Abstract:
:Peritoneal exudate T lymphocytes from Listeria monocytogenes-immune mice in the presence of the homologous antigen (heat-killed L. monocytogenes) and normal macrophages showed L. monocytogenes-specific proliferative responses. Proliferation was inhibited by macrophages from L. monocytogenes- or Corynebacterium parvum-pretreated mice as well as by exogenous prostaglandin E(2). Macrophage-dependent inhibition of T-cell proliferation-at least in part-could be reversed by addition of indomethacin. When selected L. monocytogenes-immune Lyt T-cell subsets were cultured in the presence of inhibitory macrophages, pretreatment with anti-Lyt 1 antiserum plus complement completely abrogated proliferation and pretreatment with anti-Lyt 2 and anti-Lyt 3 antisera plus complement markedly reduced proliferation. However, a mixture (1:1) of the two preselected Lyt T-cell subsets resulted in complete reconstitution of proliferative responses. In contrast, when L. monocytogenes-immune peritoneal exudate T lymphocytes were treated with anti-Lyt antisera plus complement after culture, only treatment with anti-Lyt 1 antiserum plus complement affected proliferation, suggesting regulatory interactions between Lyt 1(+)23(-) and Lyt 1(-)23(+) T cells during in vitro culture which result in proliferation within the Lyt 1(+)23(-) T-cell subset. After rigorous depletion of residual macrophages and in the presence of indomethacin, pretreatment with anti-Lyt 1 antiserum plus complement, but not with anti-Lyt 2 and 3 antisera plus complement, eliminated proliferation. The data presented indicate that interactions between macrophages and Lyt T-cell subsets regulate L. monocytogenes-specific T-cell activation.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Kaufmann SH,Simon MM,Hahn Hdoi
10.1128/IAI.38.3.907-913.1982subject
Has Abstractpub_date
1982-12-01 00:00:00pages
907-13issue
3eissn
0019-9567issn
1098-5522journal_volume
38pub_type
杂志文章abstract::The complement system plays an important role in the host defense against infection, and the formation of the terminal complement complex on the bacterial surface has been shown to be particularly important in killing of gram-negative bacteria. The gram-negative periodontal pathogen Porphyromonas gingivalis is resista...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00304-06
更新日期:2006-09-01 00:00:00
abstract::Salmonella strains that lack or overproduce DNA adenine methylase (Dam) elicit a protective immune response to different Salmonella species. To generate vaccines against other bacterial pathogens, the dam genes of Yersinia pseudotuberculosis and Vibrio cholerae were disrupted but found to be essential for viability. O...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7610-7615.2001
更新日期:2001-12-01 00:00:00
abstract::The hagfish, Eptatretus stoutii, has been shown to synthesize bactericidal antibodies after injections of gram-negative organisms. These bactericidins could be detected as early as 2 days after the primary injection. Secondary responses were accelerated, although titers were not significantly higher than those reached...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.2.160-166.1971
更新日期:1971-08-01 00:00:00
abstract::Modulation of myelopoiesis by three synthetic muramyl peptides was investigated in vivo. Two adjuvant-active compounds (N-acetylmuramyl dipeptide [MDP] and MDP-butyl-ester) elicited significant responses in DBA/2 mice characterized by a rise in the level of monocyte-macrophage colony-stimulating activity in serum, a p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.1081-1085.1983
更新日期:1983-12-01 00:00:00
abstract::Alpha-toxin, the major cytolysin of Staphylococcus aureus, preferentially attacks human platelets and cultured monocytes, thereby promoting coagulation and the release of interleukin-1 and tumor necrosis factor. Titers of naturally occurring antibodies in human blood are not high enough to substantially inhibit these ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.10.3214-3220.1989
更新日期:1989-10-01 00:00:00
abstract::Listeria monocytogenes, isolated from outbreaks in either human or nonhuman primate populations, was administered orally at doses ranging from 10(6) to 10(10) CFU. Four of 10 treated animals delivered stillborn infants. L. monocytogenes was isolated from fetal tissue, and the pathology was consistent with L. monocytog...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1574-1579.2003
更新日期:2003-03-01 00:00:00
abstract::Actinobacillus pleuropneumoniae can use porcine transferrin as the sole source of iron. Two proteins with molecular masses of approximately 60 kDa (TfbA) and 110 kDa have been shown to specifically bind porcine transferrin; from the TfbA protein, three isoforms from A. pleuropneumoniae serotypes 1, 5, and 7 have been ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.10.3846-3850.1995
