Abstract:
:Chlamydia trachomatis serovar L2 is an obligate intracellular bacterium which is internalized in target epithelial cells by endocytosis and resides within a membrane-bound vesicle. Over the next several hours following entry, individual serovar L2-containing vesicles fuse with one another to form a single membrane-bound vesicle (or inclusion) within which the microcolony develops. The experiments reported here directly examined the pH of vesicles containing chlamydiae. The pH was determined by measuring emission ratios of the fluorescent, pH-sensitive probe SNAFL (5-[and 6-]-carboxyseminaphthofluorescein-1, succinimidyl ester) conjugated to chlamydiae. The pH remained above 6.0 at 2, 4, and 12 h after infection, while the pH of vesicles contained heat-killed organisms fell 5.3. In the presence of amines, which raise the pH of acidic compartments, C. trachomatis inclusion formation was unaffected. Inactivation of Na+,K+ -ATPases, the ion pumps responsible for maintaining a pH above 6 within early endocytic vesicles, inhibited the growth of C. trachomatis within epithelial cells. Preventing vesicular acidification by inhibiting the vacuolar proton ATPase did not affect chlamydial growth. Thus, chlamydiae do not reside within highly acidic vesicles and avoid the pathway leading to lysosomes.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Schramm N,Bagnell CR,Wyrick PBdoi
10.1128/IAI.64.4.1208-1214.1996subject
Has Abstractpub_date
1996-04-01 00:00:00pages
1208-14issue
4eissn
0019-9567issn
1098-5522journal_volume
64pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.7.2.226-230.1973
更新日期:1973-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.6.2120-2125.1995
更新日期:1995-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.12.5267-5282.1992
更新日期:1992-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.7.1558-1563.1987
更新日期:1987-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.51.1.250-253.1986
更新日期:1986-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.483-487.1985
更新日期:1985-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.00778-15
更新日期:2015-11-01 00:00:00
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journal_title:Infection and immunity
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doi:
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更新日期:2006-12-01 00:00:00
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doi:10.1128/IAI.51.1.204-208.1986
更新日期:1986-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.2.442-448.1985
更新日期:1985-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.223-227.1981
更新日期:1981-01-01 00:00:00
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doi:10.1128/IAI.19.3.785-791.1978
更新日期:1978-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00711-15
更新日期:2015-12-07 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2017-04-21 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.73.7.4414-4419.2005
更新日期:2005-07-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2004-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4483-4490.1992
更新日期:1992-11-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1991-07-01 00:00:00