Abstract:
:Fifteen clinical isolates of Trichomonas vaginalis were evaluated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis with transfer to nitrocellulose and by immunoblots probed with human sera. All T. vaginalis isolates showed similar banding patterns by Coomassie brilliant blue and silver staining of the electrophoresis gels and by amido black staining of the nitrocellulose. However, by the immunoblot technique, differences in banding patterns were noted, particularly in the high-molecular-weight zone (greater than 1.5 X 10(5], which were consistent in numerous experiments. A common immunogenic band was noted at a molecular weight of approximately 100,000 in all T. vaginalis isolates probed with six sera reactive in an enzyme-linked immunosorbent assay but was not seen or was only faintly visible when isolates were probed with sera considered to be nonreactive by the assay. Many other bands were identified, some of which appeared common to all isolates, but were not recognized by all sera tested. These studies demonstrate the antigenic heterogeneity of T. vaginalis and show that different individuals appear to respond immunologically to different T. vaginalis antigens.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Garber GE,Proctor EM,Bowie WRdoi
10.1128/IAI.51.1.250-253.1986subject
Has Abstractpub_date
1986-01-01 00:00:00pages
250-3issue
1eissn
0019-9567issn
1098-5522journal_volume
51pub_type
杂志文章abstract::During a Plasmodium chabaudi infection in interleukin-10 (IL-10) knockout mice, there is greater parasite sequestration, more severe cerebral edema, and a high frequency of cerebral hemorrhage compared with infection of C57BL/6 mice. Anti-tumor necrosis factor alpha treatment ameliorated both cerebral edema and hemorr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.5.3054-3058.2004
更新日期:2004-05-01 00:00:00
abstract::Insertional mutagenesis studies of mrpB, a putative pilin-encoding open reading frame of the mrp gene cluster, which encodes mannose-resistant Proteus-like (MR/P) fimbriae of Proteus mirabilis, indicate that MrpB functions as the terminator for fimbrial assembly. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1759-1763.1998
更新日期:1998-04-01 00:00:00
abstract::It is not clear how the host initially recognizes and responds to infection by gram-negative pathogenic Brucella spp. It was previously shown (D. S. Weiss, B. Raupach, K. Takeda, S. Akira, and A. Zychlinsky, J. Immunol. 172:4463-4469, 2004) that the early macrophage response against gram-negative bacteria is mediated ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.5137-5143.2005
更新日期:2005-08-01 00:00:00
abstract::The initial encounter of Leishmania cells and cells from the immune system is fundamentally important in the outcome of infection and determines disease development or resistance. We evaluated the anti-Leishmania amazonensis response of naive volunteers by using an in vitro priming (IVP) system and comparing the respo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7453-7460.2001
更新日期:2001-12-01 00:00:00
abstract::The cellular source of interleukin-6 (IL-6) during infection of mice with Listeria monocytogenes was investigated both in vitro and in vivo. Peritoneal cells taken at intervals from infected mice and cultured in vitro without added stimulus produced high titers of IL-6 peaking 2 days postinfection in a time course sim...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2626-2631.1993
更新日期:1993-06-01 00:00:00
abstract::The leading malaria vaccine candidate, RTS,S, based on the Plasmodium falciparum circumsporozoite protein (CSP), will likely be the first publicly adopted malaria vaccine. However, this and other subunit vaccines, such as virus-vectored thrombospondin-related adhesive protein (TRAP), provide only intermediate to low l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01063-15
更新日期:2015-12-14 00:00:00
abstract::Lipopolysaccharide (LPS) is an amphipathic macromolecule that is highly aggregated in aqueous preparations. LPS-binding protein (LBP) catalyzes the transfer of single LPS molecules, segregated from an LPS aggregate, to high-density lipoproteins (HDL), which results in the neutralization of LPS. When fluorescein isothi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.9.4954-4960.2000
更新日期:2000-09-01 00:00:00
abstract::Salmonellae possess the ability to adhere to and invade macrophages and in so doing trigger a number of intracellular events that are associated with cellular activation. As an initial approach to defining the mechanisms by which invasive salmonellae alter macrophage function, we have explored the impact of Salmonella...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.5.1820-1826.1995
