Cloning and expression of Staphylococcus saprophyticus urease gene sequences in Staphylococcus carnosus and contribution of the enzyme to virulence.

Abstract:

:The urease gene of Staphylococcus saprophyticus CCM883 was cloned and expressed in Staphylococcus carnosus TM300. In vitro translation of the cloned DNA sequences revealed six polypeptides (of 70, 47, 29, 27, 20, and 17 kilodaltons) that were associated with enzyme activity. Introduction of the cloned genes into a urease-negative mutant of S. saprophyticus restored the virulence of this strain, confirming our previous suggestion (S. Gatermann, J. John, and R. Marre, Infect. Immun. 57:110-116, 1989) that this enzyme is a major virulence factor of the organism and contributes mainly to cystopathogenicity.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Gatermann S,Marre R

doi

10.1128/IAI.57.10.2998-3002.1989

subject

Has Abstract

pub_date

1989-10-01 00:00:00

pages

2998-3002

issue

10

eissn

0019-9567

issn

1098-5522

journal_volume

57

pub_type

杂志文章
  • Monoclonal antibody to human renal glomeruli cross-reacts with streptococcal M protein.

    abstract::Two murine monoclonal antibodies (immunoglobulin M) evoked against human kidney tissue were examined for cross-reactivity with group A streptococci. A glomerulus-specific antibody, PMII.40.H.2, cross-reacted in an enzyme-linked immunosorbent assay with purified pepsin-extracted M proteins from type 6 and 12 streptococ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.55.10.2416-2419.1987

    authors: Goroncy-Bermes P,Dale JB,Beachey EH,Opferkuch W

    更新日期:1987-10-01 00:00:00

  • Rapid protection of gnotobiotic pigs against experimental salmonellosis following induction of polymorphonuclear leukocytes by avirulent Salmonella enterica.

    abstract::Oral inoculation of 5-day-old gnotobiotic pigs with Salmonella enterica serovar Typhimurium strain F98 resulted in severe enteritis and invasive disease. Preinoculation 24 h earlier with an avirulent mutant of Salmonella enterica serovar Infantis (1326/28) completely prevented disease for up to 14 days (when the exper...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.4.2182-2191.2003

    authors: Foster N,Lovell MA,Marston KL,Hulme SD,Frost AJ,Bland P,Barrow PA

    更新日期:2003-04-01 00:00:00

  • Systematic evaluation of Mycobacterium tuberculosis proteins for antigenic properties identifies Rv1485 and Rv1705c as potential protective subunit vaccine candidates.

    abstract::Lack of efficacious vaccines against Mycobacterium tuberculosis (MTB) infection is a limiting factor in the prevention and control of tuberculosis (TB), the leading cause of death from an infectious agent. Improvement or replacement of the BCG vaccine with one that reliably protects all age-groups is urgent. Concerns ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00585-20

    authors: Wang Y,Li Z,Wu S,Fleming J,Li C,Zhu G,Chen B,Ren B,Wang X,Du B,Li P,Hu P,Yang J,Liu Y,Zhou C,Zhang XE,Bi L,Zhang H,Yang J,Zhang Z

    更新日期:2020-12-14 00:00:00

  • Synthesis of a conjugate vaccine composed of pneumococcus type 14 capsular polysaccharide bound to pertussis toxin.

    abstract::Type 14 is one of the common types isolated from patients of all ages with infections caused by Streptococcus pneumoniae. Its capsular polysaccharide (Pn14) is composed of a neutrally charged tetrasaccharide repeat unit. Pn14 does not elicit protective levels of antibodies in infants and children and is a less than op...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.60.9.3528-3532.1992

    authors: Schneerson R,Levi L,Robbins JB,Bryla DM,Schiffman G,Lagergard T

    更新日期:1992-09-01 00:00:00

  • Ultrastructural-immunohistochemical evidence for a maturation defect of temperature-sensitive G31 vesicular stomatitis virus in murine spinal cord neurons.

