Division of single host cells after infection with chlamydiae.

Abstract:

:Mouse fibroblasts (L cells) were infected in suspension with Chlamydia psittaci (6BC) and then plated out on a solid substrate at a density of 80 cells per cm2 so that the effect of chlamydial infection on the division of single host cells and their progeny could be determined. Uninfected L cells multiplied with a mean generation time of 15 h. The generation time of single L cells infected with 1.5 50% infectious units (ID50) of C. psittaci was over twice as long. Half of the infected L cells had divided once by day 4 after infection, and the rest had divided more than once. Division of infected cells frequently produced one infected and one uninfected daughter. About half of the L cells infected with 15 ID50 of C. psittaci divided at least once before most of them detached from their substrate before observation on day 3. Less than 10% of the L cells infected with 75 ID50 of C. psittaci divided before they were lost from their substrate by day 2. Comparable results were obtained with single L cells infected with a lymphogranuloma venereum (440L) strain of C. trachomatis and with single HeLa 229 cells infected with C. psittaci. It was concluded that high multiplicities of infection of host cells with chlamydiae quickly bring cell division to a halt, whereas lower multiplicities slow but do not immediately stop the division of host cells. However, indefinitely multiplying clones of chlamydia-infected host cells were not observed. The method used here should be applicable to other studies on the division of cells in culture.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Horoschak KD,Moulder JW

doi

10.1128/IAI.19.1.281-286.1978

subject

Has Abstract

pub_date

1978-01-01 00:00:00

pages

281-6

issue

1

eissn

0019-9567

issn

1098-5522

journal_volume

19

pub_type

杂志文章
  • Immunological quantitation of hepatic tryptophan oxygenase in endotoxin-poisoned mice.

    abstract::An apparent inhibition of induction of mouse hepatic tryptophan oxygenase by endotoxin has been reported previously, as evidenced by low catalytic activity. This could be due either to decreased tryptophan oxygenase levels or to inactivation of existing enzyme molecules. To resolve this question, the enzyme was quanti...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.8.4.534-539.1973

    authors: Rippe DF,Berry LJ

    更新日期:1973-10-01 00:00:00

  • Effect of dextranase on the extracellular polysaccharide synthesis of Streptococcus mutans; chemical and scanning electron microscopy studies.

    abstract::A dextranase preparation (AD17) partially purified from a culture liquor of Spicaria violacea strain IFO 6120 significantly inhibited the formation of artifcial dental plaque on a steel wire or on an extracted tooth surface. Changes in the surface morphology of Streptococcus mutans cells due to AD17 action were studie...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.12.6.1415-1425.1975

    authors: Hamada S,Mizuno J,Murayama Y,Ooshima Y,Masuda N

    更新日期:1975-12-01 00:00:00

  • Investigation of the role of type IV Aeromonas pilus (Tap) in the pathogenesis of Aeromonas gastrointestinal infection.

    abstract::Although there is substantial evidence that type IV pili purified from diarrhea-associated Aeromonas species (designated Bfp for bundle-forming pilus) are intestinal colonization factors (S. M. Kirov, L. A. O'Donovan, and K. Sanderson, Infect. Immun. 67:5447-5454, 1999), nothing is known regarding the function of a se...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.7.4040-4048.2000

    authors: Kirov SM,Barnett TC,Pepe CM,Strom MS,Albert MJ

    更新日期:2000-07-01 00:00:00

  • Studies on the effect of starvation on mycobacteria.

    abstract::Ten cultures of Mycobacterium tuberculosis, one of Mycobacterium kansasii (nonsignificant), and one of Mycobacterium phlei were submitted to starvation. As a result they lost first their acid fastness and then all other staining affinities but, in this chromophobic state, they survived for at least 2 years and, after ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.9.5.843-850.1974

    authors: Nyka W

    更新日期:1974-05-01 00:00:00

  • Local passive immunization by monoclonal antibodies against streptococcal antigen I/II in the prevention of dental caries.

    abstract::Local passive immunization with monoclonal antibodies (Mc Ab) to Streptococcus mutans was attempted as an alternative approach to active systemic immunization. We prepared an immunoglobulin G class Mc Ab to the cell surface protein determinant of streptococcal antigen I/II and applied it repeatedly to the teeth of rhe...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.50.3.796-799.1985

    authors: Lehner T,Caldwell J,Smith R

    更新日期:1985-12-01 00:00:00

  • Location of feline leukemia-sarcoma group-specific antigen in infected human tissue culture cells.

