Abstract:
:Group A streptococci (GAS) are highly prevalent human pathogens whose primary ecological niche is the superficial epithelial layers of the throat and/or skin. Many GAS strains with a strong tendency to cause pharyngitis are distinct from strains that tend to cause impetigo; thus, genetic differences between them may confer host tissue-specific virulence. In this study, the FbaA surface protein gene was found to be present in most skin specialist strains but largely absent from a genetically related subset of pharyngitis isolates. In an ΔfbaA mutant constructed in the impetigo strain Alab49, loss of FbaA resulted in a slight but significant decrease in GAS fitness in a humanized mouse model of impetigo; the ΔfbaA mutant also exhibited decreased survival in whole human blood due to phagocytosis. In assays with highly sensitive outcome measures, Alab49ΔfbaA was compared to other isogenic mutants lacking virulence genes known to be disproportionately associated with classical skin strains. FbaA and PAM (i.e., the M53 protein) had additive effects in promoting GAS survival in whole blood. The pilus adhesin tip protein Cpa promoted Alab49 survival in whole blood and appears to fully account for the antiphagocytic effect attributable to pili. The finding that numerous skin strain-associated virulence factors make slight but significant contributions to virulence underscores the incremental contributions to fitness of individual surface protein genes and the multifactorial nature of GAS-host interactions.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Rouchon CN,Ly AT,Noto JP,Luo F,Lizano S,Bessen DEdoi
10.1128/IAI.00374-17subject
Has Abstractpub_date
2017-10-18 00:00:00issue
11eissn
0019-9567issn
1098-5522pii
IAI.00374-17journal_volume
85pub_type
杂志文章abstract::We have developed an in vitro assay to study the intramacrophage replication of Mycobacterium tuberculosis or Mycobacterium bovis BCG. In this assay, extracellular mycobacterial replication was avoided by using a small infecting inoculum and a new method for efficiently removing nonphagocytocytized bacilli. The abilit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.2.786-793.1982
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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pub_type: 杂志文章
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更新日期:2008-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1975-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.6.1986-1991.1990
更新日期:1990-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1976-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1993-06-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1990-10-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1985-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1986-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.3.804-807.1979
更新日期:1979-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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