Abstract:
:Urinary tract infections (UTIs) are among the most common infectious diseases of humans, with Escherichia coli responsible for >80% of all cases. One extreme of UTI is asymptomatic bacteriuria (ABU), which occurs as an asymptomatic carrier state that resembles commensalism. To understand the evolution and molecular mechanisms that underpin ABU, the genome of the ABU E. coli strain VR50 was sequenced. Analysis of the complete genome indicated that it most resembles E. coli K-12, with the addition of a 94-kb genomic island (GI-VR50-pheV), eight prophages, and multiple plasmids. GI-VR50-pheV has a mosaic structure and contains genes encoding a number of UTI-associated virulence factors, namely, Afa (afimbrial adhesin), two autotransporter proteins (Ag43 and Sat), and aerobactin. We demonstrated that the presence of this island in VR50 confers its ability to colonize the murine bladder, as a VR50 mutant with GI-VR50-pheV deleted was attenuated in a mouse model of UTI in vivo. We established that Afa is the island-encoded factor responsible for this phenotype using two independent deletion (Afa operon and AfaE adhesin) mutants. E. coli VR50afa and VR50afaE displayed significantly decreased ability to adhere to human bladder epithelial cells. In the mouse model of UTI, VR50afa and VR50afaE displayed reduced bladder colonization compared to wild-type VR50, similar to the colonization level of the GI-VR50-pheV mutant. Our study suggests that E. coli VR50 is a commensal-like strain that has acquired fitness factors that facilitate colonization of the human bladder.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Beatson SA,Ben Zakour NL,Totsika M,Forde BM,Watts RE,Mabbett AN,Szubert JM,Sarkar S,Phan MD,Peters KM,Petty NK,Alikhan NF,Sullivan MJ,Gawthorne JA,Stanton-Cook M,Nhu NT,Chong TM,Yin WF,Chan KG,Hancock V,Ussery DWdoi
10.1128/IAI.02810-14subject
Has Abstractpub_date
2015-05-01 00:00:00pages
1749-64issue
5eissn
0019-9567issn
1098-5522pii
IAI.02810-14journal_volume
83pub_type
杂志文章abstract::The antihistoplasma activity of recombinant murine gamma interferon (rMuIFN-gamma)-treated macrophages of the RAW 264.7 cell line depends on the generation of nitric oxide (NO.) from L-arginine. Macrophages of the P388D1 cell line treated with rMuIFN-gamma do not produce NO. or inhibit the intracellular growth of Hist...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.4.1478-1479.1994
更新日期:1994-04-01 00:00:00
abstract::We have examined the ability of peptidoglycan (PepG) and lipoteichoic acid (LTA) isolated from Staphylococcus aureus to induce the release of tumor necrosis factor alpha (TNF-alpha), interleukin-6 (IL-6), and IL-10 in whole human blood and identified the cellular origins of these cytokines. Both PepG and LTA induced t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.7.3965-3970.2000
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abstract::In a cross-sectional survey of 187 Gambian children and adults, we have analyzed prevalence, fine specificity, and 19-kilodalton merozoite surface protein 1 (MSP-1(19))-specific erythrocyte invasion inhibitory activity of antibodies to MSP-1(19) but find no significant association between any of these parameters and p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.10.6185-6189.2004
更新日期:2004-10-01 00:00:00
abstract::CmeABC functions as a multidrug efflux pump contributing to the resistance of Campylobacter to a broad range of antimicrobials. In this study, we examined the role of CmeABC in bile resistance and its contribution to the adaptation of Campylobacter jejuni in the intestinal tract of the chicken, a natural host and a ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.8.4250-4259.2003
更新日期:2003-08-01 00:00:00
abstract::The ability of various strains of rats to produce interferon in response to polyriboinosinic-polyribocytodilic acid was investigated. ACI and DA (RT-1a), BN (RT-1n), Buffalo (RT-1b), August (RT-1c), and GH (RT-1l) strains produced low levels of interferon in response to intraperitoneal administration of polyriboinosin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.43.2.580-583.1984
更新日期:1984-02-01 00:00:00
abstract::A rapid and reproducible method of bacteriocin typing is described based on leakage of ultraviolet light-absorbing material (UVAM), detectable in supernatants of bacteriocin-sensitive cultures, by means of a spectrophotometer. The prerequisites for reproducible results, with nonsignificant fluctuations in standard err...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.12-19.1977
更新日期:1977-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00377-16
更新日期:2016-08-19 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.148-153.2001
更新日期:2001-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.41.2.563-569.1983
更新日期:1983-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2449-2451.2004
更新日期:2004-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.1.94-98.1995
更新日期:1995-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.5.2618-2627.2004
更新日期:2004-05-01 00:00:00
abstract::A recent study demonstrated that polyclonal antibodies to Rickettsia conorii and monoclonal antibodies to outer membrane proteins A (OmpA) and B (OmpB) provided effective, Fc-dependent, passive immunity, even in severe combined immunodeficient mice with an established infection. In order to determine the mechanism of ...
journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2004-06-01 00:00:00
abstract::Entamoeba histolytica virulence is related to a number of amebic components (lectins, cysteine proteinases, and amebapore) and host factors, such as intestinal bacterial flora. Trophozoites are selective in their interactions with bacteria, and the parasite recognition of glycoconjugates plays an important role in ame...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.5.2096-2102.1999
更新日期:1999-05-01 00:00:00
abstract::A major ceramide monohexoside (CMH) was purified from lipidic extracts of Cryptococcus neoformans. This molecule was analyzed by high-performance thin-layer chromatography (HPTLC), gas chromatography coupled with mass spectrometry, and fast atom bombardment-mass spectrometry. The cryptococcal CMH is a beta-glucosylcer...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.12.7049-7060.2000
更新日期:2000-12-01 00:00:00
abstract::Staphylococcus aureus Newman with an insertion mutation in clfB, the gene encoding clumping factor B, only marginally decreased infection rate (P>0.05) in rats with experimental endocarditis. In contrast, clfB complementation on a multicopy plasmid significantly increased infectivity (P<0.05) over the deleted mutants....
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.9.5443-5446.2000
更新日期:2000-09-01 00:00:00
abstract::ATP and UTP have been proposed for use as therapeutic treatment of the abnormal ion transport in the airway epithelium in cystic fibrosis (CF), the most characteristic feature of which is permanent infection by Pseudomonas aeruginosa. As for diverse gram-negative bacteria, this pathogenic bacterium accumulates diffusi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5076-5082.1999
更新日期:1999-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.6.1642-1646.1976
更新日期:1976-06-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1994-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.2.283-290.1977
更新日期:1977-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.4151-4158.2004
更新日期:2004-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3638-3643.1997
更新日期:1997-09-01 00:00:00
abstract::Female LSH hamsters infected with Treponema pallidum subsp, endemicum before pregnancy or during early pregnancy transmit a form of syphilis to the fetus that is similar to human congenital syphilis. The offspring develops rhinitis, skin rash, failure to thrive, and hepatosplenomegaly. T. pallidum is detectable in the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.8.3559-3561.1993
更新日期:1993-08-01 00:00:00
abstract::By using a suckling mouse assay, heat-stable enterotoxin (ST) was purified from the culture filtrate of Yersinia enterocolitica isolated from a diarrheal patient. The purification procedures involve ultrafiltration with an Amicon HIP-10 hollow fiber, ethanol fractionation, protamine sulfate treatment, diethylaminoethy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.2.554-559.1981
更新日期:1981-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01612-14
更新日期:2014-10-01 00:00:00
abstract::Streptococcus pneumoniae infections of the lung are associated with significant damage to the alveolar epithelium. Host phagocytes and pneumolysin, a cytolytic toxin of S. pneumoniae, are believed to contribute to this cellular damage, yet experiments in which these elements are absent demonstrate the presence of an a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.10.4392-4397.1993
更新日期:1993-10-01 00:00:00
abstract::Mice with a null deletion mutation in the gamma interferon (IFN-gamma) receptor gene were used to study the role of IFN-gamma responsiveness during experimental pulmonary cryptococcosis. Cryptococcus neoformans was inoculated intratracheally into mice lacking the IFN-gamma receptor gene (IFN-gammaR-/-) and into contro...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.3.1788-1796.2005
更新日期:2005-03-01 00:00:00
abstract::A 60-kilodalton (kDa) immunodominant antigen of Legionella pneumophila is a heat shock protein (HSP) of the GroEL class of HSPs. The gene (htpB) coding the 60-kDa protein was localized to a 3.2-kilobase DNA fragment of L. pneumophila cloned into pUC19 (pSH16) (P. S. Hoffman, C. A. Butler, and F. D. Quinn, Infect. Immu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.10.3380-3387.1990
更新日期:1990-10-01 00:00:00
abstract::Previous studies in our laboratory have indicated that naturally resistant, inbred DBA/2J mice mount a greater serum antibody response to Pseudomonas aeruginosa 19660 than susceptible C57BL/6J mice. However, the specificity of the antibody produced was not known. The present study examines the specificity and kinetics...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.1984-1990.1991
更新日期:1991-06-01 00:00:00