Vesicles containing Chlamydia trachomatis serovar L2 remain above pH 6 within HEC-1B cells.


:Chlamydia trachomatis serovar L2 is an obligate intracellular bacterium which is internalized in target epithelial cells by endocytosis and resides within a membrane-bound vesicle. Over the next several hours following entry, individual serovar L2-containing vesicles fuse with one another to form a single membrane-bound vesicle (or inclusion) within which the microcolony develops. The experiments reported here directly examined the pH of vesicles containing chlamydiae. The pH was determined by measuring emission ratios of the fluorescent, pH-sensitive probe SNAFL (5-[and 6-]-carboxyseminaphthofluorescein-1, succinimidyl ester) conjugated to chlamydiae. The pH remained above 6.0 at 2, 4, and 12 h after infection, while the pH of vesicles contained heat-killed organisms fell 5.3. In the presence of amines, which raise the pH of acidic compartments, C. trachomatis inclusion formation was unaffected. Inactivation of Na+,K+ -ATPases, the ion pumps responsible for maintaining a pH above 6 within early endocytic vesicles, inhibited the growth of C. trachomatis within epithelial cells. Preventing vesicular acidification by inhibiting the vacuolar proton ATPase did not affect chlamydial growth. Thus, chlamydiae do not reside within highly acidic vesicles and avoid the pathway leading to lysosomes.


Infect Immun


Infection and immunity


Schramm N,Bagnell CR,Wyrick PB




Has Abstract


1996-04-01 00:00:00












  • Differential priming effects of proinflammatory cytokines on human neutrophil oxidative burst in response to bacterial N-formyl peptides.

    abstract::Cytokines such as tumor necrosis factor alpha (TNF-alpha), granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin 8 (IL-8), IL-6, IL-1 alpha, and IL-1 beta produced during the immune and inflammatory responses to bacterial stimuli have been reported to interact with polymorphonuclear neutrophil (PMN) a...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Elbim C,Bailly S,Chollet-Martin S,Hakim J,Gougerot-Pocidalo MA

    更新日期:1994-06-01 00:00:00

  • Murine malaria: immune complexes inhibit Fc receptor-mediated phagocytosis.

    abstract::Immune complexes have been partially purified from the serum of Plasmodium berghei-infected mice by ultracentrifugation on 10 to 40% linear sucrose gradients, by precipitation with polyethylene glycol, and by gel filtration through Sephacryl S-300. The complexes contain gamma 1, gamma 2a, gamma 2b, and gamma 3 subclas...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Shear HL

    更新日期:1984-04-01 00:00:00

  • Acquired immunity in experimental murine aspergillosis is mediated by macrophages.

    abstract::A number of studies have substantiated the pivotal role of innate defense mechanisms in protection against invasive aspergillosis. However, experiments demonstrating increased resistance to lethal intravenous (i.v.) infection with Aspergillus fumigatus conidia in cortisone-treated or untreated mice preinfected with a ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: de Repentigny L,Petitbois S,Boushira M,Michaliszyn E,Sénéchal S,Gendron N,Montplaisir S

    更新日期:1993-09-01 00:00:00

  • Opsonization modulates Rac-1 activation during cell entry by Leishmania amazonensis.

    abstract::Lesions caused by Leishmania amazonensis normally heal, but relapses occur due to parasite persistence in host tissues. It has been proposed that infection of fibroblasts plays an important role in this process by providing the parasites with a safe haven in which to replicate. However, most previous studies have focu...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Morehead J,Coppens I,Andrews NW

    更新日期:2002-08-01 00:00:00

  • Role of adult worm antigen-specific immunoglobulin E in acquired immunity to Schistosoma mansoni infection in baboons.

    abstract::Allergic-type immune responses, particularly immunoglobulin E (IgE), correlate with protective immunity in human schistosomiasis. To better understand the mechanisms of parasite elimination we examined the immune correlates of protection in baboons (Papio cynocephalus anubis), which are natural hosts for Schistosoma m...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Nyindo M,Kariuki TM,Mola PW,Farah IO,Elson L,Blanton RE,King CL

    更新日期:1999-02-01 00:00:00

  • Antibacterial activity of bladder surface mucin duplicated in the rabbit bladder by exogenous glycosaminoglycan (sodium pentosanpolysulfate).

    abstract::Previous data from our laboratory suggest that the transitional epithelium of the urinary bladder secretes and binds to its surface a glycosaminoglycan. The presence of this substance at the bladder surface markedly reduces the ability of microorganisms to adhere to the mucosa. Furthermore, this glycosaminoglycan can ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Parsons CL,Pollen JJ,Anwar H,Stauffer C,Schmidt JD

