Abstract:
:The possibility that preexisting specific serum antibody could suppress a defined mucosal immune response to a topically applied antigen was studied in rats. Hyperimmune serum antibody induced by parenteral immunization of rats with cholera toxoid markedly suppressed the mucosal immune response to enterically applied cholera toxin. Such antibody was far more suppressive than antibody induced by primary parenteral immunization, apparently due to its greater avidity. Transfusion of small amounts (25 to 100 microliter) of hyperimmune serum suppressed the primary mucosal antitoxin response, the development of specific memory in the mucosal immune system, and, somewhat less effectively, the secondary mucosal antitoxin response. Suppression was due largely to a direct effect of serum antibody upon the interaction of absorbed enteric antigen with lymphoid tissue in Peyer's patches and, possible, mesenteric lymph nodes; interference with antigen absorption played little or no role in the observed suppression. These results do not explain the previously reported suppressive effect of primary parenteral immunization on the mucosal immune response to cholera toxin. However, they support the notions that repeated parenteral immunization can evoke avid serum antibody without necessarily stimulating mucosa-associated lymphoid tissue and that such antibody can markedly suppress primary and secondary phases of the local immune response to mucosally applied antigen. Thus, a mechanism is demonstrated by which repeated parenteral immunization may adversely affect efforts to initiate or sustain protective mucosal immune responses.
journal_name
Infect Immunjournal_title
Infection and immunityauthors
Pierce NFdoi
10.1128/IAI.30.1.62-68.1980subject
Has Abstractpub_date
1980-10-01 00:00:00pages
62-8issue
1eissn
0019-9567issn
1098-5522journal_volume
30pub_type
杂志文章abstract::Bordetella parapertussis, a respiratory tract pathogen commonly regarded as noninvasive, was found to invade HeLa 229 cell monolayers. Following treatment of the monolayers with gentamicin, numbers of viable B. parapertussis recovered were comparable to those of invasive Salmonella and Shigella isolates. Invasion occu...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.4.1240-1247.1989
更新日期:1989-04-01 00:00:00
abstract::Knowledge of the in vivo physiology and metabolism of Streptococcus pneumoniae is limited, even though pneumococci rely on efficient acquisition and metabolism of the host nutrients for growth and survival. Because the nutrient-limited, hypoxic host tissues favor mixed-acid fermentation, we studied the role of the pne...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00178-09
更新日期:2009-12-01 00:00:00
abstract::We undertook a morphological and immunochemical comparison of purified outer membrane antigens from oral and nonoral isolates of Bacteroides asaccharolyticus. Electron micrographs of thin sections of whole bacteria revealed a compact, electron-dense capsule external to the outer membrane of oral strains. A loose, web-...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.27.2.589-596.1980
更新日期:1980-02-01 00:00:00
abstract::Streptococcus pneumoniae is a frequent colonizer of the upper respiratory tract and a leading cause of bacterial pneumonia. The innate immune system senses pneumococcal cell wall components, toxin, and nucleic acids, which leads to production of inflammatory mediators to initiate and control antibacterial defense. Her...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00849-17
更新日期:2018-02-20 00:00:00
abstract::Cholera toxin (CT) and related Escherichia coli enterotoxins LTI and LTIIb have a conserved hydrophobic region at the AB(5) interface postulated to be important for toxin assembly. Hydrophobic residue F223 in the A subunit of CT (CTA) as well as residues 174, L77, and T78 in the B subunit of CT (CTB) were replaced ind...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.7.4093-4101.2003
更新日期:2003-07-01 00:00:00
abstract::Staphylococcus aureus is a pathogen that causes significant morbidity and mortality. Nasal carriage is a major source of transmission and of endogenous infection. Persistent carriage is detected in ∼30% of healthy individuals. While Th17 cells have been shown to play a role in S. aureus infection and clearance, the im...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00834-17
更新日期:2018-02-20 00:00:00
abstract::Localized juvenile periodontosis is associated with a defect of neutrophil chemotaxis that is characterized by selective depletion of a surface protein with a molecular mass of 110 kDa (GP110). Data on partial characterization of GP110 suggest that it is a glycoprotein which is enriched in Lys, Glu, His, Leu, and Ala ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.60.7.3015-3016.1992
更新日期:1992-07-01 00:00:00
