Abstract:
:The relationship between the sequence of Neisseria gonorrhoeae pilin and its quaternary assembly into pilus fibers was studied with a set of site-directed antibody probes and by mapping the specificities of antipilus antisera with peptides. Buried and exposed peptides in assembled pili were identified by competitive immunoassays and immunoelectron microscopy with polyclonal antibodies raised against 11 peptides spanning the pilin sequence. Pili did not compete significantly with pilin subunits for binding to antibodies against residues 13 to 31 (13-31) and 18-36. Pilus fibers competed well with pilin protein subunits for binding to antibodies raised against peptides 37-56, 58-78, 110-120, 115-127, 122-139, and 140-159 and competed weakly for antibodies against residues 79-93 and 94-108. Antibodies to sequence-conserved residues 37-56 and to semiconserved residues 94-108 preferentially bound pilus ends as shown by immunoelectron microscopy. The exposure of pilus regions to the immune system was tested by peptide mapping of antiserum specificities against sets of overlapping peptides representing all possible hexameric or octameric peptides from the N. gonorrhoeae MS11 pilin sequence. The immunogenicity of exposed peptides incorporating semiconserved residues 49-56 and 121-126 was revealed by strong, consistent antigenic reactivity to these regions measured in antipilus sera from rabbits, mice, and human and in sera from human volunteers with gonorrhea. The conservation and variation of antigenic responses among these three species clarify the relevance of immunological studies of other species to the human immune response against pathogens. Overall, our results explain the extreme conservation of the entire N-terminal one-third of the pilin protein by its dominant role in pilus assembly: hydrophobic residues 1-36 are implicated in buried lateral contacts, and polar residues 37-56 are implicated in longitudinal contacts within the pilus fiber.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Forest KT,Bernstein SL,Getzoff ED,So M,Tribbick G,Geysen HM,Deal CD,Tainer JAdoi
10.1128/IAI.64.2.644-652.1996subject
Has Abstractpub_date
1996-02-01 00:00:00pages
644-52issue
2eissn
0019-9567issn
1098-5522journal_volume
64pub_type
杂志文章abstract::The predominant gram-positive bacteria in 47 fecal specimens from 10 healthy men were studied by microscopic and cultural counts, by the characterization and tentative identification of isolates, and by the use of fluorescein isothiocyanate (FITC)-conjugated globulins prepared using some of the isolates. Gram-positive...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.4.719-729.1974
更新日期:1974-04-01 00:00:00
abstract::To explore the mechanisms underlying the eosinophil-mediated inflammation of tropical pulmonary eosinophilia (TPE), bronchoalveolar lavage (BAL) fluid, serum, and supernatants from pulmonary and blood leukocytes (WBC) from patients with acute TPE (n = 6) were compared with those obtained from healthy uninfected indivi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.3.1337-1342.2003
更新日期:2003-03-01 00:00:00
abstract::The abilities of six strains of Mycobacterium bovis strain BCG to replicate in vivo and to cause spleen enlargement in the CDF(1) male mouse were compared. The strains were grown in synthetic medium and harvested soon after complete pellicles were produced on liquid medium. In addition, two freeze-dried commercially a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.8.5.736-742.1973
更新日期:1973-11-01 00:00:00
abstract::The Toll-like receptor 2 (TLR2)/TLR1 receptor complex responds to amyloid fibrils, a common component of biofilm material produced by members of the phyla Firmicutes, Bacteroidetes, and Proteobacteria. To determine whether this TLR2/TLR1 ligand stimulates inflammatory responses when bacteria enter intestinal tissue, w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00911-12
更新日期:2012-12-01 00:00:00
abstract::The cell surface hydrophobicity of 10 pigmented and 4 nonpigmented clinical Serratia marcescens strains was studied, based on the ability of the strains to adhere to hydrocarbons and to polystyrene. The cell surface hydrophobicity depended greatly on growth temperature; all of the strains tested were adherent followin...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.3.932-935.1986
更新日期:1986-03-01 00:00:00
abstract::To assess the relationship between the within-host diversity of malaria infections and the susceptibility of the host to subsequent infection, we genotyped 60 children's successive infections from birth through 3 years of life. MSP-1 Block2 genotypes were used to estimate the complexity of infection (COI). Malaria tra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.12.7783-7792.2001
更新日期:2001-12-01 00:00:00
