Abstract:
:Sublethal infection of mice with recombinant Listeria monocytogenes expressing a model epitope in either secreted or nonsecreted form results in similar CD8(+) T-cell priming. Since nonsecreted bacterial proteins have no obvious access to the endogenous major histocompatibility complex (MHC) class I presentation pathway, presentation of these antigens requires destruction of the bacterium to reveal the nonsecreted molecules to an exogenous MHC class I presentation pathway. Gamma interferon (IFN-gamma), a cytokine made by multiple cell types in response to L. monocytogenes infection, could be required for exogenous presentation of nonsecreted bacterial antigens via its capacity to upregulate the expression of molecules involved in antigen presentation, its capacity to activate macrophages to kill bacteria to expose nonsecreted molecules or both. IFN-gamma knockout (KO) mice were used to address the requirement for IFN-gamma in CD8(+) T-cell priming against (i) a model exogenous antigen and (ii) secreted and nonsecreted L. monocytogenes antigens. We demonstrate that IFN-gamma KO mice are capable of cross-presenting the model exogenous antigen ovalbumin to prime CD8(+) T-cell responses that are only slightly weaker than that in wild-type (WT) mice. Despite their extreme susceptibility to primary L. monocytogenes infection, previously immunized and naive IFN-gamma KO mice were able to generate CD8(+) T-cell responses against both secreted and nonsecreted L. monocytogenes antigens which were similar to responses of WT mice. Interestingly, IFN-gamma KO mice were as capable as WT mice in mediating the characteristic drop in bacterial load in the liver at 4 h postinfection, although the IFN-gamma KO mice have exacerbated bacterial loads as early as 24 h postinfection. These results demonstrate that the regulatory functions of IFN-gamma are not required for priming of CD8(+) T cells by cross-presentation of a model exogenous antigen or in response to a nonsecreted L. monocytogenes antigen. In addition, the capacity of IFN-gamma to activate the microbicidal activities of macrophages is not required for the very early innate immune response to L. monocytogenes or priming of CD8(+) T cells against a nonsecreted bacterial antigen.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Tvinnereim AR,Harty JTdoi
10.1128/iai.68.4.2196-2204.2000keywords:
subject
Has Abstractpub_date
2000-04-01 00:00:00pages
2196-204issue
4eissn
0019-9567issn
1098-5522journal_volume
68pub_type
杂志文章abstract::FMS-like tyrosine kinase-3 ligand (Flt3L) is a dendritic cell (DC) growth and differentiation factor with potential in antitumor therapies and antibacterial immunization strategies. However, the effect of systemic Flt3L treatment on lung-protective immunity against bacterial infection is incompletely defined. Here, we...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00854-12
更新日期:2012-12-01 00:00:00
abstract::The effects of pathologically relevant concentrations (0.38 to 12.5 microM) of the proinflammatory, Pseudomonas aeruginosa-derived pigment 1-hydroxyphenazine (1-hp) on Ca2+ metabolism and intracellular cyclic AMP (cAMP) in N-formyl-L-methionyl-L-leucyl-L-phenylalanine (FMLP; 1 microM)-activated human neutrophils, as w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5157-5162.1999
更新日期:1999-10-01 00:00:00
abstract::Chronic actinomycetoma associated with grain production was induced in BALB/c mice by subcutaneous inoculation of live Nocardia brasiliensis in Freund incomplete adjuvant into the hind footpads. Similar inoculation of N. asteroides and N. caviae resulted in local tumor formation which healed spontaneously after 5 mont...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1980-09-01 00:00:00
abstract::Recent work has demonstrated that the polyketide natural product Aurodox from Streptomyces goldiniensis is able to block the pathogenesis of the murine pathogen Citrobacter rodentium In this work, we aimed to gain a better understanding of the mechanism of action of the compound. We show that Aurodox downregulates the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00595-18
更新日期:2019-01-24 00:00:00
abstract::Because combined administration of intramuscular and intravenous interferon has been partially successful in the incubationary treatment of rabies, the effect of direct interferon administration into the cerebrospinal fluid space was tested. After injecting 1,800 U of interferon into the cisterna magna or the lateral ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.9.2.286-293.1974
更新日期:1974-02-01 00:00:00
abstract::Active immunization with Escherichia coli-expressed recombinant outer surface protein C (OspC) of Borrelia burgdorferi has been demonstrated to confer protection against a tick-transmitted infection on laboratory animals. A previous study in this laboratory showed that OspC antibody raised against a denatured immunoge...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.10.5463-5469.1999
