Cholera holotoxin assembly requires a hydrophobic domain at the A-B5 interface: mutational analysis and development of an in vitro assembly system.

abstract：:At least five proteins are secreted extracellularly by enteropathogenic Escherichia coli (EPEC), a leading cause of infant diarrhea in developing countries. However only one, EspC, is known to be secreted independently of the type III secretion apparatus encoded by genes located within the 35.6-kb locus of enterocyte ...

journal_title：Infection and immunity

authors： Mellies JL,Navarro-Garcia F,Okeke I,Frederickson J,Nataro JP,Kaper JB

更新日期：2001-01-01 00:00:00

abstract：:The biological activity of C-ASWS, an alkali-soluble, water-soluble cell wall antigen isolated from mycelial-phase cells of Coccidioides immitis, was compared with that of a commercial coccidioidin (CDN; Cutter Laboratories); CDN-TS, a toluene-induced lysate of mycelial-phase cells; and spherulin, a spherule-derived e...

journal_title：Infection and immunity

authors： Cox RA,Brummer E,Lecara G

更新日期：1977-03-01 00:00:00

abstract：:A total of five bacteriological samples from the periodontal pockets of two sisters with localized juvenile periodontitis and their mother with advanced periodontitis was studied. Gram-negative anaerobic rods were predominant in the samples. Bacteroides intermedius and Bacteroides loescheii were the most predominant s...

journal_title：Infection and immunity

authors： Okuda K,Naito Y,Ohta K,Fukumoto Y,Kimura Y,Ishikawa I,Kinoshita S,Takazoe I

更新日期：1984-07-01 00:00:00

abstract：:Mast cell-deficient W/Wv mice and their mast cell-sufficient littermates were given infections of Trichinella spiralis. W/Wv mice were slower than their littermates to expel adult T. spiralis. Repair of the mast cell deficiency of W/Wv mice by bone marrow grafting was accompanied by accelerated expulsion of T. spirali...

journal_title：Infection and immunity

authors： Ha TY,Reed ND,Crowle PK

更新日期：1983-07-01 00:00:00

abstract：:Human colostrum, parotid saliva, and serum were assayed for the presence of naturally occurring antibodies to five serotypes of Streptococcus mutans. Appreciable levels of agglutinins to strains AHT, BHT, 10449, 6715, and LM-7 (groups a leads to e, respectively) were detected in normal colostrum and saliva, whereas re...

journal_title：Infection and immunity

authors： Arnold RR,Mestecky J,McGhee JR

更新日期：1976-08-01 00:00:00

abstract：:In an attempt to clarify the mechanism(s) of increased susceptibility to oral infection in diabetics, we examined the levels of salivary antibacterial factors, including lysozyme, lactoperoxidase, and lactoferrin, in diabetic hamsters whose condition was induced with streptozotocin. Saliva was collected from these ham...

journal_title：Infection and immunity

authors： Muratsu K,Morioka T

更新日期：1985-05-01 00:00:00

abstract：:To analyze the role of interleukin-10 (IL-10) in bacterial cerebral infections, we studied cerebral listeriosis in IL-10-deficient (IL-10(-/-)) and wild-type (WT) mice, the latter of which express high levels of IL-10 in both primary and secondary cerebral listeriosis. IL-10(-/-) mice succumbed to primary as well as s...

journal_title：Infection and immunity

authors： Deckert M,Soltek S,Geginat G,Lütjen S,Montesinos-Rongen M,Hof H,Schlüter D

更新日期：2001-07-01 00:00:00

abstract：:We evaluated sexual recombination in the apicomplexan parasite Theileria parva using genome-wide marker analysis of haploid sporozoite populations obtained from infected Rhipicephalus appendiculatus ticks. Analysis of 231 parasite clones derived by in vitro infection of bovine lymphocytes revealed 48 distinct combinat...

journal_title：Infection and immunity

authors： Katzer F,Ngugi D,Oura C,Bishop RP,Taracha EL,Walker AR,McKeever DJ

更新日期：2006-10-01 00:00:00

abstract：:Passive protection, the administration of antibodies to prevent infection, has garnered significant interest in recent years as a potential prophylactic countermeasure to decrease the prevalence of hospital-acquired infections. Pili, polymerized protein structures covalently anchored to the peptidoglycan wall of many ...

journal_title：Infection and immunity

authors： Pinkston KL,Singh KV,Gao P,Wilganowski N,Robinson H,Ghosh S,Azhdarinia A,Sevick-Muraca EM,Murray BE,Harvey BR

更新日期：2014-04-01 00:00:00

abstract：:Cellular resistance to facultative intracellular parasites has been studied by determining the antimycobacterial activity and the amount of fatty acids in sera and in heptane extracts of freshly collected and 24-h-cultured normal and activated guinea pig alveolar macrophages and liver cells. The quantity and the antim...

