Abstract:
:Fourteen freshly isolated strains of Streptococcus sanguis were obtained from the dental plaque of five healthy adults. Whole saliva was collected concomitant with the plaque isolates from the five subjects, and a second whole saliva sample was collected 10 weeks later. All possible combinations of the first five saliva samples, the second five saliva samples, and 14 strains of bacteria were tested for aggregation. Of the 140 combinations examined, 108 of 140 (77%) of the strains aggregated with the first saliva samples and 95 of 140 (68%) aggregated with the second saliva samples. Overall, 72% of the strains aggregated with both the first and second saliva samples. Removal of immunoglobulin A (IgA) from these same salivas resulted in 38 of 108 (35%) of the aggregates decreasing in intensity with the first saliva samples and 27 of 95 (29%) of the aggregates decreasing in intensity with the second saliva samples. No aggregates increased in intensity with saliva samples when IgA had been removed. Removal of IgA from saliva also resulted in a mean decrease of 46% in adherence of S. sanguis to hydroxyapatite coated with the IgA-deficient saliva. Several strains of S. sanguis were shown to aggregate strongly with human salivary and colostral IgA. In addition, S. sanguis strain S7 showed a 31% stimulation of adherence to hydroxyapatite precoated with human salivary IgA over the uncoated controls. Stepwise removal of IgA from saliva resulted in a decrease in aggregation intensity from strong (4+) to weak (1+ to 2+). Similarly, the adherence of S. sanguis to hydroxyapatite coated with these saliva samples decreased linearly as the salivary IgA was depleted. Alternatively, the addition of a small quantity of salivary IgA (20 mug/ml) to progressively diluted saliva maintained a high level of adherence and strong aggregation until the saliva dilutions reached between 1:8 in the adherence experiments and 1:32 for the aggregations. These data indicate that salivary IgA may play an important role in the microbial ecology of human dental plaque formation.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Liljemark WF,Bloomquist CG,Ofstehage JCdoi
10.1128/IAI.26.3.1104-1110.1979subject
Has Abstractpub_date
1979-12-01 00:00:00pages
1104-10issue
3eissn
0019-9567issn
1098-5522journal_volume
26pub_type
杂志文章abstract::Immunoglobulin class-specific antibodies were measured by a solid-phase radioimmunoassay in serum, bronchoalveolar washings (BAW), lung cell lysates, and spleen cell lysates in germfree mice after intranasal (i.n.) and intraperitoneal (i.p.) primary and secondary 10(5), 10(4), and 10(3) mean tissue culture infective d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.17.3.521-527.1977
更新日期:1977-09-01 00:00:00
abstract::Toxic shock syndrome (TSS) toxin 1 (TSST1) is produced by strains of Staphylococcus aureus associated with TSS. Purified TSST1 induces in rabbits a shock-like illness with many features similar to TSS in humans. These symptoms were also induced by TSST1-producing bacteria in diffusion chambers implanted in the rabbit ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.50.1.304-309.1985
更新日期:1985-10-01 00:00:00
abstract::Tritrichomonas foetus is a serious veterinary pathogen, causing bovine trichomoniasis, a sexually transmitted disease leading to infertility and abortion. T. foetus infects the mucosal surfaces of the reproductive tract. Infection with T. foetus leads to apoptotic cell death of bovine vaginal epithelial cells (BVECs) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.4151-4158.2004
更新日期:2004-07-01 00:00:00
abstract::Because of the unavailability of a suitable antigen, specific in vitro lymphocyte reactivity has not been investigated in persons who have had systemic blastomycosis. Twelve persons who had recovered from blastomycosis were evaluated, and all were found to exhibit strongly positive and apparently specific in vitro cel...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.2.485-490.1981
更新日期:1981-08-01 00:00:00
abstract::Cells of Candida albicans strain WO-1 and related strains switch frequently and reversibly between a white-colony-forming unit (white phase) and a gray-colony-forming unit (opaque phase). Cells in the budding white phase exhibit the usual smooth round phenotype observed in other C. albicans strains, but cells in the b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.2.458-467.1989
更新日期:1989-02-01 00:00:00
abstract::Curli are thin, coiled fibers expressed on the surface of Escherichia coli that bind several matrix and plasma proteins such as fibronectin, laminin, plasminogen, tissue plasminogen activator, and H-kininogen. In this work, we examined the interactions between curli-expressing E. coli and human major histocompatibilit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.3.944-949.1998
更新日期:1998-03-01 00:00:00