更新日期:1999-10-01 00:00:00
abstract::Slime production is not a generally recognized feature of Staphylococcus epidermidis. In a recent outbreak of S. epidermidis intravascular catheter-associated sepsis, we noted that 63% of clinically implicated strains grew as a slimy film coating the culture tube walls when propagated in tryptic soy broth. Only 37% of...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.37.1.318-326.1982
更新日期:1982-07-01 00:00:00
abstract::The stress-induced extracytoplasmic sigma factor E (SigE) of Mycobacterium tuberculosis shows increased expression after heat shock, sodium dodecyl sulfate treatment, and oxidative stress, as well as after phagocytosis in macrophages. We report that deletion of sigE results in delayed lethality in mice without a signi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.7170-7172.2003
更新日期:2003-12-01 00:00:00
abstract::Gram-negative organisms incorporate hydroxy fatty acids into the lipid A moiety of lipopolysaccharide (LPS), and in the case of some members of the family Enterobacteriaceae, hydroxy fatty acids are incorporated exclusively into lipid A. However, a limited number of Bacteroides species have been shown to incorporate s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.62.9.3753-3760.1994
更新日期:1994-09-01 00:00:00
abstract::We previously identified a cell wall-associated protein from Fusobacterium nucleatum, a Gram-negative bacterium of the oral cavity, that induces human beta defensin 2 (hBD-2) in primary human oral epithelial cells (HOECs) and designated it FAD-I (Fusobacterium-associated defensin inducer). Here, we report differential...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01311-15
更新日期:2016-04-22 00:00:00
abstract::The interactions between Capnocytophaga ochracea ATCC 33596 and Streptococcus sanguis H1, Actinomyces naeslundii PK984, or Actinomyces israelii PK16 are dependent on specific recognitions between heat-sensitive adhesins on C. ochracea and heat-stable structures (probably carbohydrate-containing receptors) on the surfa...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.5.1198-1202.1987
更新日期:1987-05-01 00:00:00
abstract::A large-scale DNA vaccination trial was performed with sheep to investigate whether an antigen targeted by CTLA-4 enhanced and accelerated the humoral immune response. Vaccination with genetically detoxified phospholipase D (DeltaPLD) has been shown to be effective, at least partially, against Corynebacterium pseudotu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:
更新日期:1999-12-01 00:00:00
abstract::Moraxella catarrhalis is a causative agent of otitis media in children and lower respiratory tract infections in adults suffering from chronic obstructive pulmonary disease (COPD). This strict human pathogen continues to be a significant cause of disease in this broad spectrum of patients because there is no available...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00294-09
更新日期:2009-10-01 00:00:00
abstract::The two-component regulatory system, GraRS, appears to be involved in staphylococcal responses to cationic antimicrobial peptides (CAPs). However, the mechanism(s) by which GraRS is induced, regulated, and modulated remain undefined. In this study, we used two well-characterized MRSA strains (Mu50 and COL) and their r...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.05669-11
更新日期:2012-01-01 00:00:00
abstract::Actinobacillus pleuropneumoniae serotype 7 strains are shown to spontaneously lose cytolytic activity with a frequency of approximately 10(-4). The phenotypic change is associated with the loss of approximately 8.5 kbp of chromosomal DNA. A genomic fragment encoding the cytolysin and its flanking sequences was cloned ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.59.11.4110-4116.1991
更新日期:1991-11-01 00:00:00
abstract::Ring-necked pheasant virus, an avian leukosis virus, when injected into 10-day old chick embryos, caused angiosarcomas in the lungs of infected chickens within a short time. Angiosarcomas appeared as localized foci of proliferating cells in the lungs as early as 2 weeks posthatch, and by 6 weeks, the lungs of the infe...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.40.1.310-319.1983
更新日期:1983-04-01 00:00:00
abstract::Sera from adult periodontitis patients were analyzed for antibody activity against surface antigens of Bacteroides gingivalis by immunoblotting analysis. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.55.3.832-834.1987
更新日期:1987-03-01 00:00:00
abstract::A gene, dmcA, expressing a methyl-accepting chemotaxis protein (MCP) from the oral spirochete Treponema denticola has been characterized. The gene was initially identified as an open reading frame immediately upstream from the previously characterized prtB protease gene of strain ATCC 35405. Nucleotide sequencing of t...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.65.10.4011-4016.1997
更新日期:1997-10-01 00:00:00
