Abstract:
:An LT-B-ST (LT-B/ST) fusion peptide was constructed by genetically joining the 5' terminus of a synthetic gene coding for the heat-stable enterotoxin (ST) of Escherichia coli to the 3' terminus of the gene coding for the binding subunit of the heat-labile enterotoxin (LT-B) of E. coli. An eight-amino-acid, proline-containing linker was included between the LT-B and ST moieties. An aroA mutant of Salmonella dublin transformed with a plasmid carrying this genetic construct was shown to express a fusion peptide with antigenic determinants of both LT-B and ST. Mice were immunized orally with this strain or with a control strain expressing just LT-B from the same plasmid. Sera and mucosal secretions were obtained and analyzed for the presence of serum immunoglobulin G and mucosal immunoglobulin A that were able to recognize LT-B and ST by enzyme-linked immunosorbent assay (ELISA) and, more importantly, were able to neutralize native ST in the suckling mouse assay. Sera and mucosal secretions from animals immunized with the strain expressing the LT-B/ST fusion exhibited detectable ELISA reactivity against LT-B but not against native ST. However, even in the absence of detectable ELISA reactivity, both sera and mucosal secretions from these animals were able to neutralize the biological activity of native ST in the suckling mouse assay. These findings are important because they demonstrate the development of mucosal protection against ST by oral immunization with a genetic fusion delivered by a bacterial vector.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Cárdenas L,Clements JDdoi
10.1128/IAI.61.11.4629-4636.1993subject
Has Abstractpub_date
1993-11-01 00:00:00pages
4629-36issue
11eissn
0019-9567issn
1098-5522journal_volume
61pub_type
杂志文章abstract::We investigated a possible cause-and-effect relationship between sensitization against Actinomyces viscosus Nyl and destructive periodontal disease in RIC-Sprague-Dawley rats. Germfree rats (66) were either immunized with A. viscosus Nyl (day 20) or orally infected with A. viscosus Nyl (days 38 and 39) or both. We mea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.971-977.1981
更新日期:1981-03-01 00:00:00
abstract::Alveolar bone resorption can be induced in specific-pathogen-free mice by oral infection with Porphyromonas gingivalis (P. J. Baker, R. T. Evans, and D. C. Roopenian, Arch. Oral Biol. 39:1035-1040, 1994). Here we used a mouse strain, C57BL/6J, which is relatively resistant to P. gingivalis-induced bone loss to examine...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.6.3103-3107.2000
更新日期:2000-06-01 00:00:00
abstract::Vitamin E protects nonimmunized and immunized mice against fatal Diplococcus pneumoniae type I (DpI) infection. A dietary supplementation of 180 mg of dl-alpha-tocopheryl acetate per kg of diet increased survival of nonimmunized mice from 20 to 80% when challenged with 20 organisms, and of mice immunized with 0.5 ng o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1292-1295.1974
更新日期:1974-12-01 00:00:00
abstract::A gene from Haemophilus influenzae encoding an outer membrane lipoprotein of about 15,000 daltons and which comigrates with the peptidoglycan-associated lipoprotein (PAL) of H. influenzae on sodium dodecyl sulfate-polyacrylamide gel electrophoresis has been previously reported and designated pcp gene, and its product ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.10.3388-3393.1990
更新日期:1990-10-01 00:00:00
abstract::Oral treponemes are considered to be important in the development and progression of periodontal diseases. We investigated the mechanisms of recognition and activation of human gingival epithelial cells (HGEC) with the oral treponemes Treponema denticola, Treponema vincentii, and Treponema medium and their outer membr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.2.717-725.2003
更新日期:2003-02-01 00:00:00
abstract::The dispersed gene family 1 (DGF-1) is the fifth largest gene family in the Trypanosoma cruzi genome, with over 500 members (11). Many of the predicted DGF-1 protein products have several transmembrane domains and N-glycosylation and phosphorylation sites and were thought to localize in the plasma membrane. Here, we r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00780-09
更新日期:2010-01-01 00:00:00
abstract::Streptococcus pneumoniae remains a major causative agent of serious human diseases. The worldwide increase of antibiotic resistant strains revealed the importance of horizontal gene transfer in this pathogen, a scenario that results in the modulation of the species-specific gene pool. We investigated genomic variation...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.4.2477-2486.2001
更新日期:2001-04-01 00:00:00
