journal_volume
69pub_type
杂志文章abstract::In this study, cytokine patterns produced by CD4(+) T cells isolated from antrum or corpus gastral biopsy specimens of 10 patients with Helicobacter pylori-positive gastritis were compared. To this end, expression of intracellular cytokines (interleukin-4 [IL-4] and gamma interferon) and of CD4 was assessed by flow cy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5543-5546.1998
更新日期:1998-11-01 00:00:00
abstract::Individuals with sickle cell anemia are subject to serious infections caused by a number of bacteria, including Salmonella species and Staphylococcus aureus. It has been suggested that in sickle cell anemia, extensive erythrophagocytosis by macrophages may interfere with their antibacterial function and thereby predis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.3.917-923.1983
更新日期:1983-06-01 00:00:00
abstract::Borrelia burgdorferi synthesizes a variety of differentially regulated outer surface lipoproteins in the tick vector and in vertebrate hosts. Among these is OspD, a protein that is highly induced in vitro by conditions that mimic the tick environment. Using genetically engineered strains in which ospD is deleted, we d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00714-07
更新日期:2008-05-01 00:00:00
abstract::Rhodococcus equi is a facultative intracellular pathogen of macrophages, relying on the presence of a conjugative virulence plasmid harboring a 21-kb pathogenicity island (PAI) for growth in host macrophages. The PAI encodes a family of 6 virulence-associated proteins (Vaps) in addition to 20 other proteins. The contr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00230-15
更新日期:2015-08-01 00:00:00
abstract::Inbred CBA/J mice were used in developing a defined in vivo model for studying host-parasite relationships in cryptococcosis. Mice were infected either intranasally or intraperitoneally with 10(3) viable Cryptococcus neoformans cells. At weekly intervals over a 92-day period, C. neoformans growth profiles in the lungs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Chlamydia trachomatis is a strict human pathogen producing infections that cause medically important chronic inflammatory diseases, such as blinding trachoma and tubal factor infertility. Isolates exist as serotypes that fall into distinct biologic and pathological groups corresponding to differences in infection tiss...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.12.7063-7072.2004
更新日期:2004-12-01 00:00:00
abstract::Human opsonins directed against specific meningococcal outer membrane structures in sera obtained during meningococcal disease were quantified with a recently developed antigen-specific, opsonin-dependent phagocytosis and oxidative burst assay. Outer membrane vesicles (OMVs) and PorA (class 1) and PorB (class 3) prote...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.5.2552-2560.1999
更新日期:1999-05-01 00:00:00
abstract::This study compared infection and transformation of peripheral blood mononuclear cells (PBM) of Boran cattle and African buffalo in vitro to determine whether differences occurred which could account for the greater susceptibility of Boran cattle to infection with Theileria parva subsp. parva and T. parva subsp. lawre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.53.1.186-191.1986
更新日期:1986-07-01 00:00:00
abstract::Stenotrophomonas maltophilia is a ubiquitous bacterium and an emerging nosocomial pathogen. This bacterium is resistant to many antibiotics, associated with a number of infections, and a significant health risk, especially for immunocompromised patients. Given that Caenorhabditis elegans shares many conserved genetic ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00711-15
更新日期:2015-12-07 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00172-20
更新日期:2020-10-19 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00542-15
更新日期:2015-10-01 00:00:00
abstract::A total of 32 patients with Plasmodium falciparum malaria were studied. Of these, 23 had benign infections, and 9 had typical cerebral malaria. Cryoglobulins, circulating immune complexes detected by a C1q-binding assay, and hypocomplementemia were found in eight of nine patients with cerebral malaria. Raised levels o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.530-535.1981
更新日期:1981-02-01 00:00:00
abstract::The enzymes responsible for reducing H2O2 were surveyed in 49 strains of Leptospira by using semiquantitative assays for catalase and peroxidase. The survey revealed a differential distribution of catalase and peroxidase activities between the two leptospiral complexes. The pathogenic Leptospira interrogans strains ga...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.3.672-675.1978
更新日期:1978-12-01 00:00:00
abstract::Rabbit-specific enteropathogenic Escherichia coli (REPEC) is an attaching and effacing pathogen of young rabbits. Using signature-tagged mutagenesis, we identified several known colonization factors of REPEC as well as a gene predicted to encode a novel autotransporter protein. This novel gene was termed rpeA for REPE...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00972-06
更新日期:2007-09-01 00:00:00
abstract::When spleen cells derived from C57BL/6 mice immunized with L cells 7 days previously were cocultured with antigenic cells, immune interferon appeared in the culture fluid. We analyzed the tissue distribution of the immune interferon-producing cells (IIPC) which appeared in various lymphoid organs after allogeneic stim...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-08-01 00:00:00
abstract::Syphilis has been recognized as a disease since the late 1400s, yet there is no practical vaccine available. One impediment to the development of a vaccine is the lack of understanding of multiple reinfections in humans despite the development of robust immune responses during the first episode. It has been shown that...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.10.5605-5612.2003
