Abstract:
:Lipooligosaccharide (LOS) is a major surface component of Moraxella catarrhalis and a possible virulence factor in the pathogenesis of human infections caused by this organism. The presence of LOS on the bacterium is an obstacle to the development of vaccines derived from whole cells or outer membrane components of the bacterium. An lpxA gene encoding UDP-N-acetylglucosamine acyltransferase responsible for the first step of lipid A biosynthesis was identified by the construction and characterization of an isogenic M. catarrhalis lpxA mutant in strain O35E. The resulting mutant was viable despite the complete loss of LOS. The mutant strain showed significantly decreased toxicity by the Limulus amebocyte lysate assay, reduced resistance to normal human serum, reduced adherence to human epithelial cells, and enhanced clearance in lungs and nasopharynx in a mouse aerosol challenge model. Importantly, the mutant elicited high levels of antibodies with bactericidal activity and provided protection against a challenge with the wild-type strain. These data suggest that the null LOS mutant is attenuated and may be a potential vaccine candidate against M. catarrhalis.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Peng D,Hong W,Choudhury BP,Carlson RW,Gu XXdoi
10.1128/IAI.73.11.7569-7577.2005keywords:
subject
Has Abstractpub_date
2005-11-01 00:00:00pages
7569-77issue
11eissn
0019-9567issn
1098-5522pii
73/11/7569journal_volume
73pub_type
杂志文章abstract::Brucella organisms are intracellular stealth pathogens of animals and humans. The bacteria overcome the assault of innate immunity at early stages of an infection. Removal of polymorphonuclear neutrophils (PMNs) at the onset of adaptive immunity against Brucella abortus favored bacterial elimination in mice. This was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00118-19
更新日期:2019-04-23 00:00:00
abstract::The 120-kDa rickettsial outer membrane protein (rOmpB) is encoded by a gene with the capacity to encode a protein of approximately 168 kDa. The carboxy-terminal end of the molecule is apparently cleaved to yield 120- and 32-kDa products. Both polypeptides are surface exposed and remain associated with the outer membra...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.1.159-165.1992
更新日期:1992-01-01 00:00:00
abstract::Erysipelothrix rhusiopathiae is a primary pathogen of swine and turkeys and sporadic cause of disease in a variety of other hosts, including humans. A genomic library of the highly virulent strain of E. rhusiopathiae E1-6P was constructed in the expression-cloning vector lambda gt11 and screened with serum from a pig ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.9.3116-3121.1990
更新日期:1990-09-01 00:00:00
abstract::Type III and type VI secretion systems (T3SSs and T6SSs, respectively) are critical virulence determinants in several Gram-negative pathogens. In Burkholderia pseudomallei, the T3SS-3 and T6SS-1 clusters have been implicated in bacterial virulence in mammalian hosts. We recently discovered a regulatory cascade that co...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05148-11
更新日期:2011-08-01 00:00:00
abstract::Wild-type Vibrio cholerae of both El Tor and classical biotypes (strains N16961 and 395, respectively) and nonmotile mutant derivatives with and without flagellar structures were characterized in three different animal models: (i) the rabbit ileal loop, (ii) the removable intestinal tie adult rabbit diarrhea (RITARD) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.8.2727-2736.1991
更新日期:1991-08-01 00:00:00
abstract::LipL32 is the major outer membrane protein in pathogenic Leptospira. It is highly conserved throughout pathogenic species and is expressed in vivo during human infection. While these data suggest a role in pathogenesis, a function for LipL32 has not been defined. Outer membrane proteins of gram-negative bacteria are t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01643-07
更新日期:2008-05-01 00:00:00
abstract::Cells of the laboratory strain 3153A of Candida albicans can be stimulated to undergo high-frequency phenotypic switching by a low dose of UV. We have compared the adhesive properties of cells exhibiting the basic original smooth (o-smooth) phenotype and three switch phenotypes (star, irregular wrinkle, and revertant ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.4.1328-1335.1994
更新日期:1994-04-01 00:00:00
abstract::Knowledge of whether Mycobacterium tuberculosis resides within a relatively impermeable membrane-bound vacuole or is free within the cytoplasm within its host cell is central to an understanding of the immunobiology of this intracellular parasite but is a matter of controversy. To explore this issue, we assessed the a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.10.5800-5807.2002