更新日期:1995-05-01 00:00:00
abstract::Human plasma was bactericidal for small numbers of Klebsiella pneumoniae, but larger numbers grew slowly over a period of 24 h. Human polymorphs in a clot of autologous plasma had a bacteriostatic effect on relatively large numbers of bacteria for up to 24 h and were much more effective than plasma alone. The bacteric...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.3.723-730.1986
更新日期:1986-03-01 00:00:00
abstract::Preliminary studies established methods for obtaining maximum yields of viable cells. Liquid shaken cultures of Histoplasma capsulatum provided a maximum of 4 x 10(8) to 5 x 10(8) cells/ml regardless of the inoculum size. Under optimum conditions, cells were viable (90 to 95%) for 168 to 240 hr. Generation times range...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.2.116-125.1971
更新日期:1971-08-01 00:00:00
abstract::Salivary proline-rich proteins (PRPs), which were purified from parotid saliva, were adsorbed onto 15-nm-diameter gold particles to visualize specific binding of the salivary molecules to Actinomyces viscosus type 1 fimbriae. Negatively stained preparations incubated with PRP-gold conjugates but not bovine serum album...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1986-1991.1990
更新日期:1990-06-01 00:00:00
abstract::Cyclic dimeric GMP (c-di-GMP), a bacterial second messenger, is known to regulate bacterial biofilm and sessility. Replication of an obligatory intracellular pathogen, Ehrlichia chaffeensis, is characterized by formation of bacterial aggregates called morulae inside membrane-bound inclusions. When E. chaffeensis matur...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05320-11
更新日期:2011-10-01 00:00:00
abstract::The abilities of six strains of Mycobacterium bovis strain BCG to replicate in vivo and to cause spleen enlargement in the CDF(1) male mouse were compared. The strains were grown in synthetic medium and harvested soon after complete pellicles were produced on liquid medium. In addition, two freeze-dried commercially a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.5.736-742.1973
更新日期:1973-11-01 00:00:00
abstract::Staphylococcus aureus fibronectin-binding proteins (FnBPs) play a critical role in S. aureus pathogenesis. FnBPs mediate adhesion to fibronectin and invasion of mammalian cells, including epithelial, endothelial, and fibroblastic cells, by fibronectin bridging to the host cell fibronectin receptor integrin (alpha(5))b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.12.7155-7163.2004
更新日期:2004-12-01 00:00:00
abstract::Chlamydia trachomatis serovar L2 is an obligate intracellular bacterium which is internalized in target epithelial cells by endocytosis and resides within a membrane-bound vesicle. Over the next several hours following entry, individual serovar L2-containing vesicles fuse with one another to form a single membrane-bou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.4.1208-1214.1996
更新日期:1996-04-01 00:00:00
abstract::To demonstrate local synthesis of anti-Mycobacterium leprae antibodies, biopsies from skin lesions of leprosy patients were cultured in vitro in a medium containing 14C-labeled lysine and isoleucine, and the culture fluids were analyzed by crossed immunoelectrophoresis with intermediate gel and autoradiography. The re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.297-301.1980
更新日期:1980-02-01 00:00:00
abstract::Salmonella enterica serovars Typhimurium and Dublin lysed primary bovine alveolar macrophages and immortalized J774.2 macrophage-like cells in the absence of either the morphological changes or DNA fragmentation characteristic of apoptosis. Macrophage lysis was dependent on a subset of caspases and an intact sipB gene...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.6.3744-3747.2000
更新日期:2000-06-01 00:00:00
abstract::Bovine NK cells have recently been characterized and the present study describes the interaction between NK cells, antigen-presenting cells, and secreted mycobacterial proteins. Gamma interferon (IFN-gamma) production by NK cells was seen in approximately 30% of noninfected calves in response to the Mycobacterium tube...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.9.5628-5635.2005
更新日期:2005-09-01 00:00:00
abstract::An asd-stabilized plasmid carrying enterotoxigenic Escherichia coli cfaABCE genes was constructed and called pJGX15C-asd+. Expression of colonization factor antigen I (CFA/I) by this plasmid occurs independently of the cfaABCE positive regulator cfaR in attenuated Salmonella delta aro delta asd strain H683 and nonpath...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.12.4933-4938.1995