abstract::Sterile sonic-treated materials of pneumococci grown in media containing N-acetylmannosamine as the carbohydrate source were lethal for mice. Purification of the toxic factor led to its identification as the enzyme neuraminidase. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.2.1.115-117.1970
更新日期:1970-07-01 00:00:00
abstract::Spotted fever group rickettsiae are known to produce distinct plaque phenotypes. Strains that cause lytic infections in cell culture form clear plaques, while nonlytic strains form opaque plaques in which the cells remain intact. Clear plaques have historically been associated with more-virulent species or strains of ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00048-11
更新日期:2011-04-01 00:00:00
abstract::Several early nonspecific host defense mechanisms were examined in resistant (BALB/c) and susceptible (C3H/He) mice after intraperitoneal inoculation with Rickettsia tsutsugamushi strain Gilliam. Inflammatory exudates were formed in both mouse strains in response to rickettsial inoculation, but the inflammatory respon...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.31.3.1239-1250.1981
更新日期:1981-03-01 00:00:00
abstract::Chromosomes from Cryptococcus neoformans, an encapsulated yeast pathogen, were separated by contour-clamped homogeneous field gel electrophoresis. Seven strains representing all four serotypes were studied. It was found that each strain had a unique, reproducible pattern of chromosome bands which could potentially be ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.9.2624-2627.1989
更新日期:1989-09-01 00:00:00
abstract::Although there appears to be little if any role for specific antibodies in protection against intracellular bacteria, such as the model pathogen F. tularensis live vaccine strain (LVS), the role of B cells themselves in primary and secondary infection with such bacteria has not been examined directly. We show here tha...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.67.11.6002-6007.1999
更新日期:1999-11-01 00:00:00
abstract::Two proteins, HifD and HifE, have been identified as structural components of Haemophilus influenzae pili. Both are localized at the pilus tip, and HifE appears to mediate pilus adherence to host cells. In this study we examined the immunologic and structural diversity of these pilus subunits among type b H. influenza...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.66.10.4788-4796.1998
更新日期:1998-10-01 00:00:00
abstract::Dentilisin is a major surface protease and virulence factor of the bacterium Treponema denticola. In this study, we found that T. denticola reduced inflammatory cytokines, including interleukin-1beta (IL-1beta), IL-6, and tumor necrosis factor alpha, in peripheral blood mononuclear cells through degradation by dentili...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.4.2462-2467.2006
更新日期:2006-04-01 00:00:00
abstract::Borrelia burgdorferi sensu stricto is the major causative agent of Lyme disease in the United States, while B. garinii and B. afzelii are more prevalent in Europe. The highly complex genome of B. burgdorferi is comprised of a linear chromosome and a large number of variably sized linear and circular plasmids. Many pla...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.06326-11
更新日期:2012-04-01 00:00:00
abstract::A new strategy for rapidly selecting and testing genetic vaccines has been developed, in which a whole genome library is cloned into a bacteriophage lambda ZAP Express vector which contains both prokaryotic (P(lac)) and eukaryotic (P(CMV)) promoters upstream of the insertion site. The phage library is plated on Escher...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.74.1.167-174.2006
更新日期:2006-01-01 00:00:00
abstract::Bacterial recognition of host sialic acid-containing receptors plays an important role in microbial colonization of the human oral cavity. The sialic acid-binding adhesin of Streptococcus gordonii DL1 was previously associated with the hsa gene encoding a 203-kDa protein. The predicted protein sequence consists of an ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.72.7.3876-3882.2004
更新日期:2004-07-01 00:00:00
abstract::The stress-induced extracytoplasmic sigma factor E (SigE) of Mycobacterium tuberculosis shows increased expression after heat shock, sodium dodecyl sulfate treatment, and oxidative stress, as well as after phagocytosis in macrophages. We report that deletion of sigE results in delayed lethality in mice without a signi...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.71.12.7170-7172.2003
更新日期:2003-12-01 00:00:00
abstract::The expression of T cell differentiation markers is known to increase during Mycobacterium tuberculosis infection, and yet the biological role of such markers remains unclear. We examined the requirement of the T cell differentiation marker killer cell lectin-like receptor G1 (KLRG1) during M. tuberculosis infection u...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.01199-12
