Abstract:
:Seven cathepsin B-like cysteine proteases (CBLs) were identified from the immunoprotective excretory-secretory products of Haemonchus contortus. Two-dimensional (2-D) zymography and biotinylated inhibitors were employed to localize active CBLs in 2-D protein gels. Mass spectrometry provided the identification of AC-4, HMCP1, HMCP2, and GCP7 as well as three novel CBLs encoded by clustered expressed sequence tags.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Yatsuda AP,Bakker N,Krijgsveld J,Knox DP,Heck AJ,de Vries Edoi
10.1128/IAI.74.3.1989-1993.2006keywords:
subject
Has Abstractpub_date
2006-03-01 00:00:00pages
1989-93issue
3eissn
0019-9567issn
1098-5522pii
74/3/1989journal_volume
74pub_type
杂志文章abstract::A mouse lacking CD28, a T-cell costimulatory molecule, and STAT6, a transcription factor that mediates interleukin-4 (IL-4) signaling, was developed from parental CD28- and STAT6-deficient mice. STAT6/CD28(-/-) BALB/c mice that were 8 weeks old had a normal phenotype, and IL-4 production was induced following infectio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.3706-3715.2004
更新日期:2004-07-01 00:00:00
abstract::Antigens were extracted from a virulent isolate of Pasteurella multocida (serotype 3, 12, 15:D) with potassium thiocyanate, and a vaccine was prepared. Pasteurella-free rabbits were vaccinated intranasally and intraconjuctivally twice with a 2-week interval and challenged intranasally with the homologous P. multocida ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.49.3.498-504.1985
更新日期:1985-09-01 00:00:00
abstract::Mycoplasma arthritidis causes acute polyarthritis in rats and chronic proliferative arthritis in mice. M. arthritidis-induced arthritis serves as a model for arthritis caused by infectious agents and as a model for examining the role of the superantigen MAM (M. arthritidis T-cell mitogen) in the development of autoimm...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.245-249.2005
更新日期:2005-01-01 00:00:00
abstract::Improvement of antibody responses to polysaccharides through their linkage to proteins is thought to be mediated by protein-specific T helper (Th) cells. To investigate whether the carrier protein of a conjugate could be substituted by a Th epitope, Streptococcus pneumoniae type 17F polysaccharide (PS) was bromoacetyl...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.961-968.1995
更新日期:1995-03-01 00:00:00
abstract::The ability of intracellular pathogens to sense and adapt to the hostile environment of the host is an important factor governing virulence. We have sequenced the operon encoding the major heat shock proteins GroES and GroEL in the gram-positive food-borne pathogen Listeria monocytogenes. The operon has a conserved or...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.3924-3932.2001
更新日期:2001-06-01 00:00:00
abstract::Escherichia coli O157:H7, a major Shiga toxin-producing pathogen, has a low infectious dose and causes serious illness in humans. The gastrointestinal tract of cattle is the primary reservoir of E. coli O157:H7, and thus, it is critical to eliminate or reduce E. coli O157:H7 gut colonization. Given that E. coli O157:H...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01589-13
更新日期:2014-05-01 00:00:00
abstract::The Legionella pneumophila major secretory protein (Msp) is a Zn2+ metalloprotease whose function in pathogenesis is unknown. The structural gene for the Msp protease, mspA, was isolated from an L. pneumophila genomic library. In Escherichia coli which contain plasmids with the mspA gene, Msp protein and activity are ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.8.2585-2592.1990
更新日期:1990-08-01 00:00:00
abstract::Gamma interferon (IFN-γ) drives antiparasite responses and immunopathology during infection with Plasmodium species. Immunity-related GTPases (IRGs) are a class of IFN-γ-dependent proteins that are essential for cell autonomous immunity to numerous intracellular pathogens. However, it is currently unknown whether IRGs...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.02701-14
更新日期:2015-04-01 00:00:00
abstract::Culture filtrate proteins from Mycobacterium tuberculosis induce protective immunity in various animal models of tuberculosis. Two molecular mass regions (6 to 10 kDa and 24 to 36 kDa) of short-term culture filtrate are preferentially recognized by Th1 cells in animal models as well as by patients with minimal disease...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.6.2728-2735.1998
