Abstract:
:Moraxella catarrhalis expresses surface receptor proteins that specifically bind host transferrin (Tf) and lactoferrin (Lf) in the first step of the iron acquisition pathway. Acute- and convalescent-phase antisera from a series of patients with M. catarrhalis pulmonary infections were tested against Tf and Lf receptor proteins purified from the corresponding isolates. After the purified proteins had been separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blotting, we observed strong reactivity against Tf-binding protein B (TbpB; also called OMP1) and Lf-binding protein B (LbpB) but little or no reactivity against Tf-binding protein A (TbpA) or Lf-binding protein A (LbpA), using the convalescent-phase antisera. Considerable antigenic heterogeneity was observed when TbpBs and LbpBs isolated from different strains were tested with the convalescent-phase antisera. Comparison to the reactivity against electroblotted total cellular proteins revealed that the immune response against LbpB and TbpB constitutes a significant portion of the total detectable immune response to M. catarrhalis proteins. Preparations of affinity-isolated TbpA and LbpA reacted with convalescent-phase antisera in a solid-phase binding assay, but blocking with soluble TbpB, soluble LbpB, or extracts from an LbpA(-) mutant demonstrated that this reactivity was attributed to contaminants in the TbpA and LbpA preparations. These studies demonstrate the immunogenicity of M. catarrhalis TbpB and LbpB in humans and support their potential as vaccine candidates.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Yu RH,Bonnah RA,Ainsworth S,Schryvers ABdoi
10.1128/IAI.67.8.3793-3799.1999keywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
3793-9issue
8eissn
0019-9567issn
1098-5522journal_volume
67pub_type
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更新日期:1983-07-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.6.2200-2202.1991
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.5.1127-1134.1974
更新日期:1974-11-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.8.3611-3617.1998
更新日期:1998-08-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章,多中心研究
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更新日期:1998-01-01 00:00:00
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journal_title:Infection and immunity
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更新日期:2004-09-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:2003-05-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1984-06-01 00:00:00