abstract::Several monoclonal antibodies directed against gonococcal pili have been used to investigate the potential contribution of anti-pilus antibodies to host defense against gonococcal infection. Included were two antibodies (SM1 and SM2) which reacted with conserved determinants present on pili from all strains tested and...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.3.621-628.1985
更新日期:1985-09-01 00:00:00
abstract::Actinomyces viscosus ATCC 15987 was examined for the presence of cell-associated levan by absorption of myeloma proteins with antilevan activity and direct immunofluorescence. Levan was not detectable on the surface of glucose-grown A. viscosus, but after a brief incubation of these cells with 5% sucrose, they were en...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.2.711-719.1978
更新日期:1978-02-01 00:00:00
abstract::Spiroplasmas were propagated in the Drosophila melanogaster cell line Dm-1. Spiroplasma citri and unidentified strains (corn shunt organism, 277F [tick isolate], powder puff, BNR-1, honey bee, and OBMG) grew to 10(8) to 10(9) colony-forming units per ml and could be passaged. Cytopathic effect (CPE) varied with the in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.1.296-304.1982
更新日期:1982-01-01 00:00:00
abstract::Toll-like receptor 4 (TLR4), which recognizes lipopolysaccharide from Gram-negative bacteria, plays a major role in resistance of mice and humans to Salmonella infection. In chickens, Salmonella may establish a carrier state whereby bacteria are able to persist in the host organism without triggering clinical signs. B...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00025-11
更新日期:2011-08-01 00:00:00
abstract::Endotoxin may cause abnormal deposition of platelet-endotoxin aggregates, and this event could have damaging effects. We compared the aggregation characteristics of platelets from rabbits made tolerant to the lethal effects of endotoxin with those of platelets from normal rabbits. Platelets from tolerant rabbits aggre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.919-922.1978
更新日期:1978-03-01 00:00:00
abstract::Localized juvenile periodontitis (LJP) runs in families, and a predisposition to develop disease appears to be inherited in an autosomal dominant fashion. Patients with LJP have elevated levels of serum immunoglobulin G2 (IgG2), and this is most striking in black LJP patients. We hypothesized that the markedly elevate...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2004-2009.1996
更新日期:1996-06-01 00:00:00
abstract::We have previously shown that Bacillus anthracis lethal toxin represses glucocorticoid receptor (GR) transactivation. We now report that repression of GR activity also occurs with the large clostridial toxins produced by Clostridium sordellii and C. difficile. This was demonstrated using a transient transfection assay...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00291-07
更新日期:2007-08-01 00:00:00
abstract::Previous examination of thin sections of L929 cells heavily infected with the Q fever Priscilla isolate by conventional transmission electron microscopy indicated that the rickettsiae resided within multiple vacuoles. The present study using high-voltage electron microscopy and three-dimensional reconstruction reveale...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.10.4485-4488.1993
更新日期:1993-10-01 00:00:00
abstract::Strains of indigenous Escherichia coli, Bacteroides, and Lactobacillus were isolated from the gastrointestinal tracts of specific pathogen-free (SPF) mice. Nonvaccinated SPF mice exhibited in their spleens low numbers of plaque-forming cells (PFC) and rosette-forming cells reacting with antigens of these andigenous ba...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.2.320-329.1975
更新日期:1975-02-01 00:00:00
abstract::Treponema hyodysenteriae was shown to attach to mouse peritoneal cells in the absence of serum opsonins in vitro. If serotype-specific antiserum from pigs was added to the media and treponemes of that corresponding serotype were employed in the assay, the amount of attachment increased an average of 3.7 times that of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.38.3.1029-1032.1982
更新日期:1982-12-01 00:00:00
abstract::Infection with Histoplasma capsulatum results in a subclinical infection in immunocompetent hosts due to an effective cellular immune response. By contrast, immunodeficient individuals can have a severe disseminated and potentially fatal disease. In a previous study, it was demonstrated that normal mice infected intra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.3.936-942.1997
更新日期:1997-03-01 00:00:00
abstract::Gram-negative bacterial cells exposed to a complement source may carry membrane attack complexes containing variable numbers of C9 molecules per C5b-8 site. In order to investigate the assembly of this complex, the ability of C9 molecules to bind to C5b-8 complexes was compared with the binding characteristics of C9 f...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.7.2800-2805.1994
更新日期:1994-07-01 00:00:00
