Abstract:
:Slime production is not a generally recognized feature of Staphylococcus epidermidis. In a recent outbreak of S. epidermidis intravascular catheter-associated sepsis, we noted that 63% of clinically implicated strains grew as a slimy film coating the culture tube walls when propagated in tryptic soy broth. Only 37% of randomly collected blood culture contaminants and skin isolates demonstrated a similar phenomenon (p less than 0.05). Transmission electron micrographs of these coating bacteria showed them to be encased in an extracellular matrix that stained with alcian blue. Slime production was most evident in autoclaved media containing Casamino Acids and glucose supplementation (0.25% wt/vol). There were strain and media preparation variability of slime production in the presence of other carbohydrates. Some strains were not able to produce slime under any of the tested conditions. The production or nonproduction of slime did not influence growth rate. When grown in vitro, slime producers accumulated on the surface of intravascular catheters as macrocolonies, whereas non-slime, producers did not. Transmission and scanning electron micrographs showed slime producers to be encased in an adhesive layer on the catheter surface, whereas nonproducers were not encased. These results suggest that slime-mediated adherence may be a critical factor in the pathogenesis of S. epidermidis infections of medical devices.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Christensen GD,Simpson WA,Bisno AL,Beachey EHdoi
10.1128/IAI.37.1.318-326.1982subject
Has Abstractpub_date
1982-07-01 00:00:00pages
318-26issue
1eissn
0019-9567issn
1098-5522journal_volume
37pub_type
杂志文章abstract::Hepatitis, proliferative typhlitis, and colitis were characterized in young adult and older SCID/NCr mice naturally infected with Helicobacter hepaticus. Liver lesions consisted of Kupffer, Ito, and oval cell hyperplasia along with multifocal to coalescing coagulative hepatocyte necrosis. Numerous Warthin-Starry-posit...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.11.5477-5484.1998
更新日期:1998-11-01 00:00:00
abstract::The ability of intracellular pathogens to sense and adapt to the hostile environment of the host is an important factor governing virulence. We have sequenced the operon encoding the major heat shock proteins GroES and GroEL in the gram-positive food-borne pathogen Listeria monocytogenes. The operon has a conserved or...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.6.3924-3932.2001
更新日期:2001-06-01 00:00:00
abstract::Feline leukemia-sarcoma group-specific antigens were located in human embryo cells infected with feline leukemia and feline sarcoma viruses. This was done by using the fluorescent-antibody and enzyme-labeled antibody techniques at both light and electron microscopic levels. The antigens were found to be exclusively in...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.5.3.400-405.1972
更新日期:1972-03-01 00:00:00
abstract::To investigate the correlation between the incA I47T mutation in Chlamydia trachomatis and the nonfusogenic phenotype, the incA genes of 25 isolates were sequenced. Four major sequence types were identified. Seven isolates (28%) had the I47T mutation. Isolates representing the four sequence types expressed IncA in the...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.7.4654-4656.2001
更新日期:2001-07-01 00:00:00
abstract::Coxiella burnetii is the causative agent of Q fever, a zoonotic disease that threatens both human and animal health. Due to the paucity of experimental animal models, little is known about how host factors interface with bacterial components and affect pathogenesis. Here, we used Drosophila melanogaster, in conjunctio...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00218-17
更新日期:2017-06-20 00:00:00
abstract::Streptococcus pneumoniae is a major cause of illness and death in children in developing countries. In these children, zinc deficiency is associated with an increased risk of acute respiratory tract infections, which can be reduced by daily zinc administration. Severe infections decrease zinc levels in plasma and may ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.4.2009-2013.2003
更新日期:2003-04-01 00:00:00
abstract::A clone expressing a 58-kDa protein reactive with human convalescent-phase serum was isolated from a recombinant library of Ehrlichia chaffeensis, the etiologic agent of human ehrlichiosis. Sequencing identified two open reading frames, one encoding a 10.3-kDa polypeptide consisting of 94 amino acids and another encod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.61.8.3536-3539.1993
更新日期:1993-08-01 00:00:00