更新日期:1995-12-01 00:00:00
abstract::Brucella abortus is recognized by several Toll-like receptor (TLR)-associated pathways triggering proinflammatory responses that affect both the nature and intensity of the immune response. Previously, we demonstrated that B. abortus-mediated dendritic cell (DC) maturation and control of infection are dependent on the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01356-12
更新日期:2013-05-01 00:00:00
abstract::Thymidine kinase (TK) from herpes simplex virus type 1 (HSV-1) biochemically transformed HeLa cells, purified by affinity chromatography, has been characterized with respect to its electrophoretic mobility, molecular weight, activation energy, substrate specificity, and immunological specificity. TK purified from HSV-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.2.486-492.1977
更新日期:1977-05-01 00:00:00
abstract::Streptolysin-O (SLO) is a thiol-activated, membrane-damaging protein toxin of Mr 69,000 that is produced by most strains of beta-hemolytic group A streptococci. Native, primarily water-soluble toxin molecules bind to cholesterol-containing target membranes to assemble into supramolecular curved rod structures (25 to 1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.1.52-60.1985
更新日期:1985-01-01 00:00:00
abstract::A DNA microarray containing fragments of 137 Borrelia burgdorferi B31 putative lipoprotein genes was used to examine Lyme disease spirochetes. DNA from B. burgdorferi sensu stricto B31, 297, and N40; Borrelia garinii IP90; and Borrelia afzelii P/Gau was fluorescently labeled and hybridized to the microarray, demonstra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.3300-3303.2002
更新日期:2002-06-01 00:00:00
abstract::Pseudomonas aeruginosa is considered an extracellular pathogen. Using assays to determine intracellular survival in the presence of gentamicin, we have demonstrated that some strains of P. aeruginosa are able to invade corneal cells during experimental bacterial keratitis in mice. Although intracellular bacteria were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3485-3493.1994
更新日期:1994-08-01 00:00:00
abstract::Alternate modalities for the treatment of Mycobacterium tuberculosis are needed due to the rise in numbers of immunosuppressed individuals at risk for serious disease and the increasing prevalence of multidrug-resistant isolates. Interleukin-12 (IL-12) has been shown to improve immune responses against M. tuberculosis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.5.2976-2988.2004
更新日期:2004-05-01 00:00:00
abstract::The short-term kinetics of fluoride uptake by cells from 20- to 22-h cultures of Streptococcus mutans strain 6715 were studied using rapid filtration and centrifugation techniques. Saline-suspended organisms were diluted with fluoride-containing solutions buffered at four different pH values (2.0, 4.0, 5.5, and 8.2). ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.3.680-687.1977
更新日期:1977-12-01 00:00:00
abstract::Bactericidal/permeability-increasing protein (BPI), a human neutrophil granule protein, has been shown to bind lipopolysaccharide (LPS) and neutralize LPS-mediated cytokine production in adherent monocytes and the whole-blood system. In this study we investigated the ability of recombinant human BPI (rBPI) to inhibit ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3930-3936.1994
更新日期:1994-09-01 00:00:00
abstract::In the lungs of mice vaccinated with Mycobacterium bovis BCG, there was an accumulation of CD4 cells expressing the activated effector phenotype CD44hi CD62 ligandlo) (CD62Llo) which were capable of secreting gamma interferon. Upon cell transfer, however, cells expressing a resting/naïve phenotype (CD44lo CD62Lhi) wer...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7759-7764.2005
更新日期:2005-11-01 00:00:00
abstract::Serum antibody responses to toxic shock syndrome (TSS) toxin 1 (TSST-1) and staphylococcal enterotoxins A, B, and C were determined by western blot (immunoblot) analysis of acute- and convalescent-phase paired sera from 18 TSS- and 31 non-TSS-associated Staphylococcus aureus infections. Compared with non-TSS cases, se...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.1.231-234.1989
更新日期:1989-01-01 00:00:00
abstract::Peptide 9 (TDAYNQKLSERRAN) and peptide 10 (NATAEGRAINRRVE) represent surface-exposed epitopes of outer membrane protein F of Pseudomonas aeruginosa. Rats immunized with four intramuscular inoculations on days 0, 14, 28, and 42 with either peptide 9 or peptide 10 conjugated to keyhole limpet hemocyanin were afforded pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.6.2347-2351.1995
更新日期:1995-06-01 00:00:00