Abstract:
:Iron starvation conditions limited the growth of Salmonella typhi, as evidenced by an increase in the lag phase of a culture and a decrease in the number of bacteria reached in the stationary phase. The analysis of the outer membrane of bacteria grown under these conditions identified new protein components with apparent molecular weights of 83,000, 78,000, and 69,000. The extent of induction of these proteins was regulated by increased iron deprivation. Immunoblot analysis showed that the serum of patients with typhoid fever exhibited an immunoglobulin G response to these iron-deprivation-induced proteins. The results of bioassays and DNA-DNA hybridization experiments indicated that pathogenic strains of S. typhi produced enterochelin but not aerobactin. Immunodetection with an anti-FepA antiserum confirmed that one of the induced proteins is the S. typhi analog of the Escherichia coli fepA gene product. These studies suggest a role for iron uptake in the pathogenesis of typhoid fever and confirm the immunogenicity of some of the outer membrane proteins of this pathogen.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Fernandez-Beros ME,Gonzalez C,McIntosh MA,Cabello FCdoi
10.1128/IAI.57.4.1271-1275.1989subject
Has Abstractpub_date
1989-04-01 00:00:00pages
1271-5issue
4eissn
0019-9567issn
1098-5522journal_volume
57pub_type
杂志文章abstract::Allergic-type immune responses, particularly immunoglobulin E (IgE), correlate with protective immunity in human schistosomiasis. To better understand the mechanisms of parasite elimination we examined the immune correlates of protection in baboons (Papio cynocephalus anubis), which are natural hosts for Schistosoma m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.2.636-642.1999
更新日期:1999-02-01 00:00:00
abstract::LipL32 is the major outer membrane protein in pathogenic Leptospira. It is highly conserved throughout pathogenic species and is expressed in vivo during human infection. While these data suggest a role in pathogenesis, a function for LipL32 has not been defined. Outer membrane proteins of gram-negative bacteria are t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01643-07
更新日期:2008-05-01 00:00:00
abstract::Vibrio parahaemolyticus is a gram-negative marine bacterium. A limited population of the organisms causes acute gastroenteritis in humans. Almost all of the clinical V. parahaemolyticus isolates exhibit beta-type hemolysis on Wagatsuma agar, known as the Kanagawa phenomenon (KP). KP is induced by the thermostable dire...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01535-07
更新日期:2008-03-01 00:00:00
abstract::Chronic infections play a significant role in the morbidity and mortality of patients with chronic airflow limitation. By stimulating airway inflammation, persistent infection has the potential to cause airway fibrosis. However, in patient this condition is most typically found in lungs damaged by other factors, such ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.2936-2942.1992
更新日期:1992-07-01 00:00:00
abstract::Clostridium difficile is the leading cause of nosocomial bacterial diarrhea. Glutamine and its stable and highly soluble derivative alanyl-glutamine, have been beneficial in models of intestinal injury. In this study, we extend our work on the mechanisms of Clostridium difficile toxin A (TxA)-induced apoptosis in huma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.1.81-87.2006
更新日期:2006-01-01 00:00:00
abstract::The innate immune response to Pneumocystis infection is not well understood. In this study, normal C57BL/6 mouse alveolar macrophages were found to respond to Pneumocystis murina organisms through Toll-like receptor 2 (TLR2), leading to the nuclear translocation of NF-kappaB and the production of proinflammatory cytok...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.3.1857-1864.2006
更新日期:2006-03-01 00:00:00
abstract::The morphology and pathology of cultured mouse glomeruli were examined at the cellular and subcellular levels after infection with a physiological isotonic L-form of Streptococcus pyogenes type 12 or exposure to streptococcal lipoteichoic acid. These changes, as viewed by light microscopy, were identical regardless of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.3.1144-1151.1983
更新日期:1983-12-01 00:00:00
abstract::Purified beta-toxin from Clostridium perfringens type C was found to be a single polypeptide chain protein with a molecular weight of approximately 30,000. The toxin was heat labile, with 75% of its activity being inactivated by incubation at 50 degrees C for 5 min. Biological activity of the purified toxin was comple...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.678-680.1978