abstract::At least five proteins are secreted extracellularly by enteropathogenic Escherichia coli (EPEC), a leading cause of infant diarrhea in developing countries. However only one, EspC, is known to be secreted independently of the type III secretion apparatus encoded by genes located within the 35.6-kb locus of enterocyte ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.1.315-324.2001
更新日期:2001-01-01 00:00:00
abstract::Chlamydia trachomatis serovar L2 is an obligate intracellular bacterium which is internalized in target epithelial cells by endocytosis and resides within a membrane-bound vesicle. Over the next several hours following entry, individual serovar L2-containing vesicles fuse with one another to form a single membrane-bou...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.4.1208-1214.1996
更新日期:1996-04-01 00:00:00
abstract::Fourteen freshly isolated strains of Streptococcus sanguis were obtained from the dental plaque of five healthy adults. Whole saliva was collected concomitant with the plaque isolates from the five subjects, and a second whole saliva sample was collected 10 weeks later. All possible combinations of the first five sali...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.3.1104-1110.1979
更新日期:1979-12-01 00:00:00
abstract::We have previously shown that a pneumococcal surface protein A (PspA)-based vaccine containing DNA plasmid encoding the Flt3 ligand (FL) gene (pFL) as a nasal adjuvant prevented nasal carriage of Streptococcus pneumoniae. In this study, we further investigated the safety and efficacy of this nasal vaccine for the indu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01360-10
更新日期:2011-07-01 00:00:00
abstract::Elevated levels of immunoglobulin E anti-Candida antibodies were observed in the sera of patients with systemic and vaginal candidiasis and in cervicovaginal washings of the latter. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.1.257-259.1977
更新日期:1977-10-01 00:00:00
abstract::Bordetella pertussis extract that contained adenylate cyclase toxin produced large increases in human neutrophil cyclic AMP levels and inhibited their oxidative burst, as reflected by luminol-enhanced chemiluminescence and superoxide release. The adenylate cyclase toxin-containing extract blocked neutrophil-mediated i...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.56.4.751-755.1988
更新日期:1988-04-01 00:00:00
abstract::Two fragments with molecular weights of 111,000 (fragment I) and 59,000 (fragment II) were separated from each other by gel filtration of dithiothreitol and urea-treated, trypsinized derivative toxin (molecular weight, 170,000) of the proteolytic Okra strain of Clostridium botulinum type B on a column of Sephadex G-20...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.18.3.761-766.1977
更新日期:1977-12-01 00:00:00
abstract::Twenty-six Pseudomonas aeruginosa strains from patients with cystic fibrosis were typed by the Fisher immunotyping scheme. Only 6 strains were agglutinated by a single typing serum, whereas 15 strains were agglutinated with more than one serum and 5 were not agglutinated by any serum. Neither the polyagglutinable nor ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.42.1.170-177.1983
更新日期:1983-10-01 00:00:00
abstract::Cell wall proteins with sialidase activity are involved in carbohydrate assimilation, adhesion to mucosal surfaces, and biofilm formation. Gardnerella spp. inhabit the human vaginal microbiome and encode up to three sialidase enzymes, two of which are suspected to be cell wall associated. Here we demonstrate that the ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00583-20
更新日期:2020-12-23 00:00:00
abstract::Secretion of immunoglobulin A1 (IgA1) proteases is a characteristic of Haemophilus influenzae and several other bacterial pathogens causing infectious diseases, including meningitis. Indirect evidence suggests that the proteases are important virulence factors. In this study, we cloned the iga gene encoding immunoglob...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.10.3097-3105.1989
更新日期:1989-10-01 00:00:00
abstract::Neutrophil extracellular traps (NETs) are produced by neutrophils as an innate immune defense mechanism to trap and kill microbial pathogens. NETs are comprised of ejected chromatin that forms a lattice structure enmeshed with numerous antimicrobial proteins. In addition to forming the structural backbone of NETs, ext...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00403-18
更新日期:2018-08-22 00:00:00
abstract::Saccharomyces boulardii is a nonpathogenic yeast used in the treatment of Clostridium difficile diarrhea and colitis. We have reported that S. boulardii inhibits C. difficile toxin A enteritis in rats by releasing a 54-kDa protease which digests the toxin A molecule and its brush border membrane (BBM) receptor (I. Cas...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.1.302-307.1999
