Salivary, nasal, genital, and systemic antibody responses in monkeys immunized intranasally with a bacterial protein antigen and the Cholera toxin B subunit.


:Previous attempts to induce mucosal antibodies in rhesus monkeys by enteric immunization have resulted in only modest and short-lived responses, dominated by immunoglobulin M (IgM) antibodies in the plasma. In this study, two groups of rhesus monkeys were immunized intranasally three times at 2-week intervals with a bacterial protein antigen (AgI/II) either chemically coupled to or mixed with the B subunit of cholera toxin (CT), a known potent mucosal immunogen and carrier for other immunogens. Cells secreting antibodies, predominantly of the IgA isotype, to AgI/II and to CT were detected in the peripheral blood 1 week after each immunization, indicating the dissemination of IgA-secreting precursor cells through the mucosal immune system. IgG and, to a lesser extent, IgA antibodies to both proteins were induced in the plasma commencing after the second immunization. Plasma IgE concentrations and IgE antibody levels were not consistently raised during the immunization period. IgA antibodies were found in nasal and vaginal washes. Nasal IgG but not IgA antibodies showed a significant positive correlation with plasma IgG antibody levels, suggesting that they were largely derived by transudation from the circulation. Analysis of the molecular form of vaginal IgA indicated that both secretory and monomeric forms of IgA were present in various proportions. Furthermore, neither IgG nor IgA antibodies in vaginal washes were correlated with plasma antibody responses, suggesting the contribution of locally synthesized antibodies of both isotypes. Comparison of the responses between the two groups of animals showed only sporadic significant differences, indicating that intranasal immunization with AgI/II either coupled to or mixed with the B subunit of CT was equally effective at inducing generalized IgA antibody responses in the mucosal immune system and predominantly IgG antibodies in the plasma.


Infect Immun


Infection and immunity


Russell MW,Moldoveanu Z,White PL,Sibert GJ,Mestecky J,Michalek S M




Has Abstract


1996-04-01 00:00:00












  • Antibody Response to Lyme Disease Spirochetes in the Context of VlsE-Mediated Immune Evasion.

    abstract::Lyme disease (LD), the most prevalent tick-borne illness in North America, is caused by Borrelia burgdorferi The long-term survival of B. burgdorferi spirochetes in the mammalian host is achieved though VlsE-mediated antigenic variation. It is mathematically predicted that a highly variable surface antigen prolongs ba...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Rogovskyy AS,Gillis DC,Ionov Y,Gerasimov E,Zelikovsky A

    更新日期:2016-12-29 00:00:00

  • Induction of enhanced resistance against encephalomyocarditis virus infection of mice by nonviable Mycobacterium tuberculosis: mechanisms of protection.

    abstract::Nonviable Mycobacterium tuberculosis strain Jamaica suspended in oil-droplet emulsions was used to enhance resistance of mice against encephalomyocarditis virus (EMCV). The mycobacteria-injected mice were significantly resistant to 50,000 50% lethal doses of EMCV. Similar concentrations of virus in plasma of normal an...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lodmell DL,Ewalt LC

    更新日期:1978-12-01 00:00:00

  • Predominant gram-positive bacteria in human feces: numbers, variety, and persistence.

    abstract::The predominant gram-positive bacteria in 47 fecal specimens from 10 healthy men were studied by microscopic and cultural counts, by the characterization and tentative identification of isolates, and by the use of fluorescein isothiocyanate (FITC)-conjugated globulins prepared using some of the isolates. Gram-positive...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Gossling J,Slack JM

    更新日期:1974-04-01 00:00:00

  • Detrimental role of CC chemokine receptor 4 in murine polymicrobial sepsis.

    abstract::CC chemokine receptor 4 (CCR4) and its two ligands, CCL17 and CCL22, are critically involved in different immune processes. In models of lipopolysaccharide-induced shock, CCR4-deficient (CCR4(-/-)) mice showed improved survival rates associated with attenuated proinflammatory cytokine release. Using CCR4(-/-) mice wit...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Traeger T,Kessler W,Assfalg V,Cziupka K,Koerner P,Dassow C,Breitbach K,Mikulcak M,Steinmetz I,Pfeffer K,Heidecke CD,Maier S

    更新日期:2008-11-01 00:00:00

  • Biphasic effects of muramyl dipeptide or lipopolysaccharide on superoxide anion-generating activities of macrophages.

    abstract::The superoxide anion (O2-)-generating activity of guinea pig macrophages stimulated by wheat germ agglutinin (WGA), immune complexes, or phorbol myristate acetate (PMA) was studied after short- and long-term exposures of the cells to muramyl dipeptide (MDP) or lipopolysaccharide (LPS). Neither MDP nor LPS alone induce...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Yagawa K,Kaku M,Ichinose Y,Nagao S,Tanaka A,Tomoda A