更新日期:2013-04-01 00:00:00
abstract::Salmonella is a widespread zoonotic enteropathogen that causes gastroenteritis and fatal typhoidal disease in mammals. During systemic infection of mice, Salmonella enterica serovar Typhimurium resides and replicates in macrophages within the "Salmonella-containing vacuole" (SCV). It is surprising that the substrates ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00093-09
更新日期:2009-07-01 00:00:00
abstract::Sephadex G-200 filtration was used for fractionating dengue type 2 (DEN-2)-infected suckling mouse brain (SMB) supernatant fluids. The high-molecular-weight fraction, which was eluted in the void volume, showed no increase in type specificity when tested against immune ascitic fluid to the four prototype dengue viruse...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.6.6.952-957.1972
更新日期:1972-12-01 00:00:00
abstract::Exposure to bacterial superantigens such as staphylococcal enterotoxin B (SEB) leads to the induction of toxic shock syndrome which results in multiorgan failure, including liver damage. In the present study, we investigated the role of CD44 in SEB-induced liver injury. Injection of SEB into d-galactosamine-sensitized...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.73.1.50-61.2005
更新日期:2005-01-01 00:00:00
abstract::Accurate diagnosis and early treatment of tuberculosis (TB) and latent TB infection (LTBI) are vital to prevent and control TB. The lack of specific biomarkers hinders these efforts. This study's purpose was to screen immunological markers that discriminate Mycobacterium tuberculosis infection outcomes in a setting wh...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.00759-17
更新日期:2018-03-22 00:00:00
abstract::A multigene family of 58- to 60-kDa proteins, which are designated rhoptry-associated protein 1 (RAP-1) and which come from the parasites Babesia bigemina and Babesia bovis, is a target for vaccine development. The presence of multiple gene copies and conserved sequences and epitopes of RAP-1 implies that these protei...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.64.6.2079-2087.1996
更新日期:1996-06-01 00:00:00
abstract::Gender is a significant factor in determining the susceptibility to and severity of pulmonary diseases in both humans and animals. Murine respiratory mycoplasmosis (MRM), due to Mycoplasma pulmonis infection, is an excellent animal model for evaluation of the role of various host factors on the development of acute or...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.69.5.2865-2871.2001
更新日期:2001-05-01 00:00:00
abstract::Levels of interferon in adult bovine serum and in fetal bovine serum and tissues were examined during the course of transplacental bovine viral diarrhea virus infection. The cows produced circulating interferon between 2 and 9 days after viral inoculation, with mean peak levels in the serum on day 4. Interferon could ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.14.3.660-666.1976
更新日期:1976-09-01 00:00:00
abstract::Isoelectric focusing and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) with acrylamide copolymerized with gelatin (substrate-SDS-PAGE) were combined to evaluate the proteinases of both long-term-grown and fresh isolates of Trichomonas vaginalis. This two-dimensional substrate-SDS-PAGE resolved a...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.58.1.157-162.1990
更新日期:1990-01-01 00:00:00
abstract::We have determined the nucleotide sequence of the 1.4-kilobase DNA fragment containing the plasminogen activator gene (pla) of Yersinia pestis, which determines both plasminogen activator and coagulase activities of the species. The sequence revealed the presence of a 936-base-pair open reading frame that constitutes ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.57.5.1517-1523.1989
更新日期:1989-05-01 00:00:00
abstract::The effect of human-specific antibody on surface membrane antigens of trypomastigotes of Trypanosoma cruzi was studied in vitro by using immunofluorescence methods. Immune sera induced aggregation of surface antigens in trypomastigotes to form polar cell caps. ...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/IAI.20.2.567-569.1978
更新日期:1978-05-01 00:00:00
abstract::Infection by Shigella flexneri is characterized by infiltration of neutrophils in the intestinal mucosa and by a strong inflammatory reaction. Although neutrophils are constitutively programmed to die by apoptosis, we show that isolated human neutrophils undergo necrosis 2 h after infection with virulent S. flexneri s...
journal_title:Infection and immunity
pub_type: 杂志文章
doi:10.1128/iai.68.3.1289-1296.2000
更新日期:2000-03-01 00:00:00