Abstract:
:Heat-labile enterotoxins from Escherichia coli strains of porcine and human origin polymerize on heating to form high-molecular-weight aggregates, "procoligenoids," analogous to procholeragenoid derived from the cholera enterotoxin. This aggregation is accompanied by loss of biological activity (toxicity). Further heating results in the release of B-subunit oligomers, coligenoids, analogous to choleragenoid. Further studies are needed to determine whether, like procholeragenoid, the procoligenoids are superior antigens in stimulating gut immunity after parenteral administration.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Finkelstein RA,Sciortino CV,Rieke LC,Burks MF,Boesman-Finkelstein Mdoi
10.1128/IAI.45.2.518-521.1984subject
Has Abstractpub_date
1984-08-01 00:00:00pages
518-21issue
2eissn
0019-9567issn
1098-5522journal_volume
45pub_type
杂志文章abstract::Mycobacterium avium subsp. paratuberculosis is the causative agent of Johne's disease in cattle. The complex, multifaceted interaction of M. avium subsp. paratuberculosis with its host includes dampening the ability of infected cells to respond to stimuli that promote M. avium subsp. paratuberculosis clearance. By dis...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00785-12
更新日期:2013-01-01 00:00:00
abstract::In this study we have investigated the antibody and CD4 T-cell responses to the well-characterized malaria vaccine candidate MSP-1 during the course of a primary Plasmodium chabaudi chabaudi (AS) infection. Specific antibody responses can be detected within the first week of infection, and CD4 T cells can be detected ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2245-2251.2001
更新日期:2001-04-01 00:00:00
abstract::Lymphocyte blastogenesis assays and immunoblotting were used to investigate and compare murine B-cell responses to preparations of extracellular membrane blebs (BAg) and spirochetes (Ag) of Borrelia burgdorferi. Immunoblotting BAg, Ag, and medium control preparations with serum from naive and infected C57BL/10 mice re...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.4.1460-1467.1993
更新日期:1993-04-01 00:00:00
abstract::The role of capsule in the adherence of Haemophilus influenzae type b to human epithelial cells in vitro was examined. A group of 30 nonadherent isolates did not differ in degree of encapsulation compared with their respective adherent variants. Furthermore, capsule-deficient mutants of both nonadherent and adherent v...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.2308-2310.1987
更新日期:1987-09-01 00:00:00
abstract::ExoU is a potent effector protein that causes rapid host cell death upon injection by the type III secretion system of Pseudomonas aeruginosa. The N-terminal half of ExoU contains a patatin-like phospholipase A(2) (PLA(2)) domain that requires the host cell cofactor superoxide dismutase 1 (SOD1) for activation, while ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00223-10
更新日期:2010-08-01 00:00:00
abstract::Group A streptococcal pyrogenic exotoxin (SPE) type C, produced by strain T18P grown in the presence of 32P, was separated from culture supernatant fluids by using alcohol precipitation. The resulting toxin (EtOH-1) contained 3 X 10(6) to 5 X 10(6) cpm of 32P per milligram of protein. The radiolabel migrated with SPE ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.2.585-589.1979
更新日期:1979-11-01 00:00:00
abstract::Detoxified mutants of the Escherichia coli heat-labile toxin (LT) act as mucosal adjuvants to intranasally presented coadministered antigens. Here, we compare the adjuvant activity of a panel of detoxified derivatives of LT, using both intranasal (i.n.) and oral (p.o.) routes of administration. The mutants used as adj...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4400-4406.1999
更新日期:1999-09-01 00:00:00
abstract::Humoral immune responses in experimental African trypanosomiasis were assessed in rabbits infected with Trypanosoma rhodesiense. Immune complexes as measured by a Clq binding assay or a Clq solid-phase assay were detected by day 10 and increased progressively through day 28. Immune complexes analyzed by double diffusi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.2.407-414.1981
更新日期:1981-08-01 00:00:00
abstract::A number of studies have substantiated the pivotal role of innate defense mechanisms in protection against invasive aspergillosis. However, experiments demonstrating increased resistance to lethal intravenous (i.v.) infection with Aspergillus fumigatus conidia in cortisone-treated or untreated mice preinfected with a ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3791-3802.1993
更新日期:1993-09-01 00:00:00
abstract::Parasite antigens which bind to host cell molecules of approximately 32 and 34 kilodaltons (kDa) were identified in supernatant fluids obtained from axenic cultures of Trypanosoma cruzi. These parasite components were first detected in culture supernatants obtained after 2 weeks in culture. Immunoblot analysis of cult...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1812-1816.1990