    更新日期:1984-07-01 00:00:00

  • Bacteroides fragilis: a possible cause of acute diarrheal disease in newborn lambs.

    abstract::Bacteroides fragilis with enterotoxin-like activity (BFEL) was isolated from the feces of 24- to 48-h-old lambs with acute diarrheal disease on three different sheep ranches in the Northern Rocky Mountain region of the United States. The lamb intestinal loop test was used to enrich for the bacterium before its initial...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Myers LL,Firehammer BD,Shoop DS,Border MM

    更新日期:1984-05-01 00:00:00

  • An Arcanobacterium (Actinomyces) pyogenes mutant deficient in production of the pore-forming cytolysin pyolysin has reduced virulence.

    abstract::Pyolysin (PLO), the hemolytic exotoxin expressed by Arcanobacterium (Actinomyces) pyogenes, is a member of the thiol-activated cytolysin family of bacterial toxins. Insertional inactivation of the plo gene results in loss of expression of PLO with a concomitant loss in hemolytic activity. The plo mutant, PLO-1, has an...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Jost BH,Songer JG,Billington SJ

    更新日期:1999-04-01 00:00:00

  • Plasmid-dependent attachment of Agrobacterium tumefaciens to plant tissue culture cells.

    abstract::Kinetic, microscopic, and biochemical studies show that virulent Ti (tumor inducing)-plasmid-containing strains of Agrobacterium attach to normal tobacco and carrot tissue culture cells. Kinetic studies showed that virulent strains of A. tumefaciens attach to the plant tissue culture cells in increasing numbers during...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Matthysse AG,Wyman PM,Holmes KV

    更新日期:1978-11-01 00:00:00

  • Effects of tumor necrosis factor alpha on dendritic cell accumulation in lymph nodes draining the immunization site and the impact on the anticryptococcal cell-mediated immune response.

    abstract::Cell-mediated immune (CMI) responses and tumor necrosis factor alpha (TNF-alpha) have been shown to be essential in acquired protection against Cryptococcus neoformans. Induction of a protective anticryptococcal CMI response includes increases in dendritic cells (DC) and activated CD4(+) T cells in draining lymph node...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Bauman SK,Huffnagle GB,Murphy JW

    更新日期:2003-01-01 00:00:00

  • Local and systemic antibody responses in mice immunized intranasally with native and detergent-extracted outer membrane vesicles from Neisseria meningitidis.

    abstract::The mouse humoral immune response toward native or detergent-extracted outer membrane vesicles (NOMVs and DOMVs, respectively) from Neisseria meningitidis was determined after intranasal immunization. Both preparations elicited high frequencies of NOMV-specific antibody-forming cells (AFCs) locally in the nasal associ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Guthrie T,Wong SY,Liang B,Hyland L,Hou S,Høiby EA,Andersen SR

    更新日期:2004-05-01 00:00:00

  • Nonhemolytic Listeria monocytogenes mutants that are also noninvasive for mammalian cells in culture: evidence for coordinate regulation of virulence.

    abstract::We identified nonhemolytic mutants of Listeria monocytogenes that were severely deficient in their ability to invade mammalian nonprofessional phagocytes. These mutants were generated spontaneously or by means of transposon Tn916 mutagenesis. In terms of their extracellular proteins, the noninvasive mutants were defic...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kathariou S,Pine L,George V,Carlone GM,Holloway BP

    更新日期:1990-12-01 00:00:00

  • Detection of specific antibody in adult human periodontitis sera to surface antigens of Bacteroides gingivalis.

    abstract::Sera from adult periodontitis patients were analyzed for antibody activity against surface antigens of Bacteroides gingivalis by immunoblotting analysis. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Naito Y,Okuda K,Takazoe I

    更新日期:1987-03-01 00:00:00

  • Nontypeable Haemophilus influenzae strain 2019 produces a biofilm containing N-acetylneuraminic acid that may mimic sialylated O-linked glycans.

    abstract::Previous studies suggested that nontypeable Haemophilus influenzae (NTHI) can form biofilms during human and chinchilla middle ear infections. Microscopic analysis of a 5-day biofilm of NTHI strain 2019 grown in a continuous-flow chamber revealed that the biofilm had a diffuse matrix interlaced with multiple water cha...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Greiner LL,Watanabe H,Phillips NJ,Shao J,Morgan A,Zaleski A,Gibson BW,Apicella MA

    更新日期:2004-07-01 00:00:00

  • Identification of lipoprotein homologues of pneumococcal PsaA in the equine pathogens Streptococcus equi and Streptococcus zooepidemicus.