更新日期:2002-10-01 00:00:00
abstract::Individuals in areas of Plasmodium falciparum endemicity develop immunity to malaria after repeated exposure. Knowledge of the acquisition and nature of protective immune responses to P. falciparum is presently limited, particularly for young children. We examined antibodies (IgM, IgG, and IgG subclasses) to merozoite...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02398-14
更新日期:2015-02-01 00:00:00
abstract::The quantity of membrane-bound and extracellular exotoxin A in four strains of Pseudomonas aeruginosa was investigated. In strain PA-103, which is the prototype strain used for toxin production, all of the toxin was released into the growth medium and little toxin remained with the cell envelope. In a virulent strain ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-05-01 00:00:00
abstract::An attenuated influenza A candidate vaccine virus, derived from the A/Ann Arbor/6/60 (H2N2) cold-adapted (ca) donor virus and the A/Alaska/6/77 (H3N2) wild-type virus, was evaluated in adult seronegative volunteers (serum hemagglutination-inhibiting antibody titer, less than or equal to 1:8) for level of attenuation, ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.3.1044-1051.1983
更新日期:1983-06-01 00:00:00
abstract::For the first time, hepatitis A viral antigen (HAAg) was shown in liver biopsy tissue from a patient in the acute phase of hepatitis type A by light and electron microscopy, using the peroxidase-antibody technique. Under light microscopy, the staining for HAAg appeared as a fine, granular reaction product, scattered t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.1.320-324.1982
更新日期:1982-04-01 00:00:00
abstract::The antigenically variant Chlamydia trachomatis major outer membrane protein (MOMP) is a target of antibody-mediated neutralization in vitro, and it is an important protein for designing a subunit vaccine. Knowledge of MOMP T-cell determinants will be essential to elicit rapid and strong immune responses following an ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.9.3714-3718.1992
更新日期:1992-09-01 00:00:00
abstract::Piglets suckled by dams that had been vaccinated with K88 antigen were significantly more resistant to deaths caused by neonatal diarrhea after challenge with a large dose of a K88-positive enteropathogenic strain of Escherichia coli than piglets suckled by control dams. The factors most likely to be involved in prote...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.13.3.667-676.1976
更新日期:1976-03-01 00:00:00
abstract::Borrelia burgdorferi, the causative agent of Lyme arthritis, does not produce any exported proteases capable of degrading extracellular matrix despite the fact that it is able to disseminate from a skin insertion site to infect multiple organs. Prior studies have shown that B. burgdorferi induces the host protease, ma...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00214-09
更新日期:2009-07-01 00:00:00
abstract::To study responses to Mycobacterium leprae antigens, we developed an in vitro model system in which latex particles coated with M. leprae sonic extract (MLSON) antigen were presented to monocytes. Uptake and oxidative response as measured by superoxide production to these antigens were investigated. Phagocytosis of ML...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.9.3724-3729.1993
更新日期:1993-09-01 00:00:00
abstract::T-cell-mediated immunity is an important determinant in protection against primary infection with Coccidioides immitis, a dimorphic fungal pathogen that causes the disease coccidioidomycosis. To determine if interleukin-12 (IL-12) gene therapy could potentiate host response against C. immitis, we constructed a single-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.6.2996-3001.1999
更新日期:1999-06-01 00:00:00
abstract::Malaria toxin causes hypoglycemia and induction of tumor necrosis factor. Extracts of parasitized erythrocytes which were coeluted and copurified with one of the two subtypes of mammalian insulin-mimetic inositolphosphoglycans similarly induced fibroblast proliferation in the absence of serum. In addition, induction o...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.8.3438-3441.1996
更新日期:1996-08-01 00:00:00
abstract::Legionella pneumophila and Coxiella burnetii are phylogenetically related intracellular bacteria that cause aerosol-transmitted lung infections. In host cells both pathogens proliferate in vacuoles whose biogenesis displays some common features. To test the functional similarity of their respective intracellular niche...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.4494-4504.2005