    abstract::Feline leukemia-sarcoma group-specific antigens were located in human embryo cells infected with feline leukemia and feline sarcoma viruses. This was done by using the fluorescent-antibody and enzyme-labeled antibody techniques at both light and electron microscopic levels. The antigens were found to be exclusively in...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.5.3.400-405.1972

    authors: Ubertini TR

    更新日期:1972-03-01 00:00:00

  • Fibrinogen cleavage by the Streptococcus pyogenes extracellular cysteine protease and generation of antibodies that inhibit enzyme proteolytic activity.

    abstract::The extracellular cysteine protease from Streptococcus pyogenes is a virulence factor that plays a significant role in host-pathogen interaction. Streptococcal protease is expressed as an inactive 40-kDa precursor that is autocatalytically converted into a 28-kDa mature (active) enzyme. Replacement of the single cyste...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.67.9.4326-4333.1999

    authors: Matsuka YV,Pillai S,Gubba S,Musser JM,Olmsted SB

    更新日期:1999-09-01 00:00:00

  • Contributions of the N- and C-terminal domains of surfactant protein d to the binding, aggregation, and phagocytic uptake of bacteria.

    abstract::Collectins play important roles in host defense against infectious microorganisms. We now demonstrate that the serum collectins mannose-binding lectin (MBL) and conglutinin have less ability to bind to, aggregate, and enhance neutrophil uptake of several strains of gram-negative and gram-positive bacteria than pulmona...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.70.11.6129-6139.2002

    authors: Hartshorn KL,White MR,Crouch EC

    更新日期:2002-11-01 00:00:00

  • Model of experimental chronic osteomyelitis in rats.

    abstract::We describe here a Sprague-Dawley rat model for chronic osteomyelitis. Staphylococcus aureus and sodium morrhuate were implanted by either microdrilling or direct needle injection into the tibiae of rats. Of 107 rats, 87 (81%) developed osteomyelitis when a high-speed drill was used for implantation, and 27 (51%) of 5...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.47.3.581-586.1985

    authors: Rissing JP,Buxton TB,Weinstein RS,Shockley RK

    更新日期:1985-03-01 00:00:00

  • Enterotoxic activity of hemolysin BL from Bacillus cereus.

    abstract::Bacillus cereus causes exotoxin-mediated diarrheal food poisoning. Hemolysin BL (HBL) is a well-characterized B. cereus toxin composed of three components (B, L1, and L2) that together possess hemolytic, cytotoxic, dermonecrotic, and vascular permeability activities. Here, we show that HBL causes fluid accumulation in...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.63.11.4423-4428.1995

    authors: Beecher DJ,Schoeni JL,Wong AC

    更新日期:1995-11-01 00:00:00

  • A C-terminal domain targets the Pseudomonas aeruginosa cytotoxin ExoU to the plasma membrane of host cells.

    abstract::ExoU, a phospholipase injected into host cells by the type III secretion system of Pseudomonas aeruginosa, leads to rapid cytolytic cell death. Although the importance of ExoU in infection is well established, the mechanism by which this toxin kills host cells is less clear. To gain insight into how ExoU causes cell d...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.74.5.2552-2561.2006

    authors: Rabin SD,Veesenmeyer JL,Bieging KT,Hauser AR

    更新日期:2006-05-01 00:00:00

  • FimY does not interfere with FimZ-FimW interaction during type 1 fimbria production by Salmonella enterica serovar Typhimurium.

    abstract::The production of type 1 fimbriae in Salmonella enterica serovar Typhimurium is controlled, in part, by three proteins, FimZ, FimY, and FimW. Amino acid sequence analysis indicates that FimZ belongs to the family of bacterial response regulators of two-component systems. In these studies, we have demonstrated that int...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00795-13

    authors: Zeiner SA,Dwyer BE,Clegg S

    更新日期:2013-12-01 00:00:00

  • Potentiation of human natural killer cell cytotoxicity by Salmonella bacteria is an interferon- and interleukin-2-independent process that utilizes CD2 and CD18 structures in the effector phase.

    abstract::Incubation of large granular lymphocytes (LGL) with glutaraldehyde-fixed bacteria stimulated in the supernatant the production of interferon (IFN), which proved to be mainly IFN-gamma. Even though IFN-gamma was produced upon exposure of LGL to bacteria, anti-IFN-gamma antibodies failed to interfere with induction of c...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.59.8.2767-2773.1991

    authors: Tarkkanen J,Saksela E

    更新日期:1991-08-01 00:00:00

  • Monitoring F1651 P-like fimbria expression at the single-cell level reveals a highly heterogeneous phenotype.

    abstract::F1651 and the pyelonephritis-associated pili (Pap) are two members of the type P family of adhesive factors. They play a key role in establishing disease caused by extraintestinal pathogenic Escherichia coli (ExPEC) strains in animals and humans. Both F1651 and Pap are under the control of an epigenetic and reversible...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.02510-14

    authors: Graveline R,Lavoie R,Garneau P,Daigle F,Sénéchal S,Martin C,Harel J

    更新日期:2015-05-01 00:00:00

  • Comparison of the contributions of heat-labile enterotoxin and heat-stable enterotoxin b to the virulence of enterotoxigenic Escherichia coli in F4ac receptor-positive young pigs.

