Abstract:
:In previous studies, we reported that fibronectin (FN) markedly enhances phagocytic uptake of antibody-coated group B streptococci (GBS) by human polymorphonuclear leukocytes. Furthermore, administration of FN along with a GBS type-specific monoclonal or polyclonal antibody to infected neonatal rats significantly enhances survival. In this study, we have examined the molecular mechanism of this enhancement through phagocyte receptors which recognize the Arg-Gly-Asp (RGD) peptide sequences contained within the FN molecule. Incubation of human polymorphonuclear leukocytes or culture-derived macrophages on coverslips coated with GRGDSP but not GRGESP markedly enhanced uptake of immunoglobulin G-coated GBS. The enhancing effect of the RGD-containing peptides was blocked by monoclonal antibodies B6H12 (directed against the integrin-associated protein) and 7G2 (directed against the beta 3-integrin receptor for RGD). These data suggest that FN enhancement of antibody-coated GBS uptake is mediated by the critical RGD sequence. Furthermore, this active peptide sequence may have an important role in immunotherapy of bacterial infections, especially in patients with decreased plasma FN concentrations.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Hill HR,Augustine NH,Williams PA,Brown EJ,Bohnsack JFdoi
10.1128/IAI.61.6.2334-2339.1993subject
Has Abstractpub_date
1993-06-01 00:00:00pages
2334-9issue
6eissn
0019-9567issn
1098-5522journal_volume
61pub_type
杂志文章abstract::To analyze the role of interleukin-10 (IL-10) in bacterial cerebral infections, we studied cerebral listeriosis in IL-10-deficient (IL-10(-/-)) and wild-type (WT) mice, the latter of which express high levels of IL-10 in both primary and secondary cerebral listeriosis. IL-10(-/-) mice succumbed to primary as well as s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4561-4571.2001
更新日期:2001-07-01 00:00:00
abstract::Pools of rat antiserum to Plasmodium berghei had different levels of protective activity as assessed by a passive transfer test. Preincubation of parasite inocula with an effective pool before injection did not significantly enhance protective activity. Removal of the antiserum from preincubated parasite inocula aboli...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1213-1218.1974
更新日期:1974-12-01 00:00:00
abstract::Upon infection of a host, the pathogenic fungus Aspergillus fumigatus is attacked by the reactive oxygen species produced by phagocytic cells. Detoxification of hydrogen peroxide by catalases was proposed as a way to overcome this host response. A. fumigatus produces three active catalases; one is produced by conidia,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.6.3551-3562.2003
更新日期:2003-06-01 00:00:00
abstract::The smooth lipopolysaccharide complex of the outer surface of smooth Brucella abortus cells is believed to be the antigenic component involved in serological tests routinely used for the diagnosis of brucellosis. Sera from cattle vaccinated or infected with B. abortus generally contain antibody directed toward the smo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.3.994-1002.1978
更新日期:1978-09-01 00:00:00
abstract::Initiation of a gonococcal infection involves attachment of Neisseria gonorrhoeae to the plasma membrane of an epithelial cell in the mucosal epithelium and its internalization, transepithelial trafficking, and exocytosis from the basal membrane. Piliation and expression of certain Opa proteins and the immunoglobulin ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.2.896-905.2000
更新日期:2000-02-01 00:00:00
abstract::An exoprotein of Staphylococcus aureus 100 that elicited a positive ileal loop response in the rabbit model was investigated in this study. The protein, as it occurred in the culture supernatant fluid, could be detected initially in the late log phase of growth under aerobic and anaerobic conditions. It was stable und...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.2.298-303.1977
更新日期:1977-11-01 00:00:00
abstract::An antigenically distinct serotype, type IV, has recently been added to the recognized serotypes of group B streptococci (GBS). We isolated and purified the capsular polysaccharide antigen from a prototype type IV GBS strain. The type IV capsular polysaccharide formed a precipitin line with rabbit antiserum to type IV...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.4.1089-1094.1989
更新日期:1989-04-01 00:00:00
abstract::Infection of human monocyte-derived macrophages (HMDM) and J774 cells (murine macrophage cell line) with several enteroaggregative and cytodetaching Escherichia coli (EAggEC and CDEC, respectively) strains demonstrated that some strains could induce macrophage cell death accompanied by release of lactate dehydrogenase...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.8.3918-3924.1998
