Abstract:
:Bovine NK cells have recently been characterized and the present study describes the interaction between NK cells, antigen-presenting cells, and secreted mycobacterial proteins. Gamma interferon (IFN-gamma) production by NK cells was seen in approximately 30% of noninfected calves in response to the Mycobacterium tuberculosis complex-specific protein ESAT-6, MPP14 from Mycobacterium avium subsp. paratuberculosis, and purified protein derivative (PPD) from M. tuberculosis. In contrast, no response was induced by MPB70, which is another M. tuberculosis complex-specific secreted antigen. The production of IFN-gamma by NK cells in whole blood in response to ESAT-6 and MPP14 was demonstrated using intracellular staining together with surface labeling for the NK cell-specific receptor, NKp46, or CD3. Furthermore, the depletion of NK cells from peripheral blood mononuclear cells completely abolished the IFN-gamma production. The response was mediated through stimulation of adherent cells and was largely independent of contact between adherent cells and the NK cells. Neutralization of interleukin-12 only partly inhibited IFN-gamma production, showing that other cytokines were also involved. The demonstration of NK cell-mediated IFN-gamma production in young cattle provides an explanation for the nonspecific IFN-gamma response frequently encountered in young cattle when using the IFN-gamma test in diagnosis of mycobacterial infections.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Olsen I,Boysen P,Kulberg S,Hope JC,Jungersen G,Storset AKdoi
10.1128/IAI.73.9.5628-5635.2005keywords:
subject
Has Abstractpub_date
2005-09-01 00:00:00pages
5628-35issue
9eissn
0019-9567issn
1098-5522pii
73/9/5628journal_volume
73pub_type
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pub_type: 杂志文章
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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