更新日期:1995-10-01 00:00:00
abstract::Ribosomes of strain NOR-7 of group B Neisseria meningitidis were isolated by a procedure that included treatment of the cells with sodium dodecyl sulfate, disruption in a French pressure cell, and differential centrifugation. These preparations consisted of 66% ribonucleic acid and 24% protein and sedimented as a sing...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.3.355-363.1972
更新日期:1972-09-01 00:00:00
abstract::Type 1 fimbrial phase-locked mutants of uropathogenic Escherichia coli cystitis isolate F11 were used to assess the role of the invertible element during urinary tract infection. Compared to the wild type, the phase-locked off mutant was attenuated, and constitutive production of type 1 fimbriae by the phase-locked on...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.2.1387-1393.2006
更新日期:2006-02-01 00:00:00
abstract::Previous reports have demonstrated that heat-stable (A-type) capsule on piliated enterotoxigenic Escherichia coli enhances colonization of enterotoxigenic E. coli in the small intestine and enhances virulence of enterotoxigenic E. coli. In this report, four encapsulated enterotoxigenic E. coli strains and one encapsul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.3.737-740.1984
更新日期:1984-09-01 00:00:00
abstract::The antigenically conserved proteins of Treponema pallidum subsp. pallidum and four nonpathogenic cultivatable treponemes were investigated by phase partitioning with the nonionic detergent Triton X-114 and immunoblot analysis. None of the T. pallidum integral membrane proteins identified by phase partitioning (deterg...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.7.1825-1828.1988
更新日期:1988-07-01 00:00:00
abstract::The kinetics of attachment and ingestion of Chlamydia trachomatis serotype L1 by monolayers of McCoy cells were studied by using a method that discriminated between attachment and uptake. When about 1% of the McCoy cells was infected, the proteinase K-resistant chlamydial fraction, regarded as ingested chlamydiae, rea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.930-935.1983
更新日期:1983-12-01 00:00:00
abstract::This study was designed to evaluate the immunogenicity and the protective efficacy of a divalent fusion DNA vaccine encoding both the Brucella abortus L7/L12 protein (ribosomal protein) and Omp16 protein (outer membrane lipoprotein), designated pcDNA3.1-L7/L12-Omp16. Intramuscular injection of this divalent DNA vaccin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.5.2734-2741.2006
更新日期:2006-05-01 00:00:00
abstract::Two Escherichia coli strains (B/M 369 and C-35) belonging to enteropathogenic serogroup O86 were isolated from patients with infantile diarrhea and studied with respect to their cellular adherence properties. Both strains exhibited adherence (Ad+) to HEp-2 and HeLa cell monolayers in vitro and expressed mannose-resist...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.4.1106-1113.1990
更新日期:1990-04-01 00:00:00
abstract::The gram-negative gastric pathogen Helicobacter pylori is equipped with an extraordinarily large set of outer membrane proteins (OMPs), whose role in the infection process is not well understood. The Hop (Helicobacter outer membrane porins) and Hor (Hop-related proteins) groups constitute a large paralogous family con...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00364-09
更新日期:2009-09-01 00:00:00
abstract::When phagocytosis of Staphylococcus aureus by human polymorphonuclear leukocytes (PMN) takes place on the surface of cultured human endothelial cells, the endothelial monolayers are damaged by lysosomal enzymes that are released by the PMN. Because PMN can phagocytose opsonized as well as unopsonized staphylococci on ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.6.1455-1460.1987
更新日期:1987-06-01 00:00:00
abstract::Naturally occurring antibodies for Vibrio fetus with whole-cell and O antigen specificities were detected in sera of mature cattle by means of the agglutination and indirect immunofluorescence reactions. O antibodies occurred in each of 21 sera examined, and whole-cell antibodies, in lower concentrations, occurred in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.5.728-733.1972
更新日期:1972-05-01 00:00:00
abstract::In the present study, the roles of interleukin 12 (IL-12) and IL-18 and their possible interaction during superantigen-induced responses were studied by injection of staphylococcal enterotoxin B (SEB) into mice. These data suggest that the role of IL-12 in SEB-induced responses is limited to sustaining gamma interfero...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5949-5952.2001
更新日期:2001-09-01 00:00:00
