Abstract:
:At pH 7.4 and in the presence of NaHCO3, human milk and bovine colostrum inhibited the growth of Escherichia coli O111. Adding sufficient iron to saturate the iron-binding capacity of the lactoferrin present in the milk or colostrum prevented bacteriostasis. At pH 6.8 neither molk nor colostrum inhibited E. coli 0111. Adjusting the pH to 7.4 with NaHCO3 resulted in the development of bacteriostatic activity. Adjusting the pH to 7.4 with NaOH was ineffective. Dialyzed colostrum and milk inhibited bacterial growth at pH 6.8 in the absence of added NaHCO3; addition of citrate or iron abolished bacteriostasis. The chromatographic elution profile of tyrosyl-transfer ribonucleic acid (tRNA) from iron-replete E. coli differs significantly from that of tyrosyl-tRNA from iron-deficient organisms. Examination of the elution profile tyrosyl-tRNA from E. coli 0111 growing in colostrum without added NaHCO3 showed that such bacteria were fully replete in iron. The nature of the elution profile of tyrosyl-tRNA also showed that iron was freely available to the bacteria when citrate was added to dialyzed colostrum but not available in its absence, even at pH 6.8. Results support the idea that the bacteriostatic action of milk and colostrum, due to the combined action of antibody and lactoferrin, depends on the addition of bicarbonate to counteract the iron-mobilizing effect of the citrate normally present in these secretions.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Griffiths E,Humphreys Jdoi
10.1128/IAI.15.2.396-401.1977keywords:
subject
Has Abstractpub_date
1977-02-01 00:00:00pages
396-401issue
2eissn
0019-9567issn
1098-5522journal_volume
15pub_type
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.1.280-289.1994
更新日期:1994-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.3.707-713.1980
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journal_title:Infection and immunity
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doi:10.1128/IAI.24.2.427-433.1979
更新日期:1979-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.8.3238-3243.1992
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journal_title:Infection and immunity
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doi:10.1128/IAI.58.10.3445-3447.1990
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.7.2564-2569.1995
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journal_title:Infection and immunity
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doi:10.1128/IAI.63.4.1246-1252.1995
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.755-761.1983
更新日期:1983-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.3.441-444.1975
更新日期:1975-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.2.518-521.1984
更新日期:1984-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.1.183-188.1977
更新日期:1977-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.97-102.1993
更新日期:1993-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.88-98.1978
更新日期:1978-04-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1991-04-01 00:00:00
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更新日期:1996-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.348-354.1979
更新日期:1979-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2009-02-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2000-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.5.644-654.1970
更新日期:1970-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1996-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05431-11
更新日期:2011-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.2.876-886.2006
更新日期:2006-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.5.1360-1368.1990
更新日期:1990-05-01 00:00:00