abstract::The two enteropathogens Yersinia pseudotuberculosis and Yersinia enterocolitica penetrate eukaryotic cells in vitro through invasin, a surface-exposed protein. In contrast, Yersinia pestis, the causative agent of plague, is unable to enter mammalian cell lines, although the inv gene is present on its chromosome. Altho...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.1.375-379.1996
更新日期:1996-01-01 00:00:00
abstract::Neisseria meningitidis is a human-specific pathogen and leading cause of meningitis and septicemia. Factor H binding protein (fHbp), a virulence factor which protects N. meningitidis from innate immunity by binding the human complement regulator factor H (fH) with high affinity, is also a key antigen in vaccines being...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01183-13
更新日期:2014-03-01 00:00:00
abstract::Guinea pig peritoneal or alveolar macrophages were inoculated with Mycoplasma pneumoniae cells. Extracellular mycoplasms were killed by complement treatment, and the effect of macrophage action on the number of the remaining viable mycoplasmas was observed. The complement killing was to some extent inhibited by the pr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.1.11-15.1979
更新日期:1979-07-01 00:00:00
abstract::It has been previously reported that although inducible nitric oxide synthase (iNOS) gene knockout (NOS2(-/-)) mice resolve Chlamydia trachomatis genital infection, the production of reactive nitrogen species (RNS) via iNOS protects a significant proportion of mice from hydrosalpinx formation and infertility. We now r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.12.7374-7379.2001
更新日期:2001-12-01 00:00:00
abstract::Five mutagens were used to induce mutants of Candida albicans. Ultraviolet light, N-nitroso-N'-methyl-N-nitrosoguanidine, and N-nitroso-N-methylurethane were effective mutagens which induced stable auxotrophs. N-nitroso-N-methylurethane produced the largest number and variety of mutants. Nitrous acid and hydroxylamine...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.3.1.141-148.1971
更新日期:1971-01-01 00:00:00
abstract::GNA33 is a membrane-bound lipoprotein with murein hydrolase activity that is present in all Neisseria species and well conserved in different meningococcal isolates. The protein shows 33% identity to a lytic transglycolase (MltA) from Escherichia coli and has been shown to be involved in the degradation of both insolu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.1914-1919.2004
更新日期:2004-04-01 00:00:00
abstract::Specific stimulation of T cells by phytohemagglutinin and Lens culinaris lectin was inhibited by a soluble factor(s) secreted by normal adherent cells stimulated with culture filtrate protein extract (CFPE) derived from bacterial cultures of Mycobacterium tuberculosis H37Ra (avirulent) and H37Rv (virulent). The induct...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.52.1.309-313.1986
更新日期:1986-04-01 00:00:00
abstract::Legionella pneumophila, the gram-negative agent of Legionnaires' disease, possesses type IV pili and a type II protein secretion (Lsp) system, both of which are dependent upon the PilD prepilin peptidase. By analyzing multiple pilD mutants and various types of Lsp mutants as well as performing trans-complementation of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.1.310-321.2004
更新日期:2004-01-01 00:00:00
abstract::Babesiosis has long been recognized as an economically important disease of cattle, but only in the last 30 years has Babesia been recognized as an important pathogen in humans. Invasion of erythrocytes is an integral part of the Babesia life cycle. However, very little information is available on the molecules involv...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.649-651.2005
更新日期:2005-01-01 00:00:00
abstract::A novel in vivo expression technology (IVET) was performed to identify Klebsiella pneumoniae CG43 genes that are specifically expressed during infection of BALB/c mice. The IVET employed a UDP glucose pyrophosphorylase (galU)-deficient mutant of K. pneumoniae which is incapable of utilizing galactose and synthesizing ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.11.7140-7145.2001
更新日期:2001-11-01 00:00:00
abstract::Whole blood and plasma from animals in the acute stage of leptospirosis contained a toxic factor which produced a cytopathic effect on fibroblastic L cell monolayers. Firm adsorption of cytotoxic factor (CTF) to L cells occurred within 1 h. The highest titer of CTF in plasma was reached at 24 h and declined after 48 h...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.3.401-405.1973
更新日期:1973-09-01 00:00:00