更新日期:1998-08-01 00:00:00
abstract::Chlamydia trachomatis serovar E, the leading bacterial agent responsible for sexually transmitted diseases, is required to invade genital epithelial cells for its growth and survival, yet little is known about the adhesin-receptor interactions promoting its entry. In contrast, much has been published on the heparan su...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.7.3413-3418.2002
更新日期:2002-07-01 00:00:00
abstract::Trypanosoma cruzi is a protozoan parasite that can initiate mucosal infection after conjunctival exposure. The anatomical route of T. cruzi invasion and spread after conjunctival parasite contamination remains poorly characterized. In the present work we have identified the sites of initial invasion and replication af...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00319-06
更新日期:2006-10-01 00:00:00
abstract::Vibrio cholerae O1 has two biotypes, El Tor and Classical, and the latter is now presumed to be extinct in nature. Under carbohydrate-rich growth conditions, El Tor biotype strains produce the neutral fermentation end product 2,3-butanediol (2,3-BD), which prevents accumulation of organic acids from mixed acid ferment...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01237-10
更新日期:2011-08-01 00:00:00
abstract::Immune and pathologic responses were measured for 20 weeks after infection of mice with Brucella abortus 19, RB51, or 2308. Live bacteria and bacterial antigens of 19 and RB51 persisted in spleens for 10 and 4 weeks after infection, respectively, whereas 2308 bacteria and bacterial antigens persisted for at least 20 w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3206-3212.1994
更新日期:1994-08-01 00:00:00
abstract::Immunofluorescence microscopy demonstrated that laminin bound to the surface of Penicillium marneffei conidia. Attachment of P. marneffei conidia in an adherence assay was inhibited by soluble laminin and anti-laminin antibody. N-Acetylneuraminic acid abolished adherence, indicating an interaction mediated by a sialic...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.12.6024-6026.1998
更新日期:1998-12-01 00:00:00
abstract::Cells of several strains of Streptococcus gordonii attached in much higher numbers to experimental pellicles formed from samples of submandibular or parotid saliva on hydroxyapatite (HA) beads than to buffer controls. The nature of the salivary components responsible were investigated by preparing experimental pellicl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.9.2948-2954.1991
更新日期:1991-09-01 00:00:00
abstract::In this study we investigated the mucosal and systemic responses to two T-cell-independent polysaccharides, a serogroup f polysaccharide (formed of rhamnose glucose polymers [RGPs]) from Streptococcus mutans OMZ 175 and a mannan from Saccharomyces cerevisiae, covalently conjugated either to a linear peptide (peptide 3...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2645-2651.1995
更新日期:1995-07-01 00:00:00
abstract::Salmonella infection can cause gastroenteritis in healthy individuals or a serious, systemic infection in immunocompromised patients and has a global impact. CD4 Th1 cells represent the main lymphocyte population that participates in bacterial clearance during both primary and secondary infections in mice of the H-2b ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00588-19
更新日期:2019-10-18 00:00:00
abstract::In order to further understand Epstein-Barr virus (EBV)-lymphocyte interactions, we investigated a chain of events including: (i) EBV binding to human lymphocyte subpopulations; (ii) the earliest appearance of EBV-determined nuclear antigen (EBNA) in the lymphocytes after EBV infection; and (iii) establishment of cont...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.2.303-310.1976
更新日期:1976-02-01 00:00:00
abstract::Vibrio parahaemolyticus is the leading cause of bacterial seafood-borne gastroenteritis worldwide, yet little is known about how this pathogen colonizes the human intestine. The alternative sigma factor RpoN/sigma-54 is a global regulator that controls flagellar synthesis, as well as a wide range of nonflagellar genes...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01210-13
更新日期:2014-02-01 00:00:00
abstract::By subcutaneous treatment with an aqueous solution of 6-O-stearoyl-N-acetylmuramyl-L-alanyl-D-isoglutamine [6-O-CH3-(CH2)16-CO-MurNAc-L-Ala-D-isoGln] [referred to here as L18-MDP(Ala)], an augmentation of the resistance of mice to Escherichia coli, Pseudomonas aeruginosa. Staphylococcus aureus, and Candida albicans in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.292-300.1982
