Abstract:
:Gamma interferon (IFN-γ) drives antiparasite responses and immunopathology during infection with Plasmodium species. Immunity-related GTPases (IRGs) are a class of IFN-γ-dependent proteins that are essential for cell autonomous immunity to numerous intracellular pathogens. However, it is currently unknown whether IRGs modulate responses during malaria. We have used the Plasmodium berghei ANKA (PbA) model in which mice develop experimental cerebral malaria (ECM) to study the roles of IRGM1 and IRGM3 in immunopathology. Induction of mRNA for Irgm1 and Irgm3 was found in the brains and spleens of infected mice at times of peak IFN-γ production. Irgm3-/- but not Irgm1-/- mice were completely protected from the development of ECM, and this protection was associated with the decreased induction of inflammatory cytokines, as well as decreased recruitment and activation of CD8+ T cells within the brain. Although antigen-specific proliferation of transferred CD8+ T cells was not diminished compared to that of wild-type recipients following PbA infection, T cells transferred into Irgm3-/- recipients showed a striking impairment of effector differentiation. Decreased induction of several inflammatory cytokines and chemokines (interleukin-6, CCL2, CCL3, and CCL4), as well as enhanced mRNA expression of type-I IFNs, was found in the spleens of Irgm3-/- mice at day 4 postinfection. Together, these data suggest that protection from ECM pathology in Irgm3-/- mice occurs due to impaired generation of CD8+ effector function. This defect is nonintrinsic to CD8+ T cells. Instead, diminished T cell responses most likely result from defective initiation of inflammatory responses in myeloid cells.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Guo J,McQuillan JA,Yau B,Tullo GS,Long CA,Bertolino P,Roediger B,Weninger W,Taylor GA,Hunt NH,Ball HJ,Mitchell AJdoi
10.1128/IAI.02701-14subject
Has Abstractpub_date
2015-04-01 00:00:00pages
1406-17issue
4eissn
0019-9567issn
1098-5522pii
IAI.02701-14journal_volume
83pub_type
杂志文章abstract::The 25-kb linear plasmid lp25 and one of the 28-kb linear plasmids (lp28-1) are required for experimental infection in Borrelia burgdorferi, the etiologic agent of Lyme disease. The loss of these plasmids either eliminates infectivity (lp25) or significantly increases the 50% infective dose during a 2-week infection p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.8.4608-4613.2003
更新日期:2003-08-01 00:00:00
abstract::Helicobacter pylori maintains colonization in its human host using a limited set of taxis sensors. TlpD is a proposed energy taxis sensor of H. pylori and dominant under environmental conditions of low bacterial energy yield. We studied the impact of H. pylori TlpD on colonization in vivo using a gerbil infection mode...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00750-13
更新日期:2013-10-01 00:00:00
abstract::Streptococcus suis capsular type 2 is an important etiological agent of swine meningitis, and it is also a zoonotic agent. Since one hypothesis of the pathogenesis of S. suis infection is that bacteria enter the bloodstream and invade the meninges and other tissues in close association with mononuclear phagocytes, the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.8.4312-4322.2002
更新日期:2002-08-01 00:00:00
abstract::A fibronectin-binding protein (FnBP) adhesin of Staphylococcus aureus possesses three 37- or 38-amino-acid motifs (D1, D2, and D3) that can each bind fibronectin (Fn) with low affinity and that in tandem comprise D1-3, a high-affinity Fn-binding domain. To identify epitopes for the generation of adhesion-blocking anti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.2.537-543.1997
更新日期:1997-02-01 00:00:00
abstract::We reexamined the binding specificity of the Shiga-like toxin variant associated with porcine edema disease, SLT2e, which is reported to be more cytotoxic for Vero cells than for HeLa cells, by using receptor-deficient cells and a liposomal insertion system for purified glycolipids. We found that SLT2e preferentially ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.3.1138-1141.1995
更新日期:1995-03-01 00:00:00
abstract::Enterococcus faecalis strains are resident intestinal bacteria associated with invasive infections, inflammatory bowel diseases, and colon cancer. Although factors promoting E. faecalis colonization of intestines are not fully known, one implicated pathway is a phosphotransferase system (PTS) in E. faecalis strain OG1...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00080-19
更新日期:2019-06-20 00:00:00
abstract::Immunoglobulin G (IgG) antibodies against hepatitis B surface antigen were found to be restricted to subclasses IgG1 and IgG3 in serum samples of 17 individuals. Quantitative determinations showed that in 10 samples the minor serum subclass IgG3 contained more antibodies than the predominant subclass IgG1. Ranges of c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.39.2.565-568.1983