abstract::During bacterial infection of the bovine mammary gland, large numbers of leukocytes migrate into the udder, resulting in the establishment of a host response against the pathogen. Currently, the specific leukocyte populations mediating this immune response are not well defined. In the studies described here, we analyz...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::White-footed mice (Peromyscus leucopus), the primary reservoir for Borrelia burgdorferi in the northern midwest and northeastern United States, were experimentally inoculated with an infectious strain or a noninfectious strain of the Lyme disease spirochete and examined for their specific antibody response with the en...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.11.3445-3451.1989
更新日期:1989-11-01 00:00:00
abstract::Trypanosome-activated mouse peritoneal macrophages phagocytized and digested Trypanosoma brucei in vitro and in vivo, but in the absence of specific antiserum and complement the degree of phagocytosis was minimal. Ultrastructurally, the parasites attached to the macrophage by their flagella, and ingestion proceeded fl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.19.3.972-982.1978
更新日期:1978-03-01 00:00:00
abstract::An apparent inhibition of induction of mouse hepatic tryptophan oxygenase by endotoxin has been reported previously, as evidenced by low catalytic activity. This could be due either to decreased tryptophan oxygenase levels or to inactivation of existing enzyme molecules. To resolve this question, the enzyme was quanti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.4.534-539.1973
更新日期:1973-10-01 00:00:00
abstract::Neutrophils are the main inflammatory cell present in lesions involving the central nervous system (CNS) during human and murine listeriosis. In this study, administration of the neutrophil-depleting monoclonal antibody RB6-8C5 during experimental murine listeriosis facilitated the multiplication of Listeria monocytog...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.8.4789-4791.2000
更新日期:2000-08-01 00:00:00
abstract::Porphyromonas gingivalis has been implicated as an etiologic agent of adult periodontitis. We have previously shown that P. gingivalis can degrade the epithelial cell-cell junction complexes, thus suggesting that this bacterium can invade the underlying connective tissues via a paracellular pathway. However, the preci...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.5.2512-2518.2002
更新日期:2002-05-01 00:00:00
abstract::Normal ICR mice were infected intravenously, intraperitoneally, or aerogenically with Pasteurella multocida strains isolated from a turkey (S68), calf (V90), or rabbit (J20) lung. Both the turkey and calf isolates were highly virulent for mice and multiplied logarithmically in the lungs, liver, and spleen, resulting i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.779-784.1983
更新日期:1983-02-01 00:00:00
abstract::The adjuvant action of various lipopolysaccharides on immune responses to syngeneic tissue extract in mice was examined. Only lipopolysaccharides possessing the linear mannose homopolysaccharides as O-specific polysaccharides exhibited definite adjuvant action on immune responses to the autoantigens. The intensity of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4953-4956.1992
更新日期:1992-11-01 00:00:00
abstract::The filarial nematode Onchocerca volvulus is the causative organism of river blindness. Our previous studies demonstrated an essential role for endosymbiotic Wolbachia bacteria in corneal disease, which is characterized by neutrophil infiltration into the corneal stroma and the development of corneal haze. To determin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00991-07
更新日期:2007-12-01 00:00:00
abstract::The cell surface hydrophobicity of 10 pigmented and 4 nonpigmented clinical Serratia marcescens strains was studied, based on the ability of the strains to adhere to hydrocarbons and to polystyrene. The cell surface hydrophobicity depended greatly on growth temperature; all of the strains tested were adherent followin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.3.932-935.1986
更新日期:1986-03-01 00:00:00
abstract::Streptococcal group A, B, and C carbohydrates were analyzed by counterimmunoelectrophoresis, immunoelectrophoresis, and inhibition of immunoprecipitation. Extracts of streptococci group A or C were shown by counterimmunoelectrophoresis to contain both anodic and cathodic migrating components. In immunoelectrophoresis,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.209-215.1982
更新日期:1982-07-01 00:00:00
abstract::The virulence of Candida albicans in a mouse model of invasive candidiasis is dependent on the phospholipids phosphatidylserine (PS) and phosphatidylethanolamine (PE). Disruption of the PS synthase gene CHO1 (i.e., cho1Δ/Δ) eliminates PS and blocks the de novo pathway for PE biosynthesis. In addition, the cho1Δ/Δ muta...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01612-14
更新日期:2014-10-01 00:00:00