abstract::Lipooligosaccharide (LOS) is a major surface component of Moraxella catarrhalis and a possible virulence factor in the pathogenesis of human infections caused by this organism. The presence of LOS on the bacterium is an obstacle to the development of vaccines derived from whole cells or outer membrane components of th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.11.7569-7577.2005
更新日期:2005-11-01 00:00:00
abstract::Vesicular stomatitis virus (VSV) infection of beige (bg/bg) mice induced levels of natural killer cytolytic activity comparable to that of uninfected normal bg/+ controls, but considerably less than natural killer activity in VSV-infected bg/+ mice. In contrast, VSV-infected bg/bg and bg/+ mice had essentially equival...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.1.204-210.1981
更新日期:1981-04-01 00:00:00
abstract::The development and course of normally lethal parasitemias in mice inoculated intraperitoneally with erythrocytic stages of Plasmodium yoelii or Plasmodium berghei were markedly affected by treatment with the antilymphoid drug cyclosporin A (CS-A). When the first of four daily subcutaneous 25-mg/kg doses of CS-A was g...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.3.1093-1100.1982
更新日期:1982-09-01 00:00:00
abstract::Helicobacter pylori maintains colonization in its human host using a limited set of taxis sensors. TlpD is a proposed energy taxis sensor of H. pylori and dominant under environmental conditions of low bacterial energy yield. We studied the impact of H. pylori TlpD on colonization in vivo using a gerbil infection mode...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00750-13
更新日期:2013-10-01 00:00:00
abstract::Many of the virulence factors associated with fulminant group A streptococci (GAS) infection are expressed under in vitro exponential growth conditions. However, the survival of GAS in tissue and intracellularly, as well as colonization of asymptomatic carriers, has been reported for GAS. The bacteria associated with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.8.3841-3847.1998
更新日期:1998-08-01 00:00:00
abstract::Antigens shared between Streptococcus pyogenes and heart tissue may play an important role in autoimmune cardiac injury associated with acute rheumatic fever. Antiheart/antistreptococcal antibodies found in the disease react with antigens of S. pyogenes, including M protein and a 60-kDa antigen distinct from M protein...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.9.3932-3936.1992
更新日期:1992-09-01 00:00:00
abstract::Studies of mice suggest that pneumococcal proteins, including PspA, pneumolysin, PsaA, and CbpA, are promising vaccine candidates. To determine whether these proteins are good mucosal immunogens in humans, adenoidal lymphocytes from 20 children who had adenoidectomies were isolated and tested by ELISpot for antigen-sp...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.10.5363-5369.2002
更新日期:2002-10-01 00:00:00
abstract::Candida albicans secreted aspartyl proteinases (Saps) are considered virulence-associated factors. Several members of the Sap family were claimed to play a significant role in the progression of candidiasis established by the hematogenous route. This assumption was based on the observed attenuated virulence of sap-nul...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00248-10
更新日期:2010-11-01 00:00:00
abstract::A C3d-binding glycoprotein was purified from the culture filtrate of Candida albicans by preparative isoelectric focusing. The protein possessed a pI of 3.9 to 4.1 and could inhibit rosetting of EAC3d (sheep erythrocytes conjugated to C3d) by pseudohyphae of C. albicans. When analyzed by polyacrylamide gel electrophor...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.2.309-314.1990
更新日期:1990-02-01 00:00:00
abstract::Lipopolysaccharide (LPS) can induce mouse macrophages to produce a number of cytokines and other inflammatory mediators. Our laboratory previously reported that LPS-dependent macrophage-derived tumor necrosis factor alpha (TNF-alpha) production could be significantly potentiated by pretreatment with LPS at substimulat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.3.1011-1015.1996
更新日期:1996-03-01 00:00:00
abstract::Association of Salmonella typhimurium with MDCK epithelial cells in monolayers, represented primarily by intracellular bacteria after 30 min of contact, with centrifugation followed by vigorous washing, was measured during aerobic and anaerobic growth of the bacteria in brain heart infusion broth. Cell association was...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1462-1467.1995
更新日期:1995-04-01 00:00:00
abstract::Nonimmune binding of immunoglobulin to whole bacteria was quantitated for North American isolates of Haemophilus somnus recovered from cattle with pneumonia, reproductive failure (abortion), or thromboembolic meningoencephalitis or from the vagina or prepuce of carrier cattle. Quantitative binding activity covered a w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.2.639-642.1989
更新日期:1989-02-01 00:00:00