    abstract::Streptococcus equi and Streptococcus zooepidemicus are major etiological agents of upper and lower airway disease in horses. Despite the considerable animal suffering and economic burden associated with these diseases, the factors that contribute to the virulence of these equine pathogens have not been extensively inv...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Harrington DJ,Greated JS,Chanter N,Sutcliffe IC

    更新日期:2000-10-01 00:00:00

  • Effect of oxygen-dependent antimicrobial systems on Legionella pneumophila.

    abstract::Legionella pneumophila was susceptible to the antimicrobial action of oxygen metabolites generated by both the myeloperoxidase-H(2)O(2)-halide and the xanthine oxidase systems. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lochner JE,Friedman RL,Bigley RH,Iglewski BH

    更新日期:1983-01-01 00:00:00

  • Mouse model of hemolytic-uremic syndrome caused by endotoxin-free Shiga toxin 2 (Stx2) and protection from lethal outcome by anti-Stx2 antibody.

    abstract::Hemolytic-uremic syndrome (HUS) results from infection by Shiga toxin (Stx)-producing Escherichia coli and is the most common cause of acute renal failure in children. We have developed a mouse model of HUS by administering endotoxin-free Stx2 in multiple doses over 7 to 8 days. At sacrifice, moribund animals demonstr...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Sauter KA,Melton-Celsa AR,Larkin K,Troxell ML,O'Brien AD,Magun BE

    更新日期:2008-10-01 00:00:00

  • Intraspleen delivery of a DNA vaccine coding for superoxide dismutase (SOD) of Brucella abortus induces SOD-specific CD4+ and CD8+ T cells.

    abstract::In the development of vaccines capable of providing immunity against brucellosis, Cu-Zn superoxide dismutase (SOD) has been demonstrated to be one of the protective immunogens of Brucella abortus. In an earlier study, we provided strong evidence that intramuscular injection with a plasmid DNA carrying the SOD gene (pc...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Muñoz-Montesino C,Andrews E,Rivers R,González-Smith A,Moraga-Cid G,Folch H,Céspedes S,Oñate AA

    更新日期:2004-04-01 00:00:00

  • Cryptococcal yeast cells invade the central nervous system via transcellular penetration of the blood-brain barrier.

    abstract::Cryptococcal meningoencephalitis develops as a result of hematogenous dissemination of inhaled Cryptococcus neoformans from the lung to the brain. The mechanism(s) by which C. neoformans crosses the blood-brain barrier (BBB) is a key unresolved issue in cryptococcosis. We used both an in vivo mouse model and an in vit...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Chang YC,Stins MF,McCaffery MJ,Miller GF,Pare DR,Dam T,Paul-Satyaseela M,Kim KS,Kwon-Chung KJ

    更新日期:2004-09-01 00:00:00

  • Membrane glycoprotein M-2 protects against Leishmania amazonensis infection.

    abstract::Previous passive antibody transfer experiments have indicated that immunity to a 46-kilodalton membrane glycoprotein (M-2) of Leishmania amazonensis may protect against infection with this parasite. In the studies described in this paper, we investigated the ability of the purified M-2 molecule to elicit a protective ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Champsi J,McMahon-Pratt D

    更新日期:1988-12-01 00:00:00

  • Isolation of Neisseria gonorrhoeae mutants that show enhanced trafficking across polarized T84 epithelial monolayers.

    abstract::Initiation of a gonococcal infection involves attachment of Neisseria gonorrhoeae to the plasma membrane of an epithelial cell in the mucosal epithelium and its internalization, transepithelial trafficking, and exocytosis from the basal membrane. Piliation and expression of certain Opa proteins and the immunoglobulin ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Hopper S,Wilbur JS,Vasquez BL,Larson J,Clary S,Mehr IJ,Seifert HS,So M

    更新日期:2000-02-01 00:00:00

  • Inhibition by Mycoplasma dispar of ciliary activity in tracheal organ cultures.

    abstract::The inhibition by Mycoplasma dispar of ciliary activity of organ cultures of bovine fetal trachea was found to be dependent on the presence of serum in the maintenance medium. Inhibitory activity was unaffected by catalase and no toxic substance was demonstrated in organ culture supernatant fluid. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Howard CJ,Thomas LH

    更新日期:1974-08-01 00:00:00

  • Effect of zinc chloride on hamster lymphoid cells: mitogenicity and differential enhancement of lipopolysaccharide stimulation of lymphocytes.