更新日期:1998-06-01 00:00:00
abstract::We undertook a morphological and immunochemical comparison of purified outer membrane antigens from oral and nonoral isolates of Bacteroides asaccharolyticus. Electron micrographs of thin sections of whole bacteria revealed a compact, electron-dense capsule external to the outer membrane of oral strains. A loose, web-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.589-596.1980
更新日期:1980-02-01 00:00:00
abstract::Gamma interferon (IFN-gamma)-activated macrophages use an alternative processing mechanism to present Salmonella antigens to CD8(+) T lymphocytes. This pathway involves processing of antigen in a vacuolar compartment followed by secretion and loading of antigenic peptides to major histocompatibility complex class I (M...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.7.3937-3944.2005
更新日期:2005-07-01 00:00:00
abstract::Antigen-presenting cells (APCs) are key players in the induction and regulation of immune responses. In Plasmodium falciparum malaria, determination of which cells and pathways are activated in the network of APCs remains elusive. We therefore investigated the effects of a controlled human malaria infection in healthy...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00473-15
更新日期:2015-09-01 00:00:00
abstract::In order to plan for the wide-scale introduction of meningococcal C conjugate (MCC) vaccine for United Kingdom children up to 18 years old, phase II trials were undertaken to investigate whether there was any interaction between MCC vaccines conjugated to tetanus toxoid (TT) or a derivative of diphtheria toxin (CRM(19...
journal_title:Infection and immunity
pub_type: 临床试验,杂志文章
doi:10.1128/iai.70.9.4946-4954.2002
更新日期:2002-09-01 00:00:00
abstract::Recent epidemiological observations suggest that clinical evolution of Plasmodium falciparum infections might be influenced by the concurrent presence of another Plasmodium species, and such mixed-species infections are now known to occur frequently in residents of most areas of endemicity. We used mice infected with ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.8.4777-4786.2005
更新日期:2005-08-01 00:00:00
abstract::Immunization of BALB/c mice with a plasmid containing the gene for Trypanosoma cruzi trans-sialidase (TS) induced antibodies that inhibited TS enzymatic activity, CD4+ Th1 and CD8+ Tc1 cells, and protective immunity against infection. We used this model to obtain basic information on the requirement of CD4 or CD8 or B...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.69.9.5477-5486.2001
更新日期:2001-09-01 00:00:00
abstract::A fibronectin (Fn)-binding adhesin of Staphylococcus aureus contains three tandem 37- or 38-amino-acid motifs (D1, D2, and D3), which function to bind Fn. Plasma from patients with S. aureus infections contain antibodies that preferentially recognize ligand induced binding sites in the D motifs and do not inhibit Fn b...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1156-1163.2000
更新日期:2000-03-01 00:00:00
abstract::After lysing the phagocytic vacuole, Shigella spp. accumulate filaments of polymerized actin on their surface at one pole, leading to the formation of actin tails that enable them to move through the cytoplasm. We have recently demonstrated that the Shigella protein IcsA is located at the pole that is adjacent to the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.12.5664-5668.1994
更新日期:1994-12-01 00:00:00
abstract::Enteropathogenic strains of Yersinia enterocolitica harbor a virulence plasmid (70 kilobases) which specifies, at 37 degrees C, a calcium requirement for growth, autoagglutinability, resistance to the bactericidal activity of human serum, and the expression of some outer membrane proteins (OMPs). To map the genes enco...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.782-786.1985
更新日期:1985-06-01 00:00:00
abstract::In experimental visceral leishmaniasis, in which the tissue macrophage is the target, in vivo responsiveness to conventional chemotherapy (pentavalent antimony [Sb]) requires a T-cell-dependent mechanism. To determine if this mechanism involves gamma interferon (IFN-gamma)-induced activation and/or specific IFN-gamma-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.1.288-293.2000
更新日期:2000-01-01 00:00:00
