Abstract:
:Invasive aspergillosis is a major threat for patients suffering from chronic granulomatous disease (CGD). Although Aspergillus fumigatus is the most commonly encountered Aspergillus species, the presence of A. nidulans appears to be disproportionately high in CGD patients. The purpose of this study was to investigate the involvement of the NADPH oxidase and the resulting reactive oxygen species (ROS) in host defense against fungi and to clarify their relationship toward A. nidulans. Murine CGD alveolar macrophages (AM) and polymorphonuclear leukocytes (PMN) and peripheral blood mononuclear cells (PBMC) from healthy controls and CGD patients were challenged with either A. fumigatus or A. nidulans. Analysis of the antifungal effects of ROS revealed that A. nidulans, in contrast to A. fumigatus, is not susceptible to ROS. In addition, infection with live A. nidulans did not result in any measurable ROS release. Remarkably, human CGD PMN and PBMC and murine CGD AM were at least equipotent at arresting conidial germination compared to healthy controls. Blocking of the NADPH oxidase resulted in significantly reduced damage of A. fumigatus but did not affect A. nidulans hyphae. Furthermore, the microbicidal activity of CGD PMN was maintained toward A. nidulans but not A. fumigatus. In summary, antifungal resistance to A. nidulans is not directly ROS related. The etiology of A. nidulans infections in CGD cannot be explained by the simple absence of the direct microbicidal effect of ROS. In vivo, the NADPH oxidase is a critical regulator of innate immunity whose unraveling will improve our understanding of fungal pathogenesis in CGD.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Henriet SS,Hermans PW,Verweij PE,Simonetti E,Holland SM,Sugui JA,Kwon-Chung KJ,Warris Adoi
10.1128/IAI.00921-10subject
Has Abstractpub_date
2011-02-01 00:00:00pages
767-73issue
2eissn
0019-9567issn
1098-5522pii
IAI.00921-10journal_volume
79pub_type
杂志文章abstract::Bile aspirated from chicken gall bladders was found to contain substances neutralizing Newcastle disease virus (NDV). Nonspecific factors were present in the bile, probably the bile acids, which caused a reduction in the infectivity of the virus. Specific anti-NDV activity was found in the bile of birds that had been ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.11.4.692-697.1975
更新日期:1975-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.16.1.213-217.1977
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.11.4629-4636.1993
更新日期:1993-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.8.2535-2541.1990
更新日期:1990-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1998-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2001-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2002-12-01 00:00:00
abstract::Group A streptococcal cell walls inhibited phagocytosis of polystyrene latex particles by rat peritoneal macrophages in vitro. This inhibition was not accompanied by a measurable loss of cell viability. Group D streptococcal cell walls were relatively ineffective in altering phagocytosis. The effectiveness of group A ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.1.258-261.1978
更新日期:1978-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.3.1112-1118.1982
更新日期:1982-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.5.2803-2809.2004
更新日期:2004-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2081-2087.2004
更新日期:2004-04-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4011-4016.1997
更新日期:1997-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.3.818-822.1986
更新日期:1986-06-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.74.1.265-272.2006
更新日期:2006-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00078-07
更新日期:2007-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.47.1.112-117.1985
更新日期:1985-01-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.8.3576-3579.1994
更新日期:1994-08-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.1.190-197.1985
更新日期:1985-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.00533-10
更新日期:2010-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.33.1.322-325.1981
更新日期:1981-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.3.1424-1431.1999
更新日期:1999-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.825-827.1987
更新日期:1987-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.4.3.256-263.1971
更新日期:1971-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章,评审
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更新日期:2010-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1113-1120.2002
更新日期:2002-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00513-10
更新日期:2010-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.10.3418-3423.1991
更新日期:1991-10-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00123-06
更新日期:2006-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.1.177-184.2002
更新日期:2002-01-01 00:00:00
abstract::With large amounts of parasite gene sequence available, additional bioinformatic tools to screen these sequences for identifying genes encoding antigens are needed. Proteins containing tandem repeat (TR) domains are often B-cell antigens, and antibody responses toward TR domains of the proteins are dominant in human i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01205-06
更新日期:2007-02-01 00:00:00