更新日期:1982-07-01 00:00:00
abstract::A parasitic protozoan, Leishmania mexicana amazonensis, was previously made resistant to tunicamycin (J.A. Kink and K.-P. Chang, Proc. Natl. Acad. Sci. USA 84:1253-1257, 1987). In the present study, six different tunicamycin-resistant variants were biologically and biochemically compared with their parental wild type ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.7.1692-1700.1987
更新日期:1987-07-01 00:00:00
abstract::Escherichia coli O157:H7 carries a chromosomal msbB1 and a plasmid-encoded msbB2 gene. We characterized msbB2 function as a homologue of msbB1 by examination of wild-type organisms and mutant strains that lacked functional msbB1, msbB2, and both msbB1 and msbB2. The msbB double-mutant strain generated pentaacyl lipid ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.2.1174-1180.2004
更新日期:2004-02-01 00:00:00
abstract::The gene expression and cytokine release of the proinflammatory cytokines interleukin-1 beta (IL-1 beta), IL-6, and tumor necrosis factor alpha (TNF-alpha) after infection of human epithelial cells (HEp-2 cells) and polymorphonuclear granulocytes (PMNs) were investigated by using isogenic pairs of Listeria monocytogen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.6.2545-2552.1993
更新日期:1993-06-01 00:00:00
abstract::The biological response to endotoxin mediated through the Toll-like receptor 4 (TLR4)-MD-2 receptor complex is directly related to lipid A structure or configuration. Endotoxin structure may also influence activation of the MyD88-dependent and -independent signaling pathways of TLR4. To address this possibility, human...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.5.2940-2950.2005
更新日期:2005-05-01 00:00:00
abstract::Exposure to bacterial superantigens such as staphylococcal enterotoxin B (SEB) leads to the induction of toxic shock syndrome which results in multiorgan failure, including liver damage. In the present study, we investigated the role of CD44 in SEB-induced liver injury. Injection of SEB into d-galactosamine-sensitized...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.50-61.2005
更新日期:2005-01-01 00:00:00
abstract::Exoenzyme Y (ExoY) is a type III secretion system effector found in 90% of the Pseudomonas aeruginosa isolates. Although it is known that ExoY is a soluble nucleotidyl cyclase that increases the cytoplasmic levels of nucleoside 3',5'-cyclic monophosphates (cNMPs) to mediate endothelial Tau phosphorylation and permeabi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00239-17
更新日期:2017-08-18 00:00:00
abstract::Most strains of Shigella flexneri 2a and enteroaggregative Escherichia coli carry a highly conserved chromosomal locus which encodes a 109-kDa secreted mucinase (called Pic) and, on the opposite strand in overlapping fashion, an oligomeric enterotoxin called ShET1, encoded by the setA and setB genes. Here, we characte...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.2915-2925.2002
更新日期:2002-06-01 00:00:00
abstract::Challenges with the production and suboptimal immunogenicity of malaria vaccine candidates have slowed the development of a Plasmodium falciparum multiantigen vaccine. Attempting to resolve these issues, we focused on the use of highly immunogenic merozoite surface protein 8 (MSP8) as a vaccine carrier protein. Previo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00486-17
更新日期:2017-12-19 00:00:00
abstract::A synthetic medium capable of supporting growth of Vibrio parahaemolyticus is described. Growth yields and generation times were comparable to growth in a complex medium, although Kanagawa hemolysin was undetectable in the synthetic medium. Upon the addition of single amino acids to this synthetic medium, only D-trypt...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.60-63.1982
更新日期:1982-07-01 00:00:00
abstract::A series of experiments was conducted to study the influence of embryonal bursectomy (EBX) on the early and late pathogenesis of Marek's disease (MD). The early lytic infection in the lymphoid organs normally associated with oncogenic MD virus infection in intact chickens was not seen in EBX chickens. Therefore, the d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.1.199-207.1981
更新日期:1981-01-01 00:00:00
abstract::A 60-kilodalton (kDa) immunodominant antigen of Legionella pneumophila is a heat shock protein (HSP) of the GroEL class of HSPs. The gene (htpB) coding the 60-kDa protein was localized to a 3.2-kilobase DNA fragment of L. pneumophila cloned into pUC19 (pSH16) (P. S. Hoffman, C. A. Butler, and F. D. Quinn, Infect. Immu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.10.3380-3387.1990
更新日期:1990-10-01 00:00:00