Abstract:
:At least five proteins are secreted extracellularly by enteropathogenic Escherichia coli (EPEC), a leading cause of infant diarrhea in developing countries. However only one, EspC, is known to be secreted independently of the type III secretion apparatus encoded by genes located within the 35.6-kb locus of enterocyte effacement pathogenicity island. EspC is a member of the autotransporter family of proteins, and the secreted portion of the molecule is 110 kDa. Here we determine that the espC gene is located within a second EPEC pathogenicity island at 60 min on the chromosome of E. coli. We also show that EspC is an enterotoxin, indicated by rises in short-circuit current and potential difference in rat jejunal tissue mounted in Ussing chambers. In addition, preincubation with antiserum against the homologous Pet enterotoxin of enteroaggregative E. coli eliminated EspC enterotoxin activity. Like the EAF plasmid, the espC pathogenicity island was found only in a subset of EPEC, suggesting that EspC may play a role as an accessory virulence factor in some but not all EPEC strains.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Mellies JL,Navarro-Garcia F,Okeke I,Frederickson J,Nataro JP,Kaper JBdoi
10.1128/IAI.69.1.315-324.2001keywords:
subject
Has Abstractpub_date
2001-01-01 00:00:00pages
315-24issue
1eissn
0019-9567issn
1098-5522journal_volume
69pub_type
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journal_title:Infection and immunity
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journal_title:Infection and immunity
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