Abstract:
:It has been reported that Neisseria gonorrhoeae possesses a very high level of catalase activity, but the regulation of catalase expression has not been investigated extensively. In Escherichia coli and Salmonella enterica serovar Typhimurium, it has been demonstrated that OxyR is a positive regulator of hydrogen peroxide-inducible genes, including the gene encoding catalase. The oxyR gene from N. gonorrhoeae was cloned and used to complement an E. coli oxyR mutant, confirming its identity and function. The gene was inactivated by inserting a kanamycin resistance cassette and used to make a knockout allele on the chromosome of N. gonorrhoeae strain 1291. In contrast to E. coli, the N. gonorrhoeae oxyR::kan mutant expressed ninefold-more catalase activity and was more resistant to hydrogen peroxide killing than the wild type. These data are consistent with OxyR in N. gonorrhoeae acting as a repressor of catalase expression.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Tseng HJ,McEwan AG,Apicella MA,Jennings MPdoi
10.1128/iai.71.1.550-556.2003keywords:
subject
Has Abstractpub_date
2003-01-01 00:00:00pages
550-6issue
1eissn
0019-9567issn
1098-5522journal_volume
71pub_type
杂志文章abstract::Protozoan parasites of the genus Leishmania exist as obligatory intracellular amastigotes and invade macrophages and Langerhans cells, the dendritic cells of the skin. Langerhans cells are much more efficient in presenting Leishmania major antigen to T cells than macrophages are and have the unique ability to retain p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.8.3444-3450.1997
更新日期:1997-08-01 00:00:00
abstract::Detoxified mutants of the Escherichia coli heat-labile toxin (LT) act as mucosal adjuvants to intranasally presented coadministered antigens. Here, we compare the adjuvant activity of a panel of detoxified derivatives of LT, using both intranasal (i.n.) and oral (p.o.) routes of administration. The mutants used as adj...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.9.4400-4406.1999
更新日期:1999-09-01 00:00:00
abstract::The diarrheagenic pathogen enteropathogenic Escherichia coli (EPEC) is responsible for significant infant mortality and morbidity, particularly in developing countries. EPEC pathogenesis relies on a type III secretion system-mediated transfer of virulence effectors into host cells. EPEC modulates host cell survival an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01690-06
更新日期:2007-05-01 00:00:00
abstract::Mouse fibroblasts (L cells) infected with the 6BC strain of Chlamydia psittaci released potassium ion (K(+)) into the extracellular milieu in a way that depended on size of inoculum and time after infection. When the multiplicity of infection was 500 to 1,000 50% infectious units (ID(50)) per L cell, loss of intracell...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.827-832.1978
更新日期:1978-03-01 00:00:00
abstract::The susceptibility of HeLa cells to Mycoplasma pneumoniae-induced injury was examined. Infections were initiated with relatively low mycoplasma doses, carried out in a culture medium incapable of supporting M. pneumoniae replication in the absence of host cells, and monitored for up to 10 days. Under these conditions,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.8.2054-2059.1988
更新日期:1988-08-01 00:00:00
abstract::An immunogenic aminopeptidase was purified from Brucella melitensis strain VTRM1. The purification procedure consisted of ammonium sulfate fractionation and three chromatographic steps. This procedure resulted in a yield of 29% and a 144-fold increase in specific activity. The aminopeptidase appeared to be a monomeric...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.9.5238-5244.2003
更新日期:2003-09-01 00:00:00
abstract::Once in the cytoplasm of mammalian cells, Shigella flexneri expresses a motile phenotype caused by polar directional assembly of actin. This process depends on accumulation of IcsA (VirG), a 120-kDa protein with ATPase activity, at the pole of the bacterium opposite to that at which ongoing septation occurs. IcsA is a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.2.511-517.1996
更新日期:1996-02-01 00:00:00
abstract::Vibrio cholerae is a monoflagellated gram-negative bacterium that causes the severe diarrheal disease cholera. In contrast to Salmonella enterica serovar Typhimurium infection, which is accompanied by both acute diarrhea and high-level inflammation, V. cholerae infection is largely noninflammatory in human hosts. Bact...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00736-07
更新日期:2008-03-01 00:00:00