更新日期:1983-02-01 00:00:00
abstract::C57BL/6 mice were infected intravenously with 6 X 10(3) Listeria monocytogenes organisms. As early as day 3 of infection, there was a marked reduction in the number of lymphocytes recovered from the peripheral blood, bone marrow, and thymuses of infected animals. Concomitantly, there was an increase in the number of s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.45.2.372-377.1984
更新日期:1984-08-01 00:00:00
abstract::Despite the autotransporter (AT) moniker, AT secretion appears to involve the function of periplasmic chaperones. We identified four periplasmic proteins that specifically bound to plasmid-encoded toxin (Pet), an AT produced by enteroaggregative Escherichia coli (EAEC). These proteins include the 17-kDa Skp chaperone ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00167-12
更新日期:2012-07-01 00:00:00
abstract::To elucidate the mechanism of the high incidence of lower respiratory tract infections in patients with diabetes mellitus, we investigated the kinetics of production of macrophage inflammatory protein 2 (MIP-2), an important mediator of lung neutrophil recruitment, using mice with streptozotocin-induced diabetes. Intr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.5.2925-2929.2000
更新日期:2000-05-01 00:00:00
abstract::The gastric spirillum Helicobacter felis, originally isolated from the cat stomach, colonizes the stomachs of germfree rats. Studies were designed to examine the pathological and serological responses of germfree rats inoculated orally with H. felis. At 2 weeks postinoculation, the gastric mucosa of germfree rats had ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.3.785-791.1991
更新日期:1991-03-01 00:00:00
abstract::The bacterium Neisseria meningitidis is the causative agent of meningitis and sepsis. A generally effective vaccine against N. meningitidis serogroup B is not yet available, but outer membrane vesicle vaccines are in development. These vaccines contain lipopolysaccharide (LPS). The inclusion of N. meningitidis wild-ty...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00846-07
更新日期:2007-12-01 00:00:00
abstract::A method was developed to study the adhesion of Streptococcus pneumoniae to human pharyngeal epithelial cells. Epithelial cells from healthy persons, pneumococcal strains from patients with otitis media, meningitis, or septicemia, and pneumococcal cells from the nasopharynx of healthy carriers were used. Adhesion was ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.32.1.311-317.1981
更新日期:1981-04-01 00:00:00
abstract::Knowledge of whether Mycobacterium tuberculosis resides within a relatively impermeable membrane-bound vacuole or is free within the cytoplasm within its host cell is central to an understanding of the immunobiology of this intracellular parasite but is a matter of controversy. To explore this issue, we assessed the a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.70.10.5800-5807.2002
更新日期:2002-10-01 00:00:00
abstract::We show here that Helicobacter pylori broth culture supernatants disrupt the actin cytoskeleton of epithelial cell lines, leading to cell rounding and apoptosis through anoikis. We demonstrate that there are marked quantitative differences between strains and that there are different cell line sensitivities. By constr...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.6.3623-3627.2003
更新日期:2003-06-01 00:00:00
abstract::The interactions between Klebsiella pneumoniae and the host environment at the site of infection are largely unknown. Pulmonary surfactant serves as an initial point of contact for inhaled bacteria entering the lung and is thought to contain molecular cues that aid colonization and pathogenesis. To gain insight into t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00135-18
更新日期:2018-06-21 00:00:00
abstract::Analysis of the specificity of bactericidal antibodies in normal, convalescent, and postvaccination human sera is important in understanding human immunity to meningococcal infections and can aid in the design of an effective group B vaccine. A collection of human sera, including group C and group B convalescent-phase...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05125-11
更新日期:2011-10-01 00:00:00
abstract::The gene products from an 8-kb region adjacent to the 3' end of the ptx operon are required by Bordetella pertussis for the export of pertussis holotoxin. At least one of these gene products (PtlC) is specifically required for the export of assembled holotoxin from the periplasmic space. ptlC mutants exhibit a 20-fold...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.10.3920-3926.1995
更新日期:1995-10-01 00:00:00