更新日期:1978-08-01 00:00:00
abstract::Antigens extracted from cells of Streptococcus pyogenes T6 and Streptococcus mutans strains AHT, BHT, 10449, OMZ175, and K1R adsorbed to the sarcolemmal sheath of cardiac muscle cells in vitro. Similar preparations from S. salivarius, S. sanguis, Staphylococcus aureus, and Lactobacillus casei had weak or negligible ti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.604-613.1980
更新日期:1980-02-01 00:00:00
abstract::Studies of mice suggest that pneumococcal proteins, including PspA, pneumolysin, PsaA, and CbpA, are promising vaccine candidates. To determine whether these proteins are good mucosal immunogens in humans, adenoidal lymphocytes from 20 children who had adenoidectomies were isolated and tested by ELISpot for antigen-sp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.10.5363-5369.2002
更新日期:2002-10-01 00:00:00
abstract::We have developed an animal model for studying mycobacterial pathogenesis using Mycobacterium marinum and the goldfish, Carassius auratus. Goldfish are injected intraperitoneally with doses between 10(2) and 10(9) CFU of M. marinum organisms. Depending on the dose of M. marinum organisms administered, an acute or chro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2938-2942.1998
更新日期:1998-06-01 00:00:00
abstract::Fibronectin binds to Streptococcus pyogenes, and this binding is inhibited by lipoteichoic acid (LTA). Previous studies have shown that LTA can be released from S. pyogenes by treatment with penicillin. Penicillin released LTA from both S. pyogenes and Staphylococcus aureus; however, the binding of fibronectin correla...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.2.529-535.1986
更新日期:1986-02-01 00:00:00
abstract::We have determined the amounts of specific anti-class 1 outer membrane protein antibodies in sera from 25 patients during the course of systemic meningococcal disease, using purified class 1 protein as the sensitizing antigen in an enzyme-linked immunosorbent assay. The class 1 protein was obtained from a variant of s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.4.1437-1443.1994
更新日期:1994-04-01 00:00:00
abstract::Exposure to bacterial superantigens such as staphylococcal enterotoxin B (SEB) leads to the induction of toxic shock syndrome which results in multiorgan failure, including liver damage. In the present study, we investigated the role of CD44 in SEB-induced liver injury. Injection of SEB into d-galactosamine-sensitized...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.50-61.2005
更新日期:2005-01-01 00:00:00
abstract::The role of CD19(+) CD5(+) and CD19(+) CD5(-) B cell subpopulations in the antibody response to pneumococcal capsular polysaccharides (caps-PSs) is controversial. In the present study, we evaluated the role of human CD19(+) CD5(+) and CD19(+) CD5(-) cell populations in the serotype-specific antibody response to caps-P...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00068-15
更新日期:2015-07-01 00:00:00
abstract::The interferon-inducing capabilities of the three molecular segments of bacteriophage phi6 double-stranded ribonucleic acid increased with increasing molecular weight. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.1.284-285.1974
更新日期:1974-07-01 00:00:00
abstract::In mice infected with virulent blood (trypomastigote) forms of Trypanosoma cruzi, complement depletion with cobra venom factor caused a marked exacerbation of the disease evidenced by significantly increased parasitemia levels and early mortality as compared with those of untreated infected animals. The effect was gre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.1.86-91.1975
更新日期:1975-01-01 00:00:00
abstract::Two phospholipase C fractions were detected in culture supernatants from Pseudomonas aeruginosa ATCC 19660, PAO1, and D10C by preparative polyacrylamide gel electrophoresis. Both hemolytic fractions from strain ATCC 19660 were isolated by polyacrylamide gel electrophoresis and were found to cause paralysis, death, der...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.7.1728-1730.1987
更新日期:1987-07-01 00:00:00
abstract::The in vivo role of endogenous interleukin 12 (IL-12) in modulating intrapulmonary growth of Legionella pneumophila was assessed by using a murine model of replicative L. pneumophila lung infection. Intratracheal inoculation of A/J mice with virulent bacteria (10(6) L. pneumophila cells per mouse) resulted in inductio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.1.65-69.1998
更新日期:1998-01-01 00:00:00