更新日期:2003-10-01 00:00:00
abstract::In experimental visceral leishmaniasis, in which the tissue macrophage is the target, in vivo responsiveness to conventional chemotherapy (pentavalent antimony [Sb]) requires a T-cell-dependent mechanism. To determine if this mechanism involves gamma interferon (IFN-gamma)-induced activation and/or specific IFN-gamma-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.1.288-293.2000
更新日期:2000-01-01 00:00:00
abstract::The distribution of dengue-2 antigens was studied in infected monkey kidney cells (LLC MK2) using an indirect, horseradish peroxidase-conjugated immunoglobulin technique. This procedure allowed both light and electron microscopic examination of serial-step sections of individual cells cut in a plane perpendicular to t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.6.1699-1709.1976
更新日期:1976-06-01 00:00:00
abstract::Fifteen clinical isolates of Trichomonas vaginalis were evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with transfer to nitrocellulose and by immunoblots probed with human sera. All T. vaginalis isolates showed similar banding patterns by Coomassie brilliant blue and silver staining of the elec...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.1.250-253.1986
更新日期:1986-01-01 00:00:00
abstract::The tetrasaccharide 3-deoxy-alpha-D-manno-2-octulosonic acid (alpha-KDO) (2----8)-alpha-KDO(2----4)-alpha-KDO(2----6)-beta GlcNAc, a partial structure of chlamydial lipopolysaccharide (LPS) representing a genus-specific epitope, was synthesized and covalently linked to bovine serum albumin, resulting in an artificial ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.4.1314-1321.1992
更新日期:1992-04-01 00:00:00
abstract::Smoking is associated with increased susceptibility to tuberculosis and influenza. However, little information is available on the mechanisms underlying this increased susceptibility. Mice were left unexposed or were exposed to cigarette smoke and then infected with Mycobacterium tuberculosis by aerosol or influenza A...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00709-10
更新日期:2011-01-01 00:00:00
abstract::A cytotoxigenic Clostridium difficile strain that fails to produce toxin A but causes hemorrhage and bloody fluid accumulation in ligated ileal loops of rabbits and hemorrhage and diarrhea in hamsters is described. The lack of reaction of DNA from this strain in hybridization studies with a toxin A gene-specific 4.5-k...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.10.4192-4199.1992
更新日期:1992-10-01 00:00:00
abstract::In order to further characterize human host defenses against Coccidioides immitis, extracts of this fungus were tested in vitro for their ability to attract polymorphonuclear leukocytes derived from peripheral blood of uninfected subjects. Soluble substances prepared from the mycelial (saprophytic) and spherule (tissu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.3.862-865.1978
更新日期:1978-09-01 00:00:00
abstract::Salmonella enterica subspecies I serovars are common bacterial pathogens causing diseases ranging from enterocolitis to systemic infections. Some serovars are adapted to specific hosts, whereas others have a broad host range. The molecular mechanisms defining the virulence characteristics and the host range of a given...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.1.632-644.2006
更新日期:2006-01-01 00:00:00
abstract::Cytokine gene expression in cells migrating in afferent and efferent intestinal lymph was monitored for extended time periods in individual sheep experimentally infected with the nematode Trichostrongylus colubriformis. Animals from stable selection lines with increased levels of either genetic resistance (R) or susce...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.4.2175-2183.2005
更新日期:2005-04-01 00:00:00
abstract::No effective therapy exists for Cryptosporidium parvum, a coccidial protozoan parasite that causes severe diarrhea in patients with AIDS. The role of microtubules in parasite invasion of host cells was investigated by incubating 10(7) oocysts with a HT 29.74 cell line for 24 h in the presence of microtubule-disrupting...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4888-4890.1993
更新日期:1993-11-01 00:00:00
abstract::The pH 6 antigen (Psa) of Yersinia pestis consists of fimbriae with adhesive properties of potential importance for the pathogenesis of plague, including pneumonic plague. The Psa fimbriae mediate bacterial binding to human alveolar epithelial cells. The Psa fimbriae bound mostly to one component present in the total ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01153-06
更新日期:2007-03-01 00:00:00
abstract::A nasal vaccine, consisting of outer membrane vesicles (OMVs) from group B Neisseria meningitidis, was given to 12 volunteers in the form of nose drops or nasal spray four times at weekly intervals, with a fifth dose 5 months later. Each nasal dose consisted of 250 microg of protein, equivalent to 10 times the intramu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1334-1341.1998
更新日期:1998-04-01 00:00:00
abstract::In this study, we report on a novel approach based on modification of the intestinal surface to prevent diarrhea caused by enterotoxigenic Escherichia coli (ETEC). The removable intestinal tie adult rabbit diarrhea (RITARD) model was used to test the efficacy of an enteric-coated protease preparation (Detach; Enzacor ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3708-3714.1991
更新日期:1991-10-01 00:00:00
abstract::The chemotactic behavior of Campylobacter jejuni was determined in the presence of different amino acids, carbohydrates, organic acids, and preparations and constituents of mucin and bile. L-Fucose was the only carbohydrate and L-aspartate, L-cysteine, L-glutamate, and L-serine were the only amino acids producing a ch...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.6.1560-1566.1988
更新日期:1988-06-01 00:00:00