Abstract:
:We have previously demonstrated the existence of Klebsiella pneumoniae clinical isolates deficient in the lipopolysaccharide O side chain, the major factor for resistance to complement-mediated killing in this bacterial species. These isolates are complement resistant, and their mechanisms to resist complement were investigated by selecting transposon-generated complement-sensitive mutants. One mutant with a drastically reduced capacity to grow in nonimmune human serum carried the transposon inserted in an open reading frame of a gene cluster involved in capsule synthesis. This mutant produced less capsule, bound more molecules of the complement component C3, and was more sensitive to complement-mediated and opsonophagocytic killings than was the parent strain. Four additional clinical isolates representing four different K serotypes were studied, and results showed that capsular polysaccharide is a major complement resistance factor in these O side chain-deficient isolates.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Alvarez D,Merino S,Tomás JM,Benedí VJ,Albertí Sdoi
10.1128/iai.68.2.953-955.2000keywords:
subject
Has Abstractpub_date
2000-02-01 00:00:00pages
953-5issue
2eissn
0019-9567issn
1098-5522journal_volume
68pub_type
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