Peri-implant tissue is an important niche for Staphylococcus epidermidis in experimental biomaterial-associated infection in mice.

Abstract:

:Biomaterial-associated infections (BAI), which are predominantly caused by Staphylococcus epidermidis, are a significant problem in modern medicine. Biofilm formation is considered the pivotal element in the pathogenesis, but in previous mouse studies we retrieved S. epidermidis from peri-implant tissue. To assess the kinetics and generality of tissue colonization, we investigated BAI using two S. epidermidis strains, two biomaterials, and two mouse strains. With small inocula all implants were culture negative, whereas surrounding tissues were positive. When higher doses were used, tissues were culture positive more often than implants, with higher numbers of CFU. This was true for the different biomaterials tested, for both S. epidermidis strains, at different times, and for both mouse strains. S. epidermidis colocalized with host cells at a distance that was >10 cell layers from the biomaterial-tissue interface. We concluded that in mouse experimental BAI S. epidermidis peri-implant tissue colonization is more important than biofilm formation.

journal_name

Infect Immun

journal_title

Infection and immunity

authors

Broekhuizen CA,de Boer L,Schipper K,Jones CD,Quadir S,Feldman RG,Dankert J,Vandenbroucke-Grauls CM,Weening JJ,Zaat SA

doi

10.1128/IAI.01262-06

subject

Has Abstract

pub_date

2007-03-01 00:00:00

pages

1129-36

issue

3

eissn

0019-9567

issn

1098-5522

pii

IAI.01262-06

journal_volume

75

pub_type

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