    abstract::Ultrastructural immunoperoxidase studies were done in spinal cords of mice infected with wild type vesicular stomatitis virus or its temperature-sensitive (ts) mutant G31. Infected neurons showed subplasmalemmal staining of viral antigen and staining of viral particles budding from the neuronal membrane in wild-type v...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.24.1.276-281.1979

    authors: Dal Canto MC,Rabinowitz SG,Johnson TC,Hughes JV

    更新日期:1979-04-01 00:00:00

  • Massive destruction of malaria-parasitized red blood cells despite spleen closure.

    abstract::It is currently accepted that malaria-parasitized red blood cells (pRBC) are eliminated, like senescent erythrocytes, phagocytically by macrophages in the red pulp of the spleen. Here, however, we show that self-healing Plasmodium chabaudi malaria activates spleen closure in C57BL/6 mice. Confocal laser scanning micro...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.10.6390-6398.2005

    authors: Krücken J,Mehnert LI,Dkhil MA,El-Khadragy M,Benten WP,Mossmann H,Wunderlich F

    更新日期:2005-10-01 00:00:00

  • Bacteriological study of periodontal lesions in two sisters with juvenile periodontitis and their mother.

    abstract::A total of five bacteriological samples from the periodontal pockets of two sisters with localized juvenile periodontitis and their mother with advanced periodontitis was studied. Gram-negative anaerobic rods were predominant in the samples. Bacteroides intermedius and Bacteroides loescheii were the most predominant s...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.45.1.118-121.1984

    authors: Okuda K,Naito Y,Ohta K,Fukumoto Y,Kimura Y,Ishikawa I,Kinoshita S,Takazoe I

    更新日期:1984-07-01 00:00:00

  • Complement receptor 1 expression on mouse erythrocytes mediates clearance of Streptococcus pneumoniae by immune adherence.

    abstract::Complement-containing immune complexes can be presented to phagocytes by human erythrocytes bearing complement receptor 1 (CR1). Although this has long been assumed to be a mechanism by which humans are able to protect themselves from "extracellular" bacteria such as pneumococci, there is little direct evidence. In th...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01263-09

    authors: Li J,Wang JP,Ghiran I,Cerny A,Szalai AJ,Briles DE,Finberg RW

    更新日期:2010-07-01 00:00:00

  • Role of the surface coat in in vitro attachment and phagocytosis of Plasmodium berghei by peritoneal macrophages.

    abstract::Evidence is presented to indicate that Plasmodium berghei merozoites, but not trophozotites, have an antiphagocytic capsule. The capsule appears to form around the developing merozoties of the schizont in the parasitophorous vacuole. Serum from animals immune to P. berghei reacts with this capsule. After reaction with...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.20.3.827-835.1978

    authors: Brooks C,Kreier JP

    更新日期:1978-06-01 00:00:00

  • Saccharomyces boulardii stimulates intestinal immunoglobulin A immune response to Clostridium difficile toxin A in mice.

    abstract::Saccharomyces boulardii is a nonpathogenic yeast that protects against antibiotic-associated diarrhea and recurrent Clostridium difficile colitis. The administration of C. difficile toxoid A by gavage to S. boulardii-fed BALB/c mice caused a 1.8-fold increase in total small intestinal immunoglobulin A levels (P = 0.00...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.4.2762-2765.2001

    authors: Qamar A,Aboudola S,Warny M,Michetti P,Pothoulakis C,LaMont JT,Kelly CP

    更新日期:2001-04-01 00:00:00

  • Complete DNA sequence and analysis of the large virulence plasmid of Shigella flexneri.

    abstract::The complete sequence analysis of the 210-kb Shigella flexneri 5a virulence plasmid was determined. Shigella spp. cause dysentery and diarrhea by invasion and spread through the colonic mucosa. Most of the known Shigella virulence determinants are encoded on a large plasmid that is unique to virulent strains of Shigel...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.5.3271-3285.2001

    authors: Venkatesan MM,Goldberg MB,Rose DJ,Grotbeck EJ,Burland V,Blattner FR

    更新日期:2001-05-01 00:00:00

  • Recruitment of lymphocytes to the lung through vaccination enhances the immunity of mice exposed to irradiated schistosomes.