    更新日期:1980-03-01 00:00:00

  • Reactive oxygen species-triggered trophoblast apoptosis is initiated by endoplasmic reticulum stress via activation of caspase-12, CHOP, and the JNK pathway in Toxoplasma gondii infection in mice.

    abstract::Toxoplasma gondii infection in pregnant women may result in abortion or in fetal teratogenesis; however, the underlying mechanisms are still unclear. In this paper, based on a murine model, we showed that maternal infection with RH strain T. gondii tachyzoites induced elevated production of reactive oxygen species (RO...

    journal_title:Infection and immunity

    pub_type: 杂志文章,收录出版


    authors: Xu X,Liu T,Zhang A,Huo X,Luo Q,Chen Z,Yu L,Li Q,Liu L,Lun ZR,Shen J

    更新日期:2012-06-01 00:00:00

  • espC pathogenicity island of enteropathogenic Escherichia coli encodes an enterotoxin.

    abstract::At least five proteins are secreted extracellularly by enteropathogenic Escherichia coli (EPEC), a leading cause of infant diarrhea in developing countries. However only one, EspC, is known to be secreted independently of the type III secretion apparatus encoded by genes located within the 35.6-kb locus of enterocyte ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Mellies JL,Navarro-Garcia F,Okeke I,Frederickson J,Nataro JP,Kaper JB

    更新日期:2001-01-01 00:00:00

  • Pathology affects different organs in two mouse strains chronically infected by a Trypanosoma cruzi clone: a model for genetic studies of Chagas' disease.

    abstract::Chagas' disease is a chronic infection caused by Trypanosoma cruzi and represents an important public health burden in Latin America. Frequently the disease evolves undetectable for decades, while in a significant fraction of the affected individuals it culminates in death by heart failure. Here, we describe a novel m...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Marinho CR,Bucci DZ,Dagli ML,Bastos KR,Grisotto MG,Sardinha LR,Baptista CR,Gonçalves CP,Lima MR,Alvarez JM

    更新日期:2004-04-01 00:00:00

  • Antimycobacterial calixarenes enhance innate defense mechanisms in murine macrophages and induce control of Mycobacterium tuberculosis infection in mice.

    abstract::Tuberculosis remains the leading cause of death among infectious diseases, accounting for more than two million deaths annually. The incidence of the disease is increasing globally, partially because of the resurgence of drug-resistant strains of Mycobacterium tuberculosis. Calixarenes are macrocyclic oligomers, some ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Colston MJ,Hailes HC,Stavropoulos E,Hervé AC,Hervé G,Goodworth KJ,Hill AM,Jenner P,Hart PD,Tascon RE

    更新日期:2004-11-01 00:00:00

  • Impact of M49, Mrp, Enn, and C5a peptidase proteins on colonization of the mouse oral mucosa by Streptococcus pyogenes.

    abstract::Resistance to phagocytosis is a hallmark of virulent Streptococcus pyogenes (group A streptococcus). Surface-bound C5a peptidase reduces recruitment of phagocytes to the site of infection, and hyaluronic acid capsules and/or the M protein limit the uptake of streptococci. In this study the relative impact of M and M-l...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ji Y,Schnitzler N,DeMaster E,Cleary P

    更新日期:1998-11-01 00:00:00

  • Apple flavonoid phloretin inhibits Escherichia coli O157:H7 biofilm formation and ameliorates colon inflammation in rats.

    abstract::Pathogenic biofilms have been associated with persistent infections due to their high resistance to antimicrobial agents, while commensal biofilms often fortify the host's immune system. Hence, controlling biofilm formation of both pathogenic bacteria and commensal bacteria is important in bacterium-related diseases. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lee JH,Regmi SC,Kim JA,Cho MH,Yun H,Lee CS,Lee J

    更新日期:2011-12-01 00:00:00

  • Mucosal immunization with an unadjuvanted vaccine that targets Streptococcus pneumoniae PspA to human Fcγ receptor type I protects against pneumococcal infection through complement- and lactoferrin-mediated bactericidal activity.

    abstract::Targeting an antigen to Fc receptors (FcR) can enhance the immune response to the antigen in the absence of adjuvant. Furthermore, we recently demonstrated that intranasal immunization with an FcγR-targeted antigen enhances protection against a category A intracellular mucosal pathogen, Francisella tularensis. To dete...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Bitsaktsis C,Iglesias BV,Li Y,Colino J,Snapper CM,Hollingshead SK,Pham G,Gosselin DR,Gosselin EJ

    更新日期:2012-03-01 00:00:00

  • Macrophage-enhanced germination of Bacillus anthracis endospores requires gerS.

