Cytotoxicity of Mycoplasma pneumoniae Membranes.

abstract：:Plasmodium ookinetes secrete chitinases to penetrate the acellular, chitin-containing peritrophic matrix of the mosquito midgut en route to invasion of the epithelium. Chitinases are potentially targets that can be used to block malaria transmission. We demonstrate here that chitinases of Plasmodium falciparum and P. ...

journal_title：Infection and immunity

authors： Langer RC,Hayward RE,Tsuboi T,Tachibana M,Torii M,Vinetz JM

更新日期：2000-11-01 00:00:00

abstract：:The amount of crude Campylobacter jejuni enterotoxin present in culture products was quantitated by comparing the response of these preparations with that of pure Escherichia coli heat-labile toxin (LT) in the Chinese hamster ovary assay and in enzyme-linked immunosorbent assays that used GM ganglioside or antisera to...

journal_title：Infection and immunity

authors： Klipstein FA,Engert RF

更新日期：1984-08-01 00:00:00

abstract：:A live, attenuated, recombinant influenza vaccine (Alice strain) administered intranasally was evaluated in high school students and compared with intranasal placebo and subcutaneous, inactivated, bivalent influenza vaccine. The Alice strain was antigenic, increasing the geometric mean titer (GMT) from a prestudy leve...

journal_title：Infection and immunity

authors： Schiff GM,Linnemann CC Jr,shea L,Lange B,Rotte T

更新日期：1975-04-01 00:00:00

abstract：:Nitration of protein A with tetranitromethane incompletely blocks its Fc-binding ability for immunoglobulin G when measured by more sensitive methods of immunoprecipitation and when tested in the enzyme-linked immunosorbent assay. In addition, binding of protein A and blockage by nitration is heterogeneous and depends...

journal_title：Infection and immunity

authors： Sloan GJ,Butler JE

更新日期：1978-08-01 00:00:00

abstract：:Sepsis is both a common and, despite present-day therapy, a serious disease. The pathophysiology of the septic response is a complex, multifactorial phenomenon which in part involves the activation of complement by bacterial endotoxin. A monoclonal antibody to human complement factor B, code-named 1H5, which was capab...

journal_title：Infection and immunity

authors： Clardy CW

更新日期：1994-10-01 00:00:00

abstract：:Treponema hyodysenteriae was shown to attach to mouse peritoneal cells in the absence of serum opsonins in vitro. If serotype-specific antiserum from pigs was added to the media and treponemes of that corresponding serotype were employed in the assay, the amount of attachment increased an average of 3.7 times that of ...

journal_title：Infection and immunity

authors： Nuessen ME,Joens LA

更新日期：1982-12-01 00:00:00

abstract：:Some clinical strains of Vibrio cholerae non-O1 produce an extracellular factor that evokes a rapid and dramatic cytotoxic response which manifests as cell rounding of Chinese hamster ovary (CHO) and HeLa cells without accompanying membrane damage. This study was performed to establish the identity of the non-membrane...

journal_title：Infection and immunity

authors： Saha PK,Koley H,Nair GB

更新日期：1996-08-01 00:00:00

abstract：:EspC is an autotransporter protein secreted by enteropathogenic Escherichia coli (EPEC). The pathogenic role of EspC in EPEC infection is unknown. We have shown that the purified EspC produces enterotoxicity and cytotoxicity; for the latter effect, EspC must be internalized. However, the internalization mechanism is u...

journal_title：Infection and immunity

authors： Vidal JE,Navarro-García F

更新日期：2006-04-01 00:00:00

abstract：:We identified, by two-dimensional electrophoretic analysis and microsequencing, a protein of Chlamydia trachomatis elementary bodies which corresponds to the polypeptide (pgp3) encoded by open reading frame 3 (ORF3). Amino acid analysis showed that the first residue (Gly) found in the native protein is the one encoded...

journal_title：Infection and immunity

authors： Comanducci M,Manetti R,Bini L,Santucci A,Pallini V,Cevenini R,Sueur JM,Orfila J,Ratti G

更新日期：1994-12-01 00:00:00

abstract：:Infections caused by organisms of the Mycobacterium avium complex occur in approximately 50 to 60% of patients with AIDS. M. avium is an intracellular pathogen that survives and multiplies within mononuclear phagocytes. In this study, we investigated the uptake of M. avium grown within macrophages (intracellular growt...

journal_title：Infection and immunity

authors： Bermudez LE,Parker A,Goodman JR

更新日期：1997-05-01 00:00:00

abstract：:Human peripheral blood monocytes become apoptotic following phagocytosis of Staphylococcus aureus. In this study, we investigated the mechanisms involved in this phenomenon. Cells exposed to bacteria were examined for the surface expression of Fas and Fas ligand (FasL). The level of soluble form of FasL was also measu...