更新日期:1990-06-01 00:00:00
abstract::Current immunization strategies, using peptide or protein antigens, generally fail to elicit cytotoxic-T-lymphocyte responses, since these antigens are unable to access intracellular compartments where loading of major histocompatibility complex class I (MHC-I) molecules occurs. In an attempt to circumvent this, we in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.6.3249-3258.2002
更新日期:2002-06-01 00:00:00
abstract::Strain superinfection occurs when a second pathogen strain infects a host already infected with a primary strain. The selective pressures that drive strain divergence, which underlies superinfection, and allow penetration of a new strain into a host population are critical knowledge gaps relevant to shifts in infectio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02537-14
更新日期:2014-12-01 00:00:00
abstract::Studies of meningococcal pathogenesis have been severely restricted due to the absence of an adequate animal model. Given the significance of iron in meningococcal pathogenesis, we developed a model of Neisseria meningitidis colonization in outbred adult mice that included daily administration of iron dextran. While r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.1849-1855.2003
更新日期:2003-04-01 00:00:00
abstract::Conventional dendritic cells (cDCs) are critical for protection against pulmonary infection with the opportunistic fungal pathogen Cryptococcus neoformans; however, the role of plasmacytoid dendritic cells (pDCs) is unknown. We show for the first time that murine pDCs have direct activity against C. neoformans via rea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00103-16
更新日期:2016-08-19 00:00:00
abstract::Extracellular protease activity was measured in liquid cultures of representative strains from six serotypes of Legionella pneumophila. A variety of substrates were degraded, including denatured casein, skim milk, gelatin, and hide powder azure, but not elastin. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.1.299-302.1981
更新日期:1981-10-01 00:00:00
abstract::Lower genital tract infection with Chlamydia trachomatis and C. muridarum can induce long-lasting hydrosalpinx in the upper genital tract of women and female mice, respectively. However, A/J mice were highly resistant to induction of long-lasting hydrosalpinx by C. muridarum. We further compared host inflammatory resp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00055-14
更新日期:2014-07-01 00:00:00
abstract::Coagulation and inflammation are interconnected, suggesting that coagulation plays a key role in the inflammatory response to pathogens. A phenome-wide association study (PheWAS) was used to identify clinical phenotypes of patients with a polymorphism in coagulation factor X. Patients with this single nucleotide polym...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00031-19
更新日期:2019-04-23 00:00:00
abstract::The pathogenicities of three proteases from Serratia marcescens, two proteases from Pseudomonas aeruginosa, and one thermolysin from Bacillus stearothermophilus were examined. All proteases tested caused acute liquefactive necrosis of the cornea and descemetocele formation in guinea pig eyes after intrastromal injecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.10.2509-2517.1987
更新日期:1987-10-01 00:00:00
abstract::The effects of phagocytosis-stimulating factor (PSF) derived from polymorphonuclear neutrophils on macrophage functions were studied. PSF enhanced the initial rate of phagocytosis of serum-opsonized zymosan particles by macrophages, whereas it did not affect the phagocytosis of immunoglobulin G-sensitized and inert zy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.8.1762-1766.1987
更新日期:1987-08-01 00:00:00
abstract::Infection by Shigella flexneri is characterized by infiltration of neutrophils in the intestinal mucosa and by a strong inflammatory reaction. Although neutrophils are constitutively programmed to die by apoptosis, we show that isolated human neutrophils undergo necrosis 2 h after infection with virulent S. flexneri s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1289-1296.2000
更新日期:2000-03-01 00:00:00
abstract::The derivative toxins purified from cultures of proteolytic strains of Clostridium botulinum types A and F were found to have been only partially nicked but were fully activated. Trypsinization of C. botulinum type B derivative toxin at pH 6.0 resulted in simultaneous activation and nicking, whereas at pH 4.5, activat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.2.402-407.1977
更新日期:1977-08-01 00:00:00
abstract::Genomic DNA fingerprint analysis was performed on 39 Staphylococcus aureus and 28 Enterococcus faecalis endophthalmitis isolates collected from multiple clinical centers. Among 21 S. aureus genomic DNA fingerprint patterns identified, five clonotypes were recovered from multiple unrelated patients and accounted for 58...