abstract::Ehrlichia chaffeensis, a tick-transmitted rickettsial, is the causative agent of human monocytic ehrlichiosis. To examine protein expression patterns, we analyzed total, membrane, and immunogenic proteomes of E. chaffeensis originating from macrophage and tick cell cultures. Total proteins resolved by one-dimensional ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00484-08
更新日期:2008-11-01 00:00:00
abstract::Haemophilus ducreyi previously has been shown to inhibit the phagocytosis of both secondary targets and itself by certain cells in vitro. Wild-type H. ducreyi strain 35000HP contains two genes, lspA1 and lspA2, whose encoded protein products are predicted to be 456 and 543 kDa, respectively. An isogenic mutant of H. d...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.10.5994-6003.2003
更新日期:2003-10-01 00:00:00
abstract::Previous passive antibody transfer experiments have indicated that immunity to a 46-kilodalton membrane glycoprotein (M-2) of Leishmania amazonensis may protect against infection with this parasite. In the studies described in this paper, we investigated the ability of the purified M-2 molecule to elicit a protective ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.12.3272-3279.1988
更新日期:1988-12-01 00:00:00
abstract::Malaria in pregnancy is associated with placental accumulation of Plasmodium falciparum-infected erythrocytes (IE) that adhere to chondroitin sulfate A (CSA). Adhesion is mediated by P. falciparum erythrocyte membrane protein 1 (PfEMP1), a variant parasite protein expressed on the surface of IE and encoded by var gene...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.5.2848-2856.2005
更新日期:2005-05-01 00:00:00
abstract::We have previously demonstrated that fibronectin mediates streptococcal adhesion to host cells and that streptococci interact primarily with the N-terminal domain of fibronectin. FBP54 is a 54-kDa protein from group A streptococci that binds fibronectin. In this report, we show that the N-terminal domain of fibronecti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.7.2415-2419.1996
更新日期:1996-07-01 00:00:00
abstract::To analyze the role of interleukin-10 (IL-10) in bacterial cerebral infections, we studied cerebral listeriosis in IL-10-deficient (IL-10(-/-)) and wild-type (WT) mice, the latter of which express high levels of IL-10 in both primary and secondary cerebral listeriosis. IL-10(-/-) mice succumbed to primary as well as s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4561-4571.2001
更新日期:2001-07-01 00:00:00
abstract::Peptidoglycans of organisms belonging to the strictly anaerobic family Bacteroidaceae were investigated for the presence of lanthionine. Different procedures for the quantitation of lanthionine were compared. Performic acid and peroxide oxidation procedures on 35S-labeled peptidoglycan from Fusobacterium nucleatum Fev...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.36.1.114-122.1982
更新日期:1982-04-01 00:00:00
abstract::Adult volunteers received a booster dose (4 x 10(8) CFU) of attenuated Vibrio cholerae CVD 103-HgR oral vaccine 15 or 24 months after primary immunization. The immune response was modest, presumably due to rapid clearance of the vaccine strain by a primed immune system. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.9.3916-3917.1992
更新日期:1992-09-01 00:00:00
abstract::Chagas' disease is a chronic infection caused by Trypanosoma cruzi and represents an important public health burden in Latin America. Frequently the disease evolves undetectable for decades, while in a significant fraction of the affected individuals it culminates in death by heart failure. Here, we describe a novel m...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.72.4.2350-2357.2004
更新日期:2004-04-01 00:00:00
abstract::Stenotrophomonas maltophilia is a ubiquitous bacterium and an emerging nosocomial pathogen. This bacterium is resistant to many antibiotics, associated with a number of infections, and a significant health risk, especially for immunocompromised patients. Given that Caenorhabditis elegans shares many conserved genetic ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00711-15
更新日期:2015-12-07 00:00:00
abstract::Antigen preparations derived from Plasmodium falciparum-infected erythrocytes (but not from uninfected erythrocytes) can stimulate the in vitro proliferation of peripheral blood lymphocytes from malaria-sensitized as well as nonsensitized donors. The possibility that the nonspecific responses might be due to a parasit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.24.1.106-110.1979
更新日期:1979-04-01 00:00:00