    abstract::Germination of Bacillus anthracis Sterne and plasmidless Delta-Sterne endospores was dramatically enhanced in RAW264.7 macrophage-like cells, while germination of nonpathogenic Bacillus endospores was not. Elimination of gerS, a germinant receptor locus, caused a complete loss of cell-enhanced germination, implicating...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ireland JA,Hanna PC

    更新日期:2002-10-01 00:00:00

  • Legionella pneumophila htpAB heat shock operon: nucleotide sequence and expression of the 60-kilodalton antigen in L. pneumophila-infected HeLa cells.

    abstract::A 60-kilodalton (kDa) immunodominant antigen of Legionella pneumophila is a heat shock protein (HSP) of the GroEL class of HSPs. The gene (htpB) coding the 60-kDa protein was localized to a 3.2-kilobase DNA fragment of L. pneumophila cloned into pUC19 (pSH16) (P. S. Hoffman, C. A. Butler, and F. D. Quinn, Infect. Immu...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Hoffman PS,Houston L,Butler CA

    更新日期:1990-10-01 00:00:00

  • Depletion of Complement Enhances the Clearance of Brucella abortus in Mice.

    abstract::Brucellosis is a bacterial disease of animals and humans. Brucella abortus barely activates the innate immune system at the onset of infection, and this bacterium is resistant to the microbicidal action of complement. Since complement stands as the first line of defense during bacterial invasions, we explored the role...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: González-Espinoza G,Barquero-Calvo E,Lizano-González E,Alfaro-Alarcón A,Arias-Gómez B,Chaves-Olarte E,Lomonte B,Moreno E,Chacón-Díaz C

    更新日期:2018-09-21 00:00:00

  • Matrix metalloproteinase 9 plays a key role in lyme arthritis but not in dissemination of Borrelia burgdorferi.

    abstract::Borrelia burgdorferi, the causative agent of Lyme arthritis, does not produce any exported proteases capable of degrading extracellular matrix despite the fact that it is able to disseminate from a skin insertion site to infect multiple organs. Prior studies have shown that B. burgdorferi induces the host protease, ma...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Heilpern AJ,Wertheim W,He J,Perides G,Bronson RT,Hu LT

    更新日期:2009-07-01 00:00:00

  • Propofol Sedation Exacerbates Kidney Pathology and Dissemination of Bacteria during Staphylococcus aureus Bloodstream Infections.

    abstract::Methicillin-resistant Staphylococcus aureus (MRSA) is responsible for large numbers of postsurgical nosocomial infections across the United States and worldwide. Propofol anesthesia is widely used in surgery and in intensive care units, and recent evidence indicates that even brief exposure to propofol can substantial...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Visvabharathy L,Freitag NE

    更新日期:2017-06-20 00:00:00

  • Candida albicans and Candida stellatoidea, in contrast to other Candida species, bind iC3b and C3d but not C3b.

    abstract::It was demonstrated that complement-coated sheep erythrocytes bind to Candida albicans cells grown in serum-free RPMI 1640 medium. Testing of purified complement components proved that iC3b and C3d were responsible for the reaction, whereas C3b and C3b-H reacted only slightly if at all. Binding occurred only to C. alb...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Heidenreich F,Dierich MP

    更新日期:1985-11-01 00:00:00

  • The Salmonella typhimurium virulence plasmid increases the growth rate of salmonellae in mice.

    abstract::The virulence plasmids of Salmonella typhimurium and other invasive Salmonella serovars have long been associated with the ability of these bacteria to cause systemic infection beyond the intestines in orally inoculated animals. Genetic analysis of virulence genes on the high-molecular-weight plasmids has revealed tha...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Gulig PA,Doyle TJ

    更新日期:1993-02-01 00:00:00

  • Decreased expression of mannose-specific adhesins by Escherichia coli in the colonic microflora of immunoglobulin A-deficient individuals.

    abstract::Most Escherichia coli isolates can express type 1 fimbriae with mannose-specific adhesins. These adhesins bind to the oligosaccharide chains of secretory immunoglobulin A (IgA). Thus, in addition to specific antibody activity, secretory IgA possesses a broad reactivity with bacteria expressing type 1 fimbriae. The abs...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Friman V,Adlerberth I,Connell H,Svanborg C,Hanson LA,Wold AE

    更新日期:1996-07-01 00:00:00

  • Identification of an iron-regulated hemin-binding outer membrane protein, HupO, in Vibrio fluvialis: effects on hemolytic activity and the oxidative stress response.