    abstract::ZnCl2 over a very narrow concentration range was found to be mitogenic for hamster lymph node cells but not for thymocytes or splenocytes. Maximal leucine, [3H]uridine, and [3H]thymidine incorporation. Addition of 10 micron ZnCl2 was found to greatly enhance the stimulation observed with the B-lymphocyte mitogen lipop...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Hart DA

    更新日期:1978-02-01 00:00:00

  • The fibronectin-binding protein Fnm contributes to adherence to extracellular matrix components and virulence of Enterococcus faecium.

    abstract::The interaction between bacteria and fibronectin is believed to play an important role in the pathogenicity of clinically important Gram-positive cocci. In the present study, we identified a gene encoding a predicted fibronectin-binding protein of Enterococcus faecium (fnm), a homologue of Streptococcus pneumoniae pav...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Somarajan SR,La Rosa SL,Singh KV,Roh JH,Höök M,Murray BE

    更新日期:2015-12-01 00:00:00

  • Regulation of Trypanosoma cruzi infection in mice by gamma interferon and interleukin 10: role of NK cells.

    abstract::Gamma interferon (IFN-gamma) plays an important role in experimental Trypanosoma cruzi infections, presumably by controlling the early replication of parasites in host macrophages. In this work, we show that NK cells represent an important cell type responsible for the production of most of the IFN-gamma in the early ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Cardillo F,Voltarelli JC,Reed SG,Silva JS

    更新日期:1996-01-01 00:00:00

  • Differences in phagocytosis and killing by alveolar macrophages from humans, rabbits, rats, and hamsters.

    abstract::Phagocytosis and killing by alveolar macrophages from humans, rabbits, rats, and hamsters, were compared in vitro. In the absence of serum opsonins, human alveolar macrophages could phagocytize Staphylococcus aureus Cowan I (protein A positive), but not S. aureus EMS (protein A negative) or Pseudomonas aeruginosa MN. ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Nguyen BY,Peterson PK,Verbrugh HA,Quie PG,Hoidal JR

    更新日期:1982-05-01 00:00:00

  • Protective capacity of antibodies against Escherichia coli and K antigens.

    abstract::Antibodies to Escherichia coli O and K antigens were raised in rabbits by repeated immunizations with whole, Formalin-killed and, later, liver bacteria. The serum antibody levels were determined with the ammonium sulfate precipitation technique after radioiodinating the antigens. The K antigens had to be conjugated to...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Kaijser B,Ahlstedt S

    更新日期:1977-08-01 00:00:00

  • Amyloidosis and the serum amyloid A protein response to muramyl dipeptide analogs and different mycobacterial species.

    abstract::Serum amyloid A protein (SAA) elevation accompanies induction of secondary amyloidosis in mice given Mycobacterium butyricum in Freund adjuvant. The synthesis of SAA by cultured hepatocytes is induced by a macrophage-derived mediator, which has been identified as interleukin 1. In these studies, SAA synthesis has been...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: McAdam KP,Foss NT,Garcia C,DeLellis R,Chedid L,Rees RJ,Wolff SM

    更新日期:1983-03-01 00:00:00

  • Critical role of neutrophils in eliminating Listeria monocytogenes from the central nervous system during experimental murine listeriosis.

    abstract::Neutrophils are the main inflammatory cell present in lesions involving the central nervous system (CNS) during human and murine listeriosis. In this study, administration of the neutrophil-depleting monoclonal antibody RB6-8C5 during experimental murine listeriosis facilitated the multiplication of Listeria monocytog...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: López S,Marco AJ,Prats N,Czuprynski CJ

    更新日期:2000-08-01 00:00:00

  • Antihistoplasma effect of activated mouse splenic macrophages involves production of reactive nitrogen intermediates.

    abstract::The mechanism by which recombinant murine gamma interferon (rMuIFN-gamma) and bacterial lipopolysaccharide (LPS) activate mouse resident splenic macrophages to inhibit the intracellular growth of the fungus Histoplasma capsulatum was examined. Growth inhibition depended on L-arginine metabolism. The growth inhibitory ...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Lane TE,Wu-Hsieh BA,Howard DH

    更新日期:1994-05-01 00:00:00

  • Potential role of autoantibodies in the regulation of cytokine responses during bacterial infections.

    abstract::An immunoregulatory mechanism involving release of neutralizing autoantibodies (Aabs) to self cytokines during bacterial infections is presented herein. Intraperitoneal inoculation of Haemophilus influenzae type b into Sprague-Dawley rats resulted in a self-limiting meningitis. High levels of cells expressing mRNA for...

    journal_title:Infection and immunity

    pub_type: 杂志文章


    authors: Bakhiet M,Diab A,Mahamustafa,Jiezhu,Lindqvist L,Link H

    更新日期:1997-08-01 00:00:00