Trojan horse effect: phagocyte-mediated Streptococcus iniae infection of fish.

Abstract:

:The salmonid macrophage-like cell line RTS-11 and purified trout pronephros phagocytes were used to analyze in vitro entry and survival of two Streptococcus iniae serotypes. Efficient invasion by S. iniae occurred in both cells, but only the type II strain persisted in pronephros phagocytes for at least 48 h. Ex vivo models of opsonin-dependent phagocytosis by pronephros phagocytes demonstrated increased phagocytosis efficacy. Analysis of phagocytes collected from diseased fish demonstrated that approximately 70% of the bacteria contained in the blood during the septic phase of the disease were located within phagocytes, suggesting an in vivo intracellular lifestyle. In addition to the augmented levels of bacteremia and enhanced survival within phagocytes, S. iniae type II induces considerable apoptosis of phagocytes. These variabilities in intramacrophage lifestyle might explain differences in the outcomes of infections caused by different serotypes. The generalized septic disease associated with serotype II strains is linked not only to the ability to enter and multiply within macrophages but also to the ability to cause considerable death of macrophages via apoptotic processes, leading to a highly virulent infection. We assume that the phenomenon of survival within phagocytes coupled to their apoptosis plays a crucial role in S. iniae infection. In addition, it may provide the pathogen an efficient mechanism of translocation into the central nervous system.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Zlotkin A,Chilmonczyk S,Eyngor M,Hurvitz A,Ghittino C,Eldar A

doi

10.1128/iai.71.5.2318-2325.2003

keywords:

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

2318-25

issue

5

eissn

0019-9567

issn

1098-5522

journal_volume

71

pub_type

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