journal_title：Infection and immunity

authors： Hemsworth GR,Kochan I

更新日期：1978-01-01 00:00:00

abstract：:Phospholipase A2 and lysophospholipase activities were detected in the culture supernatant fluids of a virulent strain of Vibrio vulnificus. The phospholipase A2 was inactivated by heating at 56 degrees C for 30 min, had an apparent molecular weight of greater than or equal to 80,000 (estimated by gel filtration with ...

journal_title：Infection and immunity

authors： Testa J,Daniel LW,Kreger AS

更新日期：1984-08-01 00:00:00

abstract：:Whole blood and plasma from animals in the acute stage of leptospirosis contained a toxic factor which produced a cytopathic effect on fibroblastic L cell monolayers. Firm adsorption of cytotoxic factor (CTF) to L cells occurred within 1 h. The highest titer of CTF in plasma was reached at 24 h and declined after 48 h...

journal_title：Infection and immunity

authors： Knight LL,Miller NG,White RJ

更新日期：1973-09-01 00:00:00

abstract：:Murine hybridoma cell lines were developed which synthesized monoclonal antibodies against Actinobacillus actinomycetemcomitans-associated antigens. Monoclonal antibodies specific for an antigen(s) common to all A. actinomycetemcomitans isolates tested but not detected on other gram-negative oral plaque microorganisms...

journal_title：Infection and immunity

authors： Place DA,Scidmore NC,McArthur WP

更新日期：1988-05-01 00:00:00

abstract：:Genetically based natural resistance to brucellosis in cattle provides for novel strategies to control zoonotic diseases. Bovine NRAMP1, the homologue of a murine gene (Bcg), has been identified as a major candidate for controlling the in vivo resistant phenotype. We developed an in vitro model for expression of resis...

journal_title：Infection and immunity

authors： Barthel R,Feng J,Piedrahita JA,McMurray DN,Templeton JW,Adams LG

更新日期：2001-05-01 00:00:00

abstract：:We assessed cell-mediated immunity to herpes simplex virus type 1 antigen in patients suffering from recurrent cold sores and in a series of healthy controls. Paradoxically, all those subject to recurrent herpetic infections had, without exception, evidence of cell-mediated immunity to herpes antigens. This was demons...

journal_title：Infection and immunity

authors： Russell AS,Percy JS,Kovithavongs T

更新日期：1975-02-01 00:00:00

abstract：:Female mice infected at birth with 10(7) 50% tissue culture infective doses of polyoma virus were mated when at least 6 weeks old. Polyoma was not detected in any tissues of 27 female mice before mating except for trace amounts in the kidneys of 2 mice, but late in gestation polyoma virus could be found in the kidneys...

journal_title：Infection and immunity

authors： McCance DJ,Mims CA

更新日期：1979-09-01 00:00:00

abstract：:Pretreatment with multiple doses of polymyxin B and colistimethate was evaluated as to its ability to sequester sufficient antibiotic in tissues to neutralize the effects of endotoxin in three animal models. Animals were challenged with endotoxin 24, 48, or 72 h after the last dose of antibiotic when there was minimal...

journal_title：Infection and immunity

authors： Craig WA,Turner JH,Kunin CM

更新日期：1974-08-01 00:00:00

abstract：:Analysis of the specificity of bactericidal antibodies in normal, convalescent, and postvaccination human sera is important in understanding human immunity to meningococcal infections and can aid in the design of an effective group B vaccine. A collection of human sera, including group C and group B convalescent-phase...

journal_title：Infection and immunity

authors： Schmiel DH,Moran EE,Keiser PB,Brandt BL,Zollinger WD

更新日期：2011-10-01 00:00:00

abstract：:The humoral and cell-mediated immune responses of murine lymphocytes exposed to lead in vivo and in vitro were investigated. In vivo Pb was administered via the drinking water (0 to 10 mM) for 1 to 10 weeks. In vivo exposure of the mice to Pb did not alter significantly their plaque-forming cell response to sheep eryt...

journal_title：Infection and immunity

authors： Lawrence DA

更新日期：1981-01-01 00:00:00

abstract：:Numerous bacterial pathogens use type III secretion systems (T3SSs) or T4SSs to inject or translocate virulence proteins into eukaryotic cells. Several different reporter systems have been developed to measure the translocation of these proteins. In this study, a peptide tag-based reporter system was developed and use...

journal_title：Infection and immunity

authors： Garcia JT,Ferracci F,Jackson MW,Joseph SS,Pattis I,Plano LR,Fischer W,Plano GV