abstract::Conditions for the release of streptococcal type 12 M protein from whole cells by cyanogen bromide are described; they demonstrated that methionine is not essential to the structural arrangements which account for some of its immunological and biological properties. The released M protein was separated from other prot...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.2.546-555.1978
更新日期:1978-08-01 00:00:00
abstract::A 46-kDa hemolytic protein, referred to as cystalysin, from Treponema denticola ATCC 35404 was overexpressed in Escherichia coli LC-67. Both the native and recombinant 46-kDa proteins were purified to homogeneity. Both proteins expressed identical biological and functional characteristics. In addition to its biologica...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.8.3231-3238.1997
更新日期:1997-08-01 00:00:00
abstract::Inbred mouse strains differ in their susceptibility to infection with the facultative intracellular bacterium Listeria monocytogenes, largely due to delayed or deficient innate immune responses. Previous antibody depletion studies suggested that neutrophils (polymorphonuclear leukocytes [PMN]) were particularly import...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00085-18
更新日期:2018-03-22 00:00:00
abstract::Eight chlamydial isolates of ovine origin were tested in a plaque reduction system using homologous and heterologous rooster antisera. The eight isolates could be separated into two separate immunotypes. Type 1 included isolates associated with ovine abortion and one agent recovered from the feces of an apparently nor...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.1.92-94.1974
更新日期:1974-01-01 00:00:00
abstract::Infection with Cryptosporidium parvum, a ubiquitous protozoan parasite of virtually all mammals, can cause mild to severe diarrhea in immunocompetent hosts and life-threatening diarrhea in immunocompromised hosts. Passive immunotherapy of experimentally infected animals and naturally infected humans with hyperimmune b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.2962-2965.1990
更新日期:1990-09-01 00:00:00
abstract::Phagocytosis and killing by alveolar macrophages from humans, rabbits, rats, and hamsters, were compared in vitro. In the absence of serum opsonins, human alveolar macrophages could phagocytize Staphylococcus aureus Cowan I (protein A positive), but not S. aureus EMS (protein A negative) or Pseudomonas aeruginosa MN. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.2.504-509.1982
更新日期:1982-05-01 00:00:00
abstract::The roles of different ribonucleotide reductases (RNRs) in bacterial pathogenesis have not been studied systematically. In this work we analyzed the importance of the different Pseudomonas aeruginosa RNRs in pathogenesis using the Drosophila melanogaster host-pathogen interaction model. P. aeruginosa codes for three d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01212-10
更新日期:2011-07-01 00:00:00
abstract::The immunogenicity of the two major group 19 pneumococcal polysaccharides, types 19F and 19A, was studied in human volunteers. Antibody responses after vaccination with either 50 micrograms of type 19F polysaccharide, 50 micrograms of type 19A polysaccharide, or 25 micrograms of both polysaccharides premixed in a sing...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章
doi:10.1128/IAI.36.3.1261-1262.1982
更新日期:1982-06-01 00:00:00
abstract::The purpose of this study was to determine the effects of human monocytes, macrophages, and polymorphonuclear neutrophils (PMN) on the fungal pathogen Blastomyces dermatitidis in vitro. Peripheral blood monocyte monolayers significantly inhibited the replication of a virulent strain (V) and an avirulent strain (AV) of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.1.297-303.1982
更新日期:1982-04-01 00:00:00
abstract::Cimetidine is a powerful H2 receptor antagonist that eliminates histamine's effects on chemotaxis, phagocytosis, and superoxide anion production by phagocytes. The purpose of this study was to analyze the clinical and histopathological changes associated with experimental periodontitis in rabbits in response to topica...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.4.2402-2414.2006
更新日期:2006-04-01 00:00:00
abstract::Normal cell polarity protects epithelial cells against Pseudomonas aeruginosa invasion and cytotoxicity. Using epithelial cell clones with selective defects in sorting of membrane constituents, and using hepatocyte growth factor pretreatment, we found that polarized susceptibility to P. aeruginosa can be altered witho...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.7.3443-3446.1998
更新日期:1998-07-01 00:00:00
abstract::A model of invasive Haemophilus influenzae type b disease in rats was used to compare the net in vivo and in vitro multiplication rates of this bacterium. In both the blood of rats and in vitro cultures (fresh rat blood or enriched broth) there was an exponential increase in the number of CFU per milliliter with calcu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.3.911-913.1986
更新日期:1986-06-01 00:00:00