Abstract:
:Gonococci do not readily cause disseminated infection in mice. To simulate some of the conditions leading to disseminated gonococcal infection in women, we suspended gonococci in mucin plus hemoglobin and studied the development of gonococcal bacteremia. The mucin-hemoglobin mixture was used because the menstruum appears to be involved in dissemination of gonococci from the genital tract during menstruation. Mice did not die after massive inocula of 10(9) gonococci given intraperitoneally in broth, but when gonococci were suspended in mucin (15%) alone, the 50% lethal dose was 10(8.4) and in 15% mucin plus 4% hemoglobin (M/H), the 50% lethal dose fell to 10(6.6). Sublethal doses produced local peritonitis and transient bacteremia. With larger inocula the local peritoneal infection progressed to fatal septicemia. Studies of the mechanism by which M/H lowered the 50% lethal dose showed that systemic clearance mechanisms were compromised, but not enough to account for the total decrease in the 50% lethal dose. If gonococci were given intravenously after intraperitoneal inoculation of M/H, sequestration of gonococci in the peritoneal cavity occurred, suggesting an effect on local peritoneal defenses. The effect on neutrophils appeared most significant, since numbers of neutrophils in the peritoneal fluid were decreased in the presence of M/H and neutrophils were destroyed by M/H in vitro. The serum bactericidal system was not affected. We conclude that M/H promotes gonococcal bacteremia by interference with phagocytosis and intracellular killing of gonococci. The model simulates the disseminated gonococcal infection cases in women which follow pelvic inflammatory disease in its progression from local peritonitis to transient or lethal bacteremia and in factors (mucin and hemoglobin) which enhance infection.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Corbeil LB,Wunderlich AC,Corbeil RR,McCutchan JA,Ito JI Jr,Braude AIdoi
10.1128/IAI.26.3.984-990.1979subject
Has Abstractpub_date
1979-12-01 00:00:00pages
984-90issue
3eissn
0019-9567issn
1098-5522journal_volume
26pub_type
杂志文章abstract::Pseudomonas aeruginosa bacteremia in cancer patients develops from initial gastrointestinal (GI) colonization with translocation into the bloodstream in the setting of chemotherapy-induced neutropenia and GI mucosal damage. We established a reproducible mouse model of P. aeruginosa GI colonization and systemic spread ...
journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.57.4.1271-1275.1989
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abstract::Filifactor alocis is a recently recognized periodontal pathogen; however, little is known regarding its interactions with the immune system. As the first-responder phagocytic cells, neutrophils are recruited in large numbers to the periodontal pocket, where they play a crucial role in the innate defense of the periodo...
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journal_title:Infection and immunity
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doi:10.1128/IAI.16.3.995-1004.1977
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journal_title:Infection and immunity
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doi:10.1128/IAI.53.3.700-701.1986
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.31.3.845-849.1981
更新日期:1981-03-01 00:00:00
abstract::Candida-associated denture stomatitis (DS) is a persistent and chronic oral infection of the denture-bearing palatal mucosa. DS stems from the ability of the fungal opportunistic pathogen Candida albicans to adhere to denture material and invade palatal tissue. Although DS is the most prevalent form of oral candidiasi...
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doi:10.1128/IAI.66.12.6024-6026.1998
更新日期:1998-12-01 00:00:00
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更新日期:2019-05-21 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.2.641-642.1981
更新日期:1981-08-01 00:00:00
abstract::Listeria monocytogenes colonized the gastrointestinal tract of adult germfree rats (10(10) to 10(11)/g, dry weight) within 24 h after oral exposure. Between 3 and 14 days after monoassociation, L. monocytogenes caused a self-limiting pseudomembranous colitis, bacteremia, and infection of the spleen and liver. Monoasso...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.1.323-331.1981
更新日期:1981-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.6.1393-1398.1987
更新日期:1987-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.166-171.1983
更新日期:1983-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.2.840-851.1981
更新日期:1981-05-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.55.4.909-915.1987
更新日期:1987-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.2.419-429.1974
更新日期:1974-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.607-610.1985
更新日期:1985-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1992-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.22.2.378-381.1978
更新日期:1978-11-01 00:00:00
abstract::The mechanisms for acquisition of iron by Haemophilus influenzae and their role in pathogenesis are not known. Heme and nonheme sources of iron were evaluated for their effect on growth of type b and nontypable strains of H. influenzae in an iron-restricted, defined medium. All 13 strains acquired iron from heme, hemo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.721-725.1988
更新日期:1988-04-01 00:00:00
abstract::AMA-1 of Plasmodium falciparum is a promising candidate antigen in malaria vaccine development. In this study, we have mapped the immunodominant T-cell determinants in this antigen by using synthetic peptides. From the amphipathic scores, 17 putative T-cell determinants were identified. Nine of the 17 peptides complem...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.3.1054-1059.1996
更新日期:1996-03-01 00:00:00