    abstract::The effector mechanism, which operates against challenge parasites in the lungs of C57BL/6 mice vaccinated once with irradiated cercariae of Schistosoma mansoni, is mediated by CD4+ T helper lymphocytes. However, adoptive transfer of immunity from vaccinated donors to naive recipients by using sensitized T cells has n...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.65.1.42-48.1997

    authors: Coulson PS,Wilson RA

    更新日期:1997-01-01 00:00:00

  • MyD88-dependent responses involving toll-like receptor 2 are important for protection and clearance of Legionella pneumophila in a mouse model of Legionnaires' disease.

    abstract::Legionella pneumophila is a gram-negative facultative intracellular parasite of macrophages. Although L. pneumophila is the causative agent of a severe pneumonia known as Legionnaires' disease, it is likely that most infections caused by this organism are cleared by the host innate immune system. It is predicted that ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.02049-05

    authors: Archer KA,Roy CR

    更新日期:2006-06-01 00:00:00

  • Cloning of Syrian hamster (Mesocricetus auratus) cytokine cDNAs and analysis of cytokine mRNA expression in experimental visceral leishmaniasis.

    abstract::The Syrian golden hamster (Mesocricetus auratus) is uniquely susceptible to a variety of intracellular pathogens and is an excellent model for a number of human infectious diseases. The molecular basis for this high level of susceptibility is unknown, and immunological studies related to this model have been limited b...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.66.5.2135-2142.1998

    authors: Melby PC,Tryon VV,Chandrasekar B,Freeman GL

    更新日期:1998-05-01 00:00:00

  • Protection of mice against the lethal toxicity of a lipopolysaccharide (LPS) by immunization with anti-idiotype antibody to a monoclonal antibody to lipid A from Eikenella corrodens LPS.

    abstract::We produced anti-idiotype antibodies to antibody to lipid A from Eikenella corrodens. The ALA-1 monoclonal antibody (immunoglobulin M [IgM] isotype), which had already been produced in our laboratory (T. Kato, I. Takazoe, and K. Okuda, Infect. Immun. 57:656-659, 1989), had reacted strongly with lipid A from E. corrode...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.58.2.416-420.1990

    authors: Kato T,Takazoe I,Okuda K

    更新日期:1990-02-01 00:00:00

  • Molecular analysis of asymptomatic bacteriuria Escherichia coli strain VR50 reveals adaptation to the urinary tract by gene acquisition.

    abstract::Urinary tract infections (UTIs) are among the most common infectious diseases of humans, with Escherichia coli responsible for >80% of all cases. One extreme of UTI is asymptomatic bacteriuria (ABU), which occurs as an asymptomatic carrier state that resembles commensalism. To understand the evolution and molecular me...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.02810-14

    authors: Beatson SA,Ben Zakour NL,Totsika M,Forde BM,Watts RE,Mabbett AN,Szubert JM,Sarkar S,Phan MD,Peters KM,Petty NK,Alikhan NF,Sullivan MJ,Gawthorne JA,Stanton-Cook M,Nhu NT,Chong TM,Yin WF,Chan KG,Hancock V,Ussery DW

    更新日期:2015-05-01 00:00:00

  • Role of CD44 and its v7 isoform in staphylococcal enterotoxin B-induced toxic shock: CD44 deficiency on hepatic mononuclear cells leads to reduced activation-induced apoptosis that results in increased liver damage.

