Abstract:
:The objective of these studies was to determine the role of macrophage inflammatory protein 1alpha/CCL3 in pulmonary host defense during Klebsiella pneumoniae infection. Following intratracheal inoculation, 7-day survival of CCL3(-/-) mice was less than 10%, compared to 60% for CCL3(+/+) mice. Survival of CCR5(-/-) mice was equivalent to that of controls, indicating that the enhanced susceptibility of CCL3(-/-) mice to K. pneumoniae is mediated via another CCL3 receptor, presumably CCR1. At day 3, CFU burden in the lungs of CCL3(-/-) mice was 800-fold higher than in CCL3(+/+) mice, demonstrating that CCL3 is critical for control of bacterial growth in the lung. Surprisingly, CCL3(-/-) mice had no differences in the recruitment of monocytes/macrophages and even showed enhanced neutrophil recruitment at days 1, 2, and 3 postinfection, compared to CCL3(+/+) mice. Therefore, the defect in clearance was not due to insufficient recruitment of leukocytes. No significant differences in cytokine levels of monocyte chemoattractant protein 1 (MCP-1), interleukin 12, gamma interferon, or tumor necrosis factor alpha in lung lavages were found between CCL3(+/+) and CCL3(-/-) mice. CCL3(-/-) alveolar macrophages were found to have significantly lower phagocytic activity toward K. pneumoniae than CCL3(+/+) alveolar macrophages. These findings demonstrate that CCL3 production is critical for activation of alveolar macrophages to control the pulmonary growth of the gram-negative bacterium K. pneumoniae.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Lindell DM,Standiford TJ,Mancuso P,Leshen ZJ,Huffnagle GBdoi
10.1128/IAI.69.10.6364-6369.2001keywords:
subject
Has Abstractpub_date
2001-10-01 00:00:00pages
6364-9issue
10eissn
0019-9567issn
1098-5522journal_volume
69pub_type
杂志文章abstract::Periodontitis is a bacterium-induced chronic inflammation that destroys tissues that attach teeth to jaw bone. Pathologically excessive matrix metalloproteinase 8 (MMP-8) is among the key players in periodontal destruction by initiating type I collagen degradation. We studied MMP-8 in Porphyromonas gingivalis-induced ...
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journal_title:Infection and immunity
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doi:10.1128/IAI.36.1.297-303.1982
更新日期:1982-04-01 00:00:00
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doi:10.1128/IAI.62.5.2119-2121.1994
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.65.11.4531-4538.1997
更新日期:1997-11-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.65.11.4877-4879.1997
更新日期:1997-11-01 00:00:00
abstract::Shigella flexneri 2a is an invasive enteric pathogen that may produce diarrhea when ingested by human beings and subhuman primates. We have previously shown that shigella diarrhea correlates with water and electrolyte transport abnormalities in the jejunum and colon. Dysentery alone is associated only with colonic tra...
journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1976-08-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1999-10-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1994-03-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1975-04-01 00:00:00
abstract::The kinetics of Bacillus anthracis toxin production in culture and its lethal activity in rats, mice, and guinea pigs were investigated. Lethal toxin activity was produced in vitro throughout exponential growth at essentially identical rates in both encapsulated virulent and nonencapsulated avirulent strains. The two ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.3.761-767.1984
更新日期:1984-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.7.1685-1691.1988
更新日期:1988-07-01 00:00:00
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doi:10.1128/IAI.05267-11
更新日期:2011-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.01262-06
更新日期:2007-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.10.5605-5612.2003
更新日期:2003-10-01 00:00:00
abstract::Type II heat-labile enterotoxin (LT-II) from Escherichia coli 41 was purified and compared with prototype LT-II encoded by genes from E. coli SA53. Both toxins were oligomeric proteins consisting of polypeptides A (Mr, 28,000) and B (Mr, 11,800). The A polypeptides were cleaved by trypsin into fragments A1 (Mr, 21,000...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.2.529-536.1986
更新日期:1986-11-01 00:00:00
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更新日期:2012-02-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.22.3.740-745.1978
更新日期:1978-12-01 00:00:00
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更新日期:1979-11-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2006-07-01 00:00:00
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更新日期:2008-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.9.2437-2442.1988
更新日期:1988-09-01 00:00:00
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journal_title:Infection and immunity
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doi:10.1128/IAI.66.6.2859-2865.1998
更新日期:1998-06-01 00:00:00
abstract::Endotoxin-treated mice exhibit a rapid rise in the level of the serum amyloid A (SAA) protein, but this effect is not observed in endotoxin-resistant C3H/HeJ mice. To evaluate the role of lymphoid cells in the production of SAA protein, C3H/HeJ mice were adoptively transfused with endotoxin-sensitive (C3H/HeN) bone ma...
journal_title:Infection and immunity
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更新日期:1979-01-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1999-06-01 00:00:00
abstract::An inhibitory factor from Actinobacillus actinomycetemcomitans Y4 was isolated, and its properties indicated that it was a bacteriocin (actinobacillicin). The bacteriocin was active against Streptococcus sanguis strains, Streptococcus uberis (FDC1), and Actinomyces viscosus T14 as well as other strains of A. actinomyc...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.686-691.1987
更新日期:1987-03-01 00:00:00
abstract::Urinary tract infection (UTI) is the second most common infection in humans, making it a global health priority. Nearly half of all women will experience a symptomatic UTI, with uropathogenic Escherichia coli (UPEC) being the major causative agent of the infection. Although there has been extensive research on UPEC vi...
journal_title:Infection and immunity
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更新日期:2018-02-20 00:00:00
abstract::Loss of the actin filament capping protein CapG has no apparent effect on the phenotype of mice maintained under sterile conditions; however, bone marrow-derived macrophages from CapG(-/-) mice exhibited distinct motility defects. We examined the ability of CapG(-/-) mice to clear two intracellular bacteria, Listeria ...
journal_title:Infection and immunity
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更新日期:2003-11-01 00:00:00