abstract::Human multidrug efflux transporters are known for their ability to extrude antibiotics and toxic compounds out of cells, yet accumulating data indicate they have additional functions in diverse physiological processes not related to drug efflux. Here, we show that the human multidrug transporter P-glycoprotein (P-gp) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00380-15
更新日期:2015-06-01 00:00:00
abstract::Identification of antigenically conserved surface components of Haemophilus ducreyi may facilitate the development of reagents to diagnose and prevent chancroid. A hybridoma derived from a mouse immunized with nontypeable Haemophilus influenzae produced a monoclonal antibody (MAb), designated 3B9, that bound to 35 of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.2.385-391.1992
更新日期:1992-02-01 00:00:00
abstract::The Yersinia high-pathogenicity island (HPI) is common to multiple virulence strategies used by Escherichia coli strains associated with urinary tract infection (UTI). Among the genes in this island are ybtP and ybtQ, encoding distinctive ATP binding cassette (ABC) proteins associated with iron(III)-yersiniabactin imp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01211-15
更新日期:2016-04-22 00:00:00
abstract::Using polystyrene microspheres coated with heparin or heparan sulfate, it was shown that coated microspheres specifically bound eukaryotic cells and were endocytosed by nonprofessional phagocytic cells. Coated microspheres displayed properties of binding to eukaryotic cells that were similar to those of chlamydiae, an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1080-1085.2000
更新日期:2000-03-01 00:00:00
abstract::The Lyme disease agent, Borrelia burgdorferi, is able to persistently infect humans and animals for months or years in the presence of an active immune response. It is not known how the organisms survive immune attack in the mammalian host. vlsE, a gene localized near one end of linear plasmid lp28-1 and encoding a su...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.8.3689-3697.1998
更新日期:1998-08-01 00:00:00
abstract::We have previously shown that splenocytes from mice acutely infected with Trypanosoma cruzi exhibit high levels of nitric oxide (NO)-mediated apoptosis. In the present study, we used the gamma interferon (IFN-gamma)-knockout (IFN-gamma(-/-)) mice to investigate the role of IFN-gamma in modulating apoptosis induction a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.8.3864-3871.1999
更新日期:1999-08-01 00:00:00
abstract::Neisseria gonorrhoeae, the etiologic agent of gonorrhea, is frequently asymptomatic in women, often leading to chronic infections. One factor contributing to this may be biofilm formation. N. gonorrhoeae can form biofilms on glass and plastic surfaces. There is also evidence that biofilm formation may occur during nat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00036-09
更新日期:2009-09-01 00:00:00
abstract::Three antigenic determinants were revealed in H3 hemagglutinin of influenza A viruses isolated from 1968 to 1975. One of them was common for all viruses, and two others specified differences between the viruses possessing H3 hemagglutinin. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.804-807.1979
更新日期:1979-06-01 00:00:00
abstract::This report describes an attempt to define the factors which incite secondary bacterial pneumonias. Groups of mice were given bacteria intraperitoneally and, at various intervals, Newcastle disease virus intravenously. There was an increase in the number of deaths and in the rates of death in these groups, when compar...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.3.488-493.1971
更新日期:1971-03-01 00:00:00
abstract::Protection against colibacillosis in neonatal piglets was obtained by immunization of pregnant dams with procholeragenoid. Procholeragenoid is a stable high-molecular-weight aggregate of cholera toxin formed during the heating of cholera toxin. Procholeragenoid retained approximately 1% of the toxicity of native toxin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.35.3.887-894.1982
更新日期:1982-03-01 00:00:00
abstract::The adherence of Plasmodium falciparum-infected red blood cells (IRBCs) in the human placenta is mediated by chondroitin-4-sulfate (C4S). Although IRBC binding to C4S has been unequivocally established, the adherence characteristics of IRBCs at different stages of parasite development and through successive parasite g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00478-07
更新日期:2007-09-01 00:00:00