journal_title：Infection and immunity

authors： Baran J,Weglarczyk K,Mysiak M,Guzik K,Ernst M,Flad HD,Pryjma J

更新日期：2001-03-01 00:00:00

abstract：:Type II heat-labile enterotoxin (LT-II) from Escherichia coli 41 was purified and compared with prototype LT-II encoded by genes from E. coli SA53. Both toxins were oligomeric proteins consisting of polypeptides A (Mr, 28,000) and B (Mr, 11,800). The A polypeptides were cleaved by trypsin into fragments A1 (Mr, 21,000...

journal_title：Infection and immunity

authors： Guth BE,Twiddy EM,Trabulsi LR,Holmes RK

更新日期：1986-11-01 00:00:00

abstract：:Staphylococcus aureus biofilm formation is induced in iron-restricted growth conditions in vitro. In this study, we showed that Emp and Eap play important roles in low-iron-induced biofilm formation of S. aureus Newman. Eap and Emp are secreted proteins which are non-covalently attached to the S. aureus cell surface a...

journal_title：Infection and immunity

authors： Johnson M,Cockayne A,Morrissey JA

更新日期：2008-04-01 00:00:00

abstract：:We have studied the ability of outer membrane vesicle (OMV) vaccines from Neisseria meningitidis serogroup B to induce vaccine-specific antibody and spleen cell proliferative responses in mice after being administered intranasally (i.n.) and/or subcutaneously (s.c.). A series of four weekly i.n. doses (25 microg) with...

journal_title：Infection and immunity

authors： Bakke H,Lie K,Haugen IL,Korsvold GE,Høiby EA,Naess LM,Holst J,Aaberge IS,Oftung F,Haneberg B

更新日期：2001-08-01 00:00:00

abstract：:A Mycoplasma pneumoniae cytadhesin P1 gene with novel nucleotide sequence variation has been identified. Four clinical strains of M. pneumoniae were found to carry this type of P1 gene. This new P1 gene is similar to the known group II P1 genes but possesses novel sequence variation of approximately 300 bp in the RepM...

journal_title：Infection and immunity

authors： Kenri T,Taniguchi R,Sasaki Y,Okazaki N,Narita M,Izumikawa K,Umetsu M,Sasaki T

更新日期：1999-09-01 00:00:00

abstract：:The antigenically conserved proteins of Treponema pallidum subsp. pallidum and four nonpathogenic cultivatable treponemes were investigated by phase partitioning with the nonionic detergent Triton X-114 and immunoblot analysis. None of the T. pallidum integral membrane proteins identified by phase partitioning (deterg...

journal_title：Infection and immunity

authors： Radolf JD,Norgard MV

更新日期：1988-07-01 00:00:00

abstract：:Leukosis viruses of seven subgroups were tested for oncogenicity in chickens susceptible to virus infection and to development of lymphoid leukosis (LL) tumors. All subgroup A viruses and the subgroup B virus tested produced a high incidence of LL and other related neoplasms. Viruses of subgroup C and RAV-61 of subgro...

journal_title：Infection and immunity

authors： Purchase HG,Okazaki W,Vogt PK,Hanafusa H,Burmester BR,Crittenden LB

更新日期：1977-02-01 00:00:00

abstract：:Protective immunity against Babesia bigemina is hypothesized to involve antibodies directed against merozoite surface-exposed epitopes. Levels of antibody against a rhoptry-associated protein 1 (RAP-1) B-lymphocyte epitope, defined by surface-reactive and inhibitory monoclonal antibodies, in immune cattle sera were de...

journal_title：Infection and immunity

authors： Ushe TC,Palmer GH,Sotomayor L,Figueroa JV,Buening GM,Perryman LE,McElwain TF

更新日期：1994-12-01 00:00:00

abstract：:Chemokines (CK) are potent leukocyte activators and chemoattractants and aid in granuloma formation, functions critical for the immune response to Mycobacterium tuberculosis. We hypothesized that infection of alveolar macrophages (AM) with different strains of M. tuberculosis elicits distinct profiles of CK, which cou...

journal_title：Infection and immunity

authors： Saukkonen JJ,Bazydlo B,Thomas M,Strieter RM,Keane J,Kornfeld H

更新日期：2002-04-01 00:00:00

abstract：:A clone expressing a 35.5-kDa recombinant treponemal protein was isolated from a genomic DNA library constructed from Treponema pallidum street strain 14. Polyclonal antiserum raised against the recombinant protein reacted with a corresponding native protein of comparable size in T. pallidum that is specific to the pa...

journal_title：Infection and immunity

authors： Hubbard CL,Gherardini FC,Bassford PJ Jr,Stamm LV

更新日期：1991-04-01 00:00:00

abstract：:Human defensins play a fundamental role in the initiation of innate immune responses to some microbial pathogens. Here we show that colonic epithelial model HCT116 cells respond to Trypanosoma cruzi infection by secreting defensin α-1, which reduces infection. We also report the early effects of defensin α-1 on invasi...