更新日期：2006-10-01 00:00:00

abstract：:The Mycobacterium tuberculosis exported repetitive protein (Erp) is a virulence determinant required for growth in cultured macrophages and in vivo. To better understand the role of Erp in Mycobacterium pathogenesis, we generated a mutation in the erp homologue of Mycobacterium marinum, a close genetic relative of M. ...

journal_title：Infection and immunity

authors： Cosma CL,Klein K,Kim R,Beery D,Ramakrishnan L

更新日期：2006-06-01 00:00:00

abstract：:Binding of Escherichia coli strain 431 heat-stable enterotoxin (STa) and activation of intestinal particulate guanylate cyclase by E. coli STa were studied with rat intestinal epithelial cells and brush border membranes (BBMs). The rates of guanylate cyclase stimulation by 431 STa in cells and BBMs were rapid, with ma...

journal_title：Infection and immunity

authors： Dreyfus LA,Robertson DC

更新日期：1984-11-01 00:00:00

abstract：:Whooping cough results from infection of the respiratory tract with Bordetella pertussis, and the secreted adenylate cyclase toxin (ACT) is essential for the bacterium to establish infection. Despite extensive study of the mechanism of ACT cytotoxicity and its effects over a range of concentrations in vitro, ACT has n...

journal_title：Infection and immunity

authors： Eby JC,Gray MC,Warfel JM,Paddock CD,Jones TF,Day SR,Bowden J,Poulter MD,Donato GM,Merkel TJ,Hewlett EL

更新日期：2013-05-01 00:00:00

abstract：:Active immunization with Porphyromonas gingivalis whole-cell preparations has been shown to prevent P. gingivalis infection and oral bone loss. Employing passive antibody transfer and opsonization, we demonstrate with this study that immunization-elicited P. gingivalis-specific immunoglobulin G facilitates clearance o...

journal_title：Infection and immunity

authors： Gibson FC 3rd,Gonzalez DA,Wong J,Genco CA

更新日期：2004-04-01 00:00:00

abstract：:We found that thiols reduced the amount of cholera toxin produced by Vibrio cholerae 569B in vitro. A sulfhydryl group at least was necessary for the reduction of cholera toxin production by thiols. ...

journal_title：Infection and immunity

authors： Shimamura T,Watanabe S,Sasaki S

更新日期：1986-09-01 00:00:00

abstract：:As an intracellular protozoan parasite, Toxoplasma gondii is likely to exploit proteases for host cell invasion, acquisition of nutrients, avoidance of host protective responses, escape from the parasitophorous vacuole, differentiation, and other activities. T. gondii serine protease inhibitor 1 (TgPI1) is the most ab...

journal_title：Infection and immunity

authors： Pszenny V,Davis PH,Zhou XW,Hunter CA,Carruthers VB,Roos DS

更新日期：2012-03-01 00:00:00

abstract：:The lipid A portion has been identified as the active center responsible for lipopolysaccharide (LPS)-induced macrophage activation. However, we found that Salmonella (Salmonella enterica serovars Abortusequi, Minnesota, and Typhimurium) lipid A is inactive in human macrophages, despite its LPS being highly active. Th...

journal_title：Infection and immunity

authors： Muroi M,Tanamoto K

更新日期：2002-11-01 00:00:00

abstract：:Invasive fungal infections represent an increasing problem associated with high mortality. The present study was undertaken to identify leukocyte subsets that are activated by hyphal fragments in a whole-human-blood model, as well as to examine the involvement of CD14 and Toll-like receptors (TLRs) in activation of mo...

journal_title：Infection and immunity

authors： Wang JE,Warris A,Ellingsen EA,Jørgensen PF,Flo TH,Espevik T,Solberg R,Verweij PE,Aasen AO

更新日期：2001-04-01 00:00:00

abstract：:In this study, the efficacy of bactericidal/permeability-increasing protein (BPI) was assessed in a rat model of chronic otitis media with effusion. BPI injection prevented disturbance of the mucociliary clearance system of the middle ear. Hence, it is postulated that BPI can be a new therapy for chronic otitis media ...

journal_title：Infection and immunity

authors： Nell MJ,Koerten HK,Grote JJ

更新日期：2000-05-01 00:00:00

abstract：:A persistent Staphylococcus epidermidis infection in mice around a subcutaneous polyvinylpyrrolidone-grafted silicon elastomer catheter (SEpvp) but not around a conventional silicon elastomer catheter was observed. With SEpvp pericatheter tissue, protracted and exaggerated interleukin-1beta (IL-1beta) production was f...

journal_title：Infection and immunity

authors： Boelens JJ,Zaat SA,Murk JL,Weening JJ,van Der Poll T,Dankert J

更新日期：2000-03-01 00:00:00