Abstract:
:Specific proteolytic destruction of the human chemotaxin, C5a, is a property of group A and B streptococcal pathogens. Here we show that virulent group G streptococci from human sources also express C5a peptidase activity. The enzyme responsible for this activity is approximately the same size as and is antigenically similar to that produced by group A streptococci. On the basis of Southern hybridization analysis with an internal fragment of the group A C5a peptidase gene (scpA) as a probe, a copy of this gene was found in the genome of all group G human isolates tested. Comparison of partial restriction maps of scpA and scpG revealed significant similarity between the two genes. Group G strains isolated from dogs and cows were found to lack C5a peptidase activity and did not hybridize to the scpA-specific probe. The association of this activity with three streptococcal species suggests that elimination of phagocyte chemotactic attractants is a more universal virulence mechanism than originally anticipated.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Cleary PP,Peterson J,Chen C,Nelson Cdoi
10.1128/IAI.59.7.2305-2310.1991subject
Has Abstractpub_date
1991-07-01 00:00:00pages
2305-10issue
7eissn
0019-9567issn
1098-5522journal_volume
59pub_type
杂志文章abstract::Amebic erythrophagocytosis is characteristic of invasive amebiasis, and mutants deficient in erythrocyte ingestion are avirulent. We sought to understand the molecular mechanisms underlying erythrocyte phagocytosis by Entamoeba histolytica. Following adherence to amebae, erythrocytes became round and crenulated, and p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3422-3430.2005
更新日期:2005-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00111-09
更新日期:2009-08-01 00:00:00
abstract::Peptic ulcer disease (PUD) occurs after a long-term Helicobacter pylori infection. However, the disease can develop earlier, and rare cases have been observed in children, suggesting that these H. pylori strains may be more virulent. We used suppressive subtractive hybridization for comparative genomics between H. pyl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00123-06
更新日期:2006-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00195-07
更新日期:2007-08-01 00:00:00
abstract::A purified A-antigen preparation of Blastomyces dermatitidis was determined to be composed of five major glycoprotein bands, visible with Coomassie blue and periodic acid-Schiff staining of polyacrylamide gels. At least 20 additional protein bands were detected by using a silver stain, which was 100 times more sensiti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.1.171-177.1981
更新日期:1981-07-01 00:00:00
abstract::The pac gene of the serotype c strain Streptococcus mutans MT8148 encodes a cell surface protein antigen (PAc) of approximate 190 kilodaltons. The serotype c strain S. mutans GS-5 does not produce the 190-kilodalton PAc but produces a lower-molecular-weight protein that reacts with anti-PAc serum. The SphI-BamHI fragm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.2.289-296.1990
更新日期:1990-02-01 00:00:00
abstract::In C3H gnotobiotic mice, no significant difference was observed between the translocation of nine clinical isolates of enteropathogenic Campylobacter spp. and that of seven nonisogeneic Escherichia coli strains with or without various determinants of pathogenicity. In addition, there was no correlation found between t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.4.1019-1021.1987
更新日期:1987-04-01 00:00:00
abstract::The macrophage migration inhibition test was applied to the study of delayed hypersensitivity in mice vaccinated intravenously with oil-treated cell walls of Mycobacterium bovis BCG. Migration inhibition of peritoneal exudate cells from sensitized mice was demonstrated directly upon incubation of the cells with purifi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.1.6.587-594.1970
更新日期:1970-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2416-2419.1987
更新日期:1987-10-01 00:00:00
abstract::Significant resistance against influenza virus and Mycobacterium tuberculosis infections was induced when trehalose dimycolate from M. tuberculosis or M. bovis but not M. avium was combined with muramyl dipeptide. Trehalose dimycolate from M. tuberculosis, in contrast to that from M. avium, could confer resistance aga...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.3.938-940.1985
更新日期:1985-12-01 00:00:00
abstract::A hybridoma produced by the polyethylene glycol fusion of the NS-1 variant of the P3x63Ag8 BALB/c plasmacytoma to splenocytes harvested from a BALB/c mouse immunized with whole gonococci was found to be producing antibody to a common region on gonococcal lipopolysaccharide (LPS). Enzyme-linked immunosorbent assay inhi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.3.751-756.1981
更新日期:1981-12-01 00:00:00
abstract::Encapsulated cells of Cryptococcus neoformans are potent activators of the alternative complement pathway. Previous studies found that monoclonal antibodies (MAbs) specific for the major capsular polysaccharide, termed glucuronoxylomannan (GXM), can markedly suppress the ability of the capsule to accumulate C3 from no...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.4.1547-1553.1998
更新日期:1998-04-01 00:00:00
abstract::At pH 7.4 and in the presence of NaHCO3, human milk and bovine colostrum inhibited the growth of Escherichia coli O111. Adding sufficient iron to saturate the iron-binding capacity of the lactoferrin present in the milk or colostrum prevented bacteriostasis. At pH 6.8 neither molk nor colostrum inhibited E. coli 0111....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.15.2.396-401.1977