更新日期:1999-01-01 00:00:00
abstract::B-cell-deficient mice were prepared by administration of rabbit anti-mouse-mu antiserum to newborn animals within 12 h of birth onwards. Such immunodeficient animals, along with the normal controls, were infected intravenously with Cryptococcus neoformans. There was no difference in the mortality pattern, viable count...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.26.1.1-3.1979
更新日期:1979-10-01 00:00:00
abstract::The Toll-like receptor 2 (TLR2)/TLR1 receptor complex responds to amyloid fibrils, a common component of biofilm material produced by members of the phyla Firmicutes, Bacteroidetes, and Proteobacteria. To determine whether this TLR2/TLR1 ligand stimulates inflammatory responses when bacteria enter intestinal tissue, w...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00911-12
更新日期:2012-12-01 00:00:00
abstract::Ferrets which had been orally dosed with 5 mg of Staphylococcal enterotoxin B (SEB) responded with an increase in subcutaneous temperature. At 75 min, the subcutaneous temperature was significantly higher (+ 0.9 degrees C +/- 0.38 degrees C, P < 0.007) than in control animals. Animals dosed with 1 or 2 mg of SEB respo...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.4.2386-2389.2000
更新日期:2000-04-01 00:00:00
abstract::Bartonella henselae is a recently recognized pathogenic bacterium associated with cat scratch disease, bacillary angiomatosis, and bacillary peliosis. This study describes the cloning, sequencing, and characterization of an antigenic autotransporter gene from B. henselae. A cloned 6.0-kb BclI-EcoRI DNA fragment expres...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00533-07
更新日期:2007-11-01 00:00:00
abstract::An improved enterocyte adhesion assay has been used to examine a collection of 44 strains of enterotoxigenic Escherichia coli (ETEC) for their ability to adhere to the brush border of isolated human duodenal enterocytes. Fourteen strains showed good adhesion; in each case the ability to adhere correlated with the prod...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.48.3.824-831.1985
更新日期:1985-06-01 00:00:00
abstract::Guinea pig and mouse peritoneal macrophages in culture transport glucose by a specific, saturable system with characteristics compatible with facilitated diffusion. Phagocytosis of killed staphylococci or polystyrene latex spheres results in a significant increase in uptake of 2-deoxy-d-glucose. Reciprocal plot analys...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.10.6.1391-1396.1974
更新日期:1974-12-01 00:00:00
abstract::Oxygenated fatty acids, or oxylipins, play an essential role in physiological signaling and developmental processes in animals, plants, and fungi. Previous characterization of three Aspergillus fumigatus dioxygenases (PpoA, PpoB, and PpoC), similar in sequence to mammalian cyclooxygenases, showed that PpoA is responsi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00009-08
更新日期:2008-07-01 00:00:00
abstract::EspC is an autotransporter protein secreted by enteropathogenic Escherichia coli (EPEC). The pathogenic role of EspC in EPEC infection is unknown. We have shown that the purified EspC produces enterotoxicity and cytotoxicity; for the latter effect, EspC must be internalized. However, the internalization mechanism is u...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.4.2293-2303.2006
更新日期:2006-04-01 00:00:00
abstract::The genome of Borrelia burgdorferi, the etiologic agent of Lyme disease, is composed of a linear chromosome and more than 20 linear and circular plasmids. Typically, plasmid content analysis has been carried out by pulsed-field gel electrophoresis and confirmed by Southern hybridization. However, multiple plasmids of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.3699-3706.2003
更新日期:2003-07-01 00:00:00
abstract::Vaccination against Brucella infections in animals is usually performed by administration of live attenuated smooth B. abortus strain S19 and B. melitensis strain Rev1. They are proven effective vaccines against B. abortus in cattle and against B. melitensis and B. ovis in sheep and goats, respectively. However, both ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.7.4198-4204.2005
更新日期:2005-07-01 00:00:00
abstract::The ability of oral immunization to interfere with the establishment of infection with Helicobacter felis was examined. Groups of Swiss Webster mice were immunized orally with 250 micrograms of Helicobacter pylori recombinant urease (rUrease) and 10 micrograms of cholera toxin (CT) adjuvant, 1 mg of H. felis sonicate ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.63.4.1246-1252.1995
更新日期:1995-04-01 00:00:00