abstract::Knowledge of the in vivo physiology and metabolism of Streptococcus pneumoniae is limited, even though pneumococci rely on efficient acquisition and metabolism of the host nutrients for growth and survival. Because the nutrient-limited, hypoxic host tissues favor mixed-acid fermentation, we studied the role of the pne...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00178-09
更新日期:2009-12-01 00:00:00
abstract::ExoU, a phospholipase injected into host cells by the type III secretion system of Pseudomonas aeruginosa, leads to rapid cytolytic cell death. Although the importance of ExoU in infection is well established, the mechanism by which this toxin kills host cells is less clear. To gain insight into how ExoU causes cell d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.5.2552-2561.2006
更新日期:2006-05-01 00:00:00
abstract::Herein we characterized various genetic markers and the biological behavior of a natural recombinant strain of Toxoplasma gondii (P-Br). From nine genetic markers analyzed, three (B1, ROP1, and SAG1) and three (cS10-A6, GRA6, and SAG3) markers belong to parasites from the type I and type III lineages, respectively. Th...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.11.6392-6401.2003
更新日期:2003-11-01 00:00:00
abstract::Heat-labile enterotoxins from Escherichia coli strains of porcine and human origin polymerize on heating to form high-molecular-weight aggregates, "procoligenoids," analogous to procholeragenoid derived from the cholera enterotoxin. This aggregation is accompanied by loss of biological activity (toxicity). Further hea...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.2.518-521.1984
更新日期:1984-08-01 00:00:00
abstract::Enterococcus faecalis strains are resident intestinal bacteria associated with invasive infections, inflammatory bowel diseases, and colon cancer. Although factors promoting E. faecalis colonization of intestines are not fully known, one implicated pathway is a phosphotransferase system (PTS) in E. faecalis strain OG1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00080-19
更新日期:2019-06-20 00:00:00
abstract::We have studied the ability of outer membrane vesicle (OMV) vaccines from Neisseria meningitidis serogroup B to induce vaccine-specific antibody and spleen cell proliferative responses in mice after being administered intranasally (i.n.) and/or subcutaneously (s.c.). A series of four weekly i.n. doses (25 microg) with...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.8.5010-5015.2001
更新日期:2001-08-01 00:00:00
abstract::Borrelia burgdorferi, the causative agent of Lyme disease, loses its ability to infect and cause disease in mammalian hosts after repeated in vitro passage. To identify proteins preferentially expressed by the low-passage strain and thus representing potential virulence factors, the polypeptide profiles of virulent, l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.11.4662-4672.1992
更新日期:1992-11-01 00:00:00
abstract::The effects of an ornithine-containing lipid [alpha-N-(3-acyloxyacyl)-ornithine (Orn-L)] or a serine-containing lipid [alpha-N-(3-acyloxyacyl)-serine (Ser-L)] from Flavobacterium meningosepticum on lethal endotoxemia in mice were examined. When 500 micrograms of Orn-L was intravenously administered 1 h before intraven...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2560-2566.1991
更新日期:1991-08-01 00:00:00
abstract::Shigella flexneri 2a is an invasive enteric pathogen that may produce diarrhea when ingested by human beings and subhuman primates. We have previously shown that shigella diarrhea correlates with water and electrolyte transport abnormalities in the jejunum and colon. Dysentery alone is associated only with colonic tra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.2.368-371.1976
更新日期:1976-08-01 00:00:00
abstract::We have isolated plasma cell hybridomas which secrete monoclonal antibodies directed against Haemophilus ducreyi. Two of these monoclonal antibodies recognize all strains of H. ducreyi tested to date and are capable of detecting the presence of H. ducreyi in skin lesions produced by this pathogen in experimental anima...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.44.1.196-198.1984
更新日期:1984-04-01 00:00:00
abstract::Although it has been known for some time that Salmonella typhimurium is able to survive and even replicate in the normally bactericidal environment of the macrophage phagosome, the mechanisms by which this organism accomplishes this feat remain obscure. In this study, a murine macrophage cell line and confocal immunof...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.4.1475-1485.1997
更新日期:1997-04-01 00:00:00
abstract::V beta 10(+) and V beta 14(+) T cells were selectively increased 7 to 14 days following infection in the lungs of naive mice infected with Histoplasma capsulatum. Following secondary challenge of immune mice, V beta 1(+) and V beta 8.1(+) cells were sporadically increased. Elimination of V beta 10(+) and V beta 14(+) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.3.1648-1652.2002
更新日期:2002-03-01 00:00:00