    abstract::Exposure to bacterial superantigens such as staphylococcal enterotoxin B (SEB) leads to the induction of toxic shock syndrome which results in multiorgan failure, including liver damage. In the present study, we investigated the role of CD44 in SEB-induced liver injury. Injection of SEB into d-galactosamine-sensitized...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.73.1.50-61.2005

    authors: McKallip RJ,Fisher M,Gunthert U,Szakal AK,Nagarkatti PS,Nagarkatti M

    更新日期:2005-01-01 00:00:00

  • Streptococcus salivarius ATCC 25975 possesses at least two genes coding for primer-independent glucosyltransferases.

    abstract::Fractionation of the culture medium showed that Streptococcus salivarius ATCC 25975 secreted a glucosyltransferase (Gtf) that was primer independent. On the basis of this observation, a gene library of S. salivarius chromosomal DNA cloned into lambda L47.1 was screened for a gene(s) coding for such an activity. As a r...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.63.2.609-621.1995

    authors: Simpson CL,Giffard PM,Jacques NA

    更新日期:1995-02-01 00:00:00

  • Antigenic polypeptide complex from the Melvin strain of Neisseria gonorrhoeae: isolation and properties.

    abstract::An antigenic complex has been isolated in a highly purified from from the Melvin strain of Neisseria gonorrhoeae. The complex has a molecular weight of 9.3 x 10(6) and on sodium dodecyl sulfate-polyacrylamide gel electrophoresis was found to consist of several subunits; the most predominant had the following molecular...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.25.2.635-644.1979

    authors: Karkhanis YD,Anderson RL,Zeltner JY,Carlo DJ,Stoudt TH

    更新日期:1979-08-01 00:00:00

  • Acapsular Pasteurella multocida B:2 can stimulate protective immunity against pasteurellosis.

    abstract::We have previously shown that a Pasteurella multocida cexA mutant (PBA875) was impaired in capsule export and highly attenuated in virulence for mice (J. D. Boyce and B. Adler, Infect. Immun. 68:3463-3468, 2000). In this study we show that immunization with high, but not low, doses of PBA875 can confer significant pro...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.69.3.1943-1946.2001

    authors: Boyce JD,Adler B

    更新日期:2001-03-01 00:00:00

  • Role of Staphylococcus aureus coagulase and clumping factor in pathogenesis of experimental endocarditis.

    abstract::The pathogenic role of staphylococcal coagulase and clumping factor was investigated in the rat model of endocarditis. The coagulase-producing and clumping factor-producing parent strain Staphylococcus aureus Newman and a series of mutants defective in either coagulase, clumping factor, or both were tested for their a...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.63.12.4738-4743.1995

    authors: Moreillon P,Entenza JM,Francioli P,McDevitt D,Foster TJ,François P,Vaudaux P

    更新日期:1995-12-01 00:00:00

  • Oncogenicity of avian leukosis viruses of different subgroups and of mutants of sarcoma viruses.

    abstract::Leukosis viruses of seven subgroups were tested for oncogenicity in chickens susceptible to virus infection and to development of lymphoid leukosis (LL) tumors. All subgroup A viruses and the subgroup B virus tested produced a high incidence of LL and other related neoplasms. Viruses of subgroup C and RAV-61 of subgro...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.15.2.423-428.1977

    authors: Purchase HG,Okazaki W,Vogt PK,Hanafusa H,Burmester BR,Crittenden LB

    更新日期:1977-02-01 00:00:00

  • Cell-mediated immunity to Herpes simplex in humans: lymphocyte cytotoxicity measured by 51-Cr release from infected cells.

    abstract::We assessed cell-mediated immunity to herpes simplex virus type 1 antigen in patients suffering from recurrent cold sores and in a series of healthy controls. Paradoxically, all those subject to recurrent herpetic infections had, without exception, evidence of cell-mediated immunity to herpes antigens. This was demons...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.11.2.355-359.1975

    authors: Russell AS,Percy JS,Kovithavongs T

    更新日期:1975-02-01 00:00:00

  • Trojan horse effect: phagocyte-mediated Streptococcus iniae infection of fish.