abstract::A variant enterotoxin gene, referred to as sezA+, has been identified. Staphylococcus aureus FRI1106, a staphylococcal enterotoxin type D producer (Sed+), contained HindIII fragments of 3.8 and 9.4 kilobase pairs (kbp) that hybridized in Southern blot analysis to a probe containing only staphylococcal enterotoxin type...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1614-1619.1990
更新日期:1990-06-01 00:00:00
abstract::Reduced-minus-oxidized difference spectra of sonically treated virulent Treponema pallidum disclosed cytochromes of the b anc c types as well as large amounts of flavoprotein. Difference spectra of the carbon monoxide-binding pigment identified cytochrome o as the terminal oxidase. Physiological reduction of the cytoc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.3.885-890.1977
更新日期:1977-06-01 00:00:00
abstract::Piliated Burkholderia (formerly Pseudomonas) cepacia from sputa of cys tic fibrosis patients in Toronto, Canada, were shown earlier to bind to purified mucins and to a protein receptor on epithelial cells via a 22-kDa adhesin located on unique cable pili. However, a second receptor, thought to be lipid in nature, was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.4.1420-1425.1996
更新日期:1996-04-01 00:00:00
abstract::Group A streptococcal cell walls inhibited phagocytosis of polystyrene latex particles by rat peritoneal macrophages in vitro. This inhibition was not accompanied by a measurable loss of cell viability. Group D streptococcal cell walls were relatively ineffective in altering phagocytosis. The effectiveness of group A ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.258-261.1978
更新日期:1978-04-01 00:00:00
abstract::Helicobacter pylori vacuolating cytotoxin (VacA) is a secreted protein that induces vacuolation of epithelial cells. To study VacA structure and function, we immunized mice with purified type s1-m1 VacA from H. pylori strain 60190 and generated a panel of 10 immunoglobulin G1kappa anti-VacA monoclonal antibodies. All ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4329-4336.2001
更新日期:2001-07-01 00:00:00
abstract::Lipooligosaccharide (LOS) is a major surface component of Moraxella catarrhalis and a possible virulence factor in the pathogenesis of human infections caused by this organism. The presence of LOS on the bacterium is an obstacle to the development of vaccines derived from whole cells or outer membrane components of th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7569-7577.2005
更新日期:2005-11-01 00:00:00
abstract::Trypanosoma cruzi is a protozoan parasite that causes human Chagas' disease, a leading source of congestive heart failure in Central and South America. CD8+ T cells are critical for control of T. cruzi infection, and CD8+ T cells recognizing the immunodominant trans-sialidase gene-encoded peptide TSKB20 (ANYKFTLV) acc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00275-10
更新日期:2010-07-01 00:00:00
abstract::Antigen-presenting cells (APCs) are key players in the induction and regulation of immune responses. In Plasmodium falciparum malaria, determination of which cells and pathways are activated in the network of APCs remains elusive. We therefore investigated the effects of a controlled human malaria infection in healthy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00473-15
更新日期:2015-09-01 00:00:00
abstract::The adherence mechanism of Aeromonas caviae Sch3N to HEp-2 cells was initially investigated through four mini-Tn5 mutants that showed a 10-fold decrease in adherence. These mutants lost motility, flagella, and their lipopolysaccharide (LPS) O antigen (O-Ag). Three genes, flmB-neuA-flmD, were found to be interrupted by...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.65-74.2001
更新日期:2001-01-01 00:00:00
abstract::Growth of a streptomycin-resistant strain of Escherichia coli (VL-2) in the presence of 30 microgram of streptomycin per ml resulted in the production by these bacteria of structurally altered, nonfunctional type 1 fimbriae. This strain, when grown in this subinhibitory concentration of streptomycin, became incapable ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.792-797.1981
更新日期:1981-02-01 00:00:00
abstract::Streptococcus suis serotype 2 is an encapsulated bacterium and one of the most important bacterial pathogens in the porcine industry. Despite decades of research for an efficient vaccine, none is currently available. Based on the success achieved with other encapsulated pathogens, a glycoconjugate vaccine strategy was...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00139-16
更新日期:2016-06-23 00:00:00