journal_title：Infection and immunity

authors： Johnson CA,Rachakonda G,Kleshchenko YY,Nde PN,Madison MN,Pratap S,Cardenas TC,Taylor C,Lima MF,Villalta F

更新日期：2013-11-01 00:00:00

abstract：:Cell-mediated immune (CMI) responses and tumor necrosis factor alpha (TNF-alpha) have been shown to be essential in acquired protection against Cryptococcus neoformans. Induction of a protective anticryptococcal CMI response includes increases in dendritic cells (DC) and activated CD4(+) T cells in draining lymph node...

journal_title：Infection and immunity

authors： Bauman SK,Huffnagle GB,Murphy JW

更新日期：2003-01-01 00:00:00

abstract：:The adenylate cyclase-hemolysin of Bordetella pertussis consists of a cell-invasive N-terminal adenylate cyclase domain linked to a C-terminal RTX hemolysin containing extensive glycine-rich repeats. The toxin is an essential virulence factor required in the initial stages of infection. Adenylate cyclase-hemolysin was...

journal_title：Infection and immunity

authors： Betsou F,Sebo P,Guiso N

更新日期：1995-09-01 00:00:00

abstract：:Enteropathogenic strains of Yersinia enterocolitica harbor a virulence plasmid (70 kilobases) which specifies, at 37 degrees C, a calcium requirement for growth, autoagglutinability, resistance to the bactericidal activity of human serum, and the expression of some outer membrane proteins (OMPs). To map the genes enco...

journal_title：Infection and immunity

authors： Balligand G,Laroche Y,Cornelis G

更新日期：1985-06-01 00:00:00

abstract：:Acute pyelonephritis, a complication of Escherichia coli bacteriuria, must represent a bacterial invasion through the kidney epithelium. To study this process, we overlaid bacterial suspensions onto monolayers of cultured human kidney proximal tubular epithelial cells and measured cytotoxicity by release of lactate de...

journal_title：Infection and immunity

authors： Mobley HL,Green DM,Trifillis AL,Johnson DE,Chippendale GR,Lockatell CV,Jones BD,Warren JW

更新日期：1990-05-01 00:00:00

abstract：:Cord factor (trehalose 6,6'-dimycolate[TDM] ), a mixture of 6,6'-diesters of alpha, alpha-D-trehalose with natural mycolic acids, has been described as having immunoregulatory and antitumor activities in vivo, although the relevant mechanisms of action remain unelucidated. In this work, we measured the effects of TDM ...

journal_title：Infection and immunity

authors： Kierszenbaum F,Zenian A,Wirth JJ

更新日期：1984-02-01 00:00:00

abstract：:Toxoplasma gondii is an obligate intracellular parasite that resides in the cytoplasm of its host in a unique membrane-bound vacuole known as the parasitophorous vacuole (PV). The membrane surrounding the parasite is remodeled by the dense granules, secretory organelles that release an array of proteins into the vacuo...

journal_title：Infection and immunity

authors： Rome ME,Beck JR,Turetzky JM,Webster P,Bradley PJ

更新日期：2008-11-01 00:00:00

abstract：:The multiple antigen peptide (MAP) approach is an effective method to chemically synthesize and deliver multiple T-cell and B-cell epitopes as the constituents of a single immunogen. Here we report on the design, chemical synthesis, and immunogenicity of three Plasmodium falciparum MAP vaccines that incorporated antig...

journal_title：Infection and immunity

authors： Mahajan B,Berzofsky JA,Boykins RA,Majam V,Zheng H,Chattopadhyay R,de la Vega P,Moch JK,Haynes JD,Belyakov IM,Nakhasi HL,Kumar S

更新日期：2010-11-01 00:00:00

abstract：:Ocular exposure of mice to live elementary bodies of Chlamydia trachomatis serovar A results in immunological sensitization of the mice. This reactivity is manifested by the development of early (5 h) and delayed-type (24 h) dermal reactivity and serovar-specific antibody formation against either live or irradiated (1...

journal_title：Infection and immunity

authors： Colley DG,Goodman TG,Barsoum IS

更新日期：1986-10-01 00:00:00

abstract：:We have discovered two distinctly different fimbriae expressed by the same Porphyromonas gingivalis strain. The construction of a fimA mutant of P. gingivalis ATCC 33277 has previously been reported by N. Hamada et al. (Infect. Immun. 62:1696-1704, 1994). Expression of fimbriae on the surface of the fimA mutant and th...

journal_title：Infection and immunity

authors： Hamada N,Sojar HT,Cho MI,Genco RJ

更新日期：1996-11-01 00:00:00