Abstract:
:To elucidate the mechanism of the high incidence of lower respiratory tract infections in patients with diabetes mellitus, we investigated the kinetics of production of macrophage inflammatory protein 2 (MIP-2), an important mediator of lung neutrophil recruitment, using mice with streptozotocin-induced diabetes. Intratracheal challenge with 1 mg of lipopolysaccharide (LPS), an endotoxin, per kg of body weight resulted in a time-dependent increase in the levels of MIP-2 protein in bronchoalveolar lavage (BAL) fluid, with the peak concentration (49.4 +/- 13 ng/ml) occurring at 3 h and significant neutrophil accumulation becoming apparent by 3 h in normal mice. In diabetic mice, the peak level of MIP-2 protein in BAL fluid did not occur until 6 h and was reduced to 21.9 +/- 10 ng/ml. Immunohistochemical studies using anti-MIP-2 antibody confirmed that the main cellular source of MIP-2 in the lung after LPS challenge was alveolar macrophages (AMs) in normal mice. The lungs in diabetic mice, however, showed no AMs staining for MIP-2 within 3 h after LPS challenge. PCR analysis using whole-lung RNA showed a time-dependent increase in MIP-2 mRNA levels after LPS instillation. The level of MIP-2 mRNA in diabetic mice was markedly decreased compared to that in normal mice. Our results indicate that impairment of MIP-2 mRNA expression in the AMs in diabetic mice resulted in delayed neutrophil recruitment in the lungs, and this may explain the development and progression of pulmonary infection in diabetes mellitus.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Amano H,Yamamoto H,Senba M,Oishi K,Suzuki S,Fukushima K,Mukaida N,Matsushima K,Eguchi K,Nagatake Tdoi
10.1128/iai.68.5.2925-2929.2000keywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
2925-9issue
5eissn
0019-9567issn
1098-5522journal_volume
68pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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更新日期:1984-03-01 00:00:00
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journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.34.1.177-183.1981
更新日期:1981-10-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1986-02-01 00:00:00
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更新日期:2019-01-24 00:00:00
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journal_title:Infection and immunity
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更新日期:2000-04-01 00:00:00
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journal_title:Infection and immunity
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更新日期:1997-09-01 00:00:00