    abstract::The salmonid macrophage-like cell line RTS-11 and purified trout pronephros phagocytes were used to analyze in vitro entry and survival of two Streptococcus iniae serotypes. Efficient invasion by S. iniae occurred in both cells, but only the type II strain persisted in pronephros phagocytes for at least 48 h. Ex vivo ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.71.5.2318-2325.2003

    authors: Zlotkin A,Chilmonczyk S,Eyngor M,Hurvitz A,Ghittino C,Eldar A

    更新日期:2003-05-01 00:00:00

  • Enzymatic degradation of H2O2 by Leptospira.

    abstract::The enzymes responsible for reducing H2O2 were surveyed in 49 strains of Leptospira by using semiquantitative assays for catalase and peroxidase. The survey revealed a differential distribution of catalase and peroxidase activities between the two leptospiral complexes. The pathogenic Leptospira interrogans strains ga...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.22.3.672-675.1978

    authors: Corin RE,Boggs E,Cox CD

    更新日期:1978-12-01 00:00:00

  • Experimental Escherichia coli ascending pyelonephritis in rats: active peroral immunization with live Escherichia coli.

    abstract::Peroral immunization with a live strain of Escherichia coli O6K13H1 against experimental ascending pyelonephritis caused by the same strain was studied in rats, and the effect of immunization on antibody titers against the O and K antigens and lipid A was determined. Peroral immunization with live bacteria protected s...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.35.2.647-653.1982

    authors: Mattsby-Baltzer I,Hanson LA,Olling S,Kaijser B

    更新日期:1982-02-01 00:00:00

  • Efficient B cell responses to Borrelia hermsii infection depend on BAFF and BAFFR but not TACI.

    abstract::T cell-independent antibody responses develop rapidly, within 3 to 4 days, and are critical for preventing blood-borne pathogens from evolving into life-threatening infections. The interaction of BAFF, also known as BLyS, with its receptors BAFFR and TACI on B cells is critical for B cell homeostasis and function. Usi...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.01147-13

    authors: Dickinson GS,Sun G,Bram RJ,Alugupalli KR

    更新日期:2014-01-01 00:00:00

  • Local immune response to repeated topical antigen application in the simian labial mucosa.

    abstract::Minor salivary glands of the oral mucosa in healthy monkeys (Macaca fascicularis and Macaca mulatta) contain organized structural units suitable for recognizing and processing antigens. A previous study of M. fascicularis monkeys provided experimental evidence of retrograde access of oral antigens deep into the minor ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.41.1.399-409.1983

    authors: Nair PN,Schroeder HE

    更新日期:1983-07-01 00:00:00

  • Salmonella enterica serovars Typhimurium and Dublin can lyse macrophages by a mechanism distinct from apoptosis.

    abstract::Salmonella enterica serovars Typhimurium and Dublin lysed primary bovine alveolar macrophages and immortalized J774.2 macrophage-like cells in the absence of either the morphological changes or DNA fragmentation characteristic of apoptosis. Macrophage lysis was dependent on a subset of caspases and an intact sipB gene...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/iai.68.6.3744-3747.2000

    authors: Watson PR,Gautier AV,Paulin SM,Bland AP,Jones PW,Wallis TS

    更新日期:2000-06-01 00:00:00

  • Histophilus somni Survives in Bovine Macrophages by Interfering with Phagosome-Lysosome Fusion but Requires IbpA for Optimal Serum Resistance.

    abstract::Histophilus somni is capable of intracellular survival within professional phagocytic cells, but the mechanism of survival is not understood. The Fic motif within the direct repeat (DR1)/DR2 domains of the IbpA fibrillary network protein of H. somni is cytotoxic to epithelial and phagocytic cells, which may interfere ...

    journal_title:Infection and immunity

    pub_type: 杂志文章

    doi:10.1128/IAI.00365-18

    authors: Pan Y,Tagawa Y,Champion A,Sandal I,Inzana TJ

    更新日期:2018-11-20 00:00:00