Abstract:
:Group A streptococcal pyrogenic exotoxin (SPE) type C, produced by strain T18P grown in the presence of 32P, was separated from culture supernatant fluids by using alcohol precipitation. The resulting toxin (EtOH-1) contained 3 X 10(6) to 5 X 10(6) cpm of 32P per milligram of protein. The radiolabel migrated with SPE C during isoelectric focusing in polyacrylamide gels (pI 6.7) and double immunodiffusion, in which the toxin formed a line of identity with highly purified SPE C when reacted with hyperimmune antisera raised against SPE C. The EtOH-1 radiolabeled toxin was pyrogenic and had the capacity to enhance host susceptibility to lethal endotoxin shock. EtOH-1 toxin lost both radiolabel and biological activity after being treated with alkaline phosphatase. The nonspecific lymphocyte mitogenicity of purified unlabeled SPE C was stimulated by adenosine monophosphate but not adenosine, adenosine diphosphate, or adenosine triphosphate. Adenosine monophosphate may function as a cofactor of SPE C and contribute the phosphate group required for biological activity.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Schlievert PM,Bettin KM,Watson DWdoi
10.1128/IAI.26.2.585-589.1979keywords:
subject
Has Abstractpub_date
1979-11-01 00:00:00pages
585-9issue
2eissn
0019-9567issn
1098-5522journal_volume
26pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.669-671.1978
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journal_title:Infection and immunity
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更新日期:1991-10-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.2.381-388.1995
更新日期:1995-02-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1978-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1975-06-01 00:00:00
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更新日期:1975-04-01 00:00:00
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更新日期:2005-09-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1993-05-01 00:00:00
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