    abstract::In pathogenic bacteria, iron acquisition is important for colonization and proliferation in the host under iron-limited conditions. The ability of Vibrio spp. to acquire iron is often critical to their virulence, causing gastroenteritis or excessive watery diarrhea in humans. In the study described here, we cloned the...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Ahn SH,Han JH,Lee JH,Park KJ,Kong IS

    更新日期:2005-02-01 00:00:00

  • Comparative translocation of enteropathogenic Campylobacter spp. and Escherichia coli from the intestinal tract of gnotobiotic mice.

    abstract::In C3H gnotobiotic mice, no significant difference was observed between the translocation of nine clinical isolates of enteropathogenic Campylobacter spp. and that of seven nonisogeneic Escherichia coli strains with or without various determinants of pathogenicity. In addition, there was no correlation found between t...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Youssef M,Corthier G,Goossens H,Tancrede C,Henry-Amar M,Andremont A

    更新日期:1987-04-01 00:00:00

  • Requirements for ingestion of Chlamydia psittaci by mouse fibroblasts (L cells).

    abstract::Ingestion of 14C-amino acid-labeled Chlamydia psittaci (6BC) by mouse fibroblasts (L cells) was inhibited when the host cells were incubated for 30 min at 37 degrees C in Earle salts containing 10 mug of crystalline trypsin per ml. Tryptic digestion also inhibited the ingestion of 1-mum polystrene latex beads. Trypsin...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Byrne GI

    更新日期:1976-09-01 00:00:00

  • Chronic Toxoplasma gondii Infection Induces Anti-N-Methyl-d-Aspartate Receptor Autoantibodies and Associated Behavioral Changes and Neuropathology.

    abstract::Anti-NMDA receptor (NMDAR) autoantibodies have been postulated to play a role in the pathogenesis of NMDAR hypofunction, which contributes to the etiology of psychotic symptoms. Toxoplasma gondii is a pathogen implicated in psychiatric disorders and associated with elevation of NMDAR autoantibodies. However, it remain...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Li Y,Viscidi RP,Kannan G,McFarland R,Pletnikov MV,Severance EG,Yolken RH,Xiao J

    更新日期:2018-09-21 00:00:00

  • Essential role of chitinase in the development of the filarial nematode Acanthocheilonema viteae.

    abstract::Chitinases of pathogens have been proposed as potential targets of vaccines or specific inhibitors. We studied the genomic organization, transcript levels, developmental expression, and biological function of chitinases in the rodent filarial nematode Acanthocheilonema viteae, a model organism for human-pathogenic fil...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Tachu B,Pillai S,Lucius R,Pogonka T

    更新日期:2008-01-01 00:00:00

  • A Heterologous Reporter Defines the Role of the Tetanus Toxin Interchain Disulfide in Light-Chain Translocation.

    abstract::Botulinum neurotoxins (BoNTs) and tetanus toxin (TeNT) are the most potent toxins for humans and elicit unique pathologies due to their ability to traffic within motor neurons. BoNTs act locally within motor neurons to elicit flaccid paralysis, while retrograde TeNT traffics to inhibitory neurons within the central ne...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Zuverink M,Chen C,Przedpelski A,Blum FC,Barbieri JT

    更新日期:2015-07-01 00:00:00

  • Activation of neutrophil collagenase in periodontitis.

    abstract::Neutrophil collagenase (matrix metalloproteinase 8 [MMP-8]) is an important mediator of tissue destruction in inflammatory diseases. Studies of anaerobic periodontal infections have shown that active MMP-8 in gingival crevicular fluid is associated with the degradation of periodontal tissues in progressive periodontit...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Romanelli R,Mancini S,Laschinger C,Overall CM,Sodek J,McCulloch CA

    更新日期:1999-05-01 00:00:00

  • Host-etiological agent interactions in intranasally and intraperitoneally induced Cryptococcosis in mice.

    abstract::Inbred CBA/J mice were used in developing a defined in vivo model for studying host-parasite relationships in cryptococcosis. Mice were infected either intranasally or intraperitoneally with 10(3) viable Cryptococcus neoformans cells. At weekly intervals over a 92-day period, C. neoformans growth profiles in the lungs...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lim TS,Murphy JW,Cauley LK

    更新日期:1980-08-01 00:00:00

  • Altered immune function in hypercholesterolemic monkeys.

    abstract::Humoral and cellular immune responses to several antigens were compared in control and hypercholesterolemic groups of monkeys. Chronic hypercholesterolemia, with concomitant hyperphospholipidemia and hypotriglyceridemia, was produced experimentally by feeding monkeys a high-fat, high-cholesterol diet. When studied pri...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Fiser RH Jr,Denniston JC,McGann VG,Kaplan J,Alder WH 3rd,Kastello MD,Beisel WR

    更新日期:1973-07-01 00:00:00