    abstract::In swine, the most common and severe enterotoxigenic Escherichia coli (ETEC) infections are caused by strains that express K88 (F4)(+) fimbriae, heat-labile enterotoxin (LT), heat-stable enterotoxin b (STb), and enteroaggregative E. coli heat-stable toxin 1. Previous studies based on a design that involved enterotoxin...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01743-07

    authors: Erume J,Berberov EM,Kachman SD,Scott MA,Zhou Y,Francis DH,Moxley RA

    更新日期:2008-07-01 00:00:00

  • In vitro response of human T cells to Pseudomonas aeruginosa.

    abstract::Pseudomonas aeruginosa is a gram-negative bacillus that is a major cause of morbidity and mortality in immunosuppressed patients, burn patients, and patients with cystic fibrosis. Although immunity to these bacteria has been associated with serum antibody, more recent evidence suggests that T-cell-mediated immunity ma...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.40.2.670-674.1983

    authors: Porwoll JM,Gebel HM,Rodey GE,Markham RB

    更新日期:1983-05-01 00:00:00

  • Reactions in the periodontium to continuous antigenic stimulation in sensitized gnotobiotic rats.

    abstract::The purpose of our experiment was to evaluate the destructive potential of a strain of Actinomyces viscosus on the periodontium of sensitized rodents, describing the induced lesions on the basis of quantitative cytology. The experimental design comprised in principle the following procedures. Young germfree rats were ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.10.3.565-577.1974

    authors: Guggenheim B,Schroeder HE

    更新日期:1974-09-01 00:00:00

  • Discordant influence of Blastomyces dermatitidis yeast-phase-specific gene BYS1 on morphogenesis and virulence.

    abstract::Blastomyces dermatitidis is a thermally induced dimorphic fungus capable of causing lung and systemic infections in immunocompetent animal hosts. With the publication of genomic sequences from three different strains of B. dermatitidis and the development of RNA interference as a gene-silencing tool, it has become pos...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01328-09

    authors: Krajaejun T,Wüthrich M,Gauthier GM,Warner TF,Sullivan TD,Klein BS

    更新日期:2010-06-01 00:00:00

  • Histopathological and immunocytochemical studies of Trypanosoma musculi infection of mice.

    abstract::Mice infected with Trypanosoma musculi developed hyperplasia of the spleen, lymph nodes, and liver; in contrast, their thymuses displayed transient involution. All organs returned to normal in a month or less. There was modest anemia, lasting until the parasites were cleared from the bloodstream, followed by a rapid i...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.34.3.1008-1017.1981

    authors: Hirokawa K,Eishi Y,Albright JW,Albright JF

    更新日期:1981-12-01 00:00:00

  • Susceptibility of inbred and outbred mouse strains to Sendai virus and prevalence of infection in laboratory rodents.

    abstract::Sendai virus is one of the more prevalent and serious virus infections of rodents. Infection was found in 66% of the mouse, 63% of the rat, 83% of the hamster, and 44% of the guinea pig colonies examined. Twenty-four inbred and outbred strains of mice were tested for their sensitivity to lethal Sendai virus infection....

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.19.1.123-130.1978

    authors: Parker JC,Whiteman MD,Richter CB

    更新日期:1978-01-01 00:00:00

  • Synthetic peptide immunogens elicit polyclonal and monoclonal antibodies specific for linear epitopes in the D motifs of Staphylococcus aureus fibronectin-binding protein, which are composed of amino acids that are essential for fibronectin binding.

    abstract::A fibronectin (Fn)-binding adhesin of Staphylococcus aureus contains three tandem 37- or 38-amino-acid motifs (D1, D2, and D3), which function to bind Fn. Plasma from patients with S. aureus infections contain antibodies that preferentially recognize ligand induced binding sites in the D motifs and do not inhibit Fn b...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.3.1156-1163.2000

    authors: Huesca M,Sun Q,Peralta R,Shivji GM,Sauder DN,McGavin MJ

    更新日期:2000-03-01 00:00:00

  • Cinematographic observations of growth cycles of Chlamydia trachomatis in primary cultures of human amniotic cells.

    abstract::Time-lapse cinematography was used to study the growth cycle of Chlamydia trachomatis in primary cell cultures of human amnion. Twelve preterm and twelve term placentas were obtained within 8 h of delivery, and epithelial cells were dissociated from the amniotic membranes by trypsinization and grown in Rose chambers. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.7.2042-2047.1990

    authors: Neeper ID,Patton DL,Kuo CC

    更新日期:1990-07-01 00:00:00

  • Species specificity and lack of production of STb enterotoxin by Escherichia coli strains isolated from humans with diarrheal illness.