更新日期:2005-08-01 00:00:00
abstract::A method for stimulating sensitized lymphocytes by inactivated herpes simplex virus was established by using cultures of washed whole blood cells. The development of the immune response of herpes simplex virus-infected guinea pigs was examined at different times in the 4-month period after infection. Humoral and cellu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.3.430-436.1974
更新日期:1974-09-01 00:00:00
abstract::A mutual symbiotic enhancement of growth of Porphyromonas gingivalis and Treponema denticola is described in this report. Brain heart infusion broth supplemented with vitamin K did not support the individual growth of P. gingivalis or T. denticola. However, when inoculated as a mixture, both bacterial species did grow...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.12.5298-5301.1992
更新日期:1992-12-01 00:00:00
abstract::While investigating the requirement for phagosomal alkalinization in the host defense against pulmonary aspergillosis, we observed high morbidity of p47(phox)(-/-) mice infected with pH-insensitive Aspergillus nidulans mutants despite a paucity of fungal growth. Fatal infection also resulted from a normally avirulent ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.5204-5207.2005
更新日期:2005-08-01 00:00:00
abstract::The role of CD8 T cells in controlling Mycobacterium tuberculosis infections in mice was confirmed by comparing the levels of growth of the organism in control, major histocompatibility complex class II knockout, and athymic mice and by transferring T-cell populations into athymic mice. By using donor mice which were ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.2.830-834.1998
更新日期:1998-02-01 00:00:00
abstract::A panel of monoclonal antibodies (MAbs) specific for the heat-stable enterotoxin (STh) of enterotoxigenic Escherichia coli was produced. All four MAbs (8G7, 53-4, 11C, and SH1) bound to native STh in an enzyme-linked immunosorbent assay to various degrees, with clone SH1 showing the best affinity. The MAbs were screen...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.289-294.1993
更新日期:1993-01-01 00:00:00
abstract::Previous reports from our laboratory have shown that 7 days after infection of C3HeB/FeJ mice with an attenuated strain of Salmonella typhimurium, there is profound suppression of responses to B- and T-cell mitogens and suppression of the capacity of spleen cells to mount a primary, in vitro plaque-forming-cell (PFC) ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.9.3786-3792.1996
更新日期:1996-09-01 00:00:00
abstract::Leishmania amastigotes are intracellular protozoan parasites of mononuclear phagocytes which reside within parasitophorous vacuoles of phagolysosomal origin. The pH of these compartments was studied with the aim of elucidating strategies used by these microorganisms to evade the microbicidal mechanisms of their host c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.3.779-787.1990
更新日期:1990-03-01 00:00:00
abstract::The epitope specificities of 13 hybridomas secreting monoclonal antibodies (MAbs) specific for pertussis toxin (PT) is described. Hybridoma lines were derived by the fusion of spleen cells from mice immunized with native PT, Formalin-detoxified PT, or isolated PT subunits (S1 to S5) with the myeloma line X63-Ag8.653. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.9.2660-2665.1989
更新日期:1989-09-01 00:00:00
abstract::Borrelia burgdorferi encodes a functional homolog of canonical Lon protease termed Lon-2. In addition, B. burgdorferi encodes a second Lon homolog called Lon-1. Recent studies suggest that Lon-1 may function differently from the prototypical Lon protease. However, the function of Lon-1 in B. burgdorferi biology remain...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00951-19
更新日期:2020-05-20 00:00:00
abstract::Streptococcal group A, B, and C carbohydrates were analyzed by counterimmunoelectrophoresis, immunoelectrophoresis, and inhibition of immunoprecipitation. Extracts of streptococci group A or C were shown by counterimmunoelectrophoresis to contain both anodic and cathodic migrating components. In immunoelectrophoresis,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.209-215.1982
更新日期:1982-07-01 00:00:00
abstract::Some interrelationships among age, ambient temperature, intestinal transit, and enterotoxigenic Escherichia coli infection were studied in an infant mouse model. The transit of dye in the small intestine was accelerated during the response to heat-stable E. coli enterotoxin. Transit in the small intestine of normal mi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.25.1.127-132.1979
更新日期:1979-07-01 00:00:00