abstract::Saccharomyces boulardii, a nonpathogenic yeast, is effective in treating some patients with Clostridium difficile diarrhea and colitis. We have previously reported that S. boulardii inhibits rat ileal secretion in response to C. difficile toxin A possibly by releasing a protease that digests the intestinal receptor fo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.12.5225-5232.1996
更新日期:1996-12-01 00:00:00
abstract::Previous work in our laboratory has shown that corticosterone increases the susceptibility of macrophages from Bcgs mice to the growth of Mycobacterium avium. The innate antimycobacterial activity of macrophages from Bcgr mice was not affected by corticosterone. In contrast to the differential effect of corticosterone...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.8.2983-2988.1995
更新日期:1995-08-01 00:00:00
abstract::Nonimmune binding of immunoglobulin to whole bacteria was quantitated for North American isolates of Haemophilus somnus recovered from cattle with pneumonia, reproductive failure (abortion), or thromboembolic meningoencephalitis or from the vagina or prepuce of carrier cattle. Quantitative binding activity covered a w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.2.639-642.1989
更新日期:1989-02-01 00:00:00
abstract::The scrub typhus 58-kilodalton (kDa) antigen (Sta58) of Rickettsia tsutsugamushi is a major protein antigen often recognized by humans infected with scrub typhus rickettsiae. A 2.9-kilobase HindIII fragment containing a complete sta58 gene was cloned in Escherichia coli and found to express the entire Sta58 antigen an...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.5.1360-1368.1990
更新日期:1990-05-01 00:00:00
abstract::Helicobacter pylori colonizes the gastric epithelium of at least 50% of the world's human population, playing a causative role in the development of chronic gastritis, peptic ulcers, and gastric adenocarcinoma. Current evidence indicates that H. pylori can invade epithelial cells in the gastric mucosa. However, relati...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01085-06
更新日期:2006-12-01 00:00:00
abstract::Pregnancy-associated malaria is a severe clinical syndrome associated with the sequestration of Plasmodium falciparum-infected erythrocytes in the placenta. Placental binding is mediated by VAR2CSA, a member of the large and diverse P. falciparum erythrocyte membrane 1 (PfEMP1) protein family. To better understand if ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00410-09
更新日期:2010-05-01 00:00:00
abstract::In mice infected with virulent blood (trypomastigote) forms of Trypanosoma cruzi, complement depletion with cobra venom factor caused a marked exacerbation of the disease evidenced by significantly increased parasitemia levels and early mortality as compared with those of untreated infected animals. The effect was gre...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.1.86-91.1975
更新日期:1975-01-01 00:00:00
abstract::Mycobacterial growth inhibitory factor (MycoIF), found in supernatant fluids of mouse spleen cell cultures that have been stimulated in vitro with homologous antigen, inhibited the intracellular multiplication of virulent tubercle bacilli within normal mouse peritoneal macrophages in vitro. Antigenically stimulated H...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.4.833-840.1975
更新日期:1975-10-01 00:00:00
abstract::A hallmark of Bartonella henselae is persistent bacteremia in cats despite the presence of a vigorous host immune response. To understand better the long-term survival of B. henselae in cats, we examined the feline humoral immune response to B. henselae outer membrane (OM) proteins in naturally and experimentally infe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.6.3097-3105.2004
更新日期:2004-06-01 00:00:00
abstract::Previous studies have indicated that the ability to bind to fibronectin is a key feature in successful cell invasion by Campylobacter jejuni. Given the spatial distribution of fibronectin and the architecture of the epithelium, this suggests the possibility that C. jejuni cell invasion might preferentially occur at th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.12.6665-6671.2002
更新日期:2002-12-01 00:00:00
abstract::Porphyromonas gingivalis, a periodontopathic bacterium, is known to invade oral epithelial cells in periodontal lesions, although the mechanism is unclear. In the present study, goat polyclonal anti-intercellular adhesion molecule 1 (anti-ICAM-1) antibody inhibited the invasion of P. gingivalis into KB cells (human or...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6290-6298.2005
更新日期:2005-10-01 00:00:00