abstract::Protection against infection with virulent blood (trypomastigote) forms of Trypanosoma cruzi was accomplished in mice by immunization with culture (mainly epimastigote) forms killed by treatment with sodium perchlorate. Sodium chloride, used instead of sodium perchlorate, with all other conditions kept the same, faile...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.12.3.461-465.1975
更新日期:1975-09-01 00:00:00
abstract::Previous studies have suggested that the Pseudomonas aeruginosa PAK pilus adhesin moiety resides in an epithelial cell-binding domain located in the C-terminal region of the PAK pilin structural protein. Synthetic peptides Ac17red (a synthetic peptide with a sequence identical to that of PAK pilin residues 128 to 144,...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.12.3720-3726.1989
更新日期:1989-12-01 00:00:00
abstract::The adherence mechanism of Aeromonas caviae Sch3N to HEp-2 cells was initially investigated through four mini-Tn5 mutants that showed a 10-fold decrease in adherence. These mutants lost motility, flagella, and their lipopolysaccharide (LPS) O antigen (O-Ag). Three genes, flmB-neuA-flmD, were found to be interrupted by...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.65-74.2001
更新日期:2001-01-01 00:00:00
abstract::Salmonella typhimurium SH5014 porins induce the release of tumor necrosis factor alpha (TNF-alpha), interleukin 1 alpha (IL-1 alpha), and IL-6 by human monocytes and of gamma interferon (IFN-gamma) and IL-4 by human lymphocytes. Porins at 1 microgram/ml induce the greatest release of TNF-alpha, IL-1 alpha, and IL-6 by...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.1.155-161.1993
更新日期:1993-01-01 00:00:00
abstract::Group A streptococci (GAS) are highly prevalent human pathogens whose primary ecological niche is the superficial epithelial layers of the throat and/or skin. Many GAS strains with a strong tendency to cause pharyngitis are distinct from strains that tend to cause impetigo; thus, genetic differences between them may c...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00374-17
更新日期:2017-10-18 00:00:00
abstract::The possibility that preexisting specific serum antibody could suppress a defined mucosal immune response to a topically applied antigen was studied in rats. Hyperimmune serum antibody induced by parenteral immunization of rats with cholera toxoid markedly suppressed the mucosal immune response to enterically applied ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.30.1.62-68.1980
更新日期:1980-10-01 00:00:00
abstract::We report the cloning and expression of Ac-GST-1, a novel glutathione S-transferase from the adult hookworm Ancylostoma caninum, and its possible role in parasite blood feeding and as a vaccine target. The predicted Ac-GST-1 open reading frame contains 207 amino acids (mass, 24 kDa) and exhibited up to 65% amino acid ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.10.6903-6911.2005
更新日期:2005-10-01 00:00:00
abstract::Elevated levels of immunoglobulin E anti-Candida antibodies were observed in the sera of patients with systemic and vaginal candidiasis and in cervicovaginal washings of the latter. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.1.257-259.1977
更新日期:1977-10-01 00:00:00
abstract::Legionella pneumophila multiplied rapidly in guinea pig and rat alveolar macrophages but failed to grow when phagocytic activity was inhibited by pretreatment with 0.5 or 1.0 microgram of cytochalasin D per ml. Attachment was not inhibited by cytochalasin D. No extracellular multiplication occurred when L. pneumophila...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.51.1.31-36.1986
更新日期:1986-01-01 00:00:00
abstract::The contribution of murine Toll-like receptors 2 and 4 (TLR2 and -4, respectively) to cytokine induction by heat-killed bacteria was analyzed in vitro and in vivo. Gram-negative bacteria induced cytokines primarily via TLR4; the contribution of TLR2 was only minor. Neither TLR4 nor, surprisingly, TLR2 was required in ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.10.6058-6062.2003
更新日期:2003-10-01 00:00:00
abstract::Mouse spleen cultures were infected with murine cytomegalovirus, either before or after separating the cells into various classes, and the growth of virus and production of infectious centers were measured in each cell class. The separation techniques, which were used in various combinations, comprised: adherence to p...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.853-860.1979
更新日期:1979-12-01 00:00:00
abstract::The Lyme disease agent, Borrelia burgdorferi, is able to persistently infect humans and animals for months or years in the presence of an active immune response. It is not known how the organisms survive immune attack in the mammalian host. vlsE, a gene localized near one end of linear plasmid lp28-1 and encoding a su...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.8.3689-3697.1998
更新日期:1998-08-01 00:00:00