Abstract:
:An attenuated influenza A candidate vaccine virus, derived from the A/Ann Arbor/6/60 (H2N2) cold-adapted (ca) donor virus and the A/Alaska/6/77 (H3N2) wild-type virus, was evaluated in adult seronegative volunteers (serum hemagglutination-inhibiting antibody titer, less than or equal to 1:8) for level of attenuation, infectivity, antigenicity, and genetic stability. Four groups with similar preinoculation mean titers of serum and nasal wash antibodies were inoculated intranasally with 10(4.5), 10(5.5), 10(6.5), or 10(7.5) 50% tissue culture infectious doses (TCID50) of the ca reassortant virus, and eight other seronegative adult volunteers received the wild-type virus. Only 2 of 66 vaccinees developed fever or mild and brief systemic or upper respiratory tract illness or both. Both volunteers with vaccine-related reactions received the highest dose (10(7.5) TCID50) of ca virus, which indicates that the vaccine retains some mild reactogenicity at a high dosage. In contrast, four of eight volunteers infected with the wild-type virus became ill. Each of the 54 isolates tested retained the temperature-sensitive phenotype of the vaccine virus. Thus, the ca reassortant was genetically stable and attenuated at 10(4.5) to 10(7.5) TCID50 for seronegative adults. The 50% human infective dose of ca virus was approximately 10(5.3) TCID50. Ten and one hundred 50% human infectious doses infected 73 and 83% of vaccinees, respectively, and approximately 75% developed an immunological response at these doses. The failure of the vaccine virus to infect some volunteers was correlated with the presence of pre-inoculation nasal wash immunoglobulin A hemagglutinin antibody.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Clements ML,O'Donnell S,Levine MM,Chanock RM,Murphy BRdoi
10.1128/IAI.40.3.1044-1051.1983subject
Has Abstractpub_date
1983-06-01 00:00:00pages
1044-51issue
3eissn
0019-9567issn
1098-5522journal_volume
40pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.1.323-331.1981
更新日期:1981-04-01 00:00:00
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.11.2.355-359.1975
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.46.3.867-869.1984
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pub_type: 杂志文章
doi:10.1128/IAI.55.12.3057-3064.1987
更新日期:1987-12-01 00:00:00
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doi:10.1128/IAI.00135-18
更新日期:2018-06-21 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.9.3638-3643.1997
更新日期:1997-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/IAI.62.5.2051-2057.1994
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.41.2.482-487.1983
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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doi:10.1128/IAI.16.3.817-820.1977
更新日期:1977-06-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.1.118-121.1984
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.2.529-535.1986
更新日期:1986-02-01 00:00:00
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更新日期:2006-12-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
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更新日期:1973-08-01 00:00:00