abstract::Colony variants of Neisseria gonorrhoeae differ in their interactions with eucaryotic cells. When gonococci were cultivated with HeLa cell monolayers, the opacity phenotype (Op) became increasingly dominant in the subpopulation of organisms which adhered to the HeLa cells. Once bound, Op organisms displayed very low l...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.54.1.154-160.1986
更新日期:1986-10-01 00:00:00
abstract::Subtilase cytotoxin (SubAB) was first isolated from a Shiga toxigenic Escherichia coli (STEC) strain that was responsible for an outbreak of hemolytic-uremic syndrome and is the prototype of a new family of AB(5) cytotoxins. SubAB is a subtilase-like serine protease, and upon uptake by host cells, it is trafficked to ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00801-10
更新日期:2010-11-01 00:00:00
abstract::The smooth lipopolysaccharide complex of the outer surface of smooth Brucella abortus cells is believed to be the antigenic component involved in serological tests routinely used for the diagnosis of brucellosis. Sera from cattle vaccinated or infected with B. abortus generally contain antibody directed toward the smo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.21.3.994-1002.1978
更新日期:1978-09-01 00:00:00
abstract::Ultrastructural changes in cells of the mouse footpad are described which occurred during the log phase of multiplication, the plateau, and the stationary phase of growth of Mycobacterium leprae.BALB/c mice were inoculated in the right hind footpad with 5 x 10(3) organisms and sacrificed in pairs at 86 to 173 days aft...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.2.238-247.1972
更新日期:1972-02-01 00:00:00
abstract::Streptolysin O (SLO) and streptolysin S (SLS) are potent cytolytic toxins produced by almost all clinical isolates of group A streptococci (GAS). Allele-replacement mutagenesis was used to construct nonpolar (in-frame) deletion mutations in the slo and sagB genes of the serotype M5 GAS strain Manfredo, producing isoge...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.3857-3865.2003
更新日期:2003-07-01 00:00:00
abstract::Meningococcal sodC encodes periplasmic copper- and zinc-cofactored superoxide dismutase (Cu,Zn SOD) which catalyzes the conversion of the superoxide radical anion to hydrogen peroxide, preventing a sequence of reactions leading to production of toxic hydroxyl free radicals. From its periplasmic location, Cu,Zn SOD was...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.1.213-217.1998
更新日期:1998-01-01 00:00:00
abstract::Recent studies revealed that acetylation is a widely used protein modification in prokaryotic organisms. The major protein acetylation acetyltransferase YfiQ and the sirtuin-like deacetylase CobB have been found to be involved in basic physiological processes, such as primary metabolism, chemotaxis, and stress respons...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00224-18
更新日期:2018-05-22 00:00:00
abstract::Human serum at low concentrations inhibits the growth of Cryptococcus neoformans in vitro. Fractionation of serum yielded a purified inhibitory protein with a molecular mass of approximately 81.8 kDa, a pI of approximately 6.2, and an amino acid sequence that matched that of human transferrin. The inhibitory activity ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.6.3787-3791.2000
更新日期:2000-06-01 00:00:00
abstract::Numerous bacterial pathogens use type III secretion systems (T3SSs) or T4SSs to inject or translocate virulence proteins into eukaryotic cells. Several different reporter systems have been developed to measure the translocation of these proteins. In this study, a peptide tag-based reporter system was developed and use...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00690-06
更新日期:2006-10-01 00:00:00
abstract::Antigens extracted from cells of Streptococcus pyogenes T6 and Streptococcus mutans strains AHT, BHT, 10449, OMZ175, and K1R adsorbed to the sarcolemmal sheath of cardiac muscle cells in vitro. Similar preparations from S. salivarius, S. sanguis, Staphylococcus aureus, and Lactobacillus casei had weak or negligible ti...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.604-613.1980
更新日期:1980-02-01 00:00:00
abstract::Neisseria gonorrhoeae enters cultured human mucosal cells following binding of a distinct gonococcal opacity (Opa) outer membrane protein to cell surface proteoglycan receptors. We examined the route of internalization that is activated by Opa-expressing gonococci (strain VP1). Microscopy of infected Chang epithelial ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.5.1621-1630.1996
更新日期:1996-05-01 00:00:00
abstract::Assembly of adherent biofilms is the key mechanism involved in Staphylococcus epidermidis virulence during device-associated infections. Aside from polysaccharide intercellular adhesin (PIA), the accumulation-associated protein Aap and the extracellular matrix binding protein Embp act as intercellular adhesins, mediat...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01142-10
更新日期:2011-06-01 00:00:00