journal_title:Infection and immunity
pub_type: 杂志文章,多中心研究
doi:10.1128/IAI.66.1.356-360.1998
更新日期:1998-01-01 00:00:00
abstract::Phenethyl alcohol, at the maximum concentration which did not inhibit growth (0.3% [vol/vol]), inhibited the production of alpha-lysin and exoproteases but not that of delta-lysin in Staphylococcus aureus Wood 46. The inhibition of alpha-lysin was reversible, and transient accumulation of cell-associated alpha-lysin o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.1.112-117.1985
更新日期:1985-01-01 00:00:00
abstract::Most oral bacteria tested formed colonies on a chemically defined medium with a chromogenic arylsulfatase substrate. Arylsulfatase activity was, however, restricted to Campylobacter-Wolinella group organisms, including Wolinella recta, a possible periodontopathogen. W. recta was the only arylsulfatase-active species a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1380-1383.1989
更新日期:1989-05-01 00:00:00
abstract::To serially examine the immunopathogenesis and histopathology of infection with Chlamydia pneumoniae, we inoculated two cynomolgus monkeys in the conjunctival sac, nose, and nasopharynx with C. pneumoniae TWAR. After inoculation, C. pneumoniae was isolated from the inoculation sites and the rectums of both monkeys for...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.3.593-597.1990
更新日期:1990-03-01 00:00:00
abstract::By using in vitro assays, a group of related, filamentous gram-negative bacteria isolated from subgingival plaque deposits of patients with periodontal disease were found to colonize intact teeth. Tentatively identified as members of the genus Cytophaga, these isolates exhibited a preference for colonizing the cementu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.15-18.1979
更新日期:1979-10-01 00:00:00
abstract::Invasive fungal infections represent an increasing problem associated with high mortality. The present study was undertaken to identify leukocyte subsets that are activated by hyphal fragments in a whole-human-blood model, as well as to examine the involvement of CD14 and Toll-like receptors (TLRs) in activation of mo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2402-2406.2001
更新日期:2001-04-01 00:00:00
abstract::Streptococcus suis serotype 2 is a porcine and human pathogen with adhesive and invasive properties. In other streptococci, large surface-associated proteins (>100 kDa) of the MSCRAMM family (microbial surface components recognizing adhesive matrix molecules) are key players in interactions with host tissue. In this s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00359-06
更新日期:2006-11-01 00:00:00
abstract::Tuberculosis is rising in the developing world due to poor health care, human immunodeficiency virus type 1 infection, and the low protective efficacy of the Mycobacterium bovis BCG vaccine. A new vaccination strategy that could protect adults in the developing world from tuberculosis could have a huge impact on publi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.6.3814-3816.2005
更新日期:2005-06-01 00:00:00
abstract::The urease gene of Staphylococcus saprophyticus CCM883 was cloned and expressed in Staphylococcus carnosus TM300. In vitro translation of the cloned DNA sequences revealed six polypeptides (of 70, 47, 29, 27, 20, and 17 kilodaltons) that were associated with enzyme activity. Introduction of the cloned genes into a ure...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.10.2998-3002.1989
更新日期:1989-10-01 00:00:00