    abstract::Escherichia coli strains produce at least two heat-stable enterotoxins, STa and STb. STa is well known to be important in the pathogenesis of human diarrheal disease; the role of STb has not been defined. Fifty-two E. coli strains recovered from human diarrheal illness in northeast Brazil or Bangladesh were examined i...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.52.1.323-325.1986

    authors: Weikel CS,Tiemens KM,Moseley SL,Huq IM,Guerrant RL

    更新日期:1986-04-01 00:00:00

  • Effect of antibody on the rickettsia-host cell interaction.

    abstract::A recent study demonstrated that polyclonal antibodies to Rickettsia conorii and monoclonal antibodies to outer membrane proteins A (OmpA) and B (OmpB) provided effective, Fc-dependent, passive immunity, even in severe combined immunodeficient mice with an established infection. In order to determine the mechanism of ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.72.6.3524-3530.2004

    authors: Feng HM,Whitworth T,Popov V,Walker DH

    更新日期:2004-06-01 00:00:00

  • Efficient B cell responses to Borrelia hermsii infection depend on BAFF and BAFFR but not TACI.

    abstract::T cell-independent antibody responses develop rapidly, within 3 to 4 days, and are critical for preventing blood-borne pathogens from evolving into life-threatening infections. The interaction of BAFF, also known as BLyS, with its receptors BAFFR and TACI on B cells is critical for B cell homeostasis and function. Usi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01147-13

    authors: Dickinson GS,Sun G,Bram RJ,Alugupalli KR

    更新日期:2014-01-01 00:00:00

  • Mediation of immunity to Eimeria vermiformis in mice by L3T4+ T cells.

    abstract::Immunity to infection with Eimeria vermiformis was transferred in NIH mice by both the nylon wool-adherent (B-cell-enriched) and nonadherent (T-cell-enriched) fractions of lymphocytes (spleen and mesenteric lymph node) taken from infected donors. Transfer was more variable with the adherent fraction, and when contamin...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.56.7.1760-1765.1988

    authors: Rose ME,Joysey HS,Hesketh P,Grencis RK,Wakelin D

    更新日期:1988-07-01 00:00:00

  • Animal and Human Tissue Models of Vertical Listeria monocytogenes Transmission and Implications for Other Pregnancy-Associated Infections.

    abstract::Intrauterine infections lead to serious complications for mother and fetus, including preterm birth, maternal and fetal death, and neurological sequelae in the surviving offspring. Improving maternal and child heath is a global priority. Yet, the development of strategies to prevent and treat pregnancy-related disease...

    journal_title:Infection and immunity

    pub_type: 杂志文章,评审

    doi:10.1128/IAI.00801-17

    authors: Lowe DE,Robbins JR,Bakardjiev AI

    更新日期:2018-05-22 00:00:00

  • R plasmid in Escherichia coli O103 coding for colonization of the rabbit intestinal tract.

    abstract::One rabbit pathogenic Escherichia coli strain, belonging to serogroup O103, harbors a self-transferable 117-kb plasmid (pREC-1) encoding resistance to several antibiotics. The role of this R plasmid in the colonization of the digestive tract in specific-pathogen-free (E. coli O103-free) rabbits was studied. Five-week-...

    journal_title:Infection and immunity

    pub_type: 杂志文章,收录出版

    doi:10.1128/IAI.59.6.1888-1892.1991

    authors: Reynaud A,Federighi M,Licois D,Guillot JF,Joly B

    更新日期:1991-06-01 00:00:00

  • Comparison of progenitor toxins of nonproteolytic with those of proteolytic Clostridium botulinum Type B.

    abstract::A nonproteolytic strain of Clostridium botulinum type B produces two toxins of different molecular weight (16S and 12S) that are indistinguishable from the corresponding toxins of a proteolytic strain in molecular weight and construction but differ in potential toxicity, activation ratio, and hemagglutinability. Succe...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.13.3.987-989.1976

    authors: Miyazaki S,Kozaki S,Sakaguchi S,Sakaguchi G

    更新日期:1976-03-01 00:00:00

  • Ultrastructure of the invasion of human hair in vitro by the keratinophilic fungus Microsporum gypseum.

    abstract::The pattern of invasion of human hair in vitro by the dermatophyte Microsporum gypseum was studied by transmission and scanning electron microscopy. Mycelia that invaded the hair cortex through the edge of cuticles showed a flattened "frond" growth in contrast to the filamentous form seen on ordinary laboratory media....

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.38.2.706-715.1982

    authors: Kanbe T,Tanaka K

    更新日期:1982-11-01 00:00:00