更新日期:1977-02-01 00:00:00
abstract::Nitric oxide (NO) produced by inducible NO synthase (iNOS) mediates hypotension in endotoxemia. In this study, NO induction by a toxin-producing Streptococcus pyogenes isolate, H250, and by recombinant streptococcal pyrogenic exotoxin A (rSPEA) has been examined, both in vitro and in vivo. Streptococcal supernatants, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.5.1767-1772.1997
更新日期:1997-05-01 00:00:00
abstract::Inbred mouse strains differ in their susceptibility to infection with the facultative intracellular bacterium Listeria monocytogenes, largely due to delayed or deficient innate immune responses. Previous antibody depletion studies suggested that neutrophils (polymorphonuclear leukocytes [PMN]) were particularly import...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00085-18
更新日期:2018-03-22 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.1.110-116.1979
更新日期:1979-07-01 00:00:00
abstract::We developed a porcine dermal explant model to determine the extent to which Staphylococcus aureus biofilm communities deplete oxygen, change pH, and produce damage in underlying tissue. Microelectrode measurements demonstrated that dissolved oxygen (DO) in biofilm-free dermal tissue was 4.45 ± 1.17 mg/liter, while DO...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.03075-14
更新日期:2015-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.4494-4504.2005
更新日期:2005-08-01 00:00:00
abstract::Virulent strains of Yersinia enterocolitica and their plasmidless, avirulent derivatives were examined for their cell surface properties. Increased surface charge and hydrophobicity of Y. enterocolitica were found to be associated with the possession of a 40- to 48-megadalton plasmid. These surface properties were exp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.2.540-543.1984
更新日期:1984-05-01 00:00:00
abstract::The symptoms of Lyme disease are caused by inflammation induced by species of the Borrelia burgdorferisensu lato complex. The various presentations of Lyme disease in the population suggest that differences exist in the intensity and regulation of the host response to the spirochete. Previous work has described correl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00160-20
更新日期:2020-10-19 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00711-15
更新日期:2015-12-07 00:00:00
abstract::The Chagas' disease parasite Trypanosoma cruzi commonly infects humans through skin abrasions or mucosa from reduviid bug excreta. Yet most studies on animal models start with subcutaneous or intraperitoneal injections, a distant approximation of the skin abrasion route. We show here that atraumatic placement of T. cr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01434-08
更新日期:2009-04-01 00:00:00
abstract::Actinobacillus pleuropneumoniae, the causative agent of swine pleuropneumonia, secretes a hemolytic activity which is thought to be a factor involved in the pathogenesis of the disease. The biosynthesis of hemolysin by serotype 1 strain 4074 was strongly dependent on the activity of free Ca2+ in the growth medium. At ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.10.2570-2575.1988
更新日期:1988-10-01 00:00:00
abstract::We characterized outer membrane proteins (OMPs) from selected Campylobacter jejuni, C. coli, and C. fetus strains by two-dimensional gel electrophoresis (2DGE), using isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and by immunoblotting with immune rabbit serum. The flage...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1987-07-01 00:00:00
abstract::Bactericidal/permeability-increasing protein (BPI), a human neutrophil granule protein, has been shown to bind lipopolysaccharide (LPS) and neutralize LPS-mediated cytokine production in adherent monocytes and the whole-blood system. In this study we investigated the ability of recombinant human BPI (rBPI) to inhibit ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3930-3936.1994
更新日期:1994-09-01 00:00:00
abstract::The complement-fixing (CF) activity of antigens from cultured Burkitt lymphoma cells was determined by using normal American sera as the source of antibody. Approximately 75% of the sera fixed complement with the positive cell lines. These lines contained the herpes-like virus detectable by electron microscopy. The co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.1.20-22.1971
更新日期:1971-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.6891-6895.2000
更新日期:2000-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.2.973-984.2003
更新日期:2003-02-01 00:00:00
abstract::Host resistance to African trypanosomiasis is partially dependent on an early and strong T-independent B-cell response against the variant surface glycoprotein (VSG) coat expressed by trypanosomes. The repetitive array of surface epitopes displayed by a monotypic surface coat, in which identical VSG molecules are clos...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.5.2690-2697.2005
更新日期:2005-05-01 00:00:00
abstract::Corynebacteriophage 782, a phage highly related to the beta family of corynebacteriophages but lacking a tox allele, was isolated from a nontoxinogenic clinical isolate of Corynebacterium diphtheriae. Phage 782 exhibits beta immunity but has a wider host range than beta, forming plaques on strains of C. ulcerans and C...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.1.32-35.1985
更新日期:1985-07-01 00:00:00