abstract::A protein antigen called TR-o was isolated from supernatant of a sonically treated Reiter treponeme. The isolation procedure included anion-exchange chromatography on Whatman DE-52, hydrophobic interaction chromatography on decyl agarose, and finally gel filtration on Ac-A-22 Ultrogel. The fractionations were monitore...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.974-978.1982
更新日期:1982-03-01 00:00:00
abstract::A new strategy for rapidly selecting and testing genetic vaccines has been developed, in which a whole genome library is cloned into a bacteriophage lambda ZAP Express vector which contains both prokaryotic (P(lac)) and eukaryotic (P(CMV)) promoters upstream of the insertion site. The phage library is plated on Escher...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.1.167-174.2006
更新日期:2006-01-01 00:00:00
abstract::Migration of the fungal pathogen Candida albicans across the endothelial cell layer is considered a prerequisite for the invasion of multiple organs occurring in systemic candidiasis. We developed an experimental system in which C. albicans migrates from a luminal compartment across a monolayer of bovine aortic endoth...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5085-5091.1996
更新日期:1996-12-01 00:00:00
abstract::Intracellular multiplication of virulent Legionella pneumophila serogroup 1 was inhibited by human monocyte-derived macrophages activated by a glycoprotein extract of Klebsiella pneumoniae, RU 41.740. Macrophage cultures were infected with L. pneumophila in the presence of immune antibodies on day 7 of culture. Extrac...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.9.2234-2239.1987
更新日期:1987-09-01 00:00:00
abstract::Growth of a streptomycin-resistant strain of Escherichia coli (VL-2) in the presence of 30 microgram of streptomycin per ml resulted in the production by these bacteria of structurally altered, nonfunctional type 1 fimbriae. This strain, when grown in this subinhibitory concentration of streptomycin, became incapable ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.792-797.1981
更新日期:1981-02-01 00:00:00
abstract::Ocular toxoplasmosis is a potentially blinding intraocular inflammation. The intent of this study was to investigate the role of Fas-FasL interaction in a murine model of acquired ocular toxoplasmosis induced by intracameral inoculation of Toxoplasma gondii. Intraocular inflammation, Fas and FasL expression on lymphoc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.928-935.1999
更新日期:1999-02-01 00:00:00
abstract::The obligate intracellular parasite Toxoplasma gondii can actively infect any nucleated cell type, including cells from the immune system. The rapid transfer of T. gondii from infected dendritic cells to effector natural killer (NK) cells may contribute to the parasite's sequestration and shielding from immune recogni...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00069-17
更新日期:2017-09-20 00:00:00
abstract::Immunochemically identical components were isolated from water-soluble phases of five Staphylococcus aureus strains by affinity chromatography on fibrinogen-linked Sepharose 4B. The elution was performed with 1 M MgCl2. The component could be isolated from sonicated preparations of whole cells, cell walls, and extrace...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.3.700-708.1985
更新日期:1985-09-01 00:00:00
abstract::The immunoprotective activity of Klebsiella pneumoniae K2 cell surface preparations and purified capsular polysaccharide was tested in mice. The 50% protective dose (PD50), expressed as capsular polysaccharide content, was 2 ng for cell surface preparations and 50 ng for purified capsular polysaccharide. Both preparat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.2.365-370.1986
更新日期:1986-11-01 00:00:00
abstract::Rhodococcus equi is an intracellular pathogen which causes pneumonia in young horses and in immunocompromised humans. R. equi arrests phagosome maturation in macrophages at a prephagolysosome stage and grows inside a privileged compartment. Here, we show that, in murine macrophages activated with gamma interferon and ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00983-10
更新日期:2011-05-01 00:00:00
abstract::We have shown that a member of the 70-kDa heat shock protein (Hsp70) family is a major target of the humoral immune response during Leishmania donovani infection. A recombinant fusion protein was recognized by sera from 92% (35 of 38) of patients with visceral leishmaniasis, including representatives from each of the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2688-2693.1992
更新日期:1992-07-01 00:00:00
abstract::Strain differences have been postulated to explain the observation that group B Streptococcus type III (GBS III) late-onset disease occurs in only a fraction of colonized infants. To determine the distribution of type-specific polysaccharide antigen (Ag) in GBS III, Ag was measured by rocket immunoelectrophoresis in b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.1.124